yfthhy nwyn dr `lwm zysty最新文献_第5页

Inhibition of esophageal cancer cell line (KYSE-30) proliferation using secondary metabolites of Ephedra endophyte bacteria 麻黄内生细菌次级代谢产物对食管癌细胞株KYSE-30增殖的抑制作用

yfthhy nwyn dr `lwm zysty Pub Date : 2021-07-01 DOI: 10.52547/nbr.8.2.95

M. Ghiasvand, A. Makhdoumi, Maryam Moghaddam Matin, J. Vaezi

{"title":"Inhibition of esophageal cancer cell line (KYSE-30) proliferation using secondary metabolites of Ephedra endophyte bacteria","authors":"M. Ghiasvand, A. Makhdoumi, Maryam Moghaddam Matin, J. Vaezi","doi":"10.52547/nbr.8.2.95","DOIUrl":"https://doi.org/10.52547/nbr.8.2.95","url":null,"abstract":"Regarding the rising rate of esophageal cancer in some parts of Asia, including northern and northeastern regions of Iran, the identification of new anti-cancer compounds is essential to combat the disease. The relation between some plants metabolites and these endophytic microorganisms are well characterized. In the current study, the potentials of Ephedra endophyte bacteria for the inhibition of esophageal cancer cell line (KYSE-30) proliferation were investigated. A total of 54 endophyte bacteria (out of 70) were obtained from the sterilized surfaces of two medicinal plants, i.e., Ephedra intermedia and Ephedra foliata. Bacterial strains were then cultured in Tryptic soy broth (TSB) medium and, after 72 h incubation, the produced secondary metabolites were extracted by chloroform. Anticancer effects of secondary metabolites from theses bacteria on esophageal cancer cell line KYSE-30 were evaluated after 24, 48 and 72 h by MTT method. MTT assay results showed that only strain A1 had a cytotoxic effect on KYSE-30 cells. The IC50 amounts of this strain against KYSE-30 cell lines were equaled (μg/ml) to 346.4, 192.8 and 121.3 after 24, 48, and 72 hours, respectively. The molecular identification of strain A1 revealed that Microbacterium maritypicum (99.8% Received 09.07.2020/ Revised 31.08.2020/ Accepted 19.11.2020/ Published 01.07.2021 :تفايرد ١٩ / ٤ ٠ / ٩ ١٣٩ / :حلاصا ٠ ١ / ٦ ٠ / ٩ ١٣٩ / پ :شريذ ٢٩ / ٨ ٠ / ٩ ١٣٩ / :راشتنا ١٠ / ٤ ٠ / ٤٠٠ ١ [ D O I: 1 0. 52 54 7/ nb r. 8. 2. 95 ] [ D O R : 2 0. 10 01 .1 .2 42 36 33 0. 14 00 .8 .2 .1 .2 ] [ D ow nl oa de d fr om n br .k hu .a c. ir o n 20 21 -1 124 ]","PeriodicalId":52900,"journal":{"name":"yfthhy nwyn dr `lwm zysty","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48013090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The floristic study of Golestankooh area in Isfahan Province, Iran 伊朗伊斯法罕省Golestankooh地区的区系研究

yfthhy nwyn dr `lwm zysty Pub Date : 2021-06-01 DOI: 10.52547/nbr.8.1.68

Azadeh Akhavan Roofigar, A. Bagheri

引用次数: 0

Molecular phylogeny and taxonomy of species of Cousinia section Stenocephalae (Asteraceae) in NE of Iran 伊朗东北部菊科（菊科）窄头虫科（Cousinia section Stenocephalae）物种的分子系统发育与分类

yfthhy nwyn dr `lwm zysty Pub Date : 2021-06-01 DOI: 10.52547/nbr.8.1.56

Simin Kalouti, S. Kazempour-Osaloo, F. Attar, A. Susanna, N. Garcia‐Jacas

引用次数: 0

The effect of exosomes derived from human ovarian epithelial cancer cells on the secretion of AMH and Inhibin in granulosa cells 人卵巢上皮癌症细胞外泌体对颗粒细胞分泌AMH和Inhibin的影响

yfthhy nwyn dr `lwm zysty Pub Date : 2021-06-01 DOI: 10.52547/nbr.8.1.31

N. Nikdel, J. Baharara, S. Zakerbostanabad, M. Tehranipour

{"title":"The effect of exosomes derived from human ovarian epithelial cancer cells on the secretion of AMH and Inhibin in granulosa cells","authors":"N. Nikdel, J. Baharara, S. Zakerbostanabad, M. Tehranipour","doi":"10.52547/nbr.8.1.31","DOIUrl":"https://doi.org/10.52547/nbr.8.1.31","url":null,"abstract":". Exosomes are secreted by different types of cells and known as biological packages. Exosomes have significant role in intercellular communications and involved in the development and progression of various diseases such as cancer. Inhibin B and anti-mullerian hormone (AMH) are markers of granulosa cell tumors (GCT) and due to the role of exosomes in the progression of cancer, in this experimental study, the effect of exosomes derived from human ovarian cancer cells on the secretion of Inhibin B and antimullerian hormone (AMH) by granulosa cells was investigated. First, A2780 human ovarian cancer cells were cultured, then the supernatant was collected to extract the exosomes by ultracentrifugation and subsequently, the extracted exosomes were checked out using dynamic light scattering (DLS) and Scanning electron microscopy (SEM). In addition, granulosa cells were isolated and cultured from the ovaries immature female Balb / C mice and treated with 25 μg/ml of exosomes derived from the ovarian cancer cell-line. Inhibin B and AMH hormones levels were then measured. The results showed the significant (P<0/05) increase of the level of inhibin B and AMH hormones in the treated cells in comparison with the control group. According to the results, exosomes increased the secretion of Inhibin B and AMH hormones and seems to be effective in the proliferation of granulosa cell tumors.","PeriodicalId":52900,"journal":{"name":"yfthhy nwyn dr `lwm zysty","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43930474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sexual dimorphism in digit length ratios of the second to fourth digits (2D:4D) in Blanford's fringe-toed lizard Acanthodactylus blanfordi Boulenger, 1918 (Sauria: Lacertidae) in the south of Iran 伊朗南部布兰福德缘趾蜥蜴布兰福德棘趾蜥第二趾与第四趾趾趾长度比的两性二态性(2D:4D)

yfthhy nwyn dr `lwm zysty Pub Date : 2021-06-01 DOI: 10.52547/nbr.8.1.46

Nastaran Heidari

{"title":"Sexual dimorphism in digit length ratios of the second to fourth digits (2D:4D) in Blanford's fringe-toed lizard Acanthodactylus blanfordi Boulenger, 1918 (Sauria: Lacertidae) in the south of Iran","authors":"Nastaran Heidari","doi":"10.52547/nbr.8.1.46","DOIUrl":"https://doi.org/10.52547/nbr.8.1.46","url":null,"abstract":"Finger length ratios are organized during embryonic development of fingers as they exposed to sex steroid hormones, and may show varying degrees of sexual dimorphism between males and females in different animal groups. Among all the finger length ratios calculated in a sample, the ratio between the second to fourth fingers (2D: 4D) is the most important one. In this study, the 2D:4D ratios in both sides of the body (right and left) in all limbs were investigated to determine if sexual dimorphism is present in the 2D: 4D ratios in 44 specimens studied (20 males and 24 females) of Acanthodactylus blanfordi. Other morphological traits of the two sexes were also examined (23 metric and meristic traits), as a result, sexual dimorphism was observed in five metric and meristic morphological traits. In terms of the ratio of the size of the fingers and toes, sexual dimorphism in the length of the fingers was observed only in 2D: 4D on the right side of the body in forelimbs and hindlimbs. The value of this trait was higher in males than females and this difference was statistically significant P ≤0.05).","PeriodicalId":52900,"journal":{"name":"yfthhy nwyn dr `lwm zysty","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42599528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prevalence and intensity of coccidian blood parasite infection in three species of lizards from Markazi Province, Iran 伊朗马尔卡齐省三种蜥蜴球虫血寄生虫感染的流行率和强度

yfthhy nwyn dr `lwm zysty Pub Date : 2021-06-01 DOI: 10.52547/nbr.8.1.39

Hossein Javanbakht, Reyhaneh Hajian

{"title":"Prevalence and intensity of coccidian blood parasite infection in three species of lizards from Markazi Province, Iran","authors":"Hossein Javanbakht, Reyhaneh Hajian","doi":"10.52547/nbr.8.1.39","DOIUrl":"https://doi.org/10.52547/nbr.8.1.39","url":null,"abstract":". Coccidian (Apicomplexa: Coccidia) parasites have been reported in almost all groups of vertebrates. These parasites may cause serious ecological and pathological effects to their hosts. Reptiles were found the be the host of the parasites of the genera Schellackia and Lankesterella, as these parasites undergo their entire life cycle in the reptilian body, with an intermediate stage of dormancy in the tissues of a hematophagus transmitter (generally a mite or a mosquito), until the next reptilian host swallows the infected insect. However, very little is known about the prevalence and intensity of these parasites in most of their distribution area, which is the focus of the current study. To do so, the prevalence and intensity of these blood parasites in three species of lizards, including Ophisops elegans , Trapelus lessonae and Eremias monticola from Markazi Province of Iran were investigated. Samples of blood were collected from the caudal vein of 51 lizards of the aforementioned species by insulin syringes, then the blood smears were dried, fixed with methanol and stained with Giemsa solution. Prepared blood samples were then examined by means of light microscopy, using a 100× magnification objective lens. 41 percent of the 51 examined samples were found to be infected by haemococcidians blood parasites from the family Lankesterellidae. The mean intensity of parasites in the three species were between 0.03-0.05%. The investigation revealed a relatively high percentage of prevalence and intensity of blood parasites in lizards of central part of Iran. The results showed the presence of a complete set of various vectors for the spread of haemoparasites in the area. Therefore, future researches are strongly recommended to utilize genetic markers in order to obtain a better identification of the parasites.","PeriodicalId":52900,"journal":{"name":"yfthhy nwyn dr `lwm zysty","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42956690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of antioxidant and anticancer effects of Lavandula angustifolia and Melissa officinalis on HeLa, OVCAR-3 and MCF-7 cancer cell lines 狭叶薰衣草和厚朴对HeLa、OVCAR-3和MCF-7癌症细胞系抗氧化和抗癌作用的评价

yfthhy nwyn dr `lwm zysty Pub Date : 2021-06-01 DOI: 10.52547/nbr.8.1.20

P. Hanachi, Hojat Sadeghi Ali Abadi, Nasim Ghorbani, Roshanak Zarringhalami, K. Kiarostami

{"title":"Evaluation of antioxidant and anticancer effects of Lavandula angustifolia and Melissa officinalis on HeLa, OVCAR-3 and MCF-7 cancer cell lines","authors":"P. Hanachi, Hojat Sadeghi Ali Abadi, Nasim Ghorbani, Roshanak Zarringhalami, K. Kiarostami","doi":"10.52547/nbr.8.1.20","DOIUrl":"https://doi.org/10.52547/nbr.8.1.20","url":null,"abstract":"From ancient times, plants have been regarded as therapeutic agents, in addition to their usage as food. Plants are rich sources of antioxidant and phenolic compounds. Lavandula angustifolia and Melissa officinalis are medicinal herbs rich in antioxidant compounds. The aim of this study was to compare the antioxidant and anticancer properties of Lavandula angustifolia and Melissa officinalis extracts using aqueous, ethanol and methanol solvents, to select the best extraction methods and solvents and to evaluate the cytotoxic effect of the extracts on HeLa, OVCAR-3 and MCF-7 cancer cell lines. Methanol, aqueous and ethanol extracts were obtained from the dried leaves of the plants and the antioxidant activities of each extract were Received 27.02.2020/ Revised 21.05.2020/ Accepted 07.06.2020/ Published 10.06.2021 :تفايرد ٠٨ / ١٢ / ١٣٩٨ :حلاصا / ٠١ / ٠٣ / ١٣٩٩ :شريذپ / ١٨ / ٠٣ / ١٣٩٩ :راشتنا / ٢٠ / ٠٣ / ١٤٠٠ [ D O I: 1 0. 52 54 7/ nb r. 8. 1. 20 ] [ D O R : 2 0. 10 01 .1 .2 42 36 33 0. 14 00 .8 .1 .1 0. 9 ] [ D ow nl oa de d fr om n br .k hu .a c. ir o n 20 21 -1 124 ]","PeriodicalId":52900,"journal":{"name":"yfthhy nwyn dr `lwm zysty","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43118295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

The effect of a VEGFB antagonist peptide on the expression level of miR-210 in a mouse model of breast cancer VEGFB拮抗肽对癌症小鼠模型中miR-210表达水平的影响

yfthhy nwyn dr `lwm zysty Pub Date : 2021-06-01 DOI: 10.52547/nbr.8.1.13

Fatemeh Kaboudan, S. Talesh Sasani, S. M. Asghari

{"title":"The effect of a VEGFB antagonist peptide on the expression level of miR-210 in a mouse model of breast cancer","authors":"Fatemeh Kaboudan, S. Talesh Sasani, S. M. Asghari","doi":"10.52547/nbr.8.1.13","DOIUrl":"https://doi.org/10.52547/nbr.8.1.13","url":null,"abstract":". Breast cancer is the fourth common cancer worldwide and occurs when breast cells begin to uncontrolled division and tumor formation. Angiogenesis is one of the essential factors in cell growth and maintenance of homeostasis in the natural and pathological conditions, while VEGFs are the most critical factors in angiogenesis. MiR-210 plays an important role in the angiogenesis via association with VEGF. Here, the miR-210 expression changes in response to a VEGFB antagonist peptide, called VEGB1, was studied in female BALB/c mice bearing 4T1 cell line induced breast tumor. The treated group received 1mg.kg -1 and 10mg.kg -1 of the peptide and the control group received PBS intraperitoneally during two weeks. Both of the animal groups underwent a resection of breast tissue 14 days after treatment and miR-210 expression level was investigated. Statistical analysis by On-way ANOVA showed that the expression level of miR-210 gene had significant differences among the groups treated with various doses of VEGB1. Also, the gene expression was significantly different between peptide-treated groups and control samples ( p <0.05). MiR-210 expression level had 42% reduction in mice treated with 1mg.kg -1 of VEGB1, while 90% was seen in mice treated with 10mg.kg -1 of VEGB1 showing the inhibitory function of VEGB1 antagonist peptide at different doses.","PeriodicalId":52900,"journal":{"name":"yfthhy nwyn dr `lwm zysty","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46097271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A new insight to the phytochemical and morphological diversity in commercial pomegranate (Punica granatum) cultivars 石榴（Punica granatum）商品品种植物化学和形态多样性的新认识

yfthhy nwyn dr `lwm zysty Pub Date : 2021-02-01 DOI: 10.52547/nbr.7.4.442

Shiva Shahsavari, Z. Noormohammadi, M. Sheidai, F. Farahani, Mohammad Reza Vazifeshenas

{"title":"A new insight to the phytochemical and morphological diversity in commercial pomegranate (Punica granatum) cultivars","authors":"Shiva Shahsavari, Z. Noormohammadi, M. Sheidai, F. Farahani, Mohammad Reza Vazifeshenas","doi":"10.52547/nbr.7.4.442","DOIUrl":"https://doi.org/10.52547/nbr.7.4.442","url":null,"abstract":". The pomegranate fruit is one of the most important horticultural products due to the presence of polyphenolic, antioxidant and anti-fungal compounds. In the present study, morphological and phytochemical compounds diversity was investigated in eight Iranian commercial pomegranate cultivars. Fourteen morphological characters and 10 chemical compounds were measured. Based on PCA analysis the most variable morphological characteristics among Punica cultivars studied were the wood surface, the leaf tip, the leaf incision, growth power and rained condition. High-performance liquid chromatograms of chlorgenic acid, caffeic acid, Para Coumaric acid as well as titrable acidity, total soluble solid, total phenolic contents, antioxidant activity in juice and peel of cultivars differentiated the cultivars studied. Comparing the two UPGMA dendrograms of Punica cultivars based on morphological data and chemical contents indicated certain disagreement between them. The correlation between morphological and chemical compounds and geographical distances of Punica cultivars was not statistically significant by the Mantel test. These findings indicate that Punica cultivars studied differ in their genetic content, however, this genetic difference is not correlated with their geographical distance.","PeriodicalId":52900,"journal":{"name":"yfthhy nwyn dr `lwm zysty","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48639779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The enhancing effect of electromagnetic field on the expression of Oligodendrocyte transcription factor 1 and 2 (Olig1/2) in the mice cerebral cortex 电磁场对小鼠大脑皮层少突胶质细胞转录因子1和2 (Olig1/2)表达的增强作用

yfthhy nwyn dr `lwm zysty Pub Date : 2021-02-01 DOI: 10.52547/nbr.7.4.374

F. Mashayekhi, S. Shabani, S. Talesh Sasani, Z. Salehi

{"title":"The enhancing effect of electromagnetic field on the expression of Oligodendrocyte transcription factor 1 and 2 (Olig1/2) in the mice cerebral cortex","authors":"F. Mashayekhi, S. Shabani, S. Talesh Sasani, Z. Salehi","doi":"10.52547/nbr.7.4.374","DOIUrl":"https://doi.org/10.52547/nbr.7.4.374","url":null,"abstract":". Olig1 and Olig2, two transcription factors, play regulatory function in the differentiation and specification of oligodendrocyte progenitor cells (OPCs). In this study the effects of electromagnetic fields (EMF) on total protein concentration (TPC) and Olig1 and Olig2 expression in the cerebral cortex of mouse was examined. Twenty-one Balb/c mice were separated into three groups: control, EMF and Sham groups (n=7 for each group). The mice were placed inside the solenoid for a daily EMF exposure of 50 Hz, 1 mT for 6 h/day, 7 days/week for 10 days. The Sham group was also located in the same coil with no exposure. Mice were anesthetized after the final exposure session and their cerebral cortex were collected. TPC and the expression of Olig 1 and Olig2 were studied by Bio-Rad protein assay and western blot, respectively. The cerebral cortex samples were removed for further analysis. There was no significant difference in TPC in the EMF treated cortical samples as compared with those from the SHAM and control groups. It was also shown that the expression of Olig1 and Olig2 was increased in the EMF treated cortical extracts as compared with those in controls and SHAM groups. Therefore, it could be concluded that EMF enhances Olig1 and Olig2 expression in the mice cerebral cortex. Moreover, as Olig1 and Olig2 plays important role in the development of oligodendrocyte progenitor cells, it can be deduced that EMF may affect OPC differentiation by increasing the expression of Olig1 and Olig2. Further studies are needed to clarify the extent of EMF impact on oligodendrocyte differentiation.","PeriodicalId":52900,"journal":{"name":"yfthhy nwyn dr `lwm zysty","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44360094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0