Hfsp Journal最新文献_第9页

Mechanical factors activate beta-catenin-dependent oncogene expression in APC mouse colon. 机械因子激活APC小鼠结肠β -连环蛋白依赖癌基因表达。

Hfsp Journal Pub Date : 2008-10-01 Epub Date: 2008-07-09 DOI: 10.2976/1.2955566

Joanne Whitehead, Danijela Vignjevic, Claus Fütterer, Emmanuel Beaurepaire, Sylvie Robine, Emmanuel Farge

{"title":"Mechanical factors activate beta-catenin-dependent oncogene expression in APC mouse colon.","authors":"Joanne Whitehead, Danijela Vignjevic, Claus Fütterer, Emmanuel Beaurepaire, Sylvie Robine, Emmanuel Farge","doi":"10.2976/1.2955566","DOIUrl":"https://doi.org/10.2976/1.2955566","url":null,"abstract":"beta-catenin acts as a critical regulator of gastrointestinal homeostasis through its control of the Wnt signaling pathway, and genetic or epigenetic lesions which activate Wnt signaling are the primary feature of colon cancer. beta-catenin is also a key element of mechanotranscription pathways, leading to upregulation of master developmental gene expression during Drosophila gastrulation, or regulating mammalian bone development and maintenance. Here we investigate the impact of mechanical stimulation on the initiation of colon cancer. Myc and Twist1, two oncogenes regulated through beta-catenin, are expressed in response to transient compression in APC deficient (APC(1638N+)) colon tissue explants, but not in wild-type colon explants. Mechanical stimulation of APC(1638N+) tissue leads to the phosphorylation of beta-catenin at tyrosine 654, the site of interaction with E-cadherin, as well as to increased nuclear localization of beta-catenin. The mechanical activation of Myc and Twist1 expression in APC(1638N+) colon can be prevented by blocking beta-catenin phosphorylation using Src kinase inhibitors. Microenvironmental signals are known to cooperate with genetic lesions to promote the nuclear beta-catenin accumulation which drives colon cancer. Here we demonstrate that when APC is limiting, mechanical strain, such as that associated with intestinal transit or tumor growth, can be interpreted by cells of preneoplastic colon tissue as a signal to initiate a beta-catenin dependent transcriptional program characteristic of cancer.","PeriodicalId":55056,"journal":{"name":"Hfsp Journal","volume":"2 5","pages":"286-94"},"PeriodicalIF":0.0,"publicationDate":"2008-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.2976/1.2955566","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28141087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 60

Nanoscale hydrodynamics in the cell: balancing motorized transport with diffusion. 细胞内的纳米尺度流体动力学:机动运输与扩散的平衡。

Hfsp Journal Pub Date : 2008-10-01 Epub Date: 2008-09-15 DOI: 10.2976/1.2978984

Robert H Austin

引用次数: 4

Making connections for life: an in vivo map of the yeast interactome. 为生命建立联系:酵母相互作用组的体内图。

Hfsp Journal Pub Date : 2008-10-01 Epub Date: 2008-08-13 DOI: 10.2976/1.2969243

Juergen Kast

{"title":"Making connections for life: an in vivo map of the yeast interactome.","authors":"Juergen Kast","doi":"10.2976/1.2969243","DOIUrl":"https://doi.org/10.2976/1.2969243","url":null,"abstract":"Proteins are the true workhorses of any cell. To carry out specific tasks, they frequently bind other molecules in their surroundings. Due to their structural complexity and flexibility, the most diverse array of interactions is seen with other proteins. The different geometries and affinities available for such interactions typically bestow specific functions on proteins. Having available a map of protein-protein interactions is therefore of enormous importance for any researcher interested in gaining insight into biological systems at the level of cells and organisms. In a recent report, a novel approach has been employed that relies on the spontaneous folding of complementary enzyme fragments fused to two different proteins to test whether these interact in their actual cellular context [Tarassov et al., Science 320, 1465-1470 (2008)]. Genome-wide application of this protein-fragment complementation assay has resulted in the first map of the in vivo interactome of Saccharomyces cerevisiae. The current data show striking similarities but also significant differences to those obtained using other large-scale approaches for the same task. This warrants a general discussion of the current state of affairs of protein-protein interaction studies and foreseeable future trends, highlighting their significance for a variety of applications and their potential to revolutionize our understanding of the architecture and dynamics of biological systems.","PeriodicalId":55056,"journal":{"name":"Hfsp Journal","volume":"2 5","pages":"244-50"},"PeriodicalIF":0.0,"publicationDate":"2008-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.2976/1.2969243","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28141080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Pulsing cells: how fast is too fast? 脉冲细胞:多快算太快?

Hfsp Journal Pub Date : 2008-10-01 Epub Date: 2008-08-26 DOI: 10.2976/1.2969901

Saurabh Paliwal, C Joanne Wang, Andre Levchenko

引用次数: 14

Cell adhesion and response to synthetic nanopatterned environments by steering receptor clustering and spatial location. 细胞粘附和响应合成纳米模式的环境通过转向受体集群和空间定位。

Hfsp Journal Pub Date : 2008-10-01 Epub Date: 2008-09-29 DOI: 10.2976/1.2976662

Elisabetta Ada Cavalcanti-Adam, Daniel Aydin, Vera Catherine Hirschfeld-Warneken, Joachim Pius Spatz

{"title":"Cell adhesion and response to synthetic nanopatterned environments by steering receptor clustering and spatial location.","authors":"Elisabetta Ada Cavalcanti-Adam, Daniel Aydin, Vera Catherine Hirschfeld-Warneken, Joachim Pius Spatz","doi":"10.2976/1.2976662","DOIUrl":"https://doi.org/10.2976/1.2976662","url":null,"abstract":"During adhesion and spreading, cells form micrometer-sized structures comprising transmembrane and intracellular protein clusters, giving rise to the formation of what is known as focal adhesions. Over the past two decades these structures have been extensively studied to elucidate their organization, assembly, and molecular composition, as well as to determine their functional role. Synthetic materials decorated with biological molecules, such as adhesive peptides, are widely used to induce specific cellular responses dependent on cell adhesion. Here, we focus on how surface patterning of such bioactive materials and organization at the nanoscale level has proven to be a useful strategy for mimicking both physical and chemical cues present in the extracellular space controlling cell adhesion and fate. This strategy for designing synthetic cellular environments makes use of the observation that most cell signaling events are initiated through recruitment and clustering of transmembrane receptors by extracellular-presented signaling molecules. These systems allow for studying protein clustering in cells and characterizing the signaling response induced by, e.g., integrin activation. We review the findings about the regulation of cell adhesion and focal adhesion assembly by micro- and nanopatterns and discuss the possible use of substrate stiffness and patterning in mimicking both physical and chemical cues of the extracellular space.","PeriodicalId":55056,"journal":{"name":"Hfsp Journal","volume":"2 5","pages":"276-85"},"PeriodicalIF":0.0,"publicationDate":"2008-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.2976/1.2976662","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28141085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 111

Light sheet-based fluorescence microscopy: more dimensions, more photons, and less photodamage. 基于薄片的荧光显微镜:更多的维度，更多的光子，更少的光损伤。

Hfsp Journal Pub Date : 2008-10-01 Epub Date: 2008-09-15 DOI: 10.2976/1.2974980

Emmanuel G Reynaud, Uros Krzic, Klaus Greger, Ernst H K Stelzer

{"title":"Light sheet-based fluorescence microscopy: more dimensions, more photons, and less photodamage.","authors":"Emmanuel G Reynaud, Uros Krzic, Klaus Greger, Ernst H K Stelzer","doi":"10.2976/1.2974980","DOIUrl":"https://doi.org/10.2976/1.2974980","url":null,"abstract":"Light-sheet-based fluorescence microscopy (LSFM) is a fluorescence technique that combines optical sectioning, the key capability of confocal and two-photon fluorescence microscopes with multiple-view imaging, which is used in optical tomography. In contrast to conventional wide-field and confocal fluorescence microscopes, a light sheet illuminates only the focal plane of the detection objective lens from the side. Excitation is, thus, restricted to the fluorophores in the volume near the focal plane. This provides optical sectioning and allows the use of regular cameras in the detection process. Compared to confocal fluorescence microscopy, LSFM reduces photo bleaching and photo toxicity by up to three orders of magnitude. In LSFM, the specimen is embedded in a transparent block of hydrogel and positioned relative to the stationary light sheet using precise motorized translation and rotation stages. This feature is used to image any plane in a specimen. Additionally, multiple views obtained along different angles can be combined into a single data set with an improved resolution. LSFMs are very well suited for imaging large live specimens over long periods of time. However, they also perform well with very small specimens such as single yeast cells. This perspective introduces the principles of LSFM, explains the challenges of specimen preparation, and introduces the basics of a microscopy that takes advantage of multiple views.","PeriodicalId":55056,"journal":{"name":"Hfsp Journal","volume":"2 5","pages":"266-75"},"PeriodicalIF":0.0,"publicationDate":"2008-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.2976/1.2974980","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28141084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 175

Toward integration of in vivo molecular computing devices: successes and challenges. 迈向体内分子计算装置的整合:成功与挑战。

Hfsp Journal Pub Date : 2008-10-01 Epub Date: 2008-08-13 DOI: 10.2976/1.2968443

Sikander Hayat, Thomas Hinze

引用次数: 5

Acoustic physics of surface-attached biochemical species. 表面附着生物化学物质的声物理。

Hfsp Journal Pub Date : 2008-08-01 Epub Date: 2008-06-23 DOI: 10.2976/1.2938856

Jonathan S Ellis, Michael Thompson

引用次数: 6

Exploring the transport of plant metabolites using positron emitting radiotracers. 利用正电子发射放射性示踪剂探索植物代谢产物的运输。

Hfsp Journal Pub Date : 2008-08-01 Epub Date: 2008-07-08 DOI: 10.2976/1.2921207

Matthew R Kiser, Chantal D Reid, Alexander S Crowell, Richard P Phillips, Calvin R Howell

{"title":"Exploring the transport of plant metabolites using positron emitting radiotracers.","authors":"Matthew R Kiser, Chantal D Reid, Alexander S Crowell, Richard P Phillips, Calvin R Howell","doi":"10.2976/1.2921207","DOIUrl":"10.2976/1.2921207","url":null,"abstract":"Short-lived positron-emitting radiotracer techniques provide time-dependent data that are critical for developing models of metabolite transport and resource distribution in plants and their microenvironments. Until recently these techniques were applied to measure radiotracer accumulation in coarse regions along transport pathways. The recent application of positron emission tomography (PET) techniques to plant research allows for detailed quantification of real-time metabolite dynamics on previously unexplored spatial scales. PET provides dynamic information with millimeter-scale resolution on labeled carbon, nitrogen, and water transport over a small plant-size field of view. Because details at the millimeter scale may not be required for all regions of interest, hybrid detection systems that combine high-resolution imaging with other radiotracer counting technologies offer the versatility needed to pursue wide-ranging plant physiological and ecological research. In this perspective we describe a recently developed hybrid detection system at Duke University that provides researchers with the flexibility required to carry out measurements of the dynamic responses of whole plants to environmental change using short-lived radiotracers. Following a brief historical development of radiotracer applications to plant research, the role of radiotracers is presented in the context of various applications at the leaf to the whole-plant level that integrates cellular and subcellular signals andor controls.","PeriodicalId":55056,"journal":{"name":"Hfsp Journal","volume":"2 4","pages":"189-204"},"PeriodicalIF":0.0,"publicationDate":"2008-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.2976/1.2921207","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28140588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 61

Collective behavior in animal groups: theoretical models and empirical studies. 动物群体中的集体行为:理论模型与实证研究。

Hfsp Journal Pub Date : 2008-08-01 DOI: 10.2976/1.2961038

Irene Giardina

引用次数: 231