Mikrobiologia i Biotehnologia最新文献

{"title":"DEEPENED GENOMICS-METABOLOMICS CHARACTERISTICS OF THE BACTERIA OF THE BACILLUS GENUS ISOLATED FROM DEEP-SEA","authors":"V. V. Subota, A. M. Ostapchuk, М. D. Shtenikov","doi":"10.18524/2307-4663.2023.2(58).287369","DOIUrl":"https://doi.org/10.18524/2307-4663.2023.2(58).287369","url":null,"abstract":"Marine bacteria from the genus Bacillus are attracting increasing attention as a source of large amounts of bioactive metabolites. The biosynthetic potential of marine bacteria from the genus Bacillus is not sufficiently studied. The previous study of authors could not give a complete result, so it was decided to reanalyze the metabolites not identified at that time, taking into account the development of existing databases. Therefore, the work aimed to reanalyze the metabolome already performed on Bacillus velezensis ONU 553, Bacillus pumilus ONU 554, and Bacillus subtilis ONU 559 strains isolated from the Black Sea sediments. Methods. The general genome identification was performed using the online version of the Dictionary of Natural Products database, and search for biosynthetic clusters in genome of Bacillus velezensis ONU 553 - with antiSMASH 7.0. Results and Conclusion. Genomic-metabolic profiling of strains led to the identification of several new biological compounds – 7 and 1 metabolites in Bacillus velezensis ONU 553 and Bacillus pumilus ONU 554 strains, respectively, and to identify biosynthetic clusters of iturin- and helipeptin-like peptides in Bacillus velezensis ONU 553 strain for the first time. A new biosynthetic cluster was found in the Bacillus velezensis ONU 553 strain.","PeriodicalId":55835,"journal":{"name":"Mikrobiologia i Biotehnologia","volume":"100 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135587206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MORPHOLOGICAL CHARACTERISTICS AND CYTOTOXIC ACTIVITY OF THE BLACK SEA STRAIN STREPTOMYCES SP. LIM 10","authors":"T. V. Gudzenko, I. V. Strashnova, O. G. Gorshkova, G. V. Lisiutin, M. D. Shtenikov, V. I. Sachkovska","doi":"10.18524/2307-4663.2023.2(58).286962","DOIUrl":"https://doi.org/10.18524/2307-4663.2023.2(58).286962","url":null,"abstract":"Aim. The aim of the work was to study the morphological characteristics and cytotoxic activity of the strain of actinobacteria Streptomyces sp. Lim 10 isolated from natural fouling of shell rock in the Odesa Bay of the Black Sea. Materials and methods. The object of the study was a strain of marine actinobacteria Streptomyces sp. Lim 10. The morphology and growth patterns were studied on the media of Gauze 2, Czapek, Eshbi, TSB and on the media of ISP (ISP-1–ISP-7). SG medium was used to accumulate secondary metabolites of actinobacteria. Extraction of exometabolites from the culture liquid was carried out with ethyl acetate followed by dissolution in dimethyl sulfoxide (DMSO). The cytotoxic activity of exometabolites of actinobacteria strain Streptomyces sp. Lim 10 was studied on the model of cultures cells human - rhabdomyosarcoma (RD,) and of the laryngeal adenocarcinoma (Hep-2). The cytotoxic effect of exometabolites of the actinobacteria strain Streptomyces sp. Lim 10 was determined after 24 hours of incubation by microscopy. The degree of destruction of the monolayer was assessed by the number of viable cells in terms of optical density, which was measured using a spectrophotometer at a wavelength of 630 nm. The exometabolites of actinobacteria strain Streptomyces sp. Lim 10 were identified using LC-ESIMS. Results. Study of the morphological properties of the Black Sea strain of actinobacteria Lim 10 showed that it is characterized by pleomorphism. А high cytotoxic activity of exometabolites of the marine strain of actinobacteria Streptomyces sp. Lim 10 was established. The level of cytotoxic effect of metabolites of the strain of actinobacteria Streptomyces sp. Lim 10 on Hep-2 cell culture depended on their concentration. RD cell culture was more sensitive to the cytotoxic effect of exometabolites of Streptomyces sp. Lim 10 – at all tested concentrations of exometabolites (from 2.5 to 500.0 μg/ml), the number of viable cells in the RD monolayer decreased to 51.3 ± 3.0 – 63.7 ± 5.4%, compared to the control. This can be at least partially explained by action of apoptosis-inducing agent staurosporine, which is produced by strain Streptomyces sp. Lim 10 along with itsstructural homologs. Conclusion. Strain Streptomyces sp. Lim 10 is a promising producer of antitumor compounds and can be recommended for further more in-depth studies.","PeriodicalId":55835,"journal":{"name":"Mikrobiologia i Biotehnologia","volume":"87 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135587078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"INFLUENCE OF COMBINED PHARMACOPROPHYLAXIS OF INFECTIOUS DISEASES ON THE CONDITION OF THE GASTROINTESTINAL TRACT OF LABORATORY RATS","authors":"B. М. Galkin, T. V. Hladkii, N. A. Kyrylenko","doi":"10.18524/2307-4663.2023.2(58).287865","DOIUrl":"https://doi.org/10.18524/2307-4663.2023.2(58).287865","url":null,"abstract":"The aim of the work was studying the possibility of combined pharmacoprophylaxis of infectious diseases during the use of antibiotics in laboratory animals by applying a complex of medicines, including vitamins and minerals. Methods. The experiment was performed on 16 rats aged 1.5 months: 8 – intact, 8 – pharmacoprophylaxis (Baytril Bayer 10 mg/kg; ascorbic acid 2 g/l; glucose solution 50 g/l; Chiktonic 1 ml/l; Baycox 3 ml/l) within 24 days. For biochemical studies, the liver, mucous membranes of the stomach, small and large intestine were isolated, in which indicators of inflammation (activity of elastase and acid phosphatase), the state of non-specific antimicrobial protection (activity of lysozyme), indicator of microbial insemination (activity of urease) were determined. Results. Pharmacoprophylaxis according to the scheme in the mucous membranes of the digestive tract of rats led to an increase in markers of inflammation (activity of acid phosphatase and elastase), development of dysbiosis (increase in urease activity against the background of decrease in lysozyme activity). More significant changes were registered in the mucous membrane of the large intestine. In the liver of rats, an increase in the activity of elastase, acid phosphatase and urease was established along with decrease in the activity of lysozyme, which indicates the presence of inflammation and a violation of the antitoxic and antimicrobial functions of the liver. Conclusion. The study showed the insufficiency of the use of vitamin-mineral complex, ascorbic acid and glucose as protective components of the digestive tract against the negative effects of antibiotics. This dictates the need to find more effective protective agents, capable of preventing the negative effects of antibiotics.","PeriodicalId":55835,"journal":{"name":"Mikrobiologia i Biotehnologia","volume":"36 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135587375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"IMPROVEMENT OF THE CHRYSANTHEMUM × KOREANUM HORT. CLONAL MICROPROPAGATION PROCESSES USING BACILLUS MEGATERIUM ONU500","authors":"N. I. Tesliuk, O. Y. Zinchenko, M. B. Galkin, T. O. Filipova","doi":"10.18524/2307-4663.2023.2(58).287368","DOIUrl":"https://doi.org/10.18524/2307-4663.2023.2(58).287368","url":null,"abstract":"The aim of this study was to improve the Chrysanthemum × Koreanum micropropagation processes using Bacillus megaterium ONU500 at the post-aseptic adaptation stage. Methods. At the initial stages of clonal micropropagation, two surface sterilization protocols with different fungicidal preparations were tested. The influence of the agar concentration in the medium on the development of explants was determined. At the ex vitro adaptation stage, the roots of the microclones were inoculated with bacteria of the strain B. megaterium ONU500. Results. The most effective protocol for plant material surface disinfection, which included quinozol and ethanol, was established. It was determined that the use of Murashige and Skoog (MS) nutrient medium supplemented with 0.4% agar at the stage of in vitro culture establishment increased the survival of chrysanthemum initial explants by 9.3% and accelerated proliferation by 1 day. It was found that inoculation of the chrysanthemum microclone rhizosphere with bacteria had a positive effect on the growth of adapted plantlets, increasing their survival rate by 20.4–22.2%, shoot height by 1.8–1.9 cm, and average number of nodes by 2.0–2.2 nodes. Conclusion. The effectiveness of the use of fungicides for the treatment of chrysanthemum plant material during in vitro culture establishment and the influence of the consistency of the nutrient medium on the development of explants were determined, which is new and relevant for increasing the efficiency of clonal micropropagation of this plant. Also, experiments on the inoculation of chrysanthemum microclones at the stage of ex vitro adaptation were carried out for the first time, and the positive effect of such inoculation on the morphometric characteristics of seedlings and their survival rate was shown. When establishing chrysanthemum explants in vitro, it is recommended to use the fungicide quinozol for surface sterilization, and carry out cultivation in a semi-liquid nutrient medium with 0.4% agar. At the postaseptic adaptation stage of microclones, it is advisable to inoculate the roots with bacteria of the strain B. megaterium ONU500 at concentrations of 108–109 CFU/ml.","PeriodicalId":55835,"journal":{"name":"Mikrobiologia i Biotehnologia","volume":"55 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135587075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ANTIMICROBIAL ACTIVITY OF SOIL BACILLI AGAINST PHYTOPATHOGENIC MICROORGANISMS ISOLATED FROM AFFECTED CEREAL PLANTS","authors":"I. V. Strashnova, O. V. Andriuschenko","doi":"10.18524/2307-4663.2023.2(58).286959","DOIUrl":"https://doi.org/10.18524/2307-4663.2023.2(58).286959","url":null,"abstract":"Aim of this study was to investigate the antagonistic effect of bacilli isolated from the surface of plants and the rhizosphere zone of the soil against pathogens of barley and wheat diseases. Materials and methods. The material for the study was affected barley and wheat samples used to isolate phytopathogenic microorganisms, as well as samples of healthy plants and the rhizosphere soil zone used to isolate bacteria of the genus Bacillus. Manipulations for the isolation and investigation the biological properties of both phytopathogens and bacilli were carried out by traditional microbiological methods. The antagonistic activity of the isolated strains of bacilli against phytopathogens was studied by the block method. Results. From samples of affected wheat and barley were isolated microorganisms identified on the basis of the studied biological properties as Fusarium oxysporum, Sclerotinia sclerotiorum, Xanthomonas arboricola and Pectobacterium carotovorum. The largest proportion of strains was represented by the micromycete Fusarium oxysporum – 53.9%. From samples of the rhizosphere soil zone and leaf-stem mass of healthy wheat and barley plants, 86 strains of bacteria of the genus Bacillus were isolated by microbiological methods. Antagonistic activity was inherent in 54 strains of bacilli against 25% of phytopathogens. All pathogenic microorganisms were inhibited with different intensity by 14 strains of bacilli. Conclusion. The most active bacillus strains (Bacillus spp. 6, 9, 13, 21, 50) were selected for further research.","PeriodicalId":55835,"journal":{"name":"Mikrobiologia i Biotehnologia","volume":"15 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135587208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}