Toxicology in Vitro最新文献_第5页

The pesticide fungicide difenoconazole modulates the biophysical properties of sodium channel Nav1.5 农药杀菌剂异苯唑调节钠通道Nav1.5的生物物理性质。

IF 2.7 3区医学

Toxicology in Vitro Pub Date : 2026-01-01 Epub Date: 2025-09-24 DOI: 10.1016/j.tiv.2025.106152

V. Fogaça-Santos , F.S. Alcântara , M.R.L. Conceição , L.P. Marques , J.L. Teixeira-Fonseca , D.J.B. Orts , J. Branquinho , R.L. Morais , K.O. Mota , D.S. Souza , J.B. Pesquero , D. Roman-Campos

{"title":"The pesticide fungicide difenoconazole modulates the biophysical properties of sodium channel Nav1.5","authors":"V. Fogaça-Santos , F.S. Alcântara , M.R.L. Conceição , L.P. Marques , J.L. Teixeira-Fonseca , D.J.B. Orts , J. Branquinho , R.L. Morais , K.O. Mota , D.S. Souza , J.B. Pesquero , D. Roman-Campos","doi":"10.1016/j.tiv.2025.106152","DOIUrl":"10.1016/j.tiv.2025.106152","url":null,"abstract":"<div><div>Difenoconazole (DIF) is a widely used fungicide in agriculture, but it is unknown if it can modulate the excitability of the heart tissue. Thus, we investigated the acute effect of DIF on the electrical properties of heart tissue, with a particular focus on the cardiac sodium channel (Na<sub>v</sub>1.5) using transient expression of human Na<sub>v</sub>1.5 in human kidney cell (HEK) and human cardiomyocytes derived from induced pluripotent stem cells (CM-hiPSCs). DIF reduced the peak amplitude of sodium current (I<sub>Na</sub>) in a concentration dependent manner. While DIF did not alter the voltage dependence of activation, it shifted the I<sub>Na</sub> inactivation curve to more negative potentials and delayed the recovery from inactivation. <em>In silico</em> simulation identified aminoacids Thr1710 and Val1765 as critical for the interaction between DIF with Na<sub>v</sub>1.5. DIF partially blocked the late sodium current induced by deltamethrin, a pyrethroid pesticide, suggesting a potential interaction between these compounds in the environment. In CM-hiPSCs, DIF completely abolished the action potential. These findings suggest that DIF may modulate the biophysical properties of Na<sub>v</sub>1.5 and modulate cellular excitability which may cause reduced heart excitability, an DIF can also counteracting the effects of pyrethroid pesticides, but further studies using DIF concentrations closer to human exposure levels are necessary.</div></div>","PeriodicalId":54423,"journal":{"name":"Toxicology in Vitro","volume":"110 ","pages":"Article 106152"},"PeriodicalIF":2.7,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145180280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Use of resazurin-based metabolic assays as a continuous measure of viability in three-dimensional (3D) in vitro cell culture: A study with Presto Blue™ 使用瑞沙脲为基础的代谢测定作为三维（3D）体外细胞培养活力的连续测量：Presto Blue™的研究。

IF 2.7 3区医学

Toxicology in Vitro Pub Date : 2026-01-01 Epub Date: 2025-09-16 DOI: 10.1016/j.tiv.2025.106127

Veronica M. Lucian, Robert C. Carlisle, Constantin C. Coussios, Malavika Nair

{"title":"Use of resazurin-based metabolic assays as a continuous measure of viability in three-dimensional (3D) in vitro cell culture: A study with Presto Blue™","authors":"Veronica M. Lucian, Robert C. Carlisle, Constantin C. Coussios, Malavika Nair","doi":"10.1016/j.tiv.2025.106127","DOIUrl":"10.1016/j.tiv.2025.106127","url":null,"abstract":"<div><div>Testing cell viability in three-dimensional (3D) <em>in vitro</em> cell cultures is not always straightforward. Reduction-based metabolic assays have been suggested as a rapid, reportedly non-toxic mechanism for monitoring cell viability in continuous culture experiments. However, in 3D cultures these assays have been predominantly used semiquantitatively to indicate relative trends in viability. Obtaining quantitative values of cell number is often challenging, particularly when performing continuous measurements of viability when compared with end-point assessments. This work presents experiments performed to robustly identify and address the sources of inconsistency in existing Presto Blue™ methods for 3D cultures. Using human dermal fibroblasts (HDFs) as a model cell line seeded onto type I collagen scaffolds, we examine the impact of Presto Blue™ assays on reported cell numbers in 2D versus 3D cultures. We separate the impact of potential dye-induced toxicity from manipulation-induced cell detachment by considering the effects of passage number, incubation period, modifications of substrate chemistry and duration of total dye exposure as independent variables. The work presented includes a finalised protocol that demonstrates that while resazurin-based reduction assays remain a valuable tool for rapid assessment of cell metabolism and/or viability, additional considerations of the substrate and sampling frequencies are needed in 3D cultures to ensure accurate and reproducible results.</div></div>","PeriodicalId":54423,"journal":{"name":"Toxicology in Vitro","volume":"110 ","pages":"Article 106127"},"PeriodicalIF":2.7,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145088106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Collaborative evaluation of in silico predictions for high throughput toxicokinetics 高通量毒物动力学的协同评估。

IF 2.7 3区医学

Toxicology in Vitro Pub Date : 2026-01-01 Epub Date: 2025-09-20 DOI: 10.1016/j.tiv.2025.106150

John F. Wambaugh , Nisha S. Sipes , Gilberto Padilla Mercado , Jon A. Arnot , Linda Bertato , Trevor N. Brown , Nicola Chirico , Christopher Cook , Daniel E. Dawson , Sarah E. Davidson-Fritz , Stephen S. Ferguson , Michael-Rock Goldsmith , Chris M. Grulke , Richard S. Judson , Kamel Mansouri , Grace Patlewicz , Ester Papa , Prachi Pradeep , Alessandro Sangion , Risa R. Sayre , Michael J. Devito

{"title":"Collaborative evaluation of in silico predictions for high throughput toxicokinetics","authors":"John F. Wambaugh , Nisha S. Sipes , Gilberto Padilla Mercado , Jon A. Arnot , Linda Bertato , Trevor N. Brown , Nicola Chirico , Christopher Cook , Daniel E. Dawson , Sarah E. Davidson-Fritz , Stephen S. Ferguson , Michael-Rock Goldsmith , Chris M. Grulke , Richard S. Judson , Kamel Mansouri , Grace Patlewicz , Ester Papa , Prachi Pradeep , Alessandro Sangion , Risa R. Sayre , Michael J. Devito","doi":"10.1016/j.tiv.2025.106150","DOIUrl":"10.1016/j.tiv.2025.106150","url":null,"abstract":"<div><div>High throughput toxicokinetic (HTTK) methods address chemical risk assessment data gaps but require chemical-specific values that can be obtained by <em>in vitro</em> measurements or <em>in silico</em> models. In this study, seven quantitative structure property relationship (QSPR) models were used to estimate intrinsic hepatic clearance (Cl<sub>int</sub>), fraction of chemical unbound in plasma (f<sub>up</sub>), and/or TK elimination half-life (t<sub>½</sub>). Performance of the QSPR models was evaluated using literature time-course <em>in vivo</em> TK data, mainly from rats. Simulations of the <em>in vivo</em> data were made with a high throughput physiologically based TK (HT-PBTK) model using the different QSPR model predictions as inputs. We estimate that using rat <em>in vivo</em> data to evaluate QSPR models trained on human <em>in vitro</em> measured data might inflate error estimates by as much as root mean squared log<sub>10</sub> error (RMSLE) 0.8. A sensitivity analysis showed that Cl<sub>int</sub> and f<sub>up</sub> parameters inform predictions of area under the curve (AUC) and steady-state concentration (C<sub>ss</sub>). We estimate that AUC can be predicted by HTTK with RMSLE 0.9 using <em>in vitro</em> measurements and 0.6–0.8 using QSPR model values. We anticipate that, for some novel compounds, QSPRs for HTTK input parameters will give predictions of TK similar to those based on <em>in vitro</em> measurements.</div></div>","PeriodicalId":54423,"journal":{"name":"Toxicology in Vitro","volume":"110 ","pages":"Article 106150"},"PeriodicalIF":2.7,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145126470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hyperuricemia impairs endothelial function through SMS2-dependent activation of the endoplasmic reticulum stress response 高尿酸血症通过sms2依赖性的内质网应激反应激活损害内皮功能。

IF 2.7 3区医学

Toxicology in Vitro Pub Date : 2026-01-01 Epub Date: 2025-08-25 DOI: 10.1016/j.tiv.2025.106133

Liyuan Wang, Ruifang Zhang, Xuelian Liu

{"title":"Hyperuricemia impairs endothelial function through SMS2-dependent activation of the endoplasmic reticulum stress response","authors":"Liyuan Wang, Ruifang Zhang, Xuelian Liu","doi":"10.1016/j.tiv.2025.106133","DOIUrl":"10.1016/j.tiv.2025.106133","url":null,"abstract":"<div><div>Hyperuricemia is a recognized risk factor for cardiovascular diseases, including peripheral arterial disease (PAD), though molecular mechanisms linking uric acid to endothelial dysfunction remain unclear. This study investigates the role of sphingomyelin synthase 2 (SMS2) and endoplasmic reticulum (ER) stress in uric acid-induced endothelial impairment. Human umbilical vein endothelial cells were exposed to physiologically relevant concentrations of uric acid, with SMS2 function modulated through siRNA knockdown and ER stress inhibited using 4-phenylbutyric acid. Uric acid exhibited concentration-dependent cytotoxicity (IC50 ∼ 9 mg/dL) and significantly upregulated SMS2 expressions. At this concentration, uric acid significantly increased apoptosis, impaired migratory capacity, and diminished angiogenic potential (<em>p</em> < 0.01). These functional deficits coincided with marked elevation of ER stress markers and intracellular calcium disruption. Notably, both SMS2 knockdown and ER stress inhibition substantially reversed these uric acid-induced endothelial dysfunctions, restoring cell survival, migration, and angiogenic capacity while normalizing ER stress markers and calcium homeostasis (<em>p</em> < 0.01). These findings identify SMS2 as a potential therapeutic target for vascular complications in hyperuricemia and suggest ER stress modulators may protect against uric acid-induced endothelial damage.</div></div>","PeriodicalId":54423,"journal":{"name":"Toxicology in Vitro","volume":"110 ","pages":"Article 106133"},"PeriodicalIF":2.7,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144979070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

NAM-based development of a predictive test model for evaluating skin mildness potential of rinse-off products via integrated in vitro assays 基于nama的预测测试模型的开发，通过综合体外分析来评估冲洗产品的皮肤温和性潜力。

IF 2.7 3区医学

Toxicology in Vitro Pub Date : 2026-01-01 Epub Date: 2025-08-29 DOI: 10.1016/j.tiv.2025.106134

Yi-Peng Ng , Mei-Leng Tee , Shi-Ngar Pang, Chung-Lee Yee, Ursula Rho-Wan Chong, Khan-Loon Ng, Ka-Heng Lee, Mong-Sah Toh

{"title":"NAM-based development of a predictive test model for evaluating skin mildness potential of rinse-off products via integrated in vitro assays","authors":"Yi-Peng Ng , Mei-Leng Tee , Shi-Ngar Pang, Chung-Lee Yee, Ursula Rho-Wan Chong, Khan-Loon Ng, Ka-Heng Lee, Mong-Sah Toh","doi":"10.1016/j.tiv.2025.106134","DOIUrl":"10.1016/j.tiv.2025.106134","url":null,"abstract":"<div><div>The Forearm Controlled Application Test (FCAT) is widely used to assess skin irritancy and drying potential of rinse-off product but limited by its low throughput and long testing duration. To address these limitations, we explored the development of predictive models using New Approach Methodology (NAM) as a screening tool for evaluating skin mildness potential of rinse-off products. The skin irritancy and drying potential of standard surfactants were evaluated through multiple <em>in vitro</em> assays to identify relevant biomarkers, including a skin irritation bioassay by using Human Reconstituted Skin, a protein solubilization assay by using Zein test and a lipid solubilization assay by using Fatty Acid Methyl Ester (FAME) test. The <em>in vitro</em> test results were correlated to the clinical FCAT data, leading to the development of two multiple linear regression models in predicting changes of Transepidermal Water Loss (TEWL) and skin moisture parameters with R<sup>2</sup> of 0.7062 and 0.8270, respectively. The models achieved prediction accuracies of 100 % and 89 % with relatively low Mean Absolute Error (MAE), outperforming single biomarker model. In summary, this proof-of-concept study demonstrated potential of integrative predictive models to predict clinical outcomes of FCAT, serving as a valuable NAM tool for evaluating the safety and skin mildness potential of rinse-off formulation.</div></div>","PeriodicalId":54423,"journal":{"name":"Toxicology in Vitro","volume":"110 ","pages":"Article 106134"},"PeriodicalIF":2.7,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144979084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Excess curcumin causes cytotoxicity of its nanoconjugate with nanoceria 过量的姜黄素引起其纳米结合物与纳米粒的细胞毒性

IF 2.7 3区医学

Toxicology in Vitro Pub Date : 2026-01-01 Epub Date: 2025-09-03 DOI: 10.1016/j.tiv.2025.106144

Nikita N. Chukavin , Anton L. Popov , Victoria A. Anikina , Daria A. Vinnik , Boris A. Bokl , Nelli R. Popova

{"title":"Excess curcumin causes cytotoxicity of its nanoconjugate with nanoceria","authors":"Nikita N. Chukavin , Anton L. Popov , Victoria A. Anikina , Daria A. Vinnik , Boris A. Bokl , Nelli R. Popova","doi":"10.1016/j.tiv.2025.106144","DOIUrl":"10.1016/j.tiv.2025.106144","url":null,"abstract":"<div><div>Curcumin is a natural bioactive substance with promising biomedical applications. However, the low solubility and stability of curcumin significantly limit its potential use. The development of nanoformulations of curcumin makes it possible to circumvent the above limitations. Cerium dioxide (CeO<sub>2</sub>, NDC) nanoparticles are a promising platform for curcumin binding. They are able to absorb curcumin on their surface, providing increased bioavailability and bioaccumulation. Moreover, NDC have unique enzyme-like properties that can be used for targeted delivery and controlled release of curcumin. Meanwhile, the potential cytotoxicity of such nanoformulations remains poorly understood. In this work, we synthesized the NDC-curcumin nanoconjugate and investigated the effect of excess curcumin on the cytotoxicity of this nanoformulation. Curcumin has been shown to bind to the surface of nanoparticles, forming a colloidally stable nanoconjugate. At the same time, excess curcumin formed a separate nanoscale fraction, which caused the statistically significant cytotoxicity of the nanoconjugate in relation to human keratinocytes (HaCaT), mesenchymal stem cells (hMSc) and fibroblasts (HF). These cell lines represent the cells that form skin, mucous membranes, and connective tissue, with which curcumin nanoformulations can interact through various methods of administration. IC<sub>50</sub> values of curcumin and NDC-curcumin were 400 and 482 μM for HaCaT, 193 and 211 μM for hMSc, 266 and 304 μM for HF, respectively, after 72 h of coincubation. Consequently, NDC provided an increased biocompatibility of curcumin. Application of a rational design of curcumin nanoformulations can help to overcome possible limitations of its practical use due to its toxicity, enhancing its bioactivity.</div></div>","PeriodicalId":54423,"journal":{"name":"Toxicology in Vitro","volume":"110 ","pages":"Article 106144"},"PeriodicalIF":2.7,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145004682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Toxicity assessment of 6PPD-quinone in human lung cells: Insights from BEAS-2B and A549 cell lines 6ppd -醌对人肺细胞的毒性评价：来自BEAS-2B和A549细胞系的见解。

IF 2.7 3区医学

Toxicology in Vitro Pub Date : 2026-01-01 Epub Date: 2025-09-17 DOI: 10.1016/j.tiv.2025.106147

Saja Damdam , Blake Hunnie , Parker Jones , Markus Hecker , Markus Brinkmann , Francisco C. da Silva Junior

{"title":"Toxicity assessment of 6PPD-quinone in human lung cells: Insights from BEAS-2B and A549 cell lines","authors":"Saja Damdam , Blake Hunnie , Parker Jones , Markus Hecker , Markus Brinkmann , Francisco C. da Silva Junior","doi":"10.1016/j.tiv.2025.106147","DOIUrl":"10.1016/j.tiv.2025.106147","url":null,"abstract":"<div><div>N-(1,3-Dimethylbutyl)-<em>N</em>′-phenyl-<em>p</em>-phenylenediamine-quinone (6PPD-Q), an environmental transformation product of the rubber tire antioxidant 6PPD, is increasingly detected in urban environments, yet its impact on human health. This study investigated toxicological effects of 6PPD-Q in two human lung cells: BEAS-2B (a transformed bronchial epithelial cell) and A549 (a lung adenocarcinoma cell). Cells were exposed to 6PPD-Q at concentrations of 5 to 80 ng/mL and effects on cell viability, oxidative stress by generation of reactive oxygen species (ROS), disruption of antioxidant defense response, oxidative DNA damage as determined by formation of 8-hydroxy-2′-deoxyguanosine (8-OHdG), and biotransformation 6PPD-Q were determined. Results showed a biphasic response with an increase in cell viability at lower concentrations, suggesting an adaptive cellular response. ROS production and levels of 8-OHdG significantly increased, indicating an imbalance in redox status and induction of oxidative DNA damage. Two phase I hydroxylation products, 6PPD-Q-4-OH (TPOH1) and 6PPD-Q-phenyl-OH (TPOH2), were identified. In A549 cells, TPOH1 was only in the media, while TPOH2 was found both inside cells and in the media. Moreover, mRNA expression analysis revealed upregulation of metabolism- and oxidative stress-related genes and inflammatory cytokines. These findings suggest that 6PPD-Q may trigger oxidative stress and inflammation in lung cells. This study highlights the effects of 6PPD-Q on lung cells and underscores the need for further research on its long-term impact on human respiratory health.</div></div>","PeriodicalId":54423,"journal":{"name":"Toxicology in Vitro","volume":"110 ","pages":"Article 106147"},"PeriodicalIF":2.7,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145092993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

First toxicity profile of aminoindane-based new psychoactive substances: 2-aminoindane (2-AI, CAS: 2975-41-9) and N-Methyl-2-aminoindane (NM-2-AI, CAS: 24445–44–1) – comprehensive prediction of toxicological endpoints important from clinical and forensic perspective using in silico multi- approach 基于氨基吲哚胺的新型精神活性物质：2-氨基吲哚胺（2-AI, CAS: 2975-41-9）和n -甲基-2-氨基吲哚胺（NM-2-AI, CAS: 24445-44-1）的第一个毒性谱-从临床和法医角度使用硅多方法综合预测毒理学终点很重要。

IF 2.7 3区医学

Toxicology in Vitro Pub Date : 2026-01-01 Epub Date: 2025-09-24 DOI: 10.1016/j.tiv.2025.106149

Kamil Jurowski , Adrian Frydrych

{"title":"First toxicity profile of aminoindane-based new psychoactive substances: 2-aminoindane (2-AI, CAS: 2975-41-9) and N-Methyl-2-aminoindane (NM-2-AI, CAS: 24445–44–1) – comprehensive prediction of toxicological endpoints important from clinical and forensic perspective using in silico multi- approach","authors":"Kamil Jurowski , Adrian Frydrych","doi":"10.1016/j.tiv.2025.106149","DOIUrl":"10.1016/j.tiv.2025.106149","url":null,"abstract":"<div><div>This study provides the first toxicity profile of two aminoindane-based new psychoactive substances (NPS): 2-aminoindane (2-AI) and <em>N</em>-methyl-2-aminoindane (NM-2-AI), using a multi-method <em>in silico</em> approach. Tools including Percepta, ProTox 3.0, ADMETlab 3.0, VEGA QSAR, TEST, QSAR Toolbox, and STopTox were applied to evaluate acute toxicity, systemic organ effects, genotoxicity, skin and eye irritation, and cardiotoxicity. Both compounds were predicted to be acutely toxic <em>via</em> oral, dermal, and inhalation routes. Quantitative LD₅₀ values (rat, oral) ranged from 150 to 560 mg/kg (Percepta), 259.7–112.6 mg/kg (VEGA), and 326–360 mg/kg (ProTox 3.0). Health effect predictions indicated high probabilities of cardiovascular toxicity (2-AI: 80 %, NM-2-AI: 82 %) and pulmonary involvement (66 % and 65 %, respectively). NM-2-AI showed a higher likelihood of genotoxicity (Ames test) with a probability of 66.3 % (ADMETlab), compared to 2-AI (58.7 %). Both substances were predicted to be non-irritant to the eye, but skin irritation was probable for 2-AI (80 %, StopTox) and less so for NM-2-AI (60 %). hERG inhibition risk was low (IC₅₀: 391.7 μM for 2-AI; 126.9 μM for NM-2-AI). The <em>in silico</em> multi-tool strategy yielded convergent and interpretable data, supporting early toxicological characterization. These results are relevant for clinical management of NPS intoxications and for forensic interpretation in the absence of experimental data.</div></div>","PeriodicalId":54423,"journal":{"name":"Toxicology in Vitro","volume":"110 ","pages":"Article 106149"},"PeriodicalIF":2.7,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145180335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cytotoxic effects of a LED/UV nail polish dryer in human keratinocytes LED/UV指甲油干燥器对人角质形成细胞的细胞毒性作用。

IF 2.7 3区医学

Toxicology in Vitro Pub Date : 2025-12-01 Epub Date: 2025-07-29 DOI: 10.1016/j.tiv.2025.106124

Alexandra Lopes, Patrícia Pereira, Helena Oliveira

{"title":"Cytotoxic effects of a LED/UV nail polish dryer in human keratinocytes","authors":"Alexandra Lopes, Patrícia Pereira, Helena Oliveira","doi":"10.1016/j.tiv.2025.106124","DOIUrl":"10.1016/j.tiv.2025.106124","url":null,"abstract":"<div><div>Nail polish dryers are radiation-emitting devices designed to dry and cure nail polishes. These devices primarily emit radiation within the ultraviolet A (UVA) range, with some also emitting visible light, particularly those equipped with light-emitting diodes (LEDs). The increasing use of nail polish dryers, combined with scientific concerns regarding the potential effects of UVA radiation on human skin, has raised significant safety questions. Recent studies have demonstrated cytotoxic, genotoxic and mutagenic effects in both human and murine cells. When these findings are considered alongside earlier reports linking the use of these devices to skin cancer, there is a clear need for a broader evaluation of their potential health impacts. This study aimed to evaluate the effects of realistic radiation exposures, when considering the actual gel-nail application process, on cell viability and intracellular reactive oxygen species (ROS) levels, in human keratinocytes. Cytotoxic effects were confirmed, with cell mortality rates exceeding 95 % and intracellular ROS levels increasing by over 250 % (72 h post-exposure), depending on the positioning of the cell culture within the device. These results underscore the need for a more thorough characterization of the cytotoxic effects caused by the radiation emitted by nail polish dryers.</div></div>","PeriodicalId":54423,"journal":{"name":"Toxicology in Vitro","volume":"109 ","pages":"Article 106124"},"PeriodicalIF":2.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144762358","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of gadolinium oxide based nanoparticles on genotoxic, cytotoxic and oxidative stress in human lymphocytes 氧化钆纳米颗粒对人淋巴细胞基因毒性、细胞毒性和氧化应激的影响。

IF 2.6 3区医学

Toxicology in Vitro Pub Date : 2025-12-01 Epub Date: 2025-07-13 DOI: 10.1016/j.tiv.2025.106106

Isela Álvarez-González , Lizbeth Espinosa-García , Felipe de Jesús Carrillo-Romo , Antonieta García-Murillo , José A. Morales-González , Eduardo O. Madrigal-Santillán , Eduardo Madrigal-Bujaidar

{"title":"Impact of gadolinium oxide based nanoparticles on genotoxic, cytotoxic and oxidative stress in human lymphocytes","authors":"Isela Álvarez-González , Lizbeth Espinosa-García , Felipe de Jesús Carrillo-Romo , Antonieta García-Murillo , José A. Morales-González , Eduardo O. Madrigal-Santillán , Eduardo Madrigal-Bujaidar","doi":"10.1016/j.tiv.2025.106106","DOIUrl":"10.1016/j.tiv.2025.106106","url":null,"abstract":"<div><div>Gadolinium-based nanoparticles are presently applied in biomedicine and industry, and their potential is increasing. In this report we synthetized three nanoparticles by the sol-gel, supercritical drying method: Gd<sub>2</sub>O<sub>3</sub>, Gd<sub>2</sub>O<sub>3</sub> doped with Eu<sup>+3</sup>, and Gd<sub>2</sub>O<sub>3</sub> doped with Eu<sup>+3</sup>and functionalized with thenoyltrifluoroacetone (TTA). The nanoparticles were characterized for luminescence, morphology, size, and Z potential. The results showed that the highest luminescence was reached with the TTA functionalized nanoparticle, all of them had a porous net structure that decreased to 21 nm in the last nanoparticle, and the Z potential was found from −2 to −12 mV. To determine their geno/cytotoxic potential we performed the cytochalasine-block micronucleus cytome, and the MTT assays in human lymphocytes. With these assays, we demonstrated that the two main genotoxic effects were the nuclear buds and nucleoplasmic bridges, and that nanoparticles decreased the cellular proliferation, mainly because of the induction of apoptosis (about 70 %) in contrast with the 30 % of necrosis. Finally, a significant lipid and protein oxidation increase was determined in the nanoparticles. Our results suggest that the observed geno/cytotoxic damage may be related with the presence of oxidative stress.</div></div>","PeriodicalId":54423,"journal":{"name":"Toxicology in Vitro","volume":"109 ","pages":"Article 106106"},"PeriodicalIF":2.6,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144644152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0