Problemy Osobo Opasnykh Infektsii最新文献

{"title":"Comparative Analysis of Expression of the Main Virulence Genes in Various Vibrio cholerae О1 Strains","authors":"N. B. Cheldyshova, Z. Devdariani","doi":"10.21055/0370-1069-2022-3-151-157","DOIUrl":"https://doi.org/10.21055/0370-1069-2022-3-151-157","url":null,"abstract":"The aim of the work was a comparative study of the expression of the main virulence genes in Vibrio cholerae strains of the classical biovar, typical and genetically modified strains of V. cholerae, El Tor biovar.Materials and methods. Natural toxigenic strains of V. cholerae O1, classical biovar (J89, Pakistan, 1969), typical (M-887, Astrakhan, 1970) and genetically modified (301, Taganrog, 2011) strains of the El Tor biovar were used as model ones. The strains were grown under optimum conditions for the production of cholera toxin and toxin-coregulated pili. The assessment of strain growth was carried out in LB broth at room temperature with determination of the cells number on a Biowave DNA spectrophotometer (Biochrome Ltd., UK). Determination of gene expression was performed using real-time PCR with reverse transcription.Results and discussion. The expression of structural (ctxA, tcpA) and regulatory (toxR, toxT, tcpP, tcpH) virulence genes has been investigated in V. cholerae strains of the classical biovar, typical and genetically modified strains of the El Tor biovar. Significant differences have been revealed in terms of time and level of maximum expression of these genes in strains of classical and El Tor biovars. It was found that ctxA and toxR genes expression in the genovariant strain reached its maximum 1–3 h earlier than in the other strains. At the same time, the level of ctxA gene expression corresponded to the level of the classical strain. The maximum expression of the toxR gene in the genovariant strain was higher than in typical El Tor and classical strains, and also had a clear inverse correlation with ctxA gene expression. Expression of the tcpA, toxT, and tcpH genes in the classical biovar strain reached its maximum 1–2 h earlier than in the El Tor biovar strains. These differences should be taken into account when conducting research work related to the study of the expression of the main virulence genes.","PeriodicalId":52264,"journal":{"name":"Problemy Osobo Opasnykh Infektsii","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47395303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Improvement of Approaches to the Verification of the Vaccine Strain Francisella tularensis 15 NIIEG during Long-Term Storage","authors":"L. V. Sayapina, N. A. Osina, E. A. Naryshkina, A. Fedorov, Ya. M. Krasnov, D. S. Davydov, V. Bondarev","doi":"10.21055/0370-1069-2022-3-137-144","DOIUrl":"https://doi.org/10.21055/0370-1069-2022-3-137-144","url":null,"abstract":"The aim of the study was to improve the methods for verifying the vaccine strain Francisella tularensis 15 NIIEG during long-term storage under current conditions.Materials and methods. The paper summarizes the results of studying the phenotypic and genetic properties of lyophilized cultures of the vaccine strain F. tularensis 15 NIIEG (1953, 1966, 1969, 1987, 1990, 2003, 2012 and 2013) stored at SCEMAP for a period of one to 60 years.Results and discussion. Previous studies have revealed that freeze-dried cultures of F. tularensis 15 NIIEG generally had the characteristics of the vaccine strain, with the exception of deviations from the regulatory requirements for residual virulence and specific safety. The stability of preservation of deletions in the pilA and pilE genes (the region of differentiation RD19) and the genes encoding lpp lipoprotein (RD18) in the vaccine strain, which was stored for various periods of time in a lyophilized state, has been confirmed. The vaccine-strain-specific mutation C178404T (by the genome of F. tularensis LVS strain, GenBank NCBI no. CP009694) has been identified, and an approach to determine it has been developed. The data obtained are promising as regards using the above deletions in the RD18/RD19 regions in combination with the C178404T mutation to assess the authenticity of the vaccine strain using molecular genetic methods. Thus, the conducted retrospective analysis of the data on the cultures of tularemia microbe vaccine strain from the 1940s to 2013 and the gathered experimental data, made it possible to supplement the uniform requirements for the manufacture, study, maintenance, storage and movement of F. tularensis 15 NIIEG vaccine strain with new evidence. Based on the results obtained, the authors have drawn a draft methodological recommendations of the federal level “Vaccinal strain Francisella tularensis 15 NIIEG: order of handling”.","PeriodicalId":52264,"journal":{"name":"Problemy Osobo Opasnykh Infektsii","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45521295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pathogenicity of the SARS-CoV-2 Virus Variants of Concern for the Syrian Golden Hamster","authors":"A. Shipovalov, G. Kudrov, A. Tomilov, S. Bodnev, N. D. Boldyrev, A. S. Ovchinnikova, A. V. Zaikovskaya, O. Taranov, E. Ivleva, O. P’yankov, R. Maksyutov","doi":"10.21055/0370-1069-2022-3-164-169","DOIUrl":"https://doi.org/10.21055/0370-1069-2022-3-164-169","url":null,"abstract":"The aim of the work was to study the pathogenicity of newly emerging variants of SARS-CoV-2 on the model of the Syrian golden hamster.Materials and methods. We used the strains of SARS-CoV-2 virus related to the VOC circulating in the territory of the Russian Federation. The experiments were carried out on outbreed Syrian hamsters obtained from the nursery of the SSC VB “Vector”. The infectious titer of coronavirus in tissue samples collected from infected laboratory animals was determined on a Vero E6 cell culture. The Ct in RT-PCR was considered an additional parameter for monitoring the viral load in the samples. The severity of lung tissue damage in Syrian hamsters with COVID-19 was assessed by histological preparations.Results and discussion. 50 % infecting doses in case of the intranasal infection have been determined, histological analysis of lung tissues performed. The pathogenicity of various variants of the SARS-CoV-2 virus for the Syrian hamster has been evaluated, differences in infecting doses and pathological changes in the lungs have been revealed. SARS-CoV-2 viruses belonging to Beta genetic variant have the highest virulence, while Alpha variant has the lowest one when comparing the studied strains by the ID50 value. The Delta and Omicron variants have a matched ability to cause specific damage to the tissues of the respiratory tract, while being inferior only to the Beta variant. It has been demonstrated that Syrian hamsters are an adequate model for assessing the pathogenicity of the SARS-CoV-2 virus variants of concern. Variants of SARS-CoV-2 virus during intranasal infection has shown different degree of pathogenicity in the Syrian hamster model.","PeriodicalId":52264,"journal":{"name":"Problemy Osobo Opasnykh Infektsii","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46492547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anthrax in the Republic of Tatarstan (1920–2020)","authors":"T. Savitskaya, V. Trifonov, I. V. Milova, G. Isaeva, I. Reshetnikova, I. V. Serova, D. V. Lopushov, V. B. Ziatdinov","doi":"10.21055/0370-1069-2022-3-129-136","DOIUrl":"https://doi.org/10.21055/0370-1069-2022-3-129-136","url":null,"abstract":"The aim of the work was to characterize the epidemiological and epizootic situation on anthrax among population and animals in the Republic of Tatarstan over a period of 1920–2020.Materials and methods. The analysis of the epidemiological and epizootic situation is based on the archival data, epidemiological maps of anthrax patients, results of epizootiological-epidemiological survey of anthrax foci conducted by the Rospotrebnadzor Administration in the Republic of Tatarstan and Center of Hygiene and Epidemiology in the Republic of Tatarstan, materials of the Main Directorate of Veterinary Medicine of the Republic of Tatarstan. Microbiological studies of samples from patients and environmental objects were performed in accordance with the requirements of MR 4.2.2413-08 “Laboratory diagnostics and detection of anthrax pathogen”, real-time PCR was set using the AmpliSense Bacillus anthracis-FRT test-system (Central Research Institute of Epidemiology, Moscow). Statistical data processing was carried out using the quantile ranking method.Results and discussion. There are more than 1000 anthrax soil foci in the Republic of Tatarstan, which territorially belongs to the Volga Federal District. Analysis of the epizootic and epidemiological situation in the Republic of Tatarstan over the period of 1920–2020 has revealed that it has undergone significant changes, from mass diseases in animals and humans in early 20th century to sporadic cases of infection among population and animals at the beginning of the 21st century, primarily due to preventive veterinarysanitary measures, including veterinary and sanitary examination of animal products, mass specific immunization of animals against anthrax, arrangement of anthrax cattle burial grounds. In view of the improvement of epizootiological situation and implementation of preventive measures, there was a decrease in the incidence of anthrax among the population. The regions of the Republic have been ranked by the number of animal anthrax cases.","PeriodicalId":52264,"journal":{"name":"Problemy Osobo Opasnykh Infektsii","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48779950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Real-Time PCR Detection of Bacillus anthracis by Lambda_Ba03 Prophage Genes","authors":"A. Nizkorodova, E. R. Mal’tseva, Z. Berdygulova, D. A. Naizabaeva, S. Kuatbekova, A. Zhigailov, N. Abdolla, A. Mashzhan, I. A. Akhmetollaev, Y. Skiba, S. Mamadaliev","doi":"10.21055/0370-1069-2022-3-170-172","DOIUrl":"https://doi.org/10.21055/0370-1069-2022-3-170-172","url":null,"abstract":"The aim of the study was to develop a set of primers and fluorescent probes for the detection of two chromosomal targets of Bacillus anthracis using real-time PCR based on the lambda_Ba03 prophage genes.Materials and methods. BLAST analysis of B. anthracis chromosomal DNA identified two target genes in the region of lambdaBa03 prophage, BA_5358 (AE016879.1: 4852332..4853642) and BA_5361 (AE016879.1: 4855298..4856278). The designed primers and fluorescent hydrolysable TaqMan probes for simultaneous detection of B. anthracis chromosomal DNA by two stated genes were tested in qPCR for sensitivity and specificity.Results and discussion. Studies performed on chromosomal DNA samples of closely related bacteria (B. cereus, B. thuringiensis, B. subtilis, B. clausii) have shown 100 % specificity of the developed sets of primers/probes. The sensitivity of the devised multiplex kit, tested on DNA samples of the m55-VNIIVViM vaccine strain and archival DNA samples of B. anthracis, reached 100 fg of bacterial DNA, which sets the limit of sensitivity at 17 genomes per reaction. The developed multiplex kit can be used as a separate tool for research laboratories studying anthrax.","PeriodicalId":52264,"journal":{"name":"Problemy Osobo Opasnykh Infektsii","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48015037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effectiveness of MALDI ToF Mass Spectrometry in Identification of Francisella tularensis Strains","authors":"A. K. Syngeeva, A. Ostyak, E. S. Kulikalova, A. Mazepa, K. Naumova, S. Balakhonov","doi":"10.21055/0370-1069-2022-3-145-150","DOIUrl":"https://doi.org/10.21055/0370-1069-2022-3-145-150","url":null,"abstract":"The aim of the study was to evaluate the effectiveness of MALDI‑ToF mass spectrometry in the identification of collection and newly isolated strains of tularemia pathogen using the database “Protein profiles of mass spectra of microorganisms belonging to I–II pathogenicity groups for the MALDI Biotyper software”.Materials and methods. We investigated 142 strains of Francisella tularensis, including 59 collection strains and 83 newly isolated ones. Bacteriological, molecular-genetic and proteomic research methods were used to identify them. The acquisition of mass spectra, analysis, generation and expansion of reference libraries were performed on a mass analyzer “Microflex LT” using FlexControl v. 3.3, FlexAnalysis v. 3.3, and MALDI Biotyper 3.0 software packages. The cluster analysis was performed using the BioNumerics 7.6 software.Results and discussion. The possibility of identifying tularemia pathogen has been assessed using the extended database for MALDI Biotyper 3.0 “Protein profiles of mass spectra of microorganisms belonging to I–II pathogenicity groups for the MALDI Biotyper software”. During identification to the species level, the significance of mass spectrometry results for collection strains and newly isolated ones was 91.5 % and 97.6 %, respectively. In determining the genus appurtenance, the reliability of identification was 100 %. Thus, the MALDI‑ToF mass spectrometry method allows for accurate species and genus identification of F. tularensis strains. Based on the cluster analysis of 66 F. tularensis strains in BioNumerics 7.6 software using «Pearson correlation» and the UPGMA algorithm, the possibility of subspecies differentiation has been evaluated. Due to the similarity of protein profiles of F. tularensis strains, a clear differentiation into subspecies could not be achieved. It is necessary to use other options for sample preparation, new generation devices with higher resolution, as well as apply additional approaches and analysis tools for successful subspecific differentiation.","PeriodicalId":52264,"journal":{"name":"Problemy Osobo Opasnykh Infektsii","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42102320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Experience in Using Mobile Laboratory for Monitoring and Diagnostics in the Socialist Republic of Vietnam","authors":"Zh. A. Kas’yan, L. T. Anh, I. Sharova, V. Cuong, E. G. Oglodin, T. Toan, S. N. Golubev, M. V. Proskuryakova, Bui Thi Lan Anh, Hoang Duc Hau, Dang Thi Viet Huong, Pham Thi Ha Giang, Duong Van Nghia, B. T. Nga, M. N. Lyapin, S. A. Shcherbakova","doi":"10.21055/0370-1069-2022-3-90-94","DOIUrl":"https://doi.org/10.21055/0370-1069-2022-3-90-94","url":null,"abstract":"The aim was to present the experience of using mobile laboratory for monitoring and diagnostics (MLMD) during the epizootiological monitoring of the northern provinces of Vietnam. MLMD was transferred by Federal Service for Surveillance in the Sphere of Consumers Rights Protection and Human Welfare to the Socialist Republic of Vietnam as part of implementation of cooperation programs on combating infectious diseases. The use of MLMD made it possible to obtain new information on the circulation of pathogens of natural-focal infectious diseases on the territory of Vietnam. It also provided the necessary conditions for conducting research using methods of express diagnostics, bacteriological analysis, performing a full cycle of work – from the receipt of samples to the disinfection and destruction of infected material in compliance with the requirements of biological safety in the field. The effectiveness of using mobile laboratories in response to the emergencies of sanitary and epidemiological nature, both to strengthen stationary laboratory bases and to organize diagnostic studies in remote regions, has been shown. The use of MLMD for the diagnosis of COVID‑19 has been an effective component of countering the new coronavirus infection in Vietnam and significantly increased the volume of testing in the country.","PeriodicalId":52264,"journal":{"name":"Problemy Osobo Opasnykh Infektsii","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42662875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Yersinia pestis Strains of the 1.ORI Line as Etiological Agent of the Plague Pandemic III","authors":"K. A. Nikiforov","doi":"10.21055/0370-1069-2022-3-23-37","DOIUrl":"https://doi.org/10.21055/0370-1069-2022-3-23-37","url":null,"abstract":"Yersinia pestis strains of the 1.ORI lineage originate from China as a result of evolution of the 1.ANT phylogenetic branch. Strains of the biovar orientalis are divided into three major lines of evolution: 1.ORI1, 1.ORI2, 1.ORI3. Lines 1.ORI1 and 1.ORI2 originated in China and then spread across the east and west coasts of India, respectively. Strains of the biovar orientalis have widely spread throughout the world, mainly as a result of introduction by sea. This way, the 1.ORI1 line was imported onto the territory of North America. 1.ORI2 line has spread to Southeast Asia, Africa, Europe, and South America. In addition, the strains of the biovar orientalis were brought to the territory of Australia, however, the formation of natural foci did not occur. The spread of strains to new territories during the third plague pandemic, as a rule, took place with the participation of one strain, which caused epizootics among synanthropic rodents. After that, outbreaks were recorded among the population of port cities, followed by drifting into the countryside and the formation of natural foci under suitable natural conditions. In the absence of such, the plague pathogen was eliminated from natural biotopes, and the formation of a natural focus did not occur. In recent decades, most cases of human plague in the world have been caused by strains of the biovar orientalis (1.ORI). However, the emergence and spread of the evolutionary line “1” is insufficiently studied. Currently, there is a lack of both historical data and strains that are ancestors of modern strains in many countries to clarify the details of the irradiation of strains of the biovar orientalis. As a result, the concepts of dissemination of many evolution branches of the strains, biovar orientalis are in the form of hypotheses to date. In this work, the collection and analysis of literature data on the history and epidemiology of plague over the third pandemic, a search for a connection between epidemic manifestations and the appurtenance of the strains that caused them to certain phylogenetic lineages was carried out.","PeriodicalId":52264,"journal":{"name":"Problemy Osobo Opasnykh Infektsii","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49193023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibiotic Resistance of Uropathogenic Escherichia coli Isolated from Patients with Urinary Tract Infections at the Urological Inpatient Facility of the Saratov Clinical Hospital","authors":"A. V. Kazantsev, M. V. Proskuryakova, E. S. Kazakova, N. A. Osina, I. Shvidenko, A. N. Mikerov","doi":"10.21055/0370-1069-2022-3-82-89","DOIUrl":"https://doi.org/10.21055/0370-1069-2022-3-82-89","url":null,"abstract":"The aim of the work was to study the profile of antibiotic resistance of uropathogenic Escherichia coli strains isolated from patients with urinary tract infections in the urological inpatient facility of the clinical hospital in the Saratov city, depending on appurtenance to phylogenetic groups and subgroups, as well as O-serogroups.Materials and methods. We assessed sensitivity/resistance to 25 different antibacterial drugs in 102 strains of uropathogenic E. coli. The studies were carried out using the disk diffusion method. The production of extended spectrum beta-lactamases was evaluated by the double disk method. Carbapenemase output was determined using the CIM test. The PCR method was applied to determine appurtenance to phylogenetic groups and subgroups, O-serogroups, as well as the frequency of occurrence of the mcr‑1, mcr‑2, mcr‑3, mcr‑4, mcr‑5 genes encoding the proteins that mediate the development of resistance to colistin.Results and discussion. It has been established that all strains of uropathogenic E. coli are more or less resistant to antibacterial drugs. All studied 102 strains showed resistance to 23 antibacterial drugs from 8 functional groups. The resistance of uropathogenic E. coli had certain differences depending on belonging to phylogenetic groups and subgroups, O-serogroups. Strains of uropathogenic E. coli with high resistance (up to 100 %) belonged to the B23 phylogenetic group, the main representatives of which are cultures of the most common O-25 serogroup. The production of extended-spectrum beta-lactamases has been phenotypically confirmed for 69 (67.6 %) strains. No carbapenemaseproducing cultures were found in the study. The mcr‑1 and mcr‑2 genes encoding resistance to colistin have been identified in 3 uropathogenic E. coli strains (2.9 %).","PeriodicalId":52264,"journal":{"name":"Problemy Osobo Opasnykh Infektsii","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44739778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exposure of Food Samples to Pulsed Microwave Radiation to Increase their Microbiological Safety and Shelf Life","authors":"Y. Gulyaev, V. P. Meshchanov, B. M. Kats, N. A. Koplevatsky, A. A. Lopatin, K. Sayapin, V. Elkin, V. Komarov, V. Bayburin, A. Rytik","doi":"10.21055/0370-1069-2022-3-70-74","DOIUrl":"https://doi.org/10.21055/0370-1069-2022-3-70-74","url":null,"abstract":"The aim of the study was to increase the efficiency of decontamination of biological material and media (by the example of food products) by pulsed (non-thermal) radio emission and asses the prospects of its application in medicine and biology.Materials and methods. To achieve the goal an experimental setup has been designed, manufactured and tested, which makes it possible to study the process of exposure of biological materials and media to pulsed (non-thermal) radio emission, in particular, by the example of food products. The basis of the method is optimum control of the electro-physical parameters of the irradiating radio signal, depending on the type of the irradiated object. We used pulsed magnetrons with operating frequency of (2.45±0.05) GHz, authorized for bio-medical research, generating pulsed radiation with an adjustable power within the range of 0.1...10 kW. The pulse repetition rate with a duty cycle of 500...10000 is 0.1...5 kHz. The setup has an operating chamber into which the test sample is placed, as well as additional elements of magnetron protection and measuring the parameters of the microwave power incident on biological object.Results and discussion. The setup has been successfully used to irradiate various food samples with pathogenic micro flora (Salmonella spp. etc.) with pulsed microwave radiation. In particular, as shown by the studies, the arithmetic mean number of pathogenic bacteria in the irradiated samples of minced meat decreased by 27.5 times after 28 days of storage as compared to the control group of non-irradiated samples. Preliminary conducted experiments in the field of investigating the effect of microwave radiation on the process of cell division and other aspects of electromagnetic field influence on pathological microorganisms confirm the prospects and the expediency of continuing the ongoing studies in medicine and biology. ","PeriodicalId":52264,"journal":{"name":"Problemy Osobo Opasnykh Infektsii","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46777604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}