Plant Cell, Tissue and Organ Culture (PCTOC)最新文献_第2页

Genetic transformation of Rhododendron delavayi for anthocyanin synthesis using Agrobacterium-mediated transformation 利用农杆菌介导的杜鹃花花青素合成的遗传转化

Plant Cell, Tissue and Organ Culture (PCTOC) Pub Date : 2024-05-01 DOI: 10.1007/s11240-024-02775-9

Fenfang Long, Weiwei Zuo, Huie Li, Liang Zeng

引用次数: 0

Elimination of cherry virus A from Prunus domestica ‘Besztercei Bt. 2’ using in vitro techniques 利用体外技术消除 Prunus domestica 'Besztercei Bt.2' 中的樱桃病毒 A

Plant Cell, Tissue and Organ Culture (PCTOC) Pub Date : 2024-05-01 DOI: 10.1007/s11240-024-02770-0

Luca Krisztina Szabó, Francesco Desiderio, Z. Kirilla, Attila Hegedűs, Éva Várallyay, Éva Preininger

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Rapid production of abundant transgenic pomegranate (Punica granatum) hairy roots 快速生产大量转基因石榴毛根

Plant Cell, Tissue and Organ Culture (PCTOC) Pub Date : 2024-05-01 DOI: 10.1007/s11240-024-02761-1

Sailendra Singh, Uri Yaritz, Nadav Ramer, D. Holland, Rachel Amir, Li Tian

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Robust and reliable in vitro regeneration of Tossa Jute (Corchorus olitorius L. var. O-9897) 可靠的 Tossa 黄麻（Corchorus olitorius L. var. O-9897）体外再生技术

Plant Cell, Tissue and Organ Culture (PCTOC) Pub Date : 2024-05-01 DOI: 10.1007/s11240-024-02772-y

Md Jakir Hossain, Borhan Ahmed, Afsana Mimi, Rebina Ferdous, Quazi Mosaddeque Hosen

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Callus induction and elicitation for enhanced cyanidin accumulation coupled with antioxidant activities in tropical roses (Rosa spp.) 诱导和激发热带玫瑰（蔷薇属）的胼胝体，以增强其氰苷积累和抗氧化活性

Plant Cell, Tissue and Organ Culture (PCTOC) Pub Date : 2024-05-01 DOI: 10.1007/s11240-024-02762-0

Kalyan Chettri, Jayoti Majumder, S. Gantait

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Targeting E(z) methyltransferase inhibitor GSK126 promotes adventitious root induction in Larix kaempferi 靶向 E（z）甲基转移酶抑制剂 GSK126 可促进山毛榉不定根的诱导

Plant Cell, Tissue and Organ Culture (PCTOC) Pub Date : 2024-05-01 DOI: 10.1007/s11240-024-02769-7

Haifei Hao, Jiaqi Kang, Baohui Xie, Xiangning Jiang, Y. Gai

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Effects of nitrogen deficiency and drought stresses on miRNA expressions in Arabidopsis thaliana 缺氮和干旱胁迫对拟南芥 miRNA 表达的影响

Plant Cell, Tissue and Organ Culture (PCTOC) Pub Date : 2024-05-01 DOI: 10.1007/s11240-024-02754-0

Seda Yaşar, Elif Pulat, Özgür Çakır

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Rose FT homologous gene overexpression affects flowering and vegetative development behavior in two different rose genotypes 玫瑰 FT 同源基因过表达影响两种不同玫瑰基因型的开花和无性发育行为

Plant Cell, Tissue and Organ Culture (PCTOC) Pub Date : 2024-02-26 DOI: 10.1007/s11240-024-02695-8

L. Hamama, J. Bosselut, L. Voisine, T. Thouroude, L. Ogé, J. Chameau, C. Vilfroy, S. Foucrier, S. Pierre, J. Jeauffre, F. Foucher, L. Hibrand-Saint Oyant

{"title":"Rose FT homologous gene overexpression affects flowering and vegetative development behavior in two different rose genotypes","authors":"L. Hamama, J. Bosselut, L. Voisine, T. Thouroude, L. Ogé, J. Chameau, C. Vilfroy, S. Foucrier, S. Pierre, J. Jeauffre, F. Foucher, L. Hibrand-Saint Oyant","doi":"10.1007/s11240-024-02695-8","DOIUrl":"https://doi.org/10.1007/s11240-024-02695-8","url":null,"abstract":"<h3>Abstract</h3> <p>To address the major challenge of controlling flowering in roses, we studied the ectopic expression of a <em>FLOWERING LOCUS T</em> (FT) homolog, which plays an important role in flowering time regulation in plants. We detected seven rose genes of the phosphatidyl ethanolamine-binding protein (PEBP) family and selected one of them (<em>RoFT</em>) based on its co-localization with flowering and architecture QTLs to study its overexpression in two rose genotypes. Embryogenic lines were obtained and genetic transformation was performed on the rose genotypes. Regenerated plantlets were maintained in a greenhouse until flowering and then floral and architectural traits were scored. We observed substantial variation in phenotypic expression between genotypes and between events, especially in the flowering date and number of floral organs. The expression of 14 genes putatively involved in floral initiation (mainly <em>FT</em> targets) and floral development (from the class ABC model) was studied to gain insight into these variations. We noted than target genes are up- or down-regulation and can partially explain the observed phenotype. Our results revealed that a gene ectopically overexpressed in different genetic backgrounds could have diverse effects and that the overexpression <em>RoFT</em> gene can have pleiotropic effects.</p>","PeriodicalId":517397,"journal":{"name":"Plant Cell, Tissue and Organ Culture (PCTOC)","volume":"170 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139981720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancing polyphenol yield in Salvia viridis L. shoot culture through liquid medium optimization and light spectrum manipulation 通过优化液体培养基和光谱操作提高丹参嫩枝培养的多酚产量

Plant Cell, Tissue and Organ Culture (PCTOC) Pub Date : 2024-02-26 DOI: 10.1007/s11240-024-02713-9

Izabela Grzegorczyk-Karolak, Przemysław Tabaka, Izabela Weremczuk-Jeżyna

{"title":"Enhancing polyphenol yield in Salvia viridis L. shoot culture through liquid medium optimization and light spectrum manipulation","authors":"Izabela Grzegorczyk-Karolak, Przemysław Tabaka, Izabela Weremczuk-Jeżyna","doi":"10.1007/s11240-024-02713-9","DOIUrl":"https://doi.org/10.1007/s11240-024-02713-9","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p><i>Salvia viridis</i>, cultivated in Europe as an ornamental plant, has been used for centuries in folk medicine in its natural habitat in the areas of Iran and Turkey. The aim of the study was to obtain a <i>S. viridis</i> shoot culture that can provide high amounts of raw material rich in bioactive compounds at the lowest possible cost in a short time. It investigated the effect of different light spectra (including blue, red, mixed i.e. R:B 7:3 and white LEDs, and fluorescent lamps), and different liquid media systems (liquid agitated medium, liquid stationary medium without support, and with nanocellulose or polyurethane as support) on culture growth and polyphenol production. The phenolic compounds in 80% methanolic extract were quantified by ultra-high performance liquid chromatography (UPLC). It was found that among the LED treatments, the blue light had the most beneficial effect on shoot regeneration (proliferation factor of 4.56), shoot biomass (17-fold increase in fresh weight, 15-fold increase in dry weight), and polyphenol level (15.24 mg/g dry weight). Additionally, a liquid system with nanocellulose as a supporting material turned out to be beneficial for the cultivation of <i>S. viridis</i>. In these conditions, about 8.4 new buds/shoots were obtained from a single explant, fresh weight increased about 40-fold, and dry weight 30-fold. The liquid culture system with cellulose yielded 38.5 mg/g DW polyphenol content: twice as high as the shoots on the solid medium.</p><h3 data-test=\"abstract-sub-heading\">Key message</h3><p>Liquid system with nanocellulose and blue LEDs result in efficient biomass and total polyphenol accumulation in <i>Salvia viridis</i> shoots.</p>","PeriodicalId":517397,"journal":{"name":"Plant Cell, Tissue and Organ Culture (PCTOC)","volume":"139 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139981719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0