Biophotonics discovery最新文献

{"title":"Skin Tone in Biophotonics.","authors":"Jessica Ramella-Roman, Kimani Toussaint, Muyinatu A Lediju Bell, Chetan Patil, Matthew Keller","doi":"10.1117/1.BIOS.3.2.022501","DOIUrl":"https://doi.org/10.1117/1.BIOS.3.2.022501","url":null,"abstract":"<p><p>The editorial presents an overview of the BIOS special section on Skin Tone in Biophotonics.</p>","PeriodicalId":519981,"journal":{"name":"Biophotonics discovery","volume":"3 2","pages":"022501"},"PeriodicalIF":0.0,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13559160/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148932372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Engineering transparency: a conversation with Guosong Hong.","authors":"Darren Roblyer, Travis Sawyer","doi":"10.1117/1.BIOS.3.2.020501","DOIUrl":"https://doi.org/10.1117/1.BIOS.3.2.020501","url":null,"abstract":"<p><p>BIOS Editor-in-Chief Darren Roblyer and Associate Editor Travis Sawyer interviewed Guosong Hong about his path in biophotonics.</p>","PeriodicalId":519981,"journal":{"name":"Biophotonics discovery","volume":"3 2","pages":"020501"},"PeriodicalIF":0.0,"publicationDate":"2026-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13559161/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148932366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"NIH funding in the biophotonics community.","authors":"Aarohi Mahesh Mehendale, Darren Roblyer","doi":"10.1117/1.BIOS.3.2.020601","DOIUrl":"10.1117/1.BIOS.3.2.020601","url":null,"abstract":"<p><strong>Significance: </strong>This work provides a data-driven characterization of NIH funding to the biophotonics community, offering investigators, researchers, and funding agencies a clearer picture of where the field excels and where opportunities for growth remain.</p><p><strong>Aim: </strong>We aim to characterize the current NIH funding landscape supporting core biophotonics technologies and identify strengths, gaps, and opportunities for future growth.</p><p><strong>Approach: </strong>NIH-funded biophotonics projects in 2024 were identified using two complementary strategies: (1) a broad keyword-driven search of NIH RePORTER and (2) a targeted investigator-centered search based on participation in biophotonics-focused journals and conferences. Project abstracts were analyzed using a large language model to perform keyword clustering and automated classification across multiple dimensions, including funding institute, optical modality, clinical/disease focus, and geographic distribution. A focused analysis of the National Institute of Biomedical Imaging and Bioengineering (NIBIB) portfolio was also performed.</p><p><strong>Results: </strong>The broad query captured ∼$3.3 billion in 2024 grant funding that may be connected to biophotonics. However, optical technologies were peripheral to the primary research aims in a substantial fraction of these awards. The targeted community-based approach identified $288 million in funding directly aligned with core biophotonic imaging and sensing. Cancer, neuroscience, and vision collectively represented 44% of this direct portfolio. The most common modalities included microscopy, endoscopy, fluorescence/bioluminescence imaging, and optical coherence tomography. Funding related to chronic diseases (e.g., cardiovascular and kidney disease), infectious diseases, aging, and reproductive and child health was proportionally underrepresented relative to NIH-wide priorities.</p><p><strong>Conclusions: </strong>NIH exhibits strong support for biophotonics innovation in cancer, neuroscience, and vision science, reflecting historical strengths of the field. However, a substantial opportunity exists to expand impact toward areas of high national health burden where optical technologies remain underutilized. These data may help inform NIH institute-level strategies and community advocacy efforts to align future biophotonics investments with broader public health needs.</p>","PeriodicalId":519981,"journal":{"name":"Biophotonics discovery","volume":"3 2","pages":"020601"},"PeriodicalIF":0.0,"publicationDate":"2026-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13559162/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148932396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optical phantoms for the characterization and validation of imaging and spectroscopy: a review.","authors":"Vivian C Krause, Alec B Walter, Anita Mahadevan-Jansen, E Duco Jansen","doi":"10.1117/1.BIOS.3.2.022511","DOIUrl":"10.1117/1.BIOS.3.2.022511","url":null,"abstract":"<p><strong>Significance: </strong>Tissue-mimicking optical phantoms are an essential tool in the development, characterization, and calibration of instruments used in optical imaging, spectroscopy, diagnosis, and treatment of disease.</p><p><strong>Aim: </strong>Major developments in optical phantoms have been enabled by recent advances in material development and characterization, as well as sophisticated manufacturing techniques. In this review, we will focus primarily on the developments in the field over the past 20 years since the seminal review paper on this topic by Pogue et al. in 2006. Throughout, we have attempted to provide the reader with practical guidelines to consider when producing phantoms.</p><p><strong>Approach: </strong>Here, we review and assess phantom geometries and available materials for phantom matrices, as well as absorbing and scattering agents. We then review and discuss the use of tissue phantoms in a variety of optical imaging and spectroscopic modalities and address the use and challenges of models of light propagation to extract optical properties from the measured physical parameters. Finally, we highlight the use and utility of tissue optical phantoms applied to the specific challenge of accounting for melanin when using optical systems to extract optical properties and by extension, physiological information, through skin.</p><p><strong>Results: </strong>In this paper, we provide a comprehensive review of material selection and fabrication considerations for the design of tissue optical phantoms. We then describe how tissue optical phantoms are designed and used for specific optical modalities, emphasizing both recent developments and current limitations, and making the argument for more standardization and detailed reporting of tissue phantom production.</p><p><strong>Conclusions: </strong>Throughout this review, we provide the reader with practical \"how to\" information for the production of tissue phantoms that are targeted for the optical modality and characterization goal. As innovative optical imaging and spectroscopy technologies are continually developed, rigorous testing and validation with well-designed optical phantoms are necessary.</p>","PeriodicalId":519981,"journal":{"name":"Biophotonics discovery","volume":"3 2","pages":"022511"},"PeriodicalIF":0.0,"publicationDate":"2026-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13559165/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148932398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of skin pigmentation on photoacoustic tomography: a phantom study.","authors":"Rianne F G Bulthuis, Nan S Lubbers, Felix Lucka, Wiendelt Steenbergen, Nienke Bosschaart, Srirang Manohar","doi":"10.1117/1.BIOS.3.2.022510","DOIUrl":"10.1117/1.BIOS.3.2.022510","url":null,"abstract":"<p><strong>Significance: </strong>Skin pigmentation is a known source of bias in optical and photoacoustic imaging. Although these effects are well investigated for two-dimensional photoacoustic imaging systems, their impact on three-dimensional (3D) photoacoustic tomography remains largely unexplored.</p><p><strong>Aim: </strong>We aim to generate insights into the impact of skin pigmentation on the performance of 3D breast photoacoustic tomography using phantoms.</p><p><strong>Approach: </strong>We developed hemispherical breast phantoms compatible with a multi-wavelength photoacoustic-ultrasound tomography system. The phantoms incorporate a thin skin-mimicking layer over an adipose-mimicking bulk material. Four phantoms with identical geometry and increasing pigmentation levels were fabricated. Embedded wall-less channels filled with sulfate solutions of varying concentrations enabled depth- and spectral-dependent analysis.</p><p><strong>Results: </strong>Increasing pigmentation led to stronger skin photoacoustic signals, reduced signal-to-background ratios at depth, and increased reflection-related background artifacts. Spectral measurements revealed pigmentation-dependent attenuation and reduced chromophore contrast in the wavelength range relevant for oxygen saturation estimation.</p><p><strong>Conclusions: </strong>Three-dimensional photoacoustic tomography is susceptible to pigmentation-induced bias affecting imaging depth and spectral analysis. The presented phantom provides a validated platform for developing strategies to improve the reliability and inclusivity of photoacoustic tomography imaging across skin tones.</p>","PeriodicalId":519981,"journal":{"name":"Biophotonics discovery","volume":"3 2","pages":"022510"},"PeriodicalIF":0.0,"publicationDate":"2026-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13559163/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148932387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Temperature-induced hemoglobin dynamics: oxygenation-dependent evolution of hemoglobin spectral response in the near-infrared.","authors":"Alessandro Bossi, Fabio Negretti, Paola Saccomandi, Antonio Pifferi","doi":"10.1117/1.BIOS.3.2.025004","DOIUrl":"10.1117/1.BIOS.3.2.025004","url":null,"abstract":"<p><strong>Significance: </strong>Monitoring thermally induced optical changes can guide thermal therapies, but these signals depend strongly on hemoglobin oxygenation, leading to different spectral responses under <i>ex vivo</i> and <i>in vivo</i> conditions. Clarifying these effects is essential for interpreting absorption measurements during treatment.</p><p><strong>Aim: </strong>We characterized near-infrared absorption spectra of hemoglobin during heating under controlled oxygenated and deoxygenated conditions and related the signatures to hemoglobin chemistry.</p><p><strong>Approach: </strong>Using time-domain diffuse optical spectroscopy, we measured 680- to 1110-nm absorption spectra of blood phantoms containing oxyhemoglobin (oxyHb) or deoxyhemoglobin (deoxyHb) during controlled heating. Multi-concentration measurements yielded extinction coefficients for native and thermally denatured species.</p><p><strong>Results: </strong>Heating oxyHb caused a broad absorption rise consistent with autoxidation of ferrous ( <math> <mrow> <msup><mrow><mi>Fe</mi></mrow> <mrow><mn>2</mn> <mo>+</mo></mrow> </msup> </mrow> </math> ) heme to ferric ( <math> <mrow><msup><mi>Fe</mi> <mrow><mn>3</mn> <mo>+</mo></mrow> </msup> </mrow> </math> ) forms, similar to methemoglobin and hemichromes. Heated deoxyHb showed distinct peaks at 774 and 874 nm that disappeared upon re-oxygenation, after which spectra converged to treated oxyHb. The oxygen-dependent kinetics of the 774/874 nm bands support a ferrous-denatured intermediate that converts to ferric hemichromes when oxygen becomes available.</p><p><strong>Conclusions: </strong>Thermal denaturation of hemoglobin follows distinct oxygenation-dependent spectroscopic pathways. The extracted extinction coefficients provide essential inputs for optical models of laser-based ablation and improve diffuse optical monitoring of thermal therapies.</p>","PeriodicalId":519981,"journal":{"name":"Biophotonics discovery","volume":"3 2","pages":"025004"},"PeriodicalIF":0.0,"publicationDate":"2026-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13559164/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148932410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Commercialization Report: Modulim Inc.","authors":"Amaan Mazhar, David Cuccia","doi":"10.1117/1.BIOS.3.1.010502","DOIUrl":"https://doi.org/10.1117/1.BIOS.3.1.010502","url":null,"abstract":"<p><p>This community report discusses the experience of navigating the complex path of commercializing biomedical technologies, offering lessons for the scientific community and industry stakeholders alike.</p>","PeriodicalId":519981,"journal":{"name":"Biophotonics discovery","volume":"3 1","pages":"010502"},"PeriodicalIF":0.0,"publicationDate":"2026-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13227149/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148160296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functional dual-slope frequency-domain near-infrared spectroscopy data interpreted with two- and three-layer models.","authors":"Jodee Frias, Giles Blaney, Angelo Sassaroli, Sergio Fantini","doi":"10.1117/1.BIOS.3.2.025003","DOIUrl":"10.1117/1.BIOS.3.2.025003","url":null,"abstract":"<p><strong>Significance: </strong>Functional near-infrared spectroscopy (fNIRS) is impacted by signal contamination from superficial hemodynamics. It is important to develop methods that account for such contamination and provide accurate measurements of cerebral hemodynamics.</p><p><strong>Aim: </strong>We aim to investigate whether simulated data with two- or three-layer tissue models are able to reproduce <i>in vivo</i> data collected with dual-slope (DS) frequency-domain (FD) near-infrared spectroscopy (NIRS) on human subjects during brain activation.</p><p><strong>Approach: </strong>We performed Monte Carlo simulations to generate DS FD-NIRS data from two- and three-layer media with a range of layer thicknesses and optical properties. We collected <i>in vivo</i> data with DS FD-NIRS (source-detector distances: 25 and 37 mm, wavelengths: 690 and 830 nm, and modulation frequency: 140 MHz) over the occipital lobe of human subjects during visual stimulation. Simulated and <i>in vivo</i> data were analyzed with diffusion theory for a homogeneous medium, and results were compared for each DS FD-NIRS data type.</p><p><strong>Results: </strong>We found that the main qualitative features of <i>in vivo</i> data could be reproduced by simulated data from a three-layer medium, with a second layer (representing the cerebrospinal fluid in the subarachnoid space) that is less absorbing and less scattering than the other two layers, and with a top layer thickness that represents the combined scalp and skull thickness.</p><p><strong>Conclusions: </strong>A three-layer model is a viable improvement over a homogeneous model to analyze DS FD-NIRS data (or any other fNIRS data) to generate more accurate measurements of cerebral hemodynamic changes without a need for large datasets for tomographic reconstructions.</p>","PeriodicalId":519981,"journal":{"name":"Biophotonics discovery","volume":"3 2","pages":"025003"},"PeriodicalIF":0.0,"publicationDate":"2026-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13107251/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147794750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrastructural analysis of human uncinate fasciculus with coherent anti-Stokes Raman spectroscopy.","authors":"Kelly Perlman, Valérie Pineau Noël, Armand Collin, Justine Major, Murielle Mardenli, Sébastien Jerczynski, Maria Antonietta Davoli, Julien Cohen-Adad, Daniel Côté, Naguib Mechawar","doi":"10.1117/1.BIOS.3.2.025002","DOIUrl":"10.1117/1.BIOS.3.2.025002","url":null,"abstract":"<p><strong>Significance: </strong>Characterizing the ultrastructure of myelin in the human brain is key to understanding the neurobiology of both health and disease. In postmortem human brain tissue, electron microscopy is often technically unfeasible due to poorer tissue quality.</p><p><strong>Aim: </strong>The uncinate fasciculus (UF) is a long-range white matter association tract that connects the anterior temporal lobe with the orbitofrontal cortex. The UF is not present in rodents yet is highly expanded in humans and nonhuman primates. As such, its molecular and ultrastructural properties are virtually unknown.</p><p><strong>Approach: </strong>Here, we develop and validate a spectral-focusing coherent anti-Stokes Raman spectroscopy (sf-CARS) system coupled with a custom AxonDeepSeg segmentation model to characterize UF ultrastructure in the human postmortem brain ( <math><mrow><mi>n</mi> <mo>=</mo> <mn>6</mn></mrow> </math> ).</p><p><strong>Results: </strong>We provide a proof of concept of this new methodological pipeline in the UF temporal segment and observe that the mean axon diameter detected is <math><mrow><mn>0.93</mn> <mtext>  </mtext> <mi>μ</mi> <mi>m</mi> <mo>±</mo> <mn>0.54</mn></mrow> </math> and the mean myelin thickness is <math><mrow><mn>0.48</mn> <mtext>  </mtext> <mi>μ</mi> <mi>m</mi> <mo>±</mo> <mn>0.14</mn></mrow> </math> . We also observe that the UF axons are thicker than those in the anterior cingulate cortex white matter.</p><p><strong>Conclusions: </strong>We detail and validate the full methodology, including tissue fixation and sectioning, sf-CARS acquisition settings, as well as the AxonDeepSeg deep learning model parameters, such that this pipeline can be utilized by others in the field.</p>","PeriodicalId":519981,"journal":{"name":"Biophotonics discovery","volume":"3 2","pages":"025002"},"PeriodicalIF":0.0,"publicationDate":"2026-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13101438/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147794656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Improved endoscope for imaging and cell collection in the fallopian tubes.","authors":"Dominique Gálvez, Andrew Daniel Rocha, Alana G Gonzales, Dilara J Long, Photini Faith Rice, Makayla R Johnson, Ethan Tovar, Bo Lwin, Helen Varghese, Kongit Amaha, David Fishman, John M Heusinkveld, Jennifer Kehlet Barton","doi":"10.1117/1.BIOS.3.2.025001","DOIUrl":"10.1117/1.BIOS.3.2.025001","url":null,"abstract":"<p><strong>Significance: </strong>Early detection of ovarian cancer requires the observation of subtle changes within the fallopian tubes, where serous tubal intraepithelial carcinoma lesions, the putative precursor of high-grade ovarian serous carcinoma, may be present and detectable.</p><p><strong>Aim: </strong>The cell-acquiring fallopian endoscope was designed to detect and interrogate potentially pathological sites in the fallopian tubes via alterations in fluorescence signal and the collection of epithelial cells.</p><p><strong>Approach: </strong>We performed a comprehensive redesign of a first-generation endoscope prototype and demonstrated its performance in whole, human <i>ex vivo</i> fallopian tubes. Through the iterative improvements to the design, the endoscope features improved imaging, cell collection, and general ease of use. Specifically, improvements included a larger core count fiber imaging bundle, a redesigned close-focus lens, more flexible materials, an altered cell collection method, and a lighter-weight handle.</p><p><strong>Results: </strong>With benign fallopian tube samples from three patients, we demonstrate cell collection on the order of <math> <mrow> <msup><mrow><mn>10</mn></mrow> <mrow><mn>4</mn></mrow> </msup> </mrow> </math> cells per collection and imaging capabilities that result in average image intensity ratios.</p><p><strong>Conclusions: </strong>This second-generation endoscope is suitable for the study of intact fallopian tubes.</p>","PeriodicalId":519981,"journal":{"name":"Biophotonics discovery","volume":"3 2","pages":"025001"},"PeriodicalIF":0.0,"publicationDate":"2026-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13101437/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147794745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}