Sustainable microbiology最新文献_第2页

Microbial water quality investigation through flow cytometry fingerprinting: from source to tap 通过流式细胞仪指纹图谱进行微生物水质调查：从水源到水龙头

Sustainable microbiology Pub Date : 2024-02-14 DOI: 10.1093/sumbio/qvae003

L. Claveau, N. Hudson, P. Jarvis, P. Jeffrey, F. Hassard

{"title":"Microbial water quality investigation through flow cytometry fingerprinting: from source to tap","authors":"L. Claveau, N. Hudson, P. Jarvis, P. Jeffrey, F. Hassard","doi":"10.1093/sumbio/qvae003","DOIUrl":"https://doi.org/10.1093/sumbio/qvae003","url":null,"abstract":"\u0000 Ensuring the quality of treated drinking water is crucial for preventing potential health impacts, regulatory fines, and reputation damage. Traditional culture-based microbiological methods often fail to capture the heterogeneity of the bacterial communities in drinking water. This study employed daily interstage monitoring and flow cytometry analysis over a period of one year to investigate the dynamics of water treatment processes and service reservoirs. The objective of this study was to test the utility of flow cytometry fingerprints for aiding in microbial event detection. We found that the chlorine concentration contact time (Ct) was pivotal for microbial log reduction across the treatment works. Flow cytometry fingerprints exhibited significant deviations during operational events, such as process interruptions, but did not correlate with the presence of bacterial indicator organisms in the distributed and tap water. Furthermore, the diversity of bacterial fingerprints, quantified by the Bray-Curtis dissimilarity index, served as an indicator for identifying potentially poor microbial water quality. In chlorinated waters with low cell counts, the background signal shows potential as a metric to differentiate between different water sources, thereby offering the possibility to characterise breakthrough events in these circumstances that challenge most other microbial analytical methods. Interestingly, groundwater from simpler treatment works showed a higher occurrence of bacterial indicators, whereas surface water works had a lower incidence. These findings underpin the importance of appropriate disinfection even for “low-risk” source waters and the added value that the statistical interpretation of flow cytometry data can offer objective decision making.","PeriodicalId":516860,"journal":{"name":"Sustainable microbiology","volume":"108 9","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139963524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Examining the taxonomic distribution of tetracycline resistance in a wastewater plant. 研究污水处理厂中四环素耐药性的分类分布。

Sustainable microbiology Pub Date : 2024-01-19 eCollection Date: 2024-01-01 DOI: 10.1093/sumbio/qvad003

Howard Ochman, Erik M Quandt, Neil Gottell, Jack A Gilbert

{"title":"Examining the taxonomic distribution of tetracycline resistance in a wastewater plant.","authors":"Howard Ochman, Erik M Quandt, Neil Gottell, Jack A Gilbert","doi":"10.1093/sumbio/qvad003","DOIUrl":"10.1093/sumbio/qvad003","url":null,"abstract":"Microbial communities serve as reservoirs of antibiotic resistance genes (ARGs) and facilitate the dissemination of these genes to bacteria that infect humans. Relatively little is known about the taxonomic distribution of bacteria harboring ARGs in these reservoirs and the avenues of transmission due to the technical hurdles associated with characterizing the contents of complex microbial populations and the assignment of genes to particular genomes. Focusing on the array of tetracycline resistance (Tcr) genes in the primary and secondary phases of wastewater treatment, 17 of the 22 assayed Tcr genes were detected in at least one sample. We then applied emulsion, paired isolation, and concatenation PCR (epicPCR) to link tetracycline resistance genes to specific bacterial hosts. Whereas Tcr genes tend to vary in their distributions among bacterial taxa according to their modes of action, there were numerous instances in which a particular Tcr gene was associated with a host that was distantly related to all other bacteria bearing the same gene, including several hosts not previously identified. Tcr genes are far less host-restricted than previously assumed, indicating that complex microbial communities serve as settings where ARGs are spread among divergent bacterial phyla.","PeriodicalId":516860,"journal":{"name":"Sustainable microbiology","volume":"1 1","pages":"qvad003"},"PeriodicalIF":0.0,"publicationDate":"2024-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10840452/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139693787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbial threats and sustainable solutions for molluscan aquaculture 软体动物水产养殖的微生物威胁和可持续解决方案

Sustainable microbiology Pub Date : 2024-01-19 DOI: 10.1093/sumbio/qvae002

Emily Kunselman, Kara Wiggin, Rachel E. Diner, Jack A. Gilbert, Sarah M Allard

{"title":"Microbial threats and sustainable solutions for molluscan aquaculture","authors":"Emily Kunselman, Kara Wiggin, Rachel E. Diner, Jack A. Gilbert, Sarah M Allard","doi":"10.1093/sumbio/qvae002","DOIUrl":"https://doi.org/10.1093/sumbio/qvae002","url":null,"abstract":"\u0000 Aquaculture is responsible for producing almost half of the world's seafood. As the global climate changes and population continues to increase, we must prepare for increased disease in aquatic animals, a risk compounded by high density aquafarms that are necessary to keep up with demand. This review will highlight major microbial threats to aquaculture and current and alternative solutions to these threats with consideration for the accessibility of the proposed solutions. Molluscs are ideal for sustainable aquaculture because they require less inputs than most other protein sources, and through filter feeding they improve local ecosystem health. However, they are also plagued by microbial diseases, and rising water temperatures will only exacerbate this problem by enhancing pathogen survival, range, and growth. At the same time, microbial treatments hold great promise for reducing disease burden and increasing yield and food safety. In order to combat threats to sustainable aquaculture, it is critical to monitor and predict microbial behavior in coastal water and animal populations, explore sustainable microbial treatment options such as probiotics and phage therapy, reduce reliance on antimicrobials, and develop mitigation strategies through partnership with mollusc farmers, government regulators, industry, academic researchers, and indigenous peoples.","PeriodicalId":516860,"journal":{"name":"Sustainable microbiology","volume":"11 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140503506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oxalate and oxalotrophy: an environmental perspective 草酸盐和草营养不良：环境视角

Sustainable microbiology Pub Date : 2024-01-17 DOI: 10.1093/sumbio/qvad004

D. A. Cowan, D. Babenko, R. Bird, A. Botha, D. O. Breecker, C. E. Clarke, M. L. Francis, T. Gallagher, P. Lebre, T. Nel, A. J. Potts, M. Trindade, L. Van Zyl

{"title":"Oxalate and oxalotrophy: an environmental perspective","authors":"D. A. Cowan, D. Babenko, R. Bird, A. Botha, D. O. Breecker, C. E. Clarke, M. L. Francis, T. Gallagher, P. Lebre, T. Nel, A. J. Potts, M. Trindade, L. Van Zyl","doi":"10.1093/sumbio/qvad004","DOIUrl":"https://doi.org/10.1093/sumbio/qvad004","url":null,"abstract":"\u0000 Oxalic acid is one of the most abundant organic acids produced by plants. Much of the global production of oxalic acid is deposited on soil surfaces in leaf litter, to be oxidised by microorganisms, resulting in a pH increase and shifting the carbonate equilibria. In what is known as the oxalate-carbonate pathway, calcium oxalate metabolism results in CO2 being sequestered into soils as insoluble calcite (CaCO3). There is a growing appreciation that the global scale of this process is sufficiently large to be an important contribution to global carbon turnover budgets.\u0000 The microbiomics, genetics and enzymology of oxalotrophy are all soundly established, although a more detailed understanding of the landscape-scale kinetics of the process would be needed to incorporate oxalotrophy as an element of process models informing the relevant Sustainable Development Goals. Here, we review the current state of knowledge of oxalotrophs and oxalotrophy and the role they play in terrestrial ecosystem services and functions in terms of carbon sequestration and nutrient cycling. We emphasize the relevance of these to the Sustainability Development Goals (SDGs) and highlight the importance of recognising oxalotrophy when accounting for the natural capital value of an ecosystem.","PeriodicalId":516860,"journal":{"name":"Sustainable microbiology","volume":"70 2-3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140505262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antimicrobial-resistant pathogens in fruits and vegetables from retail and home gardens 零售和家庭菜园蔬果中的抗菌病原体

Sustainable microbiology Pub Date : 2024-01-01 DOI: 10.1093/sumbio/qvad002

A. Olanbiwoninu, Theresa Awotundun, J. Olayiwola, Y. Somorin

{"title":"Antimicrobial-resistant pathogens in fruits and vegetables from retail and home gardens","authors":"A. Olanbiwoninu, Theresa Awotundun, J. Olayiwola, Y. Somorin","doi":"10.1093/sumbio/qvad002","DOIUrl":"https://doi.org/10.1093/sumbio/qvad002","url":null,"abstract":"\u0000 Fruits and vegetables have been identified as vehicles for the transmission of pathogenic antimicrobial-resistant (AMR) microorganisms. This is of food safety concern, thus requiring continuous surveillance. This study aimed to profile AMR bacteria present in selected fruits and vegetables retailed in markets and from home gardens (HGs) in Oyo, Ogun, and Ekiti states, Nigeria. Watermelon, cucumber, tomato, and garden egg samples were collected and analyzed using standard microbiological procedures. The susceptibility of the isolates to eight antibiotics was determined. Multidrug-resistant isolates were screened for the presence of AMR genes by polymerase chain reaction. Fifty three bacteria were isolated and identified, belonging to the genera Bacillus, Corynebacterium, Listeria, Aeromonas, Enterobacter, Erwinia, Salmonella, Serratia, Shigella, and Vibrio. Thirty six (67.93%) isolates demonstrated phenotypic resistance to five of the eight antibiotics tested, being the most prevalent pattern observed. BlaTEM and blaCTX-M were detected in Salmonella enterica from retailed tomato, blaTEM, blaSHV, blaCTX-M, and erm(B) were detected in Listeria monocytogenes from retailed watermelon, blaSHV and blaCTX-M were detected in Bacillus cereus from retailed tomato, while blaTEM, blaSHV, blaCTX-M, and erm(F) were detected in Staphylococcus aureus isolated in garden egg from HG. The presence of multidrug-resistant pathogens in fruits and vegetables could pose a huge food safety and public health risk.","PeriodicalId":516860,"journal":{"name":"Sustainable microbiology","volume":"17 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140520477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to: Oxalate and oxalotrophy: an environmental perspective 更正：草酸盐和草营养不良：环境视角

Sustainable microbiology Pub Date : 2024-01-01 DOI: 10.1093/sumbio/qvae004

引用次数: 0

Announcing sustainable microbiology: How microbes make a sustainable world 发布可持续微生物学：微生物如何创造一个可持续的世界

Sustainable microbiology Pub Date : 2024-01-01 DOI: 10.1093/sumbio/qvae001

David A Pearce

引用次数: 0

Evaluating the National Action Plan (NAP) on antimicrobial resistance, and recommendations for the next 5-year NAP: a roundtable discussion 评估《抗菌药耐药性国家行动计划》（NAP），并就下一个五年期《国家行动计划》提出建议：圆桌讨论会

Sustainable microbiology Pub Date : 2024-01-01 DOI: 10.1093/sumbio/qvad001

Daisy Neale, Lucky Cullen

{"title":"Evaluating the National Action Plan (NAP) on antimicrobial resistance, and recommendations for the next 5-year NAP: a roundtable discussion","authors":"Daisy Neale, Lucky Cullen","doi":"10.1093/sumbio/qvad001","DOIUrl":"https://doi.org/10.1093/sumbio/qvad001","url":null,"abstract":"\u0000 The UK’s current 5-year National Action Plan (NAP) on tackling antimicrobial resistance (AMR), which runs from 2019 to 2024, is coming to an end. As such, the next 5-year NAP is currently under development, and progress made towards the UK’s commitments on AMR is being analysed. This analysis has involved the publication of an addendum to the current NAP, which includes some new and revised commitments. Applied Microbiology International (AMI) held a roundtable discussion in November 2022, inviting UK experts from across the human and animal sectors to discuss their thoughts on progress to date, as well as any recommendations and key considerations they thought should be taken forward. Overall, it was agreed that there is a major disparity between the increasing ambitions of the NAP, and the reducing financial support for achieving said ambitions. The gap between evidence generation and implementing recommendations into real-world settings also presents a key challenge to overcome, if the NAPs commitments are to be achieved. A lack of communication and funding appear to underpin many of the barriers that prevent the successful delivery of current NAP commitments and are therefore highlighted as key areas of concern for policymakers to consider when looking to tackle AMR. The focus should be on successfully implementing and delivering the current NAP commitments ahead of identifying future ambitions.","PeriodicalId":516860,"journal":{"name":"Sustainable microbiology","volume":"35 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140519517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0