Fungal EcologyPub Date : 2025-06-21DOI: 10.1016/j.funeco.2025.101439
Álvaro López-García , Hans Henrik Bruun , Jing Tang , Rasmus Kjøller , Søren Rosendahl
{"title":"Assembly of arbuscular mycorrhizal fungal communities changes from stochastic to deterministic during primary succession","authors":"Álvaro López-García , Hans Henrik Bruun , Jing Tang , Rasmus Kjøller , Søren Rosendahl","doi":"10.1016/j.funeco.2025.101439","DOIUrl":"10.1016/j.funeco.2025.101439","url":null,"abstract":"<div><div>On new land, succession of arbuscular mycorrhizal (AM) fungal communities depends on dispersal of propagules from a regional metacommunity and on supply of plant partners locally. We followed the succession of AM fungal communities from 2010 to 2016 on the artificial island Peberholm, which was constructed in 1998. We surveyed AM fungal communities using metabarcoding. Early in succession, AM fungal communities were characterized by a high proportion of sporulating taxa and strong dominance, consistent with a dispersal-driven mass effect imprinting the initial community composition. Over time, the prevalence of sporulating taxa declined, additional taxa were gained and β-diversity at the whole-island level increased. This pattern suggest a gradual shift towards a more deterministic assembly, where biotic interactions and environmental filtering play a greater role, leading to higher spatial differentiation of AM communities in response to abiotic conditions and plant communities.</div></div>","PeriodicalId":55136,"journal":{"name":"Fungal Ecology","volume":"77 ","pages":"Article 101439"},"PeriodicalIF":1.9,"publicationDate":"2025-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144330758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Fungal EcologyPub Date : 2025-06-20DOI: 10.1016/j.funeco.2025.101441
Shannon L. Sharpe, Dale Casamatta, Anthony Rossi
{"title":"Endophytic fungal diversity within galls induced by Asphondylia borrichiae (Diptera: Cecidomyiidae) and their surrounding host tissues (Borrichia frutescens and Iva frutescens)","authors":"Shannon L. Sharpe, Dale Casamatta, Anthony Rossi","doi":"10.1016/j.funeco.2025.101441","DOIUrl":"10.1016/j.funeco.2025.101441","url":null,"abstract":"<div><div><em>Asphondylia borrichiae</em> gall midges deposit endophytic fungi (EF) during oviposition. However, their fungal associations, EF diversity across host plants, and potential interactions between them all remain poorly understood. Galls and tissues from two hosts (<em>Borrichia frutescens</em> and <em>Iva frutescens</em>) were surveyed to catalog and compare EF diversity. Molecular sequencing (ITS 1–4) and culturing identified 21 fungal genera: 95.6 % Ascomycota and 4.4 % Basidiomycota. <em>Fusarium</em> spp. were isolated from all sample sets (36–63 % relative abundance), while <em>Botryosphaeria dothidea</em> (9–11 %), <em>Cladosporium</em> sp. (11–13 %), <em>Alternaria</em> sp. (4 %), and <em>Clonostachys</em> sp. (2–6 %) were most abundant in gall samples. <em>Botryosphaeria dothidea</em> was found in both gall-lining and non-gall tissues of <em>B. frutescens</em>. Gall EF diversity was significantly higher in <em>B. frutescens</em> (p < 0.000573). Future research should explore how host-switching or post-associational barriers might influence EF transmission or communities. Further understanding these associations will inform broader studies on EF distributions and myco-phytophagous insect life cycles.</div></div>","PeriodicalId":55136,"journal":{"name":"Fungal Ecology","volume":"77 ","pages":"Article 101441"},"PeriodicalIF":1.9,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144321903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Fungal EcologyPub Date : 2025-06-03DOI: 10.1016/j.funeco.2025.101442
Ayuka Iwakiri , Tsubasa Nakagawa , Norihisa Matsushita , Roger T. Koide , Yasushi Hashimoto
{"title":"Sources of variation in foliar endophytic fungal communities in a unique bog system, Hokkaido, Japan","authors":"Ayuka Iwakiri , Tsubasa Nakagawa , Norihisa Matsushita , Roger T. Koide , Yasushi Hashimoto","doi":"10.1016/j.funeco.2025.101442","DOIUrl":"10.1016/j.funeco.2025.101442","url":null,"abstract":"<div><div>Plants harbor diverse foliar endophytic fungi (FEF). Host species is a major determinant of FEF community structure. When considering the host effect on FEF communities, however, it is often challenging to minimize the effects from the variability of other biotic and abiotic environmental factors. In this study, we focused on a bog system to study the host–FEF community relationship, where four woody plant species of almost identical size are codistributed on small hummocks. FEF community structure did significantly differ among the four host species. It was also suggested that host phylogenetic relatedness may partially explain FEF community structure. Indicator species analysis indicated that around 10 % OTUs were host-specific, and analyses using FungalTraits showed that most are plant pathogens. Overall, foliar endophytic fungi appear to be filtered by host species, but determining the ecological roles of host-specific FEF will require further study.</div></div>","PeriodicalId":55136,"journal":{"name":"Fungal Ecology","volume":"77 ","pages":"Article 101442"},"PeriodicalIF":1.9,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144195344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Fungal EcologyPub Date : 2025-05-15DOI: 10.1016/j.funeco.2025.101438
Joanne E. Taylor , Karsten Schönrogge , Anna Oliver , Stephen Cavers , Joan Cottrell , Annika Perry , Melanie Gibbs , Richard Ennos
{"title":"Effects of fungicide application on the foliar endophytic fungi of Scots pine (Pinus sylvestris)","authors":"Joanne E. Taylor , Karsten Schönrogge , Anna Oliver , Stephen Cavers , Joan Cottrell , Annika Perry , Melanie Gibbs , Richard Ennos","doi":"10.1016/j.funeco.2025.101438","DOIUrl":"10.1016/j.funeco.2025.101438","url":null,"abstract":"<div><div>Following fungicide treatment of young Scots pine trees in Scotland targeted at <em>Dothistroma septosporum</em>, foliar fungal endophytic communities were investigated with culture-based methods and metabarcoding of cDNA. Compared to negative controls, application of fungicides resulted in significant reductions in the size and diversity of endophytic communities with alterations to their taxonomic composition. While most taxa showed reductions in frequency and abundance across samples, <em>Anthostomella pinea</em> and a <em>Preussia</em> sp. temporarily increased. Thirteen taxa were identified using culturing, compared to 569 by metabarcoding (with 41 taxa accounting for 89.4 % of the total reads). <em>Dothistroma septosporum</em> and the reportedly unculturable <em>Lophodermella conjuncta</em> both contributed significantly to differences observed using metabarcoding but occurred at low levels or were absent, respectively, in the culture-based study. The persistent effects we observed of fungicides on the endophytic fungal community have possible practical implications for management of tree seedlings in the nursery.</div></div>","PeriodicalId":55136,"journal":{"name":"Fungal Ecology","volume":"76 ","pages":"Article 101438"},"PeriodicalIF":1.9,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143948749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Fungal EcologyPub Date : 2025-05-12DOI: 10.1016/j.funeco.2025.101440
Michael Köhler , Georg Hähn , Maarten Kanitz , Olga Ferlian , Nico Eisenhauer , Tesfaye Wubet , Helge Bruelheide
{"title":"The effects of tree diversity and neighborhood on phyllosphere fungal communities","authors":"Michael Köhler , Georg Hähn , Maarten Kanitz , Olga Ferlian , Nico Eisenhauer , Tesfaye Wubet , Helge Bruelheide","doi":"10.1016/j.funeco.2025.101440","DOIUrl":"10.1016/j.funeco.2025.101440","url":null,"abstract":"<div><div>Phyllosphere fungi, encompassing both epiphytic and endophytic fungi, have rarely been studied along gradients of host diversity. In this study, we used a tree biodiversity experiment to investigate how tree species richness, host and neighboring species identities, and host-mycorrhizal association affect the richness and composition of the whole phyllosphere community as well as that considering epiphytes and endophytes separately. To distinguish epiphytes from endophytes, we employed a leaf-surface sterilization treatment. Richness and composition of the mycobiome were quantified using next-generation amplicon sequencing. Our findings revealed that tree species richness affected only the overall phyllosphere fungal community, not endophytes alone, suggesting that neighborhood effects predominantly influence fungi on the leaf cuticle. Neighborhood effects were only detectable in the phyllosphere as a whole and not within endophyte and epiphyte communities. Fungal community composition was shaped by host species identity, tree richness, host mycorrhizal type and the combination of mycorrhizal types at the plot level. This study underlines the importance of analyzing epiphytes and endophytes separately and highlights the necessity of using leaf-surface sterilization when examining phyllosphere fungal communities.</div></div>","PeriodicalId":55136,"journal":{"name":"Fungal Ecology","volume":"76 ","pages":"Article 101440"},"PeriodicalIF":1.9,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143936087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Fungal EcologyPub Date : 2025-04-26DOI: 10.1016/j.funeco.2025.101436
Peter G. Avis , Lucia Liet Goldman , Joseph E. Carrara , Ivan Fernandez
{"title":"Six years later: Ectomycorrhizal fungal communities recovering after the end of long-term nitrogen and sulfur addition in a mixed-species temperate North American forest","authors":"Peter G. Avis , Lucia Liet Goldman , Joseph E. Carrara , Ivan Fernandez","doi":"10.1016/j.funeco.2025.101436","DOIUrl":"10.1016/j.funeco.2025.101436","url":null,"abstract":"<div><div>We examined how ectomycorrhizal (ECM) fungal communities of a North American mixed species temperate forest recovered from 26 years of experimental nitrogen and sulfur addition. We used Illumina-based metabarcoding of the ribosomal DNA ITS region to compare the ECM fungal communities in soil samples collected at the end (in 2016) and six years after (in 2022) the conclusion of the Bear Brook Watershed Experiment, Maine, USA, a 26 year ammonium-sulfate addition study conducted in adjacent ∼10 ha treated and reference watersheds. Six years after the experiment ended, ECM fungal species richness in the treated watershed returned to reference watershed levels with a significant increase by <em>Cortinarius</em>, a nitrophobic genus. In addition, the ECM fungal community composition of the treated watershed was significantly different six years after the experiment ended. Because this forest is in a region of historically low nitrogen deposition, this study establishes a baseline for the study of recovery in other regions where nitrogen deposition is higher and more widespread.</div></div>","PeriodicalId":55136,"journal":{"name":"Fungal Ecology","volume":"76 ","pages":"Article 101436"},"PeriodicalIF":1.9,"publicationDate":"2025-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143874195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Fungal EcologyPub Date : 2025-04-24DOI: 10.1016/j.funeco.2025.101437
Yu Fukasawa , Kristin Aleklett Kadish
{"title":"The emergence of fungal ethology as a distinctive discipline in mycology","authors":"Yu Fukasawa , Kristin Aleklett Kadish","doi":"10.1016/j.funeco.2025.101437","DOIUrl":"10.1016/j.funeco.2025.101437","url":null,"abstract":"","PeriodicalId":55136,"journal":{"name":"Fungal Ecology","volume":"76 ","pages":"Article 101437"},"PeriodicalIF":1.9,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144230977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Fungal EcologyPub Date : 2025-04-24DOI: 10.1016/j.funeco.2025.101435
Yu Fukasawa, Satsuki Kimura
{"title":"Carbon allocation shifts during fungal mycelial competition under the heat stress","authors":"Yu Fukasawa, Satsuki Kimura","doi":"10.1016/j.funeco.2025.101435","DOIUrl":"10.1016/j.funeco.2025.101435","url":null,"abstract":"<div><div>Wood-decaying basidiomycetes play crucial roles in global carbon cycling. The relative dominance of brown rot and white rot fungi, which vary with temperature, is key to understanding carbon sequestration in a changing environment. In this study, we measured changes in carbon allocation between brown and white rot fungi under heat stress to assess the outcome of their competition. After 25 days of incubation in liquid media, heat stress reduced hyphal biomass and increased CO<sub>2</sub> emissions in both <em>Neolentinus</em> (brown rot) and <em>Trichaptum</em> (white rot) strains. However, carbon allocation to metabolite production and unknown fractions shifted markedly in <em>Trichaptum</em> strains under heat stress, whereas changes in <em>Neolentinus</em> strains were less pronounced. Competition between <em>Neolentinus</em> and <em>Trichaptum</em> shifted from a deadlock at optimal temperature to <em>Neolentinus</em>'s dominance under heat stress. These findings suggest that <em>Neolentinus</em> is more heat-tolerant compared to <em>Trichaptum</em>, maintaining more stable metabolic activity under heat stress.</div></div>","PeriodicalId":55136,"journal":{"name":"Fungal Ecology","volume":"76 ","pages":"Article 101435"},"PeriodicalIF":1.9,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143865119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Fungal EcologyPub Date : 2025-04-10DOI: 10.1016/j.funeco.2025.101425
Godar Sene , Chinedu C. Obieze , Obinna T. Ezeokoli , Brian Boyle , Ali M. Quoreshi , Damase P. Khasa
{"title":"Evaluation of soil fungal communities using the ITS2 sublocus and 18S gene primers under different amplification methods","authors":"Godar Sene , Chinedu C. Obieze , Obinna T. Ezeokoli , Brian Boyle , Ali M. Quoreshi , Damase P. Khasa","doi":"10.1016/j.funeco.2025.101425","DOIUrl":"10.1016/j.funeco.2025.101425","url":null,"abstract":"<div><div>Primer selection and PCR methods can potentially lead to biased descriptions of microbial communities. Here, we investigated soil total fungi and arbuscular mycorrhizal fungal (AMF) community composition and diversity from diverse sites using several primers and amplification methods. The results showed that regardless of site, the ITS3tagmix1-5/ITS4ngs primer set generated a higher proportion of high-quality reads, targeted more fungal ASVs and revealed a higher total fungal alpha diversity compared to ITS3tagmix4/ITS4ngs. Among specific primers targeting the 18S rRNA gene, AMV4.5NF/AMDGR had differential specificity for Glomeraceae, whereas SSU515Fngs/Euk742R had differential specificity for Paraglomeraceae. Regardless of site, PCR approaches (nested vs non-nested) had higher influence on the AMF community structure than primer selection, though primer selection significantly influenced arbuscular mycorrhizal fungi richness. Overall, the findings suggest that the specificity of amplification in relation to primer selection and PCR stringency should guide the best interpretation of fungal community diversity data from high-throughput sequencing of environmental samples.</div></div>","PeriodicalId":55136,"journal":{"name":"Fungal Ecology","volume":"76 ","pages":"Article 101425"},"PeriodicalIF":1.9,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143817012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Fungal EcologyPub Date : 2025-04-08DOI: 10.1016/j.funeco.2025.101426
Massimo Cogliati , Sevim Akçağlar , Okan Tore , Tadeja Matos , Rok Tomazin , Irena Zdovc , Donjeta Pllana-Hajdari , Patricia Escandon , Sara Epis , Giulia Maria Cattaneo , Francesca Serio
{"title":"Interactions between the pathogenic fungus Cryptococcus neoformans and ants","authors":"Massimo Cogliati , Sevim Akçağlar , Okan Tore , Tadeja Matos , Rok Tomazin , Irena Zdovc , Donjeta Pllana-Hajdari , Patricia Escandon , Sara Epis , Giulia Maria Cattaneo , Francesca Serio","doi":"10.1016/j.funeco.2025.101426","DOIUrl":"10.1016/j.funeco.2025.101426","url":null,"abstract":"<div><div>Despite the growing number of environmental surveys aimed to understand the ecology of the fungal pathogens <em>Cryptococcus neoformans</em> and <em>Cryptococcus gattii</em>, little is known about their relationships with arthropods. In the present study we collected a large number of samples from trees and arthropods living on them to determine the occurrence of <em>Cryptococcus</em> in arthropods, to understand if they could represent a vehicle for dispersion in the environment, and finally to investigate how they might interact with the fungus. Samples were collected from seven different geographical areas of the world: northwestern Italy, southeastern Italy, Slovenia, Kosovo, Greece, Turkey, and Colombia. A total of 1396 trees were examined and 11,805 samples were collected, including 7492 arthropod samples. Arthropod positive samples, mostly from ants, were found only in northwestern and southeastern Italy, Greece, and Slovenia with an average rate of 0.2%. Thirty-three of positive trees hosted positive arthropods whereas in six of them arthropods resulted negative. In addition, for six trees, positive samples from arthropods were not associated with positive arboreal samples. In vitro experiments showed that ants can transfer cryptococcal yeasts from a contaminated substrate (soil or bark) to a sterile one and that the fungus can survive inside the digestive apparatus of ants. The present study showed that ants are potential vehicles for <em>C. neoformans</em> although the frequency of which they enter in contact with the fungus is low. Cryptococcal yeasts can survive within the bodies of ants, but it remains unclear whether the relationship they establish with their host is parasitic, commensal, or symbiotic.</div></div>","PeriodicalId":55136,"journal":{"name":"Fungal Ecology","volume":"76 ","pages":"Article 101426"},"PeriodicalIF":1.9,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143790850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}