Avian diseases最新文献

{"title":"Note from Editor.","authors":"Randall Singer","doi":"10.1637/aviandiseases-D-25-250022","DOIUrl":"https://doi.org/10.1637/aviandiseases-D-25-250022","url":null,"abstract":"","PeriodicalId":516846,"journal":{"name":"Avian diseases","volume":"68 S1","pages":"361"},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144057042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Neurological Presentation Associated with Meningoencephalitis Caused by <i>Streptococcus gallolyticus</i> Subsp. <i>pasteurianus</i> in Turkey Poults.","authors":"LaTasha S Gray, Kabel Robbins, Elise Gerken, Timothy J Johnson, Randle W Moore, Alicia Amsden, Troy J Warfield, Billy M Hargis, Maria K Dashek","doi":"10.1637/aviandiseases-D-24-00021","DOIUrl":"https://doi.org/10.1637/aviandiseases-D-24-00021","url":null,"abstract":"<p><p>The current case report describes two outbreaks of central nervous system signs associated with <i>Streptococcus gallolyticus</i> subsp. <i>pasteurianus</i> causing encephalitis and meningitis in poults in the U.S. Midwest. Macroscopic lesions observed at necropsy revealed enlarged and pale spleens as well as blood vessel congestion of the meninges. Histopathological examination revealed moderately severe meningoencephalitis with coccoid bacteria detected within the macrophages and mild heterophilic portal hepatitis. Bacterial identification confirmed <i>S. gallolyticus</i> subsp. <i>pasteurianus</i> by matrix-assisted laser desorption time-of-flight mass spectrometry and API 20 STREP strips. Antibiotic sensitivity profiles of the two outbreaks were similar and revealed sensitivity to clindamycin, erythromycin, florfenicol, gentamycin, penicillin, and ceftiofur. Genomic comparisons revealed that the two isolates presented here were identical and encoded for a putative collagen adhesin that could be a potential virulence factor.</p>","PeriodicalId":516846,"journal":{"name":"Avian diseases","volume":"68 S1","pages":"455-460"},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144003529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and Validation of PCR Assays for Improved Diagnosis of Infectious Coryza by Differentiating Pathogenic and Nonpathogenic <i>Avibacterium paragallinarum</i>.","authors":"Mostafa M S Shelkamy, Amro Hashish, Maria Chaves, Mariela E Srednik, Nubia R Macedo, Eman Gadu, Yuko Sato, Stephan Schmitz-Esser, Qijing Zhang, Mohamed El-Gazzar","doi":"10.1637/aviandiseases-D-24-00041","DOIUrl":"https://doi.org/10.1637/aviandiseases-D-24-00041","url":null,"abstract":"&lt;p&gt;&lt;p&gt;&lt;i&gt;Avibacterium paragallinarum&lt;/i&gt; (AP) is a primary bacterial pathogen of chickens that leads to infectious coryza (IC) disease. Recently, multiple commercial layer flocks in several U.S. states reported positive real-time quantitative PCR (qPCR) results without any history of clinical signs. Owing to the proven specificity of the current IC qPCR assays, these results suggested the existence of AP strains that do not lead to clinical disease in layers, i.e., nonpathogenic AP (npAP) strains. This was further proven by isolating and characterizing npAP strains from these normal layer flocks. Although these strains are clinically nonpathogenic in layers, current IC qPCR assays fail to distinguish them from the pathogenic AP, leading to qPCR-positive flocks with no apparent disease. Therefore, the purpose of this study was to develop and validate TaqMan real-time PCR assays that can differentiate between pathogenic and the newly discovered npAP strains. Whole-genome sequences of six npAP isolates were generated, and genomic comparison was conducted against 43 pathogenic AP strains. Analysis revealed two consistent features. First, the capsular polysaccharide transporter gene &lt;i&gt;hctA&lt;/i&gt; was exclusively present in the pathogenic AP strains but absent in npAP strains. Second, unique lengthy insertions within the &lt;i&gt;HMTp210&lt;/i&gt; gene were observed only in the npAP strains. The &lt;i&gt;HMTp210&lt;/i&gt; insertions were chosen as the qPCR target to identify the newly discovered npAP strains (np-&lt;i&gt;HMTp210&lt;/i&gt; assay). On the other hand, &lt;i&gt;hctA&lt;/i&gt; was selected to identify the pathogenic AP strains. During the validation process, 28 isolates and 10 oropharyngeal (OP) swab pools representing the pathogenic AP strains as well as six isolates and 86 OP pools of npAP strains were tested. A wide panel of respiratory, bacterial and viral, pathogens were included in the validation. Both assays demonstrated high performance in terms of analytical specificity in relation to each other and when tested against various bacterial and viral pathogens. Moreover, the &lt;i&gt;hctA&lt;/i&gt; and &lt;i&gt;np-HMTp210&lt;/i&gt; assays displayed high sensitivity, with a limit of detection of 1 copy/µl and 2.5 copies/µl, respectively, and PCR efficiencies of 94.62% and 92.99%, respectively. Both assays showed 100% diagnostic specificity and sensitivity. However, after the validation process, an ongoing surveillance effort in clinically normal layer flocks uncovered a new population of npAP strains. This new npAP population refutes our original qPCR design goals to distinguish AP strains from npAP strains because the latest finding renders the differential capacity of this newly developed PCR incomplete. However, the newly developed qPCR in its current status is still useful in differentiating the great majority of cases and is still useful for diagnostic laboratories to provide much needed IC diagnostic answers to the poultry industry. Meanwhile, we will continue to investigate new targets that could eithe","PeriodicalId":516846,"journal":{"name":"Avian diseases","volume":"68 S1","pages":"380-390"},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144060012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessing the Efficacy of Oregano, Apple Cider Vinegar, and Citric Acid in Water as a Treatment for Spotty Liver Disease Caused by <i>Campylobacter hepaticus</i> in Challenged SPF Laying Hens.","authors":"Roel Becerra, Monique S Franca, Catherine M Logue","doi":"10.1637/aviandiseases-D-24-00063","DOIUrl":"https://doi.org/10.1637/aviandiseases-D-24-00063","url":null,"abstract":"&lt;p&gt;&lt;p&gt;Spotty liver disease (SLD) caused by &lt;i&gt;Campylobacter hepaticus&lt;/i&gt; has emerged as an important cause of disease in table egg layers in the United States. The disease associated with &lt;i&gt;C. hepaticus&lt;/i&gt; results in focal lesions on the livers of infected birds, reduced egg production, and increased mortality of layer hens. Currently, there are no approved treatments and no commercial vaccine available for &lt;i&gt;C. hepaticus&lt;/i&gt;, and very limited research is available supporting best approaches for the control of &lt;i&gt;C. hepaticus&lt;/i&gt; in organic or antibiotic free-laying hens. We assessed the potential effects of water acidification treatments for the control of &lt;i&gt;C. hepaticus&lt;/i&gt; in challenged specific-pathogen-free (SPF) chickens and transmission of the organism to naïve birds. The objective of this study was to develop a challenge and treatment model to assess the effects of oregano, apple cider vinegar, and citric acid as potential control measures for &lt;i&gt;C. hepaticus&lt;/i&gt; in challenged birds. We divided 148 SPF chickens, 17 wk of age, into five groups; four groups (with 51% of the birds in each group orally challenged with &lt;i&gt;C. hepaticus&lt;/i&gt;) and one negative control group (&lt;i&gt;n&lt;/i&gt; = 8). A dose of 10&lt;sup&gt;7&lt;/sup&gt; cfu/ml of &lt;i&gt;C. hepaticus&lt;/i&gt; was given on day 1, day 4, and day 7 by oral gavage. At day 10 after the day 1 challenge, three challenged groups were treated for 5 days with either oregano, apple cider vinegar, or citric acid in the water. One challenged but not treated group and the negative control received only water as treatment. Bile and liver samples from challenged and nonchallenged naïve exposed birds were collected from euthanatized birds at days 9, 15, 20, and 27 after the first day challenge to evaluate the presence of &lt;i&gt;C. hepaticus&lt;/i&gt; gross lesions and detection via culture and polymerase chain reaction (PCR) confirmation. Results showed that challenged birds were able to develop mild to moderate multifocal liver lesions resembling SLD. Severe lesions were evident in the naïve, exposed nonchallenged birds. Positive bacterial culture and colony PCR positives were found in challenged birds in each challenge group. Results from PCR of DNA extracted from liver samples were able to detect a greater number of &lt;i&gt;C. hepaticus&lt;/i&gt;-positive samples than culture PCR of bile alone. Treatment with oregano, apple cider vinegar, or citric acid was not able to clear &lt;i&gt;C. hepaticus&lt;/i&gt; from challenged birds because more than 44% of the challenged birds remained positive on liver PCR analysis posttreatment. Results also found that &lt;i&gt;C. hepaticus&lt;/i&gt; could be passed horizontally because more than 29% of the naïve nonchallenged birds became &lt;i&gt;C. hepaticus&lt;/i&gt; positive postintroduction to challenged birds. Overall, this study found that this challenge model for &lt;i&gt;C. hepaticus&lt;/i&gt; was successful and that treatments did not clear &lt;i&gt;C. hepaticus&lt;/i&gt; in challenged birds. Nevertheless, it appears that acidifying the water could help to re","PeriodicalId":516846,"journal":{"name":"Avian diseases","volume":"68 S1","pages":"490-498"},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144056616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Emergence of <i>Streptococcus gallolyticus</i> as a Pathogen in Turkeys.","authors":"LaTasha S Gray, Timothy J Johnson, Kabel M Robbins, Billy M Hargis, Juan D Latorre","doi":"10.1637/aviandiseases-D-24-00097","DOIUrl":"https://doi.org/10.1637/aviandiseases-D-24-00097","url":null,"abstract":"<p><p><i>Streptococcus gallolyticus</i>, formerly known as <i>Streptococcus bovis</i>, is a Gram-positive coccus bacterium that is a facultative anaerobe. Although it was previously considered a commensal bacterium that caused secondary disease because of a stressor, <i>S. gallolyticus</i> can now be considered a causative agent of disease associated with a variety of infections in both humans and animals. <i>Streptococcus gallolyticus</i> has become an emerging pathogen in the poultry industry, particularly in turkey poults, causing acute mortality, usually without any premonitory signs. In the southern portion of the United States, there has been an influx of <i>S. gallolyticus</i> infections occurring predominately in July and August. A majority of turkey poults most susceptible to this disease range in age between 1.5 and 2.5 wk of age. It is suggested that virulence capability relies on accessory genome components that may not be core to all <i>S. gallolyticus</i> strains. In this review, we aim to provide an overview of the nomenclature changes, identification, and emergence of <i>S. gallolyticus</i> in turkeys.</p>","PeriodicalId":516846,"journal":{"name":"Avian diseases","volume":"68 S1","pages":"448-454"},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144057053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An Updated Review of <i>Enterococcus cecorum</i> Infections in Poultry.","authors":"J Higuita, M Arango, A Forga, D Cortes, D Graham","doi":"10.1637/aviandiseases-D-24-00098","DOIUrl":"https://doi.org/10.1637/aviandiseases-D-24-00098","url":null,"abstract":"<p><p>Since the early 2000s, skeletal effects, specifically enterococcal spondylitis, related to pathogenic <i>Enterococcus cecorum</i> (EC), have been observed in older broiler chicken flocks. This skeletal involvement has typically been associated with persistent EC infections in the free thoracic vertebrae leading to paralysis. However, the emergence of virulent EC in young broiler chicken flocks causing clinical septicemia requires further investigation. The purpose of this review is to provide an update on EC-related research and pending industry needs.</p>","PeriodicalId":516846,"journal":{"name":"Avian diseases","volume":"68 S1","pages":"404-411"},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144063929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Enterococcus hirae</i>-Associated Endocarditis Outbreak in Young Broiler Breeders of the Female Line.","authors":"Thijs T M Manders, Henk K Parmentier, Andreas Papanikolaou, Rodrigo A Gallardo","doi":"10.1637/aviandiseases-D-24-00025","DOIUrl":"https://doi.org/10.1637/aviandiseases-D-24-00025","url":null,"abstract":"<p><p>Enterococci infections may result in acute and chronic disease. Lesions, such as osteomyelitis, arthritis, or endocarditis, depend on the <i>Enterococcus</i> species involved. In this case report, an outbreak of <i>Enterococcus hirae</i>-associated endocarditis affecting young broiler breeders is presented. On a rearing farm with 46,500 broiler breeders, a slight increased mortality (1.02%-1.42%) within the female line was observed between 2 and 6 wk posthatch (ph). Between days 12 and 47 ph, 256 birds were pathologically examined, and bacteriologic samples were taken. In 191 (51.8%) of the examined female line chickens, endocarditis was observed on at least one of the atrioventricular valves, while no endocarditis was diagnosed in chickens of the male line (<i>n</i> = 65). <i>Enterococcus hirae</i> was isolated from 64.4% of the bone marrow samples and from two heart samples taken from chickens of the female line. <i>Enterococcus hirae</i> was not isolated from samples taken from the male line. In addition, natural antibody titers (immunoglobulin G) were significantly higher in serum of the female line compared with the male line. This case gives an overview of gross lesions and bacteriology of <i>E. hirae</i> affecting only the female line of broiler breeders.</p>","PeriodicalId":516846,"journal":{"name":"Avian diseases","volume":"68 S1","pages":"443-447"},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144045385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and Application of Optimized Isolation Methods and Diagnostic PCR Protocols for the Detection of Pathogenic <i>Enterococcus cecorum</i> Isolated from Broiler Chickens.","authors":"M Mitsu Suyemoto, Grayson K Walker, Undine Taldo, Kayleigh R Diveley, Luke B Borst","doi":"10.1637/aviandiseases-D-24-00067","DOIUrl":"https://doi.org/10.1637/aviandiseases-D-24-00067","url":null,"abstract":"<p><p><i>Enterococcus cecorum</i> (EC) is a dominant enteric commensal in broiler chickens. However, pathogenic strains of EC cause increased morbidity and mortality from septicemic disease in broiler production worldwide. EC infections can present as pericarditis and paralytic spinal lesions from which pathogenic EC can be isolated. However, the inability to distinguish between commensal and pathogenic EC strains has confounded the search for the source of pathogenic EC in environmental or hatchery samples. This issue is exacerbated by poor sensitivity of standard sampling and culture methods. Comparative genomic analysis of EC isolates previously identified a conserved capsule region in pathogenic EC strains that is absent or variable in commensal strains. Based on a capsular synthesis gene, <i>cps</i>O, and EC species-specific <i>sod</i>A primers, we designed a standard multiplex PCR to distinguish pathogenic EC from commensal EC strains. To allow for increased sample throughput, a real-time PCR protocol was developed in tandem based on detection of these genes. To increase the culture sensitivity, a selective enrichment protocol using Todd-Hewitt Broth with 1% yeast extract and four antibiotics enabled the isolation of pathogenic EC from egg transfer residue and culled eggs at hatcheries. Pulsed-field gel electrophoresis was used to genotype recovered hatchery isolates, which identified clonal pathogenic EC strains isolated from hatchery residue and a spinal lesion of a broiler. The ability to distinguish pathogenic EC from commensal EC coupled with modified culture methods will facilitate improved surveillance of pathogenic EC throughout broiler production, ideally leading to decreased incidence or eradication of this disease.</p>","PeriodicalId":516846,"journal":{"name":"Avian diseases","volume":"68 S1","pages":"427-434"},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144048912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>In vitro</i> Evaluation of Candidate <i>Bacillus</i> Strains Against <i>erysipelothrix rhusiopathiae</i> from Erysipelas Outbreaks in Layer Flocks.","authors":"Diana I Ayala, Nick P Evans, Dan Wilson, Mark Mouw, T Peter Karnezos","doi":"10.1637/aviandiseases-D-24-00061","DOIUrl":"https://doi.org/10.1637/aviandiseases-D-24-00061","url":null,"abstract":"<p><p>Erysipelas is a zoonotic disease, causing acute infections in swine, poultry, and a wide variety of animals, including humans. In layers, erysipelas is considered an emerging disease characterized by hepatomegaly, splenomegaly, septicemia, and acute death. It affects flocks between 43 and 73 wk old and can result in 50% overall mortality. Layer hens possibly affected by an erysipelas outbreak were sampled in this study. Organs at necropsy showed the typical erysipelas lesions; the presence of <i>Erysipelothrix rhusiopathiae</i> was confirmed by selective plating and matrix-assisted laser desorption ionization time-of-flight mass spectrometry. The antimicrobial effect of a set of 20 proprietary <i>Bacillus</i> direct-fed microbials (DFM), individual strains and in combinations, was analyzed against confirmed <i>E. rhusiopathiae</i> isolates, by agar-well diffusion assay. Nine of the <i>Bacillus</i> strains screened in this study were found effective at inhibiting the growth of all four <i>E. rhusiopathiae</i> isolates from erysipelas cases. The findings of this study highlight the potential of using the <i>Bacillus</i> DFMs <i>in vivo</i> to evaluate their efficacy as biocontrol alternatives to reduce the growth <i>E. rhusiopathiae</i> in poultry production.</p>","PeriodicalId":516846,"journal":{"name":"Avian diseases","volume":"68 S1","pages":"521-524"},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144003526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Evolving Landscape of <i>Ornithobacterium rhinotracheale</i> in Turkeys: A Review.","authors":"Jennifer Irwin, Timothy J Johnson, Jessica Walters","doi":"10.1637/aviandiseases-D-24-00073","DOIUrl":"https://doi.org/10.1637/aviandiseases-D-24-00073","url":null,"abstract":"<p><p><i>Ornithobacterium rhinotracheale</i> (ORT) is a Gram-negative, pleomorphic rod-shaped bacterium that causes respiratory disease in the commercial poultry industry. This bacterium has been causing severe disease in chickens and turkeys since it emerged in the 1980s. In birds, increased mortality, reduced egg production, and loss in weight gain gives ORT the potential to have severe economic implications. In older birds, ORT causes more severe lesions and increases in mortality; therefore it is of particular concern in the breeder industry because infection and loss of production in breeders impact all stages of production. Despite the importance of ORT, few published studies have contributed to understanding of the diagnostics, treatment, and prevention of this disease. This review discusses the evolving landscape of ORT and summarizes an update on important issues related to ORT.</p>","PeriodicalId":516846,"journal":{"name":"Avian diseases","volume":"68 S1","pages":"461-468"},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143996624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}