Discover animalsPub Date : 2024-01-01Epub Date: 2024-11-06DOI: 10.1007/s44338-024-00031-0
Lakshminarasimhan Sundarrajan, Umar Farouk Mustapha, Suraj Unniappan
{"title":"Stress induces nucleobindin-1 mRNA and nesfatin-1-like peptide stimulates cortisol secretion in goldfish.","authors":"Lakshminarasimhan Sundarrajan, Umar Farouk Mustapha, Suraj Unniappan","doi":"10.1007/s44338-024-00031-0","DOIUrl":"10.1007/s44338-024-00031-0","url":null,"abstract":"<p><p>Stress is a state of disrupted homeostasis triggered by physical or psychological stimuli that elicit adaptive responses at the molecular and cellular levels. In fish, the hypothalamus-pituitary-interrenal (HPI) axis mediates stress responses. Nesfatin-1 and a nesfatin-1-like peptide (NLP), derived from nucleobindin-1 (NUCB1), have been implicated in stress hormone regulation in mammals. This study investigated the cell-specific expression of NUCB1/NLP in HPI tissues and its effects on stress response in goldfish <i>(Carassius auratus)</i>. NUCB1 mRNA is abundant in the hypothalamus, pituitary, and several other peripheral tissues of goldfish. NUCB1/NLP-like immunoreactivity was found in the brain and pituitary, co-localized with corticotropin-releasing factor receptor 1 (CRF-R1) in the hypothalamus, and with adrenocorticotrophic hormone (ACTH) in the pituitary. In vivo netting and restraint stress increased <i>nucb1</i> and <i>crf-r1</i> mRNAs in the brain and <i>acth</i> mRNA in the pituitary, as determined by RT-qPCR. Intraperitoneal injection of NLP increased cortisol in circulation, <i>crf-r1</i> mRNA in the brain and <i>acth</i> mRNA in the pituitary. These findings suggest that NUCB1/NLP is a new player in mediating the endocrine stress response of goldfish through the HPI axis.</p>","PeriodicalId":520282,"journal":{"name":"Discover animals","volume":"1 1","pages":"32"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11562699/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142635491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}