Hybridoma最新文献_第3页

Characterization of monoclonal antibodies against carcinoembryonic antigen (CEA) and expression in E. coli. 抗癌胚抗原单克隆抗体的鉴定及在大肠杆菌中的表达。

Hybridoma Pub Date : 2001-08-01 DOI: 10.1089/027245701753179857

S. H. Kim, J. Chun, S. Y. Park

{"title":"Characterization of monoclonal antibodies against carcinoembryonic antigen (CEA) and expression in E. coli.","authors":"S. H. Kim, J. Chun, S. Y. Park","doi":"10.1089/027245701753179857","DOIUrl":"https://doi.org/10.1089/027245701753179857","url":null,"abstract":"Eight monoclonal antibodies (MAbs) against carcinoembryonic antigen (CEA) were characterized. Five clones are IgG(1), two clones are IgM and one clone is IgG(2b); all have kappa light chain. The affinities are in the range of 1.1 x 10(-7) approximately 2.4 x 10(-9) M; the affinities of two IgM clones could not be estimated because of their low enzyme-linked immunoadsorbent assay (ELISA) signal. Each clone was constructed as single-chain Fv (scFv) and expression was performed in E. coli. Four clones out of 8 could express scFv soluble to culture media and the expression was confirmed further by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting. The nucleotide and amino acid sequences of V(H) and V(L) of four scFvs were deduced and their family and subgroup were analyzed. We found that the clones that do not express the scFv have aberrant kappa chain (incorrect V/J recombination or stop codon); in contrast, their heavy chain sequences proved correct. The E. coli-expressed scFvs showed 1.5 x 3.4-fold lower affinities (2.8 x 10(-8) approximately 3.6 x 10(-9) M) than those of hybridoma-derived parental antibodies except the one clone (C5), which exhibited approximately 10(-6) M of affinity.","PeriodicalId":55044,"journal":{"name":"Hybridoma","volume":"20 4 1","pages":"265-72"},"PeriodicalIF":0.0,"publicationDate":"2001-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/027245701753179857","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"60500446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

A monoclonal antibody specific for Pseudomonas aeruginosa amidase. 一种铜绿假单胞菌酰胺酶特异性单克隆抗体。

Hybridoma Pub Date : 2001-08-01 DOI: 10.1089/027245701753179866

C. Novo, A. Karmali, A. Clemente, P. Brown

引用次数: 5

Generation and characterization of monoclonal antibodies against the E6 and E7 oncoproteins of HPV. 人乳头瘤病毒E6和E7癌蛋白单克隆抗体的制备和鉴定。

Hybridoma Pub Date : 2001-08-01 DOI: 10.1089/027245701753179848

A. Wlazlo, W. Giles-Davis, A. Clements, G. Struble, R. Marmorstein, H. Ertl

引用次数: 5

Production, characterization, and epitope mapping of monoclonal antibodies against human polydeoxyribonucleotide kinase. 抗人多脱氧核糖核苷酸激酶单克隆抗体的生产、鉴定和表位定位。

Hybridoma Pub Date : 2001-08-01 DOI: 10.1089/027245701753179811

M. Fanta, H. Zhang, N. Bernstein, M. Glover, Feridoun Karimi-Busheri, M. Weinfeld

引用次数: 13

Evaluation of clinical significance of isoferritin by development of new monoclonal antibodies specific for acidic isoferritin. 酸性异铁蛋白特异性单克隆抗体的研制评价异铁蛋白的临床意义。

Hybridoma Pub Date : 2001-08-01 DOI: 10.1089/027245701753179820

S. You, C. Zhang, X. Yi, W. Huang

{"title":"Evaluation of clinical significance of isoferritin by development of new monoclonal antibodies specific for acidic isoferritin.","authors":"S. You, C. Zhang, X. Yi, W. Huang","doi":"10.1089/027245701753179820","DOIUrl":"https://doi.org/10.1089/027245701753179820","url":null,"abstract":"We produced monoclonal antibodies (MAbs) against acidic isoferritin (AIF) of primary hepatic carcinoma (PHC) and human placental tissues. Competitive enzyme-linked immunosorbent assay (ELISA) revealed that each MAb bound to different antigenic determinants of AIF, but there is the same epitope between PHC AIF and placental AIF. Sandwich-type ELISA was developed to measure the concentration of serum AIF in the patients with PHC, chronic hepatitis, and cirrhosis. In most of cases of PHC, serum AIF levels were found to be significantly elevated, but were in low levels in almost all of the patients with chronic hepatitis and cirrhosis. On the other hand, we have studied the expression of AIF in liver tissue. Immunohistochemical study using MAb4c9 specific for PHC AIF and MAb7a9 specific for placental AIF has shown that AIF positive staining rates of hepatocytes with liver tissues of PHC, nonmalignant live diseases and normal control were 81.6, 6.7, 0%, and 73.7, 10, 0%, respectively. We also studied the p53 protein expression in those liver tissues (47.4, 0, 0% in PHC, nonmalignant liver diseases and normal liver, respectively). Our data indicated that there was significant correlation between AIF and p53 expression in liver tissues of PHC. Taken together, the results suggested that AIF is probably synthesized and secreted by the tumor cells of PHC and its production may reflect carcinogenesis of hepatocytes. Anti-PHC AIF MAb was clearly superior in diagnosis of PHC to antiplacental AIF MAb, and has potential application of immunotherapy.","PeriodicalId":55044,"journal":{"name":"Hybridoma","volume":"20 4 1","pages":"243-8"},"PeriodicalIF":0.0,"publicationDate":"2001-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/027245701753179820","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"60500529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Monoclonal antibodies against human telomerase reverse transcriptase (hTERT): preparation, characterization, and application. 抗人端粒酶逆转录酶(hTERT)单克隆抗体:制备、表征和应用。

Hybridoma Pub Date : 2001-08-01 DOI: 10.1089/027245701753179839

S. Yang, B. Zhang, J. Wang, S. Liao, J. Han, J. Wei, L. Hou

引用次数: 10

An antibody to p16INK4A recognizes a modified form of galectin-3. p16INK4A抗体识别半乳糖凝集素-3的修饰形式。

Hybridoma Pub Date : 2001-06-01 DOI: 10.1089/027245701750293493

J. Gump, J. Koh

引用次数: 3

Generation and characterization of monoclonal antibodies to the neural crest. 神经嵴单克隆抗体的产生和鉴定。

Hybridoma Pub Date : 2001-06-01 DOI: 10.1089/027245701750293538

T. Shakil, M. Richardson, E. Waldron, Gillian Conde, Sally Wood, Y. Bland, Gary M. Reynolds, Paul G. Murray, Paul N. Nelson

{"title":"Generation and characterization of monoclonal antibodies to the neural crest.","authors":"T. Shakil, M. Richardson, E. Waldron, Gillian Conde, Sally Wood, Y. Bland, Gary M. Reynolds, Paul G. Murray, Paul N. Nelson","doi":"10.1089/027245701750293538","DOIUrl":"https://doi.org/10.1089/027245701750293538","url":null,"abstract":"The generation of monoclonal antibodies (MAbs) specific for quail neural crest may provide valuable tools for studying the differentiation of embryonic precursor cells. Unfortunately, relatively few antibodies are available because of the difficulty in obtaining sufficient cells for in vivo immunization strategies. We have overcome this problem by using intrasplenic immunization with formaldehyde-fixed cells harvested from neural crest cultures. In addition, booster injections of cultured whole-embryo cells were administered intraperitoneally. Following two fusions, a total of 18 hybridomas were generated with antibody reactivity to the cytoplasm of neural crest cells. Furthermore, 32 were reactive against both somite (a noncrest mesodermal control) and crest cultures, whilst 15 were not reactive. Out of those hybridomas reactive with neural crest, six designated 160D, 164D, OE, 12E, 120E and 124E were further characterized. Interestingly MAb supernatants OE, 12E, 120E, and 124E exhibited reactivity against some but not all neural crest cells suggesting that they might recognise subpopulations. Immunoglobulin isotyping of supernatants revealed that 4 (160D, 164D, OE, and 120E) were IgM and 2 (12E and 124E) were IgG(2b). On assessing their reactivity against human tissue sections, all six hybridoma supernatants cross-reacted with neuroendocrine cells within appendix, colon and rectum. These MAbs could provide novel reagents for the understanding of neural crest development.","PeriodicalId":55044,"journal":{"name":"Hybridoma","volume":"20 3 1","pages":"199-203"},"PeriodicalIF":0.0,"publicationDate":"2001-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/027245701750293538","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"60500108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

The development of monoclonal antibody therapy in leukemias. 单克隆抗体治疗白血病的进展。

Hybridoma Pub Date : 2001-06-01 DOI: 10.1089/027245701750293466

K. Syrigos, K. Pliarchopoulou, K. Harrington

{"title":"The development of monoclonal antibody therapy in leukemias.","authors":"K. Syrigos, K. Pliarchopoulou, K. Harrington","doi":"10.1089/027245701750293466","DOIUrl":"https://doi.org/10.1089/027245701750293466","url":null,"abstract":"Conventional cytotoxic management of leukemia has less than optimal results, while it is associated with life-threatening toxic effects due to lack of specificity for hematopoietic cells. Therefore, novel therapeutic strategies with monoclonal antibodies (MAbs) are being explored for delivering chemotherapy or radiation directly to malignant cells. Recently, anti-CD33 antibodies have been engineered to target malignant myeloid and immature normal cells and have been used to deliver cytotoxic agents or radiation to leukemic cells. 131I-labeled anti-CD45 antibodies are used in combination with conventional chemotherapy in leukemic patients receiving marrow transplantation. Additionally, the emergence of Rituximab (against CD20) and Campath-1H (against CD52) for chronic lymphocytic leukemia (CLL) has provided encouraging clinical results for the prognosis of this disease. In conclusion, there has been ongoing research indicating that the approach of patients with leukemia through the application of MAbs might be safer and more effective than current treatment. Considering the preliminary data, MAb therapy appears to be a new, promising weapon in the oncologist's armentarium.","PeriodicalId":55044,"journal":{"name":"Hybridoma","volume":"20 3 1","pages":"145-8"},"PeriodicalIF":0.0,"publicationDate":"2001-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/027245701750293466","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"60499249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Method for rapidly preparing emulsion of antigen solution with adjuvant. 一种快速制备佐剂抗原溶液乳剂的方法。

Hybridoma Pub Date : 2001-06-01 DOI: 10.1089/027245701750293547

D. Wang

引用次数: 0