DEEPENED GENOMICS-METABOLOMICS CHARACTERISTICS OF THE BACTERIA OF THE BACILLUS GENUS ISOLATED FROM DEEP-SEA

Marine bacteria from the genus Bacillus are attracting increasing attention as a source of large amounts of bioactive metabolites. The biosynthetic potential of marine bacteria from the genus Bacillus is not sufficiently studied. The previous study of authors could not give a complete result, so it was decided to reanalyze the metabolites not identified at that time, taking into account the development of existing databases. Therefore, the work aimed to reanalyze the metabolome already performed on Bacillus velezensis ONU 553, Bacillus pumilus ONU 554, and Bacillus subtilis ONU 559 strains isolated from the Black Sea sediments. Methods. The general genome identification was performed using the online version of the Dictionary of Natural Products database, and search for biosynthetic clusters in genome of Bacillus velezensis ONU 553 - with antiSMASH 7.0. Results and Conclusion. Genomic-metabolic profiling of strains led to the identification of several new biological compounds – 7 and 1 metabolites in Bacillus velezensis ONU 553 and Bacillus pumilus ONU 554 strains, respectively, and to identify biosynthetic clusters of iturin- and helipeptin-like peptides in Bacillus velezensis ONU 553 strain for the first time. A new biosynthetic cluster was found in the Bacillus velezensis ONU 553 strain.