Yuanyuan Li, Jinze Mu, Qingliang Li, Huabing Liu, Xuefeng Yuan, Deya Wang
{"title":"Analysis of Subcellular Localization and Pathogenicity of Plum Bark Necrosis Stem-Pitting Associated Virus Protein P6","authors":"Yuanyuan Li, Jinze Mu, Qingliang Li, Huabing Liu, Xuefeng Yuan, Deya Wang","doi":"10.32604/phyton.2023.028237","DOIUrl":"https://doi.org/10.32604/phyton.2023.028237","url":null,"abstract":"Infection of plum bark necrosis stem pitting associated virus (PBNSPaV) has been reported in many prunus species in several countries, causing signi�cant economic losses. The very small proteins encoded by plant viruses are often overlooked due to their short sequences and uncertain signi�cance. However, numerous studies have indicated that they might play important roles in the pathogenesis of virus infection. The role of small hydrophobic protein P6, encoded by the open reading frame 2 of PBNSPaV, has not been well explored. In this study, we ampli�ed the P6 fragment from a PBNSPaV isolate by RT-PCR using speci�c primers and found that it is 174 bp long and encodes a protein of approximately 6.3 kD with a transmembrane domain. Subcellular localization analysis of P6 proteins in tobacco leaves showed that P6 localizes in cytomembrane and nuclear membrane. To further clarify the pathogenicity of P6 proteins, a PVX-P6 expression vector was constructed by inserting the p6 fragment into a potato virus X (PVX)-based vector and transformed into Agrobacterium tumefaciens GV3101. In�ltration of N. benthamiana with the PVX vector-transformed A. tumefaciens led to slight mosaic symptoms at 14 days of post-inoculation. Meanwhile, in�ltration with the PVX-P6 vector-transformed A. tumefaciens resulted in no signi�cant symptoms. These results demonstrated that heterologous expression of P6 in N. benthamiana could not enhance the pathogenicity of PVX. Our study indicates that p6 may not be a potential pathogenic factor associate with the causing of symptoms, and mode of action of PBNSPaV-P6 protein remain to be further studied.","PeriodicalId":54605,"journal":{"name":"Phyton-Annales Rei Botanicae","volume":"1 1","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69898148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Kausar, N. Zahra, Humaira Kiran, S. Asim, A. Raza, A. Raza
{"title":"The Ability of Some Aquatic and Terrestrial Plants to Purify Domestic Wastewater","authors":"A. Kausar, N. Zahra, Humaira Kiran, S. Asim, A. Raza, A. Raza","doi":"10.32604/phyton.2023.028264","DOIUrl":"https://doi.org/10.32604/phyton.2023.028264","url":null,"abstract":"","PeriodicalId":54605,"journal":{"name":"Phyton-Annales Rei Botanicae","volume":"1 1","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69898290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Sagar, Md. Sabibul Haque, Md. Alamgir Hossain, Md. Nesar Uddin, Jannat E. Tajkia, Md. Ashik Mia, Toufika Hossain Shabi, Md. Solaiman Ali Fakir, M. Abdul Kader, W. Soufan, Md. Atikur Rahman, Muhammad Aamir Iqbal, Mohammad Sohidul Islam, A. El Sabagh, A. K. M. Zakir Hossain
{"title":"Genotypic Divergence, Photosynthetic Efficiency, Sodium Extrusion, and Osmoprotectant Regulation Conferred Salt Tolerance in Sorghum","authors":"A. Sagar, Md. Sabibul Haque, Md. Alamgir Hossain, Md. Nesar Uddin, Jannat E. Tajkia, Md. Ashik Mia, Toufika Hossain Shabi, Md. Solaiman Ali Fakir, M. Abdul Kader, W. Soufan, Md. Atikur Rahman, Muhammad Aamir Iqbal, Mohammad Sohidul Islam, A. El Sabagh, A. K. M. Zakir Hossain","doi":"10.32604/phyton.2023.028974","DOIUrl":"https://doi.org/10.32604/phyton.2023.028974","url":null,"abstract":"","PeriodicalId":54605,"journal":{"name":"Phyton-Annales Rei Botanicae","volume":"1 1","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69898696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Exploring Splicing Variants and Novel Genes in Sacred Lotus Based on RNA-seq Data","authors":"","doi":"10.32604/phyton.2023.029482","DOIUrl":"https://doi.org/10.32604/phyton.2023.029482","url":null,"abstract":"","PeriodicalId":54605,"journal":{"name":"Phyton-Annales Rei Botanicae","volume":"1 1","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69898810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effects of Manganese on the Antioxidant System and Related Gene Expression Levels in the “Hong Yang” Kiwifruit Seedlings","authors":"Chongpei Zheng, Liangliang Li, Zhencheng Han, Weijie Li, X. Wen","doi":"10.32604/phyton.2023.029891","DOIUrl":"https://doi.org/10.32604/phyton.2023.029891","url":null,"abstract":"","PeriodicalId":54605,"journal":{"name":"Phyton-Annales Rei Botanicae","volume":"1 1","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69899124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jing Yang, Tianxiunan Pu, Ke Wan, Linqi Wang, Yuanshuai Shi, Xu Luo, Jie Tan, Dongmei Wang, Yang Liu
{"title":"Transcriptome and Metabolome Revealed the Mechanism of NtBRL3 Overexpression Tobacco (Nicotiana tabacum L. K326) in Response to Drought Stress","authors":"Jing Yang, Tianxiunan Pu, Ke Wan, Linqi Wang, Yuanshuai Shi, Xu Luo, Jie Tan, Dongmei Wang, Yang Liu","doi":"10.32604/phyton.2023.030301","DOIUrl":"https://doi.org/10.32604/phyton.2023.030301","url":null,"abstract":"","PeriodicalId":54605,"journal":{"name":"Phyton-Annales Rei Botanicae","volume":"1 1","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69899364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hosny Abdel Aziz, M. Sharaf, Magdy A Omar, A. Abou El-Yazied, Nada Ibrahim AlJwaizea, Shaimaa Ismail, Mohamed M. A. Omar, K. Alharbi, Amr A. Elkelish, Moataz M. Tawfik
{"title":"Improvement of Selected Morphological, Physiological, and Biochemical Parameters of Banana (Musa acuminata L.) Using Potassium Silicate under Drought Stress Condition Grown in vitro","authors":"Hosny Abdel Aziz, M. Sharaf, Magdy A Omar, A. Abou El-Yazied, Nada Ibrahim AlJwaizea, Shaimaa Ismail, Mohamed M. A. Omar, K. Alharbi, Amr A. Elkelish, Moataz M. Tawfik","doi":"10.32604/phyton.2023.026769","DOIUrl":"https://doi.org/10.32604/phyton.2023.026769","url":null,"abstract":"","PeriodicalId":54605,"journal":{"name":"Phyton-Annales Rei Botanicae","volume":"1 1","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69894523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Luli Li, Nigel G. Halford, Huihui Wang, Yingjie Zong, Zhenzhu Guo, R. Lu, Chenghong Liu, Zhiwei Chen
{"title":"Genetic Diversity and Population Structure Analysis of Barley Landraces from Shanghai Region Using Genotyping-by-Sequencing","authors":"Luli Li, Nigel G. Halford, Huihui Wang, Yingjie Zong, Zhenzhu Guo, R. Lu, Chenghong Liu, Zhiwei Chen","doi":"10.32604/phyton.2023.026946","DOIUrl":"https://doi.org/10.32604/phyton.2023.026946","url":null,"abstract":"","PeriodicalId":54605,"journal":{"name":"Phyton-Annales Rei Botanicae","volume":"1 1","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69895321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abdullah M. Algahtani, Fahed A. Al-Mana, Khalid M. Elhindi
{"title":"Types of Irrigation Water and Soil Amendment Affect the Growth and Flowering of Petunia x alkinsiana ‘Bravo Pinc’","authors":"Abdullah M. Algahtani, Fahed A. Al-Mana, Khalid M. Elhindi","doi":"10.32604/phyton.2022.022850","DOIUrl":"https://doi.org/10.32604/phyton.2022.022850","url":null,"abstract":"","PeriodicalId":54605,"journal":{"name":"Phyton-Annales Rei Botanicae","volume":"1 1","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69889343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Kesheng Lin, Z. Wang, Li Wang, Jiawen Zhou, Lijuan Han, Jingjing Wen, T. Dong, R. Duan, Ning Li
{"title":"A Rapid Determination of 30 Prohibited Pesticide Residues in Lycii Fructus by UPLC-MS/MS","authors":"Kesheng Lin, Z. Wang, Li Wang, Jiawen Zhou, Lijuan Han, Jingjing Wen, T. Dong, R. Duan, Ning Li","doi":"10.32604/phyton.2022.023763","DOIUrl":"https://doi.org/10.32604/phyton.2022.023763","url":null,"abstract":"","PeriodicalId":54605,"journal":{"name":"Phyton-Annales Rei Botanicae","volume":"1 1","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69889725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}