High-Throughput最新文献_第6页

Molecular Lesions of Insulator CTCF and Its Paralogue CTCFL (BORIS) in Cancer: An Analysis from Published Genomic Studies. 绝缘子CTCF及其旁系CTCFL (BORIS)在癌症中的分子病变:来自已发表的基因组研究的分析。

High-Throughput Pub Date : 2018-10-01 DOI: 10.3390/ht7040030

Ioannis A Voutsadakis

引用次数: 9

Current High-Throughput Approaches of Screening Modulatory Effects of Xenobiotics on Cytochrome P450 (CYP) Enzymes. 目前筛选外源药物对细胞色素P450 (CYP)酶调节作用的高通量方法

High-Throughput Pub Date : 2018-09-29 DOI: 10.3390/ht7040029

Yee Tze Ung, Chin Eng Ong, Yan Pan

{"title":"Current High-Throughput Approaches of Screening Modulatory Effects of Xenobiotics on Cytochrome P450 (CYP) Enzymes.","authors":"Yee Tze Ung, Chin Eng Ong, Yan Pan","doi":"10.3390/ht7040029","DOIUrl":"https://doi.org/10.3390/ht7040029","url":null,"abstract":"Cytochrome P450 (CYP) is a critical drug-metabolizing enzyme superfamily. Modulation of CYP enzyme activities has the potential to cause drug⁻drug/herb interactions. Drug⁻drug/herb interactions can lead to serious adverse drug reactions (ADRs) or drug failures. Therefore, there is a need to examine the modulatory effects of new drug entities or herbal preparations on a wide range of CYP isoforms. The classic method of quantifying CYP enzyme activities is based on high-performance liquid chromatography (HPLC), which is time- and reagent-consuming. In the past two decades, high-throughput screening methods including fluorescence-based, luminescence-based, and mass-spectrometry-based assays have been developed and widely applied to estimate CYP enzyme activities. In general, these methods are faster and use lower volume of reagents than HPLC. However, each high-throughput method has its own limitations. Investigators may make a selection of these methods based on the available equipment in the laboratory, budget, and enzyme sources supplied. Furthermore, the current high-throughput systems should look into developing a reliable automation mechanism to accomplish ultra-high-throughput screening in the near future.","PeriodicalId":53433,"journal":{"name":"High-Throughput","volume":"7 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3390/ht7040029","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36541024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 24

The Cytoscan HD Array in the Diagnosis of Neurodevelopmental Disorders. 细胞扫描HD阵列在神经发育障碍诊断中的应用。

High-Throughput Pub Date : 2018-09-14 DOI: 10.3390/ht7030028

Francesca Scionti, Maria Teresa Di Martino, Licia Pensabene, Valentina Bruni, Daniela Concolino

引用次数: 17

Transcriptional Identification of Related Proteins in the Immune System of the Crayfish Procambarus clarkii. 克氏原螯虾免疫系统中相关蛋白质的转录鉴定

High-Throughput Pub Date : 2018-09-12 DOI: 10.3390/ht7030026

Gabina Calderón-Rosete, Juan Antonio González-Barrios, Manuel Lara-Lozano, Celia Piña-Leyva, Leonardo Rodríguez-Sosa

{"title":"Transcriptional Identification of Related Proteins in the Immune System of the Crayfish Procambarus clarkii.","authors":"Gabina Calderón-Rosete, Juan Antonio González-Barrios, Manuel Lara-Lozano, Celia Piña-Leyva, Leonardo Rodríguez-Sosa","doi":"10.3390/ht7030026","DOIUrl":"10.3390/ht7030026","url":null,"abstract":"The freshwater crayfish Procambarus clarkii is an animal model employed for physiological and immunological studies and is also of great economic importance in aquaculture. Although it is a species of easy husbandry, a high percentage of its production is lost annually as a result of infectious diseases. Currently, genetic information about the immune system of crustaceans is limited. Therefore, we used the abdominal nerve cord from P. clarkii to obtain its transcriptome using Next Generation Sequencing (NGS) to identify proteins that participate in the immune system. The reads were assembled de novo and consensus sequences with more than 3000 nucleotides were selected for analysis. The transcripts of the sequences of RNA were edited for annotation and sent to the GenBank database of the National Center for Biotechnology Information (NCBI). We made a list of accession numbers of the sequences which were organized by the putative role of the immune system pathway in which they participate. In this work, we report on 80 proteins identified from the transcriptome of crayfish related to the immune system, 74 of them being the first reported for P. clarkii. We hope that the knowledge of these sequences will contribute significantly to the development of future studies of the immune system in crustaceans.","PeriodicalId":53433,"journal":{"name":"High-Throughput","volume":"7 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6165390/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36489882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Obesity Proteomics: An Update on the Strategies and Tools Employed in the Study of Human Obesity. 肥胖蛋白质组学:人类肥胖研究策略和工具的最新进展。

High-Throughput Pub Date : 2018-09-12 DOI: 10.3390/ht7030027

Afshan Masood, Hicham Benabdelkamel, Assim A Alfadda

{"title":"Obesity Proteomics: An Update on the Strategies and Tools Employed in the Study of Human Obesity.","authors":"Afshan Masood, Hicham Benabdelkamel, Assim A Alfadda","doi":"10.3390/ht7030027","DOIUrl":"https://doi.org/10.3390/ht7030027","url":null,"abstract":"Proteomics has become one of the most important disciplines for characterizing cellular protein composition, building functional linkages between protein molecules, and providing insight into the mechanisms of biological processes in a high-throughput manner. Mass spectrometry-based proteomic advances have made it possible to study human diseases, including obesity, through the identification and biochemical characterization of alterations in proteins that are associated with it and its comorbidities. A sizeable number of proteomic studies have used the combination of large-scale separation techniques, such as high-resolution two-dimensional gel electrophoresis or liquid chromatography in combination with mass spectrometry, for high-throughput protein identification. These studies have applied proteomics to comprehensive biochemical profiling and comparison studies while using different tissues and biological fluids from patients to demonstrate the physiological or pathological adaptations within their proteomes. Further investigations into these proteome-wide alterations will enable us to not only understand the disease pathophysiology, but also to determine signature proteins that can serve as biomarkers for obesity and related diseases. This review examines the different proteomic techniques used to study human obesity and discusses its successful applications along with its technical limitations.","PeriodicalId":53433,"journal":{"name":"High-Throughput","volume":"7 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3390/ht7030027","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36487305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

Molecular Characterization of Histamine-Producing Psychrotrophic Bacteria Isolated from Red Octopus (Octopus maya) in Refrigerated Storage. 从红章鱼（Octopus maya）中分离的产组胺嗜冷菌的冷藏分子特征。

High-Throughput Pub Date : 2018-09-04 DOI: 10.3390/ht7030025

Mariel Gullian Klanian, Mariana Delgadillo Díaz, Maria José Sánchez Solís

{"title":"Molecular Characterization of Histamine-Producing Psychrotrophic Bacteria Isolated from Red Octopus (Octopus maya) in Refrigerated Storage.","authors":"Mariel Gullian Klanian, Mariana Delgadillo Díaz, Maria José Sánchez Solís","doi":"10.3390/ht7030025","DOIUrl":"10.3390/ht7030025","url":null,"abstract":"The present study aimed at determining the histamine production capacity of Gram (+) and Gram (-) bacteria isolated from Octopus maya, along with identifying the presence of amino acid decarboxylase genes. Of the total 80 psychrotrophic microorganisms, 32 strains were identified as histamine-forming bacteria. The recombinant DNA technique was used for genotypic identification of histidine (hdc), ornithine (odc), and lysine decarboxylases (ldc) genes. Thirty-two strains were able to produce 60⁻100 ppm in trypticase soy broth with 1.0% l-histidine after 6 h at 20 °C. NR6B showed 98% homology with Hafnia alvei. NR73 represented 18.8% of the total isolates and showed 98% homology with Enterobacter xianfengensis and Enterobacter cloacae. NR6A represented 6% of the total isolates, which were identified as Lactococcus sp. The hdc gen from NR6B showed 100% identity with hdc from Morganella morganii; ldc showed 97.7% identity with ldc from Citrobacter freundii. The Odc gene was detected only in NR73 and showed 100% identity with Enterobacter sp. All the isolated were identified as weak histamine⁻former. The ingestion of a food containing small amounts of histamine has little effect on humans; however, the formation of biogenic amines is often considered as an indicator of hygienic quality; this emphasizes the importance of improving good management practices and storage.","PeriodicalId":53433,"journal":{"name":"High-Throughput","volume":"7 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3390/ht7030025","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36459703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

p-Value Histograms: Inference and Diagnostics. p值直方图:推断和诊断。

High-Throughput Pub Date : 2018-08-31 DOI: 10.3390/ht7030023

Patrick Breheny, Arnold Stromberg, Joshua Lambert

引用次数: 22

Oral Biofilms: Development, Control, and Analysis. 口腔生物膜:发展、控制和分析。

High-Throughput Pub Date : 2018-08-31 DOI: 10.3390/ht7030024

Daniela Berger, Aviva Rakhamimova, Andrew Pollack, Zvi Loewy

引用次数: 82

New Developments in the Synthesis of EMICORON. EMICORON合成的新进展。

High-Throughput Pub Date : 2018-08-29 DOI: 10.3390/ht7030022

Massimo Pitorri, Marco Franceschin, Ilaria Serafini, Alessandro Ciccòla, Claudio Frezza, Armandodoriano Bianco

引用次数: 1

Conformational Design and Characterisation of a Truncated Diamine Oxidase from Arthrobacter globiformis. 球形节杆菌截断二胺氧化酶的构象设计与表征。

High-Throughput Pub Date : 2018-08-25 DOI: 10.3390/ht7030021

Nur Nadia Razali, Nur Hafizah Hashim, Adam Thean Chor Leow, Abu Bakar Salleh

{"title":"Conformational Design and Characterisation of a Truncated Diamine Oxidase from Arthrobacter globiformis.","authors":"Nur Nadia Razali, Nur Hafizah Hashim, Adam Thean Chor Leow, Abu Bakar Salleh","doi":"10.3390/ht7030021","DOIUrl":"https://doi.org/10.3390/ht7030021","url":null,"abstract":"A functional mini protein can be developed by miniaturising its size. The minimisation technique provides an excellent model system for studying native enzymes, especially in creating an alternative novel biocatalyst. Miniaturised proteins may have enhanced stability, a crucial characteristic for large-scale production and industrial applications. In this study, a huge enzyme molecule, known as diamine oxidase (DAO, comprising 700 amino acids), was selected to undergo the process. By retaining the arrangement of the original functional sites of DAO in the fourth domain, a mini DAO can be designed via homology modelling. After several downsizing processes, a final configuration of 220 amino acids displayed high binding affinity towards histamine, a short-chain substrate that was catalysed by the parental DAO. The configuration also showed enhanced affinity towards a long-chain substrate known as spermidine. The gene for the designed protein was cloned and expressed in pET102/TOPO vector and overexpressed in E. coli BL21 (DE3). The new mini DAO had similar temperature tolerance and versatile substrates specificity characteristics as its parental protein. An active mini-protein with these characteristics is potentially useful for several applications such as detecting biogenic amines in the biological fluids and the environment that may give rise to health issues.","PeriodicalId":53433,"journal":{"name":"High-Throughput","volume":"7 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3390/ht7030021","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36431115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4