NPJ science of plants最新文献

Streamlined molecular farming of plant virus therapeutics for space flight and other low-resource environments. 用于太空飞行和其他资源匮乏环境的植物病毒治疗的流线型分子养殖。

NPJ science of plants Pub Date : 2026-01-01 Epub Date: 2026-06-05 DOI: 10.1038/s44383-026-00030-y

Patrick Opdensteinen, Kyle Lewin, Anshal Jain, Andrew Copeland, Jonathan Copeland, Maziar Ghazinejad, Nicole F Steinmetz

{"title":"Streamlined molecular farming of plant virus therapeutics for space flight and other low-resource environments.","authors":"Patrick Opdensteinen, Kyle Lewin, Anshal Jain, Andrew Copeland, Jonathan Copeland, Maziar Ghazinejad, Nicole F Steinmetz","doi":"10.1038/s44383-026-00030-y","DOIUrl":"10.1038/s44383-026-00030-y","url":null,"abstract":"The resupply of pharmaceuticals during long-term space missions is challenging due to long travel distances and the reduced shelf-life of pharmaceuticals under extraterrestrial conditions. On-demand pharmaceutical production using plants could address this limitation. Plants are already cultivated in space, but the production of pharmaceuticals in plants is hampered by traditionally complex purification processes. Here, we describe a simplified production and purification strategy for cowpea mosaic virus (CPMV), a plant virus-based therapeutic candidate with strong immunomodulatory properties suitable for cancer therapy and vaccine development. By combining vacuum infiltration and centrifugation, intact CPMV particles were recovered from the apoplast without damaging the plant tissue, eliminating the need for tissue disruption. Impurities in apoplast eluates were efficiently removed by ultrafiltration/diafiltration, exploiting the size difference between CPMV and contaminants. The process was scalable when applied to more than 50 plants. We assessed the robustness of this production process under simulated space conditions, including microgravity, temperature shifts, and exposure to reactive oxygen species (ROS). Microgravity altered plant morphology, whereas temperature changes and ROS stress affected CPMV yields in a time-dependent manner. Beyond applications in space, these findings enable terrestrial strategies for plant molecular farming in low-resource environments.","PeriodicalId":520932,"journal":{"name":"NPJ science of plants","volume":"2 1","pages":"16"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13241316/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148202127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transforming viticulture through genomic innovation and integrated pest management for sustainable grape production. 通过基因创新和病虫害综合治理改造葡萄栽培，实现可持续葡萄生产。

NPJ science of plants Pub Date : 2026-01-01 Epub Date: 2026-08-04 DOI: 10.1038/s44383-026-00038-4

Giorgio Gambino, Lara Agnoli, Walter Chitarra, Marc Dressler, Andreia Figueiredo, Mario de la Fuente, Gabriele Di Gaspero, Ludger Hausmann, Christos Karatzas, Benoit Laurent, Pere Mestre, Irene Perrone, Komlan Avia

引用次数: 0

Hot and Hungry - High temperatures induce changes in leaf carbon dynamics and sugar isotope fingerprints. 高温诱导叶片碳动态和糖同位素指纹的变化。

NPJ science of plants Pub Date : 2025-01-01 Epub Date: 2025-12-01 DOI: 10.1038/s44383-025-00012-6

Philipp Schuler, Margaux Didion-Gency, Valentina Vitali, Matthias Saurer, Manuela Oettli, Haoyu Diao, Nina Buchmann, Arthur Gessler, Marco M Lehmann

{"title":"Hot and Hungry - High temperatures induce changes in leaf carbon dynamics and sugar isotope fingerprints.","authors":"Philipp Schuler, Margaux Didion-Gency, Valentina Vitali, Matthias Saurer, Manuela Oettli, Haoyu Diao, Nina Buchmann, Arthur Gessler, Marco M Lehmann","doi":"10.1038/s44383-025-00012-6","DOIUrl":"10.1038/s44383-025-00012-6","url":null,"abstract":"Predicting vegetation responses to global warming and reconstructing them from stable isotope records requires a clear understanding of how they respond to temperature. We investigated leaf carbon-dynamics (gas exchange, non-structural carbohydrate (NSC)) and the hydrogen (δ²H) and oxygen (δ¹⁸O) isotopic composition of leaf water and sugars in well-watered C₃ trees, forbs, grasses, and one C₄ grass across air temperatures from 10 °C to 40 °C at a low VPD. In C₃ species, temperatures ≥30 °C reduced Anet, increased Rdark, and shifted NSC composition from starch to sugars. Concurrently, apparent δ²H and δ¹⁸O fractionation between leaf water and sugars decreased. δ²H and δ¹⁸O in C₃ plants can be modeled from leaf water isotope composition when accounting for temperature-driven changes in gas exchange and NSC dynamics. These findings reveal heat-induced shifts in carbohydrate metabolism leave distinct isotope signatures, providing a mechanistic basis for improved isotope models to study current and past tree carbon dynamics.","PeriodicalId":520932,"journal":{"name":"NPJ science of plants","volume":"1 1","pages":"12"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12669037/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145673495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PDLP5, a plasmodesmata permeability regulator, can traffic between plant cells. PDLP5是一种间连丝通透性调节因子，可在植物细胞间进行交通。

NPJ science of plants Pub Date : 2025-01-01 Epub Date: 2025-07-01 DOI: 10.1038/s44383-025-00001-9

Yumin Kan, Vitaly Citovsky

{"title":"PDLP5, a plasmodesmata permeability regulator, can traffic between plant cells.","authors":"Yumin Kan, Vitaly Citovsky","doi":"10.1038/s44383-025-00001-9","DOIUrl":"10.1038/s44383-025-00001-9","url":null,"abstract":"Plasmodesmata (PD) are membranous nanopores that connect the cytoplasm of neighboring plant cells and enable the cell-to-cell trafficking of nutrients and macromolecules, as well as invading viruses. PD plays a critical role in regulating intercellular communication, contributing to plant development, environmental responses, and interactions with viral pathogens. The PD proteome includes two major types of functional proteins, PD callose binding proteins (PDCBs) and PD-located proteins (PDLPs), involved in manipulating the size of PD pores. Most studies of PDCBs and PDLPs focused on their effects on the PD transport of different cargo molecules; yet, whether these PD proteins themselves have the capacity for cell-to-cell movement remains largely obscure. Here, we addressed this question by demonstrating that PDLP5, but not PDLP1 and PDLP2, can move efficiently from cell to cell in Nicotiana benthamiana and that this movement involves the native transmembrane domain of PDLP5. These observations would be useful for a better understanding of the complexity of the PDLP5 protein function during the processes of PD transport.","PeriodicalId":520932,"journal":{"name":"NPJ science of plants","volume":"1 1","pages":"1"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12236089/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144593834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Historical and mechanistic perspective on ABP1-TMK1-mediated cell surface auxin signaling. abp1 - tmk1介导的细胞表面生长素信号传导的历史和机制研究。

NPJ science of plants Pub Date : 2025-01-01 Epub Date: 2025-07-01 DOI: 10.1038/s44383-025-00002-8

Aline Monzer, Jiří Friml

引用次数: 0

Rethinking the interpretation of spring phenological temperature sensitivity. 对春季物候温度敏感性解释的再思考。

NPJ science of plants Pub Date : 2025-01-01 Epub Date: 2025-08-04 DOI: 10.1038/s44383-025-00004-6

Manuel G Walde, Yann Vitasse, E M Wolkovich

引用次数: 0