Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie最新文献

{"title":"The Sting of Rejection: Deferring Blood Donors due to Low Hemoglobin Values Reduces Future Returns.","authors":"Adrian Bruhin,&nbsp;Lorenz Goette,&nbsp;Simon Haenni,&nbsp;Lingqing Jiang,&nbsp;Alexander Markovic,&nbsp;Adrian Roethlisberger,&nbsp;Regula Buchli,&nbsp;Beat M Frey","doi":"10.1159/000500679","DOIUrl":"https://doi.org/10.1159/000500679","url":null,"abstract":"<p><strong>Background: </strong>Roughly one quarter of short-term temporary deferrals (STTD) of blood donors are low-hemoglobin deferrals (LHD), i.e. STTD due to a hemoglobin (Hb) value falling below a cutoff of 125 g/L for female and 135 g/L for male donors. Since voluntarily donating blood is a prosocial activity, donors may perceive deferral as social exclusion, which can cause social pain, decrease self-esteem, and lead to antisocial behavior. However, little is known about the causal impacts of LHD on donor return.</p><p><strong>Study design and methods: </strong>We conducted a quasi-experiment with 80,060 donors invited to blood drives in the canton of Zurich, Switzerland, between 2009 and 2014. Within a narrow window of Hb values around the predetermined cutoff, the rate of LHD jumps discontinuously. This discontinuous jump allows us to quantify the causal effects of LHD on donor return, as it is uncorrelated with other unobserved factors that may also affect donor return.</p><p><strong>Results: </strong>We found different behavioral reactions to LHD for female and male donors. Female donors do not react to the first LHD. However, after any repeated LHD, they are 13.53 percentage points (<i>p <</i>0.001) less likely to make at least 1 donation attempt within the next 18 months and make 0.389 fewer donation attempts (<i>p <</i>0.001). Male donors react to the first LHD. They are 5.32 percentage points (<i>p</i> = 0.139) less likely to make at least 1 donation attempt over the next 18 months and make 0.227 (<i>p</i> = 0.018) fewer donation attempts. After any repeated LHD, male donors are 13.30 percentage points (<i>p</i> = 0.004) less likely to make at least 1 donation attempt and make 0.152 (<i>p</i> = 0.308) fewer donation attempts.</p><p><strong>Conclusion: </strong>LHD have detrimental impacts on donor return, especially if they occur repeatedly - suggesting that avoiding false LHD and helping donors to better cope with them helps to maintain the pool of prospective donors.</p>","PeriodicalId":520820,"journal":{"name":"Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie","volume":" ","pages":"119-128"},"PeriodicalIF":2.2,"publicationDate":"2020-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000500679","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37889916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Defining Blood Group Gene Reference Alleles by Long-Read Sequencing: Proof of Concept in the <i>ACKR1</i> Gene Encoding the Duffy Antigens.","authors":"Yann Fichou,&nbsp;Isabelle Berlivet,&nbsp;Gaëlle Richard,&nbsp;Christophe Tournamille,&nbsp;Lilian Castilho,&nbsp;Claude Férec","doi":"10.1159/000504584","DOIUrl":"https://doi.org/10.1159/000504584","url":null,"abstract":"<p><strong>Background: </strong>In the novel era of blood group genomics, (re-)defining reference gene/allele sequences of blood group genes has become an important goal to achieve, both for diagnostic and research purposes. As novel potent sequencing technologies are available, we thought to investigate the variability encountered in the three most common alleles of <i>ACKR1</i>, the gene encoding the clinically relevant Duffy antigens, at the haplotype level by a long-read sequencing approach.</p><p><strong>Materials and methods: </strong>After long-range PCR amplification spanning the whole <i>ACKR1</i> gene locus (∼2.5 kilobases), amplicons generated from 81 samples with known genotypes were sequenced in a single read by using the Pacific Biosciences (PacBio) single molecule, real-time (SMRT) sequencing technology.</p><p><strong>Results: </strong>High-quality sequencing reads were obtained for the 162 alleles (accuracy >0.999). Twenty-two nucleotide variations reported in databases were identified, defining 19 haplotypes: four, eight, and seven haplotypes in 46 <i>ACKR1</i>*<i>01</i>, 63 <i>ACKR1</i>*<i>02</i>, and 53 <i>ACKR1</i>*<i>02N.01</i> alleles, respectively.</p><p><strong>Discussion: </strong>Overall, we have defined a subset of reference alleles by third-generation (long-read) sequencing. This technology, which provides a \"longitudinal\" overview of the loci of interest (several thousand base pairs) and is complementary to the second-generation (short-read) next-generation sequencing technology, is of critical interest for resolving novel, rare, and null alleles.</p>","PeriodicalId":520820,"journal":{"name":"Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie","volume":" ","pages":"23-32"},"PeriodicalIF":2.2,"publicationDate":"2020-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000504584","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37687994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High-Throughput Screening of Blood Donors for Twelve Human Platelet Antigen Systems Using Next-Generation Sequencing Reveals Detection of Rare Polymorphisms and Two Novel Protein-Changing Variants.","authors":"Stephanie Maria Vorholt,&nbsp;Nele Hamker,&nbsp;Hagen Sparka,&nbsp;Jürgen Enczmann,&nbsp;Thomas Zeiler,&nbsp;Tanja Reimer,&nbsp;Johannes Fischer,&nbsp;Vera Balz","doi":"10.1159/000504894","DOIUrl":"https://doi.org/10.1159/000504894","url":null,"abstract":"<p><strong>Background: </strong>Exposure to non-matching human platelet alloantigens (HPA) may result in alloimmunization. Antibodies to HPA can be responsible for post-transfusion purpura, refractoriness to donor platelets, and fetal and neonatal alloimmune thrombocytopenia. For the supply of compatible apheresis platelet concentrates, the HPA genotypes are determined in a routine manner.</p><p><strong>Methods: </strong>Here, we describe a novel method for genotyping twelve different HPA systems simultaneously, including HPA-1 to HPA-5, HPA-9w, HPA-10w, HPA-16w, HPA-19w, HPA-27w, and the novel HPA-34w by means of amplicon-based next-generation sequencing (NGS). Blood donor samples of 757 individuals with a migration background and 547 of Western European ancestry were genotyped in a mass-screening setup. An in-house software was developed for fast and automatic analysis. TaqMan assay and Sanger sequencing results served for validation of the NGS workflow. Finally, blood donors were divided in several groups based on their country of origin and the allele frequencies were compared.</p><p><strong>Results: </strong>For 1,299 of 1,304 samples (99.6%) NGS was successfully performed. The concordance with TaqMan assay and Sanger sequencing results was 99.8%. Allele-calling dropouts that were observed for two samples with the TaqMan assay caused by rare single nucleotide polymorphisms were resolved by NGS. Additionally, twenty rare and two novel variants in the coding regions of the genes <i>ITGB3</i>, <i>GPB1A</i>, <i>ITGBA2</i>, and <i>CD109</i> were detected. The determined allele frequencies were similar to those published in the gnomAD database.</p><p><strong>Conclusions: </strong>No significant differences were observed in the distribution of allele frequencies of HPA-1 through HPA-5 and HPA-15 throughout the analyzed groups except for a lower allele frequency for the HPA-1b allele in the group of donors with Southern Asian ancestry. In contrast, other nucleotide variants that have not yet been phenotypically characterized occurred three times more often in blood donors with a migration background. High-throughput amplicon-based NGS is a reliable method for screening HPA genotypes in a large sample cohort simultaneously. It is easily upgradeable for genotyping additional targets without changing the setup or the analysis pipeline. Mass-screening methods will help building up blood donor registries to provide matched blood products.</p>","PeriodicalId":520820,"journal":{"name":"Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie","volume":" ","pages":"33-44"},"PeriodicalIF":2.2,"publicationDate":"2020-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000504894","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37687995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive Molecular Analysis of Serologically D-Negative and Weak/Partial D Phenotype in Thai Blood Donors.","authors":"Jairak Thongbut,&nbsp;Loann Raud,&nbsp;Claude Férec,&nbsp;Charuporn Promwong,&nbsp;Pornlada Nuchnoi,&nbsp;Yann Fichou","doi":"10.1159/000499087","DOIUrl":"https://doi.org/10.1159/000499087","url":null,"abstract":"<p><strong>Background: </strong>Molecular genetics of the Rh system has been extensively studied in Caucasians, Black Africans, East Asians, and Indians more recently. In this work, we sought to investigate the molecular basis of variant D expression in the Thai population, which remains unknown.</p><p><strong>Materials and methods: </strong>Blood samples from 450 Thai donors showing the variant D phenotype were collected. The <i>RHD</i> gene was analyzed by quantitative multiplex polymerase chain reaction of short fluorescent fragments and/or Sanger sequencing.</p><p><strong>Results: </strong>The most frequent alleles in 200 D-negative and 121 DEL samples were the whole <i>RHD</i> gene deletion and the Asian <i>DEL</i> alleles, respectively. In 129 weak/partial D samples, 36 variant alleles were identified, including eight novel alleles. <i>RHD</i>*<i>06.03</i>, which is common in variant D samples from South China, is the most prevalent variant allele, followed by the recently reported Indian <i>RHD</i>*<i>01W.150</i> allele.</p><p><strong>Discussion: </strong>For the first time, a comprehensive overview of the nature and distribution of variant <i>RHD</i> alleles in Thailand is reported. It is a milestone to pave the way towards improvement of the current screening strategy to identify DEL donors accurately. The next step will be the design and implementation of a simple molecular test for screening the most frequent alleles, specifically in this population.</p>","PeriodicalId":520820,"journal":{"name":"Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie","volume":" ","pages":"54-60"},"PeriodicalIF":2.2,"publicationDate":"2020-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000499087","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37687997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation for Perioperative Blood Transfusion during Major Abdominal Procedures in a Pediatric Population: A Retrospective Observation Cohort Study.","authors":"Hong Zhou,&nbsp;Jiaming Lan,&nbsp;Hai Zhu,&nbsp;Xingqin Tan,&nbsp;Jianxia Liu,&nbsp;Li Xiang,&nbsp;Chunbao Guo","doi":"10.1159/000497826","DOIUrl":"https://doi.org/10.1159/000497826","url":null,"abstract":"<p><strong>Objectives: </strong>The purpose of this study was to examine modifiable factors and their impact on perioperative blood transfusion for pediatric patients with major abdominal procedures.</p><p><strong>Methods: </strong>This is a retrospective review of 1,506 patients who underwent major abdominal surgical procedures in a tertiary medical center from January 2008 to June 2018. Clinical data about blood administration including triggers and targets for intra- or postoperative transfusion were collected and analyzed. The inappropriate transfusion (transfusion > 8.0 g/dL of hemoglobin [Hb] trigger) and overtrans-fusion criteria (target transfusion > 10.0 g/dL or > 2 g/dL of target minus trigger level) were applied to examine the intraoperative factors with the intraoperative transfusion practice. Perioperative morbidity was further assessed based on the inappropriate transfusion and overtransfusion status.</p><p><strong>Results: </strong>Intraoperative transfusion was used in 468 (31.1%) of the 1,506 patients included in the study. Among them, 212 (45.3%) intraoperative transfusion episodes were classified as inappropriate, and 135 cases (28.8%) were confirmed as overtransfusion. On univariate analysis, inappropriate transfusions were observed more commonly among patients with younger age (<i>p</i> < 0.001) and who underwent hepatic resection (<i>p</i> < 0.001) or intestinal resection (<i>p</i> < 0.001). Overtransfusion was also associated with elevated trigger of 8.0 g/dL Hb (<i>p</i> = 0.006) and younger age (<i>p</i> = 0.003). No perioperative complications were associated with inappropriate transfusions and overtransfusion under multivariate analysis.</p><p><strong>Conclusions: </strong>Overtransfusion was common in hepatic resection and younger age, but to definitely prove this hypothesis, a prospective randomized trial needs to be performed.</p>","PeriodicalId":520820,"journal":{"name":"Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie","volume":" ","pages":"68-74"},"PeriodicalIF":2.2,"publicationDate":"2020-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000497826","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37687999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potential of Next-Generation Sequencing in Noninvasive Fetal Molecular Blood Group Genotyping.","authors":"Sandra Wienzek-Lischka,&nbsp;Sandy Bachmann,&nbsp;Vanessa Froehner,&nbsp;Gregor Bein","doi":"10.1159/000505161","DOIUrl":"https://doi.org/10.1159/000505161","url":null,"abstract":"<p><p>Hemolytic disease of the fetus and newborn and fetal and neonatal alloimmune thrombocytopenia are caused by maternal antibodies against fetal alloantigens on red blood cells or platelets that are inherited from the father. After transplacental transport to the fetal circulation, antibodies of the IgG class may cause severe fetal anemia or bleeding complications. The indication for noninvasive fetal blood group genotyping is given if a clinically relevant antibody is detected in a pregnant woman and if the father is heterozygous (or unknown) for the implicated blood group allele. This mini-review will focus on the advantages and current limitations of next-generation sequencing (NGS) for noninvasive diagnosis of fetal blood groups which is, in contrast to fetal aneuploidy screening, proposed only by some research groups. Targeted massively parallel sequencing of short DNA fragments from maternal cell-free plasma samples enables counting of fetal alleles for many single nucleotide polymorphisms in parallel. This information can be utilized for estimation of the fetal fraction of cell-free DNA (cfDNA) as well as detection of the paternal blood group allele in question. Adherence to a cut-off of ≥4% fetal fraction for reporting conclusive results is recommended to avoid false-negative results due to low fetal fraction. For screening purposes of fetal <i>RHD</i> in RhD-negative pregnant women, real-time PCR methods are very well established. However, for diagnostic purposes, the targeted amplicon-based NGS approach has the inherent capability to estimate the fetal fraction of cfDNA. In the future, improving the accuracy of NGS by consensus sequencing of single cfDNA molecules may enable reliable fetal blood group genotyping already in the first trimester of pregnancy.</p>","PeriodicalId":520820,"journal":{"name":"Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie","volume":" ","pages":"14-22"},"PeriodicalIF":2.2,"publicationDate":"2020-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000505161","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37687993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Next-Generation Sequencing Technologies in Blood Group Typing.","authors":"Daniel Fürst,&nbsp;Chrysanthi Tsamadou,&nbsp;Christine Neuchel,&nbsp;Hubert Schrezenmeier,&nbsp;Joannis Mytilineos,&nbsp;Christof Weinstock","doi":"10.1159/000504765","DOIUrl":"https://doi.org/10.1159/000504765","url":null,"abstract":"<p><p>Sequencing of the human genome has led to the definition of the genes for most of the relevant blood group systems, and the polymorphisms responsible for most of the clinically relevant blood group antigens are characterized. Molecular blood group typing is used in situations where erythrocytes are not available or where serological testing was inconclusive or not possible due to the lack of antisera. Also, molecular testing may be more cost-effective in certain situations. Molecular typing approaches are mostly based on either PCR with specific primers, DNA hybridization, or DNA sequencing. Particularly the transition of sequencing techniques from Sanger-based sequencing to next-generation sequencing (NGS) technologies has led to exciting new possibilities in blood group genotyping. We describe briefly the currently available NGS platforms and their specifications, depict the genetic background of blood group polymorphisms, and discuss applications for NGS approaches in immunohematology. As an example, we delineate a protocol for large-scale donor blood group screening established and in use at our institution. Furthermore, we discuss technical challenges and limitations as well as the prospect for future developments, including long-read sequencing technologies.</p>","PeriodicalId":520820,"journal":{"name":"Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie","volume":" ","pages":"4-13"},"PeriodicalIF":2.2,"publicationDate":"2020-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000504765","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37687992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recommendations Regarding Practical DEL Typing Strategies for Serologically D-Negative Asian Donors.","authors":"Tae Yeul Kim,&nbsp;Yun Ji Hong,&nbsp;Mi Jung Kim,&nbsp;Hyungsuk Kim,&nbsp;Taek Soo Kim,&nbsp;Jeong Su Park,&nbsp;Kyoung Un Park,&nbsp;Kyou-Sup Han","doi":"10.1159/000500098","DOIUrl":"https://doi.org/10.1159/000500098","url":null,"abstract":"<p><strong>Background: </strong>DEL, the weakest D variant, is mistyped as D-negative by routine serological assays. Transfusion of red blood cells expressing the DEL phenotype has the potential to elicit anti-D alloimmunization in D-negative recipients. The goal of this study was to recommend DEL typing strategies for serologically D-negative Asian donors.</p><p><strong>Methods: </strong>RhCE phenotyping and the adsorption-elution test were performed on 674 serologically D-negative samples. <i>RHD</i> genotyping using real-time polymerase chain reaction and melting curve analysis were also undertaken to identify DEL alleles. Costs and turnaround time of RhCE phenotyping, the adsorption-elution test, and <i>RHD</i> genotyping were estimated.</p><p><strong>Results: </strong>Sensitivity and specificity of the adsorption-elution test for serologically D-negative samples were 94.9% (93/98) and 91.5% (527/576), respectively. C+ phenotypes were detected in all 98 samples with DEL alleles. Despite comparable costs, <i>RHD</i> genotyping was more accurate and rapid than the adsorption-elution test.</p><p><strong>Conclusions: </strong>Two practical DEL typing strategies using RhCE phenotyping as an initial screening method were recommended for serologically D-negative Asian donors. Compared with DEL typing using <i>RHD</i> genotyping, serological DEL typing using adsorption-elution test is predicted to increase the incidence of anti-D alloimmunization and decrease the D-negative donor pool without having any cost-competitiveness but can be used in laboratories where molecular methods are not applicable.</p>","PeriodicalId":520820,"journal":{"name":"Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie","volume":" ","pages":"88-93"},"PeriodicalIF":2.2,"publicationDate":"2020-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000500098","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37687432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"To Win the Battle, First Know Your Enemy: Error Rates in Immunohematology External Quality Assessment Results.","authors":"Christoph Buchta,&nbsp;Wim Coucke,&nbsp;Wolfgang R Mayr,&nbsp;Mathias M Müller,&nbsp;Günther F Körmöczi","doi":"10.1159/000499941","DOIUrl":"https://doi.org/10.1159/000499941","url":null,"abstract":"<p><strong>Background: </strong>As some errors in pretransfusion testing remain unrecognized, error rates and the resulting need for corrective measures are probably underestimated. External quality assessment (EQA) schemes could provide valuable input for identifying error-prone laboratory tests because they are designed to monitor test performance and errors. So far, however, there are only limited published data on error rates in such schemes.</p><p><strong>Methods: </strong>The types and incidence of incorrect results in an EQA scheme for red cell immunohematology with 187 participating laboratories were examined. The results of 58 distributions between 1999 and 2017 were evaluated, considering also the employed determination methods.</p><p><strong>Results: </strong>Out of a total of 58,726 results, 563 (0.96%) were incorrect. Error rates were 5.45% for antibody identification, 1.39% for Rh phenotyping, 0.83% for serologic cross-match, 0.60% for direct antiglobulin test, 0.20% for Kell phenotyping, 0.16% for antibody screening, and 0.14% for ABO phenotyping. During the observation period, 53 participants reported error-free results, while 37 reported one incorrect result and 97 repeatedly reported incorrect results for one or more analytes. Error rates obtained by manual methods significantly surpassed those obtained by automated methods (1.04 vs. 0.42%). The introduction of double testing with two different systems reduced error rates in Rh phenotyping from 1.55 to 0.50%.</p><p><strong>Conclusion: </strong>Risk assessment should consider that error rates in pretransfusion test results vary. These data delineate the error risk potential of individual laboratory tests and thus should aid in tailoring appropriate improvement measures.</p>","PeriodicalId":520820,"journal":{"name":"Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie","volume":" ","pages":"80-87"},"PeriodicalIF":2.2,"publicationDate":"2020-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000499941","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37687431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of Regular Whole-Blood Donation on Body Iron Stores.","authors":"Vijayram Reddy K,&nbsp;Shamee Shastry,&nbsp;Manish Raturi,&nbsp;Poornima Baliga B","doi":"10.1159/000499768","DOIUrl":"https://doi.org/10.1159/000499768","url":null,"abstract":"<p><strong>Background and objectives: </strong>Regular and repeat blood donations may cause reduced body iron stores (BIS), which may lead to a shrinking donor pool. Furthermore, hemoglobin (Hb) itself is quite an inadequate measure to detect iron deficiency. Hence, our primary aim was to understand the effect of repeated blood donations on BIS in regular blood donors.</p><p><strong>Study design and setting: </strong>We conducted a prospective study on voluntary whole-blood donors (September 2015 through August 2017). Donor demographics were noted. Donors were further divided into four groups, depending on their number of donations. Their samples were tested for Hb, red cell indices, and serum ferritin to check for reduced BIS. The data obtained were analyzed using SPSS version 20 (IBM, USA).</p><p><strong>Results: </strong>A total of 374 regular voluntary blood donors were included in the study. The mean serum ferritin levels in males and females were 75.4 and 36.2µg/L, respectively. There was a 45% decrement in the mean serum ferritin values in Group I (101.57 µg/L) in contrast to Group III (56.69 µg/L) (<i>p</i> < 0.0032). In the study, 9.8, 11.2, and 4.8% of the donors were in Stage 1, Stage 2, and Stage 3 of reduced BIS, respectively. Of the donors, 11.2% were in Stage 2 of reduced BIS. Further donations in such cases can compromise donor safety.</p><p><strong>Conclusion: </strong>The present study demonstrates iron depletion in regular voluntary blood donors. In 11.2% of the donors, iron deficiency erythropoiesis was noted. A ferritin screening after the first donation followed up at the tenth donation might help detect iron-deficient individuals. Iron supplementation for all regular blood donors and female donors in particular will help prevent the shrinking donor pool due to iron deficiency.</p>","PeriodicalId":520820,"journal":{"name":"Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie","volume":" ","pages":"75-79"},"PeriodicalIF":2.2,"publicationDate":"2020-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000499768","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37687430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}