International journal of biochemistry & physiology最新文献

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Association of Galectin-3 but not Laminin in Tamoxifen-Induced Growth Suppression in Breast Cancer MCF-7 Cells. 半乳糖凝集素-3而非层粘连蛋白与他莫昔芬诱导的乳腺癌MCF-7细胞生长抑制的关系
International journal of biochemistry & physiology Pub Date : 2025-01-01 Epub Date: 2025-07-08 DOI: 10.23880/ijbp-16000267
A Segree, S Flemmings, S Nagdas, S Raychoudhury
{"title":"Association of Galectin-3 but not Laminin in Tamoxifen-Induced Growth Suppression in Breast Cancer MCF-7 Cells.","authors":"A Segree, S Flemmings, S Nagdas, S Raychoudhury","doi":"10.23880/ijbp-16000267","DOIUrl":"https://doi.org/10.23880/ijbp-16000267","url":null,"abstract":"<p><p>Tamoxifen (TAM), a selective estrogen receptor modulator, competes with estrogen for binding to estrogen receptor (ER) sites, thereby inhibiting estrogen-induced proliferation in ER-positive breast cancer cells. However, long-term treatment with TAM often leads to resistance, and its mechanisms, particularly involving biochemical markers, remain unclear. This study investigated the effects of TAM (10<sup>-6</sup> M and 10<sup>-8</sup> M) compared to 17β-estradiol (10<sup>-6</sup> M and 10<sup>-8</sup> M) on galectin-3 and laminin expressions in ER-positive MCF-7 breast cancer cells. The MTT assays showed that 10<sup>-6</sup> M TAM significantly reduced cell viability and altered cellular morphology. Indirect immunofluorescence microscopy revealed that galectin-3 was present on the cell surface and in the nucleus; however, its localization was diminished after 48 hours of TAM (10<sup>-6</sup> M) exposure. Western blot analysis showed no change in galectin-3 expression at 24 hours but a significant reduction at 48 and 72 hours. In contrast, laminin expression remained unchanged. ELISA results further confirmed reduced galectin-3 but not laminin levels after 48 hours of TAM treatment. These findings suggest that galectin-3, but not laminin, is involved in TAM-induced suppression of MCF-7 cell growth and may serve as a biochemical marker for TAM responsiveness.</p>","PeriodicalId":520017,"journal":{"name":"International journal of biochemistry & physiology","volume":"10 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13629615/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149122589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Sub2: A TFIIB Interacting Protein with Pleiotropic Roles in mRNA Synthesis, Processing and Transport. Sub2:在mRNA合成、加工和运输中具有多效性作用的TFIIB相互作用蛋白。
International journal of biochemistry & physiology Pub Date : 2024-01-01 Epub Date: 2024-06-06 DOI: 10.23880/ijbp-16000250
E Fidler, A Ansari
{"title":"Sub2: A TFIIB Interacting Protein with Pleiotropic Roles in mRNA Synthesis, Processing and Transport.","authors":"E Fidler, A Ansari","doi":"10.23880/ijbp-16000250","DOIUrl":"10.23880/ijbp-16000250","url":null,"abstract":"<p><p>The generally accepted view is that different steps of transcription, mRNA processing and mRNA trafficking are performed by dedicated accessory factors. The research during last couple of decades, however, has challenged this dogma. We recently showed that the general transcription factor TFIIB, in addition to its well established role in initiation, is also involved in termination of transcription. Mass spectrometry of purified TFIIB revealed Sub2 as one of its major interacting partners. Sub2 has already been implicated in elongation step of transcription, splicing and mRNA trafficking. Interaction of Sub2 with TFIIB implicates it in initiation and termination steps of transcription. Multiplicity of Sub2 biological roles may be responsible for its link with a number of human pathological conditions making it a potential drug target.</p>","PeriodicalId":520017,"journal":{"name":"International journal of biochemistry & physiology","volume":"9 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12055297/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144035170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Fibrinogen in Hamster Cauda Sperm. 仓鼠尾鳍精子中的纤维蛋白原
International journal of biochemistry & physiology Pub Date : 2023-01-01 Epub Date: 2023-10-21 DOI: 10.23880/ijbp-16000237
T Salters, B Sharpe, S S Raychoudhury, S K Nagdas
{"title":"Fibrinogen in Hamster Cauda Sperm.","authors":"T Salters, B Sharpe, S S Raychoudhury, S K Nagdas","doi":"10.23880/ijbp-16000237","DOIUrl":"10.23880/ijbp-16000237","url":null,"abstract":"<p><p>Epididymis plays a vital role in promoting sperm maturation and maintaining sperm viability. It has been shown the presence of nonviable sperm in cauda epididymis. We previously identified a secretory protein (260/280KDa oligomers) of hamster cauda epididymal principal cells that binds to nonviable sperm. The 260/280KDa oligomers are composed of 64kDa FGL2 (fibrinogen-like protein-2) and 33kDa FGL1) (fibrinogen-like protein-1). In addition, we have demonstrated that FGL2 is a phospholipid-activated serine protease; the conversion of prothrombin to thrombin by FGL2 followed by the conversion of soluble fibrinogen to insoluble fibrin polymers by thrombin. In the present study, we have shown the presence of a 56kDa fibrinogen β in hamster cauda sperm. The potential role of fibrinogen in hamster physiology is being discussed.</p>","PeriodicalId":520017,"journal":{"name":"International journal of biochemistry & physiology","volume":"8 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11323058/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141984279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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