MCBS Molecular and Cellular Biomedical Sciences最新文献_第4页

Role of Genetics in Anesthesiology 遗传学在麻醉学中的作用

MCBS Molecular and Cellular Biomedical Sciences Pub Date : 2022-03-01 DOI: 10.21705/mcbs.v6i1.229

Z. Maritska, Rani Iswara, Ignatius Aldo Winardi, Y. Marantika, Irfan Ferdinand Tambunan, Lovina Lovina, Mgs. A. Rifqi Murtadho, Sendy Aditya Nugraha, Cendy Legowo, Victor Pulpa Seda, Awang Budi Saksono

引用次数: 0

Neutrophil Extracellular Traps and Its Correlation with Several Pathological Conditions: Prosperities and Deleterious Implications 中性粒细胞胞外陷阱及其与几种病理条件的相关性:繁荣和有害影响

MCBS Molecular and Cellular Biomedical Sciences Pub Date : 2022-03-01 DOI: 10.21705/mcbs.v6i1.224

Phey Liana, K. Murti, Zen Hafy, I. A. Liberty, Tungki Pratama Umar

{"title":"Neutrophil Extracellular Traps and Its Correlation with Several Pathological Conditions: Prosperities and Deleterious Implications","authors":"Phey Liana, K. Murti, Zen Hafy, I. A. Liberty, Tungki Pratama Umar","doi":"10.21705/mcbs.v6i1.224","DOIUrl":"https://doi.org/10.21705/mcbs.v6i1.224","url":null,"abstract":"Neutrophil extracellular traps (NETs) are immune components found in a variety of pathological states. It has been shown to have either beneficial or harmful implications, depending on how it is controlled and has been particularly observed in three major scenarios: infection, autoimmune disease, and cancer. In this article, we compiled some of the roles of NETs in pathological conditions, as well as the benefits of targeting them for improved patient outcomes. The role of NETs were primarily positive in infectious disease, whether caused by bacteria, virus, or fungal infection. In non-infectious inflammatory scenarios, on the other hand, it's the complete opposite, with the effects being mainly deleterious and even worse than the original disease states. Targeting NETs directly or indirectly may help to prevent complications and improve patient outcomes. A plethora of compounds, including immunomodulators, anti-thrombosis, nicotinamide adenine dinucleotide phosphate (NADPH)/reactive oxygen species (ROS) inhibitors, nuclease, and other compounds, may be used to accomplish the therapeutic goals.","PeriodicalId":53387,"journal":{"name":"MCBS Molecular and Cellular Biomedical Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85788147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Non-Synonymous Mutation Analysis of SARS-CoV-2 ORF3a in Indonesia 印度尼西亚SARS-CoV-2 ORF3a非同义突变分析

MCBS Molecular and Cellular Biomedical Sciences Pub Date : 2022-03-01 DOI: 10.21705/mcbs.v6i1.221

Hartiyowidi Yuliawuri, J. Christian, N. Steven

{"title":"Non-Synonymous Mutation Analysis of SARS-CoV-2 ORF3a in Indonesia","authors":"Hartiyowidi Yuliawuri, J. Christian, N. Steven","doi":"10.21705/mcbs.v6i1.221","DOIUrl":"https://doi.org/10.21705/mcbs.v6i1.221","url":null,"abstract":"Background: The report of mutation sites ORF3a SARS CoV-2 in Indonesia is still limited. Some research showed that mutations in ORF3a protein might alter SARS-CoV-2 pathogenesis. Observation of new variants should be conducted as a risk monitoring framework.Materials and method: We assessed the impact of mutations in ORF3a protein by analyzing 3,751 SARS-CoV-2 DNA sequences from the GISAID database from March 2020 until July 2021. The whole-genome sequences were aligned using Clustal Omega Multiple Sequence Alignment from EMBL-EBI and analyzed using BioEdit version 7.2.5 software. The reference whole genome sequence was taken from the Genbank database with accession number NC045512. We excluded the samples containing N letters due to inaccurate reading. Effect of point mutations on protein structure was analyzed using PredictProtein (https://predictprotein.org) and Protein Variation Effect Analyzer (PROVEAN) v1.1.3. online software.Results: We identified five most frequent non-synonymous mutations in ORF3a protein of SARS-CoV-2 which were Q57H (58.04%), S26L (27.25%), S220I (10.37%), D155H (8.98%), and P104S (5.47%).Conclusion: These mutation data showed the phenomenon of amino acid changes in ORF3a SARS-CoV-2 in Indonesia until July 2021. The implication of this mutation needs to be determined in further studies.Keywords: Indonesia, mutations, non-synonymous, SARS-CoV-2, whole genome ","PeriodicalId":53387,"journal":{"name":"MCBS Molecular and Cellular Biomedical Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80600128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Correlation of Serum Nitric Oxide and Urine Malondialdehyde Levels in Non-Hemodialysis Chronic Kidney Disease Patients 非血液透析慢性肾病患者血清一氧化氮和尿丙二醛水平的相关性

MCBS Molecular and Cellular Biomedical Sciences Pub Date : 2022-03-01 DOI: 10.21705/mcbs.v6i1.226

D. Purwati, Arifa Mustika, L. Hakim, M. Thaha

{"title":"Correlation of Serum Nitric Oxide and Urine Malondialdehyde Levels in Non-Hemodialysis Chronic Kidney Disease Patients","authors":"D. Purwati, Arifa Mustika, L. Hakim, M. Thaha","doi":"10.21705/mcbs.v6i1.226","DOIUrl":"https://doi.org/10.21705/mcbs.v6i1.226","url":null,"abstract":"Background: In 2017, about 1.2 million people died because of Chronic Kidney Disease (CKD). Patients with CKD are known to have increased levels of oxidative stress which leads to decrease in NO production. NO is a highly reactive signaling molecule and a major determinant of vascular homeostasis. Thus, the decreased NO can be a risk factor for the development of atherosclerosis and increased cardiovascular risk. Meanwhile, Malondialdehyde (MDA) is known as excellent biomarker for oxidative stress. This study aims to determine the correlation of serum total nitric oxide (NO) and urine MDA levels in non-hemodialysis CKD patients.Materials and Methods: This study was an observational clinical study with a cross sectional design. Fourty-nine CKD subjects were selected by consecutive sampling. The samples for laboratory tests were collected from urine. MDA concentration was measured using the High-Performance Liquid Chromatography (HPLC) kit. NO concentration was measured with Griess reaction method and Total Nitric Oxide Parameter kit. The data were analyzed using the Statistic Package for Social Science (SPPS) software version 16.Results: The data showed significant negative correlations between MDA with NO (r=-0.294; p=0.041).Conclusion: There was a correlation between serum total NO and urine MDA levels in non-hemodialysis CKD patients.Keywords: chronic kidney disease, malondialdehyde, nitric oxide, non-hemodialysis","PeriodicalId":53387,"journal":{"name":"MCBS Molecular and Cellular Biomedical Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88511571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Innate Immune Response to House Dust Mite Allergens in Allergic Asthma 过敏性哮喘患者对屋尘螨过敏原的先天免疫反应

MCBS Molecular and Cellular Biomedical Sciences Pub Date : 2021-11-01 DOI: 10.21705/mcbs.v5i3.217

Winna Soleha, F. C. Iswanti

{"title":"Innate Immune Response to House Dust Mite Allergens in Allergic Asthma","authors":"Winna Soleha, F. C. Iswanti","doi":"10.21705/mcbs.v5i3.217","DOIUrl":"https://doi.org/10.21705/mcbs.v5i3.217","url":null,"abstract":"Asthma is a major health problem and one of the leading causes of death in the world. The prevalence of asthma in Indonesia is high, with a recurrence >50%. Allergic sensitization in asthma is mainly caused by house dust mite (HDM) allergens, both from the mite’s body and its contaminants (e.g., lipopolysaccharides). HDM allergens stimulate several pathways in the innate immune response based on the HDM allergen groups that sensitize them. The innate immune response to HDM allergen exposure occurs when pattern recognition receptors (PRRs) recognizes the allergen, thereby stimulating respiratory epithelial cells to release cytokines, namely, thymic stromal lymphopoietin (TSLP), interleukin-25 (IL -25), and IL-33. The release of IL-25 and IL-33 activates group 2 innate lymphoid cells (ILC2) to release Th2-type cytokines (i.e., IL-5 and IL-13), resulting in allergic airway inflammation via IgE secretion by B cells, recruitment of eosinophils, and respiratory tract remodeling. Dendritic cells induce an adaptive immune response through Th2 activation in the sensitization and effector phases. Other mediators that contributed to the innate immune response include C-C motif chemokine ligand 20 (CCL-20) and granulocyte-macrophage colony-stimulating factor (GM-CSF). A deeper understanding of the components and mechanisms involved in innate immunity against HDM allergens creates the potential to develop alternative therapeutic targets for allergic asthma treatment.Keywords: house dust mite allergens, innate immunity, allergic asthma, respiratory epithelium, inflammatory cytokines","PeriodicalId":53387,"journal":{"name":"MCBS Molecular and Cellular Biomedical Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88654192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Donor HLA Genotyping using Polymerase Chain Reaction- Sequence Specific Primers (PCR-SSP) as Method for Acquiring Donor Panel in Platelet Refractoriness 利用聚合酶链反应-序列特异性引物(PCR-SSP)进行供体HLA基因分型以获得血小板难耐性供体样本

MCBS Molecular and Cellular Biomedical Sciences Pub Date : 2021-11-01 DOI: 10.21705/mcbs.v5i3.209

T. D. Atmakusuma, A. Rachman, N. K. Ritchie

{"title":"Donor HLA Genotyping using Polymerase Chain Reaction- Sequence Specific Primers (PCR-SSP) as Method for Acquiring Donor Panel in Platelet Refractoriness","authors":"T. D. Atmakusuma, A. Rachman, N. K. Ritchie","doi":"10.21705/mcbs.v5i3.209","DOIUrl":"https://doi.org/10.21705/mcbs.v5i3.209","url":null,"abstract":"Background: Evaluation and identification of HLA antibodies in the recipient’s serum is of utmost importance prior to transplantation and transfusion. HLA typing is a steppingstone in proposing a donor panel. In order to obtain the HLA typing, Polymerase Chain Reaction-Sequence Specific Primers (PCR-SSP) can be performed.Materials and method: This is a preliminary study to determine HLA polymorphism by HLA genotyping in 43 blood donors. DNA from the samples was isolated using commercial kits according to the standard protocol. The DNA then was amplified using PCR-SSP methods and analyzed using the provided set in the kit.Results: This study found that the most frequent HLA-A alleles was HLA-A*24 (41.9%). For HLA-B alleles, the most common was HLA-B*15 (28%). Most frequent HLA-A-B haplotypes was HLA-A*24-B*15 (11.3%). The results from this study concurs with that of previous study. However, some alleles might vary due to difference in study population. Determining HLA-typing is of paramount importance in an ethnically diverse country such as Indonesia. In contrast to homogenous caucassian country, difference in ethnicity might cause platelet refractoriness due to incompatibility. HLA-typing would also guide the diagnostic workup and required treatment strategy for platelet refractoriness.Conclusion: From the HLA typing using PCR-SSP in blood donors in Jakarta, we found that the most frequent alleles were HLA-A*24 and HLA-B*15; and the most frequent haplotypes were HLA-A*24-B*15. This study should be upscaled to include larger population and ethnic groups to obtain complete profile of Indonesian population.Keywords: platelet refractoriness, HLA, donor, PCR-SSP, transfusion medicine","PeriodicalId":53387,"journal":{"name":"MCBS Molecular and Cellular Biomedical Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90589989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Alteration in 11 Beta-Hydroxysteroid Dehydrogenase Type-2 (HSD11B2) Gene as A Potential Candidate Parameter for Early Detection of Intrauterine Growth Restriction (IUGR) Events 11 β -羟基类固醇脱氢酶2型(HSD11B2)基因改变作为宫内生长限制(IUGR)事件早期检测的潜在候选参数

MCBS Molecular and Cellular Biomedical Sciences Pub Date : 2021-11-01 DOI: 10.21705/mcbs.v5i3.214

Louis Fabio Jonathan Jusni, P. Patricia, Brigitte Leonie Rosadi

{"title":"Alteration in 11 Beta-Hydroxysteroid Dehydrogenase Type-2 (HSD11B2) Gene as A Potential Candidate Parameter for Early Detection of Intrauterine Growth Restriction (IUGR) Events","authors":"Louis Fabio Jonathan Jusni, P. Patricia, Brigitte Leonie Rosadi","doi":"10.21705/mcbs.v5i3.214","DOIUrl":"https://doi.org/10.21705/mcbs.v5i3.214","url":null,"abstract":"Intrauterine Growth Restriction (IUGR) incidence in Indonesia ranks in the top 10 of the highest in Asia. It is the main perinatal death cause. IUGR also impairs fetal neurodevelopment, which can affect the development of children until later ages. Lack of 11β-hydroxysteroid dehydrogenase type-2 (11β-HSD2) enzyme is influenced by changes in the coding gene, HSD11B2, one of IUGR's causes. The main diagnostic method of IUGR at this time is by using Doppler ultrasound. However, Doppler ultrasound has several limitations as many cases are not detected. Its clinical predictive value in various women is poor, as Doppler ultrasound is not recommended for use in the first trimester, detection of abnormalities in the second trimester seems to be too late for helpful interventions. The study aim is to present an overview concerning HSD11B2 gene alteration in an non-invasive prenatal testing (NIPT) as a possible diagnostic parameter for early detection in IUGR infants. This literature review is based on selected articles and studies taken from the Pubmed, Proquest, and EBSCO databases. A total of 4 studies reported the tendency for DNA methylation and decreased expression of the HSD11B2 gene in IUGR cases. Changes in the HSD11B2 gene have the potential to become a diagnostic parameter in the early detection of infants with IUGR. Further study and investigation of this possibility are needed.Keywords: intrauterine growth restriction, HSD11B2, early detection, diagnostic, non-invasive prenatal testing","PeriodicalId":53387,"journal":{"name":"MCBS Molecular and Cellular Biomedical Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84813399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Validation of a Liquid Chromatography/Tandem Mass Spectrometry Assay for the Quantification of Plasma Dihydroartemisinin 液相色谱/串联质谱法定量血浆双氢青蒿素的验证

MCBS Molecular and Cellular Biomedical Sciences Pub Date : 2021-11-01 DOI: 10.21705/mcbs.v5i3.194

Dona Arlinda, Intan Sari Oktoberia, M. Karyana

{"title":"Validation of a Liquid Chromatography/Tandem Mass Spectrometry Assay for the Quantification of Plasma Dihydroartemisinin","authors":"Dona Arlinda, Intan Sari Oktoberia, M. Karyana","doi":"10.21705/mcbs.v5i3.194","DOIUrl":"https://doi.org/10.21705/mcbs.v5i3.194","url":null,"abstract":"Background: Insufficient plasma level of dihydroartemisinin (DHA) can select resistance and will further hinder malaria elimination program. We investigated clinical applicability of a validated liquid chromatography/tandem mass spectrometry (LC-MS/MS) assay to quantify plasma concentration of DHA in healthy subjects from a single oral administration of fixed dose combination of Dihydroartemisinin-Piperaquine.Materials and Methods: Micro-elution solid-phase extraction in a 96-well plate format was used to prepare the samples. DHA separation happened in Acquity UPLCTM BEH C18 column (50 × 2.1 mm, 1.7 µm). Mobile phase was a mixture of acetonitrile-ammonium acetate 10 mM pH 3.5 (50:50, v/v) at 0.3 mL/minute flow rate. Waters Acquity UPLC™ H-Class system coupled with triple quadruple mass spectrometry in positive electrospray ionization mode was used for detection. The internal standard was a stable isotope labelled DHA.Results: Calibration curve was linear with a correlation coefficient >0.995 over a concentration range of 1–1,000 ng/mL. Bias and variation for accuracy and precision were in the range of 15% (20% at the lower limit of quantification). Using 5 µL sample, lower limit of quantification was 1 ng. Matrix effect was less than 15%. The method was successfully applied to investigate the pharmacokinetics of DHA from five healthy subjects, although carry over and the role of anticoagulants were not tested.Conclusion: The LC-MS/MS assay for the quantification of plasma DHA was validated for selectivity, linearity, lower limit of quantitation, accuracy, precision, matrix effect and stability. Although clinical applicability was demonstrated, this method was to be improved to address the not-tested validation parameters.Keywords: dihydroartemisinin, liquid chromatography/tandem mass spectrometry assay (LC-MS/MS), malaria, Indonesia ","PeriodicalId":53387,"journal":{"name":"MCBS Molecular and Cellular Biomedical Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80992805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Escherichia coli and Klebsiella pneumonia as the Most Common Bacteria Causing Catheter Associated Urinary Tract Infection 大肠杆菌和肺炎克雷伯氏菌是引起导尿管相关性尿路感染的最常见细菌

MCBS Molecular and Cellular Biomedical Sciences Pub Date : 2021-11-01 DOI: 10.21705/mcbs.v5i3.218

Dwiyanti Puspitasari, Edward Adijaya Rusli, D. Husada, Leny Kartina

{"title":"Escherichia coli and Klebsiella pneumonia as the Most Common Bacteria Causing Catheter Associated Urinary Tract Infection","authors":"Dwiyanti Puspitasari, Edward Adijaya Rusli, D. Husada, Leny Kartina","doi":"10.21705/mcbs.v5i3.218","DOIUrl":"https://doi.org/10.21705/mcbs.v5i3.218","url":null,"abstract":"Background: Healthcare-Associated Infections (HAIs) are the result of a reaction between taint agents that infected the patient when the patient is hospitalized. A Study from The Center for Disease Control and Prevention shows that most HAIs in hospital are urinary tract infection, most of the infection incident in patient are caused by catheter. Catheter indwelling is notable in medical sphere. This study aimed to inquire case number of Catheter-Associated Urinary Tract Infection (CAUTI) in Dr. Soetomo General Hospital, the feature of CAUTI patients, the type of bacteria that cause CAUTI, and what is the relation among sex and bacteria colony.Materials and Methods: An analytic observational study with the population of pediatric hospitalized patients of Dr. Soetomo General Hospital was conducted in January-December 2017. Samples collected were positive urine culture from pediatric hospitalized patients. Information regarding the bacteria that cause CAUTI, gender, and length of catheter usage were collected.Results: There were total 140 samples of positive urine culture in pediatric patient, and 38.5% was diagnosed as CAUTI. Overall CAUTI was often found in male subjects (51.9 %), and similar with ≤1-year old patients which also often found in male subjects (60.8%). The highest length of catheter usage was 3-5 days (42.5%). All subjects had fever as a clinical sign and 83.3% had suprapubic pain. Escherichia coli and Klebsiella pneumoniae infections were highly discovered. There was an association between gender and urine culture colony count (p=0.02).Conclusion: CAUTI is commonnly found in Dr. Soetomo General Hospital, and two bacteria that cause the most infection were E. coli and K. pneumoniae.Keywords: catheter, urinary tract infection, healthcare associated infection","PeriodicalId":53387,"journal":{"name":"MCBS Molecular and Cellular Biomedical Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80707889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

The Stem Infusate and Ethanol Extract of Physalis angulata Inhibitory Activities against a-Glucosidase and Xanthine Oxidase 角Physalis茎浸膏和乙醇提取物对a-葡萄糖苷酶和黄嘌呤氧化酶的抑制活性

MCBS Molecular and Cellular Biomedical Sciences Pub Date : 2021-11-01 DOI: 10.21705/mcbs.v5i3.211

Anthony Tjajaindra, A. K. Sari, A. Simamora, K. Timotius

{"title":"The Stem Infusate and Ethanol Extract of Physalis angulata Inhibitory Activities against a-Glucosidase and Xanthine Oxidase","authors":"Anthony Tjajaindra, A. K. Sari, A. Simamora, K. Timotius","doi":"10.21705/mcbs.v5i3.211","DOIUrl":"https://doi.org/10.21705/mcbs.v5i3.211","url":null,"abstract":"Background: Infusate of the whole plant of Physalis angulata is used traditionally for the remedy of various diseases including diabetes and gout. This study focused on the stem of P. angulata. The objectives of this study were to investigate the potential of the stem infusate (INPA) and ethanol extract (EEPA) of P. angulata as inhibitors of α-glucosidase and xanthine oxidase.Materials and Methods: INPA and EEPA were determined for their α-glucosidase and xanthine oxidase inhibition activities in vitro, whereas antioxidant activity was determined by 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) assay. Reference inhibitors were used for comparison. The total phenolic compounds were also estimated.Results: EEPA had more concentrated phenolic than INPA which were 7.96 and 0.08 mgGAE/g dried biomass, respectively. INPA and EEPA inhibited α-glucosidase considerably, with IC50 of 149.11 and 409.86 µg/mL, respectively (acarbose was 130.66 µg/mL). However, they inhibited xanthine oxidase weakly, with IC50 of 0.546 and 2.643 mg/mL, respectively, compared with allopurinol 0.005 mg/mL. EEPA scavenged DPPH radicals very weakly (16.04 mg/mL) compared to BHT (0.021 mg/mL), whereas no activity was observed for INPA.Conclusion: The stem infusate and ethanol extract of P. angulata are able to inhibit the activity of α-glucosidase, thus can be further explored for sources of bioactive compounds with α-glucosidase inhibition activity.Keywords: α-glucosidase, infusate, ethanol extract, Physalis angulata, stem, xanthine oxidase","PeriodicalId":53387,"journal":{"name":"MCBS Molecular and Cellular Biomedical Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83269609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2