Biomedical research (Tokyo, Japan)最新文献

A novel 3D immuno-electron microscopy and its application to the Golgi apparatus. 一种新型三维免疫电子显微镜及其在高尔基体上的应用。

Biomedical research (Tokyo, Japan) Pub Date : 2026-01-01 DOI: 10.2220/biomedres.47.25

Daisuke Koga, Satoshi Kusumi, Hirokazu Yagi, Koichi Kato

{"title":"A novel 3D immuno-electron microscopy and its application to the Golgi apparatus.","authors":"Daisuke Koga, Satoshi Kusumi, Hirokazu Yagi, Koichi Kato","doi":"10.2220/biomedres.47.25","DOIUrl":"https://doi.org/10.2220/biomedres.47.25","url":null,"abstract":"Serial section scanning electron microscopy (SEM) is useful for revealing the three-dimensional (3D) architecture of organelles by acquiring backscattered electron images of ultrathin serial sections of resin-embedded tissues on solid substrates. However, comprehensive analyses of organelle function require a combination of ultrastructural and molecular localization data. In the present study, we developed a novel 3D immuno-electron microscopy (immuno-EM) approach that combines Tokuyasu cryosectioning with serial section SEM to elucidate the spatial distribution of organelle-associated proteins. Thick cryosections of tissues were immunolabeled with primary antibodies and FluoroNanogold-conjugated secondary antibodies, followed by gold enhancement, resin embedding, and serial sectioning and SEM. Serial tomographic images of organelles were aligned and segmented to generate 3D reconstructions. To demonstrate the effectiveness of the method, we visualized the localization of GM130, a representative cis-Golgi matrix protein, in a 3D model of the Golgi apparatus in rat pituitary gonadotropes. The 3D model revealed a spherical Golgi apparatus composed of five cisternae arranged in cis-trans order, with GM130 localized on the outer cisternae, consistent with previous findings. Our 3D immuno-EM technique enables the detailed 3D visualization of the Golgi apparatus and other organelles as well as analyses of the spatial distribution of target proteins in their 3D reconstructions.","PeriodicalId":520570,"journal":{"name":"Biomedical research (Tokyo, Japan)","volume":"47 1","pages":"25-34"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146101229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Three-dimensional morphological analysis of ghrelin-producing cells in the rat fundic gland by combining serial section scanning electron microscopy and immunogold technique. 结合连续切片扫描电镜和免疫金技术对大鼠基底腺生长素产生细胞的三维形态学分析。

Biomedical research (Tokyo, Japan) Pub Date : 2026-01-01 DOI: 10.2220/biomedres.47.35

Daisuke Koga, Satoshi Kusumi, Takahiro Sato

{"title":"Three-dimensional morphological analysis of ghrelin-producing cells in the rat fundic gland by combining serial section scanning electron microscopy and immunogold technique.","authors":"Daisuke Koga, Satoshi Kusumi, Takahiro Sato","doi":"10.2220/biomedres.47.35","DOIUrl":"https://doi.org/10.2220/biomedres.47.35","url":null,"abstract":"Ghrelin-producing cells (ghrelin cells) in rat fundic glands were analyzed three-dimensionally by combining serial section scanning electron microscopy with immunogold labeling to elucidate their ultrastructural characteristics. This approach enabled unambiguous identification of ghrelin cells and the three-dimensional (3D) reconstruction of their organelles with special reference to primary cilium. Rat ghrelin cells were morphologically classified into two types: ciliated ghrelin cells possessing primary cilia and non-ciliated ghrelin cells. In ciliated cells, primary cilia protruded from basal bodies located near the Golgi apparatus and were largely or entirely enclosed within ciliary pockets. Ghrelin-positive secretory granules were electron-dense and spherical. Ciliated and non-ciliated cells contained both large (250-350 nm) and small (100-200 nm) ghrelin-positive granules. In ciliated cells, the granules were densely accumulated in a localized region of the cytoplasm, being opposite the Golgi apparatus across the nucleus; in non-ciliated cells they were densely distributed throughout the cytoplasm. The ultrastructure of the basement membrane and overall 3D configuration of the Golgi apparatus also differed between the two cell types. Our results provide novel insights into the morphological organization of ghrelin cells, whose 3D ultrastructural features have been unclear in conventional two-dimensional transmission electron microscopy using single ultrathin sections.","PeriodicalId":520570,"journal":{"name":"Biomedical research (Tokyo, Japan)","volume":"47 1","pages":"35-46"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146101205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differences in tumor-associated cytokine production and immune mechanisms between myofibroblastic cancer associated fibroblasts and myofibroblasts. 肌成纤维细胞癌相关成纤维细胞和肌成纤维细胞之间肿瘤相关细胞因子产生和免疫机制的差异。

Biomedical research (Tokyo, Japan) Pub Date : 2026-01-01 DOI: 10.2220/biomedres.47.67

Kosei Kubota, Ken Furudate, Tomoh Matsumiya, Norihiko Narita, Yoshihiro Tamura, Eiji Ikami, Wataru Kobayashi

{"title":"Differences in tumor-associated cytokine production and immune mechanisms between myofibroblastic cancer associated fibroblasts and myofibroblasts.","authors":"Kosei Kubota, Ken Furudate, Tomoh Matsumiya, Norihiko Narita, Yoshihiro Tamura, Eiji Ikami, Wataru Kobayashi","doi":"10.2220/biomedres.47.67","DOIUrl":"https://doi.org/10.2220/biomedres.47.67","url":null,"abstract":"Despite advances in oral cancer treatment, therapeutic resistance remains a major challenge. Cancer-associated fibroblasts (CAFs) play a pivotal role in shaping the tumor microenvironment. Building on our previous findings on immune responses in gingival fibroblasts, we investigated the immune characteristics of CAFs. Myofibroblast CAF-like cells (myCAF-like) were established using a co-culture system and compared with gingival fibroblast-derived myofibroblasts. MyCAF-like cells exhibited markedly reduced expression of TLR3 and TLR4, whereas RIG-I, COX-2, IL-1β, IL-6, and IL-8 were significantly upregulated. Activation of p38 MAPK signaling contributed to the increased expression of COX-2 and pro-inflammatory cytokines, and TGF-β was involved in the phenotypic transformation of gingival fibroblasts into myCAF-like cells. Microarray analysis revealed upregulation of IL-1α, IL-1β, CXCL8, PTGS2, and CXCL5, alongside downregulation of OMD, COL15A1, and ASPN in myCAF-like cells. Collectively, these findings demonstrate that CAFs acquire a distinct inflammatory signature that differs from gingival fibroblasts and promotes tumor invasion and progression. Targeting CAF-associated inflammatory and signaling pathways may represent a promising strategy to overcome tumor infiltration and treatment resistance in oral cancer.","PeriodicalId":520570,"journal":{"name":"Biomedical research (Tokyo, Japan)","volume":"47 2","pages":"67-76"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147518001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tenascin-XB plays a role in the infiltration of immune cells in tumor microenvironment. Tenascin-XB在肿瘤微环境中参与免疫细胞的浸润。

Biomedical research (Tokyo, Japan) Pub Date : 2026-01-01 DOI: 10.2220/biomedres.47.11

Ao Gong, Yuichi Iida, Kohei Kawakami, Kazuo Yamada, Yasuyuki Saito, Ken-Ichi Matsumoto

{"title":"Tenascin-XB plays a role in the infiltration of immune cells in tumor microenvironment.","authors":"Ao Gong, Yuichi Iida, Kohei Kawakami, Kazuo Yamada, Yasuyuki Saito, Ken-Ichi Matsumoto","doi":"10.2220/biomedres.47.11","DOIUrl":"https://doi.org/10.2220/biomedres.47.11","url":null,"abstract":"We previously showed that tenascin-XB (TNXB) contributes to tumor suppressor function. The present study aimed to assess the tumor-suppressive mechanism of TNXB by focusing on immune cell infiltration into the tumor microenvironment (TME). We revealed that B16-OVA melanoma cells (MO5)-bearing TNXB-deficient (Tnxb-/-) mice exhibited significant tumor progression and a poor survival rate. Allogeneic mixed lymphocyte reaction showed reduced numbers and increased activation of both CD4+ and CD8+ T cells from Tnxb-/- spleens. Moreover, T cell activation assay further proved that CD4+ and CD8+ T cells from Tnxb-/- mice were more activated than those from WT mice. RT-qPCR analysis showed that expression of T cell activation-related cytokines and chemokines was significantly decreased in tumor tissues from Tnxb-/- mice. Flow cytometry analysis revealed a reduced infiltration level of CD8+ T cells in both naïve spleens and tumor tissues in Tnxb-/- mice. Ultimately, total activation of CD8+ T cells was decreased in tumor tissues in Tnxb-/- mice. In conclusion, we found that although Tnxb-/- CD4+ and CD8+ T cells tend to be activated more than WT CD4+ and CD8+ T cells, CD8+ T cell infiltration and activation level were attenuated in tumor sites of Tnxb-/- mice.","PeriodicalId":520570,"journal":{"name":"Biomedical research (Tokyo, Japan)","volume":"47 1","pages":"11-23"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146101268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ultrastructural spectrum of oncocytic and chromophobe renal tumors: Insights from osmium maceration-based scanning electron microscopy of formalin-fixed tissues. 嗜癌性和憎色性肾肿瘤的超微结构光谱：福尔马林固定组织基于锇浸渍扫描电镜的观察。

Biomedical research (Tokyo, Japan) Pub Date : 2026-01-01 DOI: 10.2220/biomedres.47.77

Taro Murakami, Daisuke Koga, Mayuko Akimoto, Hirohisa Okushima, Masayo Kamikokura, Akinori Tada, Kumi Takasawa, Masanori Goto, Masaki Murata, Makoto Osanai, Yoji Nagashima, Yasunari Takakuwa, Akira Takasawa

{"title":"Ultrastructural spectrum of oncocytic and chromophobe renal tumors: Insights from osmium maceration-based scanning electron microscopy of formalin-fixed tissues.","authors":"Taro Murakami, Daisuke Koga, Mayuko Akimoto, Hirohisa Okushima, Masayo Kamikokura, Akinori Tada, Kumi Takasawa, Masanori Goto, Masaki Murata, Makoto Osanai, Yoji Nagashima, Yasunari Takakuwa, Akira Takasawa","doi":"10.2220/biomedres.47.77","DOIUrl":"https://doi.org/10.2220/biomedres.47.77","url":null,"abstract":"Ultrastructural analysis has declined in diagnostic pathology since the advent of immunohistochemistry for formalin-fixed paraffin-embedded tissues. To reevaluate the utility of ultrastructural analysis, we adopted the osmium maceration method for scanning electron microscopy (SEM) of formalin-fixed surgical specimens: renal oncocytoma, chromophobe renal cell carcinoma (ChRCC), and tumors categorized as other oncocytic tumors of the kidney (OOT). Light microscopy and immunohistochemistry have revealed that these tumors have overlapping features. SEM observation of the formalin-fixed tumors highlighted distinct morphological features: The oncocytomas contained large mitochondria with vesicular cristae; ChRCCs harbored small mitochondria with microvesicles, and OOTs showed abundant but small mitochondria with lamellar cristae. Quantitative morphometry confirmed that the number of mitochondria in oncocytomas and OOTs exceeded that of non-neoplastic lesions, whereas ChRCCs contained fewer mitochondria. In addition, mitochondria in ChRCCs and OOTs were smaller than those of non-neoplastic lesions, whereas mitochondria in oncocytomas were larger. Thus, SEM of formalin-fixed tissues, optimized by osmium maceration, can provide diagnostically valuable information beyond conventional histology and immunohistochemistry that may help to clarify the biology of challenging oncocytic and chromophobe renal tumors.","PeriodicalId":520570,"journal":{"name":"Biomedical research (Tokyo, Japan)","volume":"47 2","pages":"77-87"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147518017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Perfluorohexanesulfonic acid-induced inhibition of human palate cell proliferation through upregulation of miR-374a-5p. 全氟己磺酸通过上调miR-374a-5p诱导抑制人腭细胞增殖。

Biomedical research (Tokyo, Japan) Pub Date : 2026-01-01 DOI: 10.2220/biomedres.47.1

Hiroki Yoshioka, Hanane Horita, Kenichi Ogata, Kazuki Takeda, Hyogo Horiguchi, Yosuke Tsukiboshi

{"title":"Perfluorohexanesulfonic acid-induced inhibition of human palate cell proliferation through upregulation of miR-374a-5p.","authors":"Hiroki Yoshioka, Hanane Horita, Kenichi Ogata, Kazuki Takeda, Hyogo Horiguchi, Yosuke Tsukiboshi","doi":"10.2220/biomedres.47.1","DOIUrl":"https://doi.org/10.2220/biomedres.47.1","url":null,"abstract":"Per- and poly fluoroalkyl substances (PFAS) pose significant global health risks. Although the use of classical PFAS such as perfluorooctane sulfonic acid (PFOS) and perfluorooctanoic acid (PFOA) is regulated, the toxicological effects of alternative PFASs remain unknown. Cleft palate is a congenital condition influenced by both environmental and genetic factors. Although PFOS has been linked to cleft palate, the effects of other PFAS compounds remain unexplored. The aim of this study was to clarify the involvement of classical and alternative PFAS (PFHxA and PFHxS) in human embryonic palatal mesenchymal cell (HEPM) proliferation. Following PFAS treatment for 48 h, cell viability, apoptosis, and expression of cell cycle-related proteins were tested. In addition, miRNA levels and predicted target genes were measured, and a rescue experiment against PFHxS was conducted using an miR-374a-5p inhibitor. Among the four PFASs, PFHxS decreased the number of cells showing cyclin- and cyclin-dependent kinase reduction. In addition, PFHxS treatment upregulated miR-374a-5p and downregulated its downstream genes. Furthermore, miR- 374a-5p inhibitor alleviated the PFHxS-induced reduction in cell proliferation. These findings therefore indicate that miR-374a-5p plays a key role in the development of PFHxS-induced cleft palate and that alternative PFAS may have a highly toxic effect on HEPM cells.","PeriodicalId":520570,"journal":{"name":"Biomedical research (Tokyo, Japan)","volume":"47 1","pages":"1-10"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146101266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spatiotemporal dynamics of exercise-induced macrophages influences axonal regeneration after peripheral nerve injury. 运动诱导巨噬细胞的时空动态影响周围神经损伤后轴突再生。

Biomedical research (Tokyo, Japan) Pub Date : 2026-01-01 DOI: 10.2220/biomedres.47.55

Kyosuke Fukuda, Yuki Minegishi, Michiaki Sato, Yuta Sakamoto, Satoshi Shimo, Takashi Amari, Kenji Murata, Naohiko Kanemura

{"title":"Spatiotemporal dynamics of exercise-induced macrophages influences axonal regeneration after peripheral nerve injury.","authors":"Kyosuke Fukuda, Yuki Minegishi, Michiaki Sato, Yuta Sakamoto, Satoshi Shimo, Takashi Amari, Kenji Murata, Naohiko Kanemura","doi":"10.2220/biomedres.47.55","DOIUrl":"https://doi.org/10.2220/biomedres.47.55","url":null,"abstract":"This study aimed to elucidate the relationship between the spatiotemporal behavior of macrophages during exercise and axonal regeneration following peripheral nerve injury. A sciatic nerve crush model was created using male C57BL/6J mice, which were divided into sedentary and exercise groups. The exercise group performed low-intensity treadmill running (10 m/min, 60 min/day, 5 days/week) starting 3 days post-injury. Immunohistochemical analyses of Neurofilament 200 kDa (NF200), Growth Associated Protein-43 (GAP-43), F4/80, and arginase-1 (Arg-1), a representative marker of M2 macrophages and a downstream effector of the JAK-STAT6 signaling pathway, were conducted in the proximal and distal regions of the injured nerves. Functional recovery was assessed using the sciatic functional index (SFI) and compound muscle action potential (CMAP). At 7 and 14 days post-injury, the exercised group exhibited a significant increase in axonal number and Arg-1-positive area, specifically in the distal region. SFI and CMAP analyses also demonstrated enhanced functional recovery in the exercise group. These findings suggest that low-intensity treadmill exercise may enhance axonal regeneration, potentially through transient activation and local accumulation of M2-polarized macrophages. Therefore, exercise-induced regulation of macrophage dynamics may represent a novel therapeutic strategy for peripheral nerve repair.","PeriodicalId":520570,"journal":{"name":"Biomedical research (Tokyo, Japan)","volume":"47 2","pages":"55-66"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147518027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dietary supplementation of myo-inositol for preventing fatty liver disease by altering hepatic epigenetic modifications in the transcribed regions of Fasn and Elovl6. 膳食补充肌醇通过改变Fasn和elov16转录区域的肝脏表观遗传修饰来预防脂肪肝疾病。

Biomedical research (Tokyo, Japan) Pub Date : 2026-01-01 DOI: 10.2220/biomedres.47.47

Masaya Shimada, Mayu Hibi, Saki Ozeki, Ayumu Kagiya, Tomoyuki Nakagawa

引用次数: 0

The distinct effect between Amyloid β (1‒40) and Amyloid β (1‒42) on the TRAP-stimulated platelet activation in diabetes mellitus. β淀粉样蛋白（1-40）和β淀粉样蛋白（1-42）对trap刺激的糖尿病血小板活化的不同影响。

Biomedical research (Tokyo, Japan) Pub Date : 2025-01-01 DOI: 10.2220/biomedres.46.119

Takuya Omura, Chika Usui, Rie Matsushima-Nishiwaki, Osamu Kozawa, Haruhiko Tokuda

{"title":"The distinct effect between Amyloid β (1‒40) and Amyloid β (1‒42) on the TRAP-stimulated platelet activation in diabetes mellitus.","authors":"Takuya Omura, Chika Usui, Rie Matsushima-Nishiwaki, Osamu Kozawa, Haruhiko Tokuda","doi":"10.2220/biomedres.46.119","DOIUrl":"https://doi.org/10.2220/biomedres.46.119","url":null,"abstract":"Amyloid β (Aβ) (1‒40) is the major form in amyloid plaques, while Aβ (1‒42) is predominant in neuronal plaques. Anti-Aβ antibodies are clinically accepted for Alzheimer's disease treatment to remove Aβ from neuronal plaques; however, increase of intracranial hemorrhagic risk is a major concern. We reported that Aβ (1‒40) inhibits thrombin receptor-activating protein (TRAP)-induced platelet activation in healthy volunteers, and the responsiveness of Aβ (1‒40) to the platelet activation is related to brain atrophy in diabetes mellitus (DM) patients. We investigated the difference between the effects of Aβ (1‒40) and Aβ (1‒42) on the platelet activation in DM participants. Both isoforms suppressed the platelet aggregation, but the effect of Aβ (1‒42) was smaller than Aβ (1‒40). The effect of Aβ (1‒42) on the TRAP-stimulated phosphorylation of p38 mitogen-activated protein kinase and stress-activated protein kinase/c-Jun N-terminal kinase was smaller than Aβ (1‒40). Although the differences were not clarified, the effective ratio of Aβ (1‒40) to Aβ (1‒42) on the PDGF-AB secretion effect was related to the ratio on the aggregation and the phosphorylated-HSP27 secretion. These results suggest that the difference of the effects exists between Aβ (1‒40) and Aβ (1‒42) on the TRAP-stimulated platelet activation individually in the DM patients.","PeriodicalId":520570,"journal":{"name":"Biomedical research (Tokyo, Japan)","volume":"46 3","pages":"119-128"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144176260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Age-dependent changes of the perineuronal net in the mouse basal ganglia nuclei. 小鼠基底神经节核神经元周围网的年龄依赖性变化。

Biomedical research (Tokyo, Japan) Pub Date : 2025-01-01 DOI: 10.2220/biomedres.46.155

Fuyuki Karube, Linsen Lu, Fumino Fujiyama

{"title":"Age-dependent changes of the perineuronal net in the mouse basal ganglia nuclei.","authors":"Fuyuki Karube, Linsen Lu, Fumino Fujiyama","doi":"10.2220/biomedres.46.155","DOIUrl":"https://doi.org/10.2220/biomedres.46.155","url":null,"abstract":"Aging promotes mild but progressive decline of motor behaviors and cognitive functions, related to loss of plasticity. Recovery of neural plasticity may become a possible clinical treatment against aging. Perineuronal net (PNN) is the extracellular matrix surrounding cell bodies of neurons, and is known to affect plasticity and survival of neurons. The basal ganglia are motor-related regions of the brain, and earlier reports revealed that neurons in the output nuclei of the basal ganglia form PNN. However, the detailed characteristics are not fully clarified yet. We found intensive PNN expression in the entopeduncular nucleus (EP) and substantia nigra pars reticulata (SNr). PNN surrounded parvalbumin-expressing large axon terminals in the EP and SNr. The proportion of PNN-forming neurons was lower in young mice, and gradually increased with age. The fluorescent intensity of PNN showed similar changes in the EP and SNr. We also showed that the motor ability examined by the rotarod test correlated with PNN expression in the SNr. It may provide the possibility of PNN modulation to improve plasticity and quality of behaviors in aged animals.","PeriodicalId":520570,"journal":{"name":"Biomedical research (Tokyo, Japan)","volume":"46 4","pages":"155-166"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144755617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0