{"title":"Non-destructive estimation of the amount of green fluorescent protein transiently expressing in Nicotiana benthamiana leaves with a viral vector","authors":"Ryo MATSUDA, Shinya URAYAMA, Fumiki HOSOI, Kazuhiro FUJIWARA","doi":"10.2480/agrmet.d-23-00024","DOIUrl":"https://doi.org/10.2480/agrmet.d-23-00024","url":null,"abstract":"</p><p> A custom-made fluorescence measurement system was constructed for non-destructive and quantitative estimation of green fluorescent protein (GFP) transiently expressing in a <i>Nicotiana benthamiana</i> leaf with a viral vector. The system comprised a digital camera, a blue LED lamp, and a long wavelength-pass filter to acquire the brightness intensity of the green channel (<i>G</i> value) of a leaf surface image as a measure of fluorescence intensity. The genes encoding GFP and tobamoviral vector were transferred to <i>N. benthamiana</i> leaves 42 days post seeding by syringe infiltration with <i>Agrobacterium</i> for transient expression. The destructively measured GFP content in leaves as the ground-truth data started to increase at 3-4 days post infiltration (DPI), once showed a plateau at 6-8 DPI, and then increased again until 11 DPI. Regression analysis demonstrated that the <i>r</i><sup>2</sup> of the linear regression between the ground-truth GFP content and the <i>G</i> value was 0.86-0.87 when the <i>G</i> value at a given DPI was calibrated with the initial leaf color at 0 DPI for the same leaf. These results indicate that the constructed system and method could potentially be used to quantitatively and non-destructively estimate the amount of GFP transiently expressing in intact <i>N. benthamiana</i> leaves.</p>\u0000<p></p>","PeriodicalId":56074,"journal":{"name":"Journal of Agricultural Meteorology","volume":"56 1","pages":""},"PeriodicalIF":1.3,"publicationDate":"2024-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139409828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jean Yves DUKUZUMUREMYI, Samuel Munyaka KIMANI, Weiguo CHENG, Christian NKURUNZIZA, Margi Asih MAIMUNAH, Eric CANTONA, Shuirong TANG, Shigeto SUDO, Yuka SASAKI, Hideki MURAYAMA
{"title":"No-weeding suppressed methane emissions by Takanari and Koshihikari rice varieties under organic farming","authors":"Jean Yves DUKUZUMUREMYI, Samuel Munyaka KIMANI, Weiguo CHENG, Christian NKURUNZIZA, Margi Asih MAIMUNAH, Eric CANTONA, Shuirong TANG, Shigeto SUDO, Yuka SASAKI, Hideki MURAYAMA","doi":"10.2480/agrmet.d-23-00031","DOIUrl":"https://doi.org/10.2480/agrmet.d-23-00031","url":null,"abstract":"</p><p> To determine the difference in greenhouse gas emissions between two rice (<i>Oryza sativa</i> L.) varieties; Takanari (Tak) and Koshihikari (Kos), under organic rice cultivation with or without weeds management, a pot experiment was conducted at Tsuruoka, Yamagata, Japan. The Tak and Kos varieties were transplanted individually and interplanted as Tak+Kos, with the main treatments of weeding (w) and no-weeding (nw). Methane (CH<sub>4</sub>), nitrous oxide (N<sub>2</sub>O), and plant night respiration (CO<sub>2</sub> emission) fluxes were measured once weekly or biweekly during the rice growth period. As the results, N<sub>2</sub>O fluxes were not detected but CH<sub>4</sub> fluxes varied with the rice growth stage for all treatments. Compared with the Kos(w), Tak(w) and Tak+Kos(w) significantly decreased total seasonal CH<sub>4</sub> emissions by 12.4% and 19.1%, respectively. This was attributed to higher rhizospheric CH<sub>4</sub> oxidation and the offset of potential methanogenic activities because of the larger root biomass and a higher harvest index for both Tak(w) and Tak+Kos(w) compared with Kos(w). Conversely, Kos(nw) significantly reduced cumulative CH<sub>4</sub> emissions by 31.7% and 48.6% compared with Tak(nw) and Tak+Kos(nw), respectively. This was ascribed to high rhizospheric CH<sub>4</sub> oxidation promoted by the presence of significantly higher weed biomass for Kos(nw) at 65.0% and 44.0% compared with Tak(nw) and Tak+Kos(nw), respectively. On average, the no-weeding practice significantly decreased cumulative CH<sub>4</sub> emissions by 50.5% compared with the weeding practice, with a significantly high (<i>P</i><0.001) interaction between weed management and variety differences observed. The CH<sub>4</sub> emissions and plant biomass under variety interplanting (Tak+Kos) were not significantly different to Tak individual planting, but were significantly different to Kos individual planting, which implied variety Tak had strong competition to variety Kos. More studies on appropriate variety choice and pairs, and the effect of aquatic weeds presence in no-weeding organic paddy fields are necessary to estimate CH<sub>4</sub> emission and attenuation.</p>\u0000<p></p>","PeriodicalId":56074,"journal":{"name":"Journal of Agricultural Meteorology","volume":"32 1","pages":""},"PeriodicalIF":1.3,"publicationDate":"2024-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139409696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Comparisons of different sample air-drying systems for carbon dioxide flux measurements based on eddy covariance in cold environments","authors":"Taichi Noshiro, Daiki Nomura, Fumiyoshi Kondo, Keisuke Ono, Brent Else, Hiroki Ikawa","doi":"10.2480/agrmet.d-23-00014","DOIUrl":"https://doi.org/10.2480/agrmet.d-23-00014","url":null,"abstract":"","PeriodicalId":56074,"journal":{"name":"Journal of Agricultural Meteorology","volume":"47 8","pages":""},"PeriodicalIF":1.3,"publicationDate":"2024-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139440880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Preferable weather patterns to brown planthopper advection to Kyushu and its effect of climate change","authors":"Keito OISHI, Masaru INATSU, Sho KAWAZOE","doi":"10.2480/agrmet.d-23-00022","DOIUrl":"https://doi.org/10.2480/agrmet.d-23-00022","url":null,"abstract":"</p><p> The brown planthopper, <i>Nilaparvata lugens</i> (Stål), a type of rice pest, immigrates from southern China to western Japan by drifting in the southwesterly environment during the Baiu/Meiyu rainy season. This study aims to investigate the preferable weather patterns for the immigration of brown planthoppers across the East China Sea. We conducted immigration runs using an advection diffusion model for brown planthoppers and projected the possibility of them landing in Kyushu in the model run onto 64 weather map patterns typically observed in June and July, identified by the self-organizing map analysis. The results showed that the immigration occurred under two specific weather map patterns: flow stagnation around western Japan and a low-level jet blowing over the East China Sea. Consistency between landing cases in model runs and the occurrence of the two weather patterns was found in the intraseasonal and interannual variability. We also estimated the change in the frequency of brown planthopper arrival in Kyushu based on the climate change dataset, the database for Policy Decision Making for Future climate change (d4PDF). It was found that the flow stagnation patterns increased in response to global change, at least in the d4PDF dataset. Finally, risk assessment for temperature change and a comparison with trap observations were discussed.</p>\u0000<p></p>","PeriodicalId":56074,"journal":{"name":"Journal of Agricultural Meteorology","volume":"1 1","pages":""},"PeriodicalIF":1.3,"publicationDate":"2024-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139409791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"A monitoring system for evaluating the responses in CH4 uptake to rising CH4 concentrations in forest soils based on a closed chamber system","authors":"C. Kikuchi, M. Ueyama, S. Takanashi","doi":"10.2480/agrmet.d-23-00013","DOIUrl":"https://doi.org/10.2480/agrmet.d-23-00013","url":null,"abstract":"","PeriodicalId":56074,"journal":{"name":"Journal of Agricultural Meteorology","volume":" ","pages":""},"PeriodicalIF":1.3,"publicationDate":"2023-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42835086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Determinants of genotypic variations in the yield response of soybean to elevated atmospheric CO2","authors":"E. Kumagai, H. Shimono","doi":"10.2480/agrmet.d-23-00009","DOIUrl":"https://doi.org/10.2480/agrmet.d-23-00009","url":null,"abstract":"","PeriodicalId":56074,"journal":{"name":"Journal of Agricultural Meteorology","volume":" ","pages":""},"PeriodicalIF":1.3,"publicationDate":"2023-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43121264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
T. Nakano, Y. Kurosaki, Takehiko Y. Ito, Y. Iijima, Ganbat Bavuudorj
{"title":"Applicability of digital camera images to estimate vegetation parameters in semi-arid grasslands of Mongolia","authors":"T. Nakano, Y. Kurosaki, Takehiko Y. Ito, Y. Iijima, Ganbat Bavuudorj","doi":"10.2480/agrmet.d-23-00008","DOIUrl":"https://doi.org/10.2480/agrmet.d-23-00008","url":null,"abstract":"","PeriodicalId":56074,"journal":{"name":"Journal of Agricultural Meteorology","volume":"1 1","pages":""},"PeriodicalIF":1.3,"publicationDate":"2023-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42056449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Lineage switch of KMT2A-rearranged adult B-lineage acute lymphoblastic leukemia following bispecific T-cell engager and monoclonal antibody therapy.","authors":"Jia-Rong Wu, Pei-Chun Shih, Ching Li, Hsiao-Ling Chao, Hsiao-Chun Wang, Yi-Mei Chiang, Yu-Jung Liu, Szu-Chun Hsu, Chi-Yuan Yao, Lo-Ho Chen, Chien-Chin Lin, Hwei-Fang Tien, Wen-Chien Chou","doi":"10.1007/s12308-023-00539-6","DOIUrl":"10.1007/s12308-023-00539-6","url":null,"abstract":"<p><p>Adult B-lineage acute lymphoblastic leukemia (B-ALL) with t(4;11)(q21;q23) is very rare. It is characterized by mixed-lineage leukemia and has the potential for lineage switching during the treatment course. We report the disease course of a patient with B-ALL with t(4;11)(q21;q23) to demonstrate that close monitoring of cell morphology and immunophenotyping is necessary to capture the lineage switch at an early stage. Cell morphology, immunophenotyping, and cytogenetics were used to evaluate the patient's disease status. A 36-year-old woman was diagnosed with B-ALL with t(4;11)(q21;q23), which encodes the KMT2A::AFF1 fusion. After the initial induction chemotherapy, her disease remained refractory, and the patient received salvage immunotherapy with blinatumomab and inotuzumab ozogamicin. However, the ALL did not respond. Repeated bone marrow examinations unexpectedly revealed the emergence of a major population of monoblasts, in addition to a minor population of the original B lymphoblasts. The patient was diagnosed with disease evolution from B-ALL to mixed-phenotype acute leukemia (MPAL, B/myeloid). We present this case to highlight the potential of KMT2A-rearranged B-ALL to undergo lineage switch following B-cell targeted therapy. Patients with this kind of B-ALL should therefore be closely monitored to capture potential changes in the nature of the disease and prompt appropriate treatment.</p>","PeriodicalId":56074,"journal":{"name":"Journal of Agricultural Meteorology","volume":"72 1","pages":"103-109"},"PeriodicalIF":0.6,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91155932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The effect of periodical grass mowing and various meteorological factors on CO2 flux in a sod-cultured apple orchard","authors":"D. Ito, S. Ishida","doi":"10.2480/agrmet.d-22-00010","DOIUrl":"https://doi.org/10.2480/agrmet.d-22-00010","url":null,"abstract":"","PeriodicalId":56074,"journal":{"name":"Journal of Agricultural Meteorology","volume":" ","pages":""},"PeriodicalIF":1.3,"publicationDate":"2023-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42225212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effects of farmyard manure application and grassland renovation on net ecosystem carbon balance in a temperate grassland: analysis of 11-year eddy covariance data","authors":"S. Matsuura, Akinori Mori, A. Miyata, R. Hatano","doi":"10.2480/agrmet.d-22-00007","DOIUrl":"https://doi.org/10.2480/agrmet.d-22-00007","url":null,"abstract":"","PeriodicalId":56074,"journal":{"name":"Journal of Agricultural Meteorology","volume":" ","pages":""},"PeriodicalIF":1.3,"publicationDate":"2023-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49047284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}