The Egyptian journal of immunology最新文献_第5页

Interleukin-8 genetic polymorphism and its relation to Helicobacter pylori infection and Helicobacter pylori-associated gastric diseases. 白细胞介素-8基因多态性及其与幽门螺旋杆菌感染和幽门螺旋杆菌相关胃病的关系

The Egyptian journal of immunology Pub Date : 2024-01-01 DOI: 10.55133/eji.310106

Ibrahim A Amin, Mona A Hassan, S. Elgendy, A. Abdelmohsen, Mamdouh Y Ali, Bahaa-Eldin A Abdel-Raady

{"title":"Interleukin-8 genetic polymorphism and its relation to Helicobacter pylori infection and Helicobacter pylori-associated gastric diseases.","authors":"Ibrahim A Amin, Mona A Hassan, S. Elgendy, A. Abdelmohsen, Mamdouh Y Ali, Bahaa-Eldin A Abdel-Raady","doi":"10.55133/eji.310106","DOIUrl":"https://doi.org/10.55133/eji.310106","url":null,"abstract":"Helicobacter pylori (H. pylori) infection has a variety of clinical outcomes, and host genetic factors play an important role in this process. Cytokines are important factors in mediating and controlling the inflammatory process during H. pylori infection. Interleukin-8 (IL-8) plays a critical role in the epithelial cell response to H. pylori infection and the development of H. pylori-related gastric disorders. The IL-8 gene has an A/T base pair polymorphism in the promoter region (-251), which has been linked to an increase in interleukin production by gastric epithelial cells. In this context, the goal of our study was to determine the polymorphism in the IL-8 gene and its relation to H. pylori infection and H. pylori-associated gastric diseases. Gastric biopsy specimens were collected from 44 patients with H. pylori infection and 29 patients without H. pylori infection. The rapid urease test and detection of the glmM gene were used to diagnose H. pylori infection. Polymerase chain reaction-restriction fragment length polymorphism was used to identify the polymorphism in the Il-8 gene (at position-251). The presence of the A/A and T/A genotypes of the IL-8 gene was found to be significantly associated with susceptibility to H. pylori infection (p = 0.012 and p = 0.004, respectively). Also, the IL-8 A allele was significantly associated with H. pylori infection in our study (p = 0.002). We did not find a significant association between IL-8 gene polymorphism and a higher risk of gastritis and peptic ulcer disease. In conclusion, IL-8 gene polymorphism at -251 position was significantly associated with H. pylori infection.","PeriodicalId":516584,"journal":{"name":"The Egyptian journal of immunology","volume":"1 4","pages":"48-57"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140525975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessment of long noncoding RNA CCAT1 using real time-polymerase chain reaction in colorectal cancer patients. 利用实时聚合酶链反应评估结直肠癌患者体内的长非编码 RNA CCAT1

The Egyptian journal of immunology Pub Date : 2024-01-01 DOI: 10.55133/eji.310111

Amal A Mahmoud, Hanan O Mohamed, Mahmoud R Shehata, Alyaa A S Refae, Mostafa H Abd El Salam, M. I. Seddik

{"title":"Assessment of long noncoding RNA CCAT1 using real time-polymerase chain reaction in colorectal cancer patients.","authors":"Amal A Mahmoud, Hanan O Mohamed, Mahmoud R Shehata, Alyaa A S Refae, Mostafa H Abd El Salam, M. I. Seddik","doi":"10.55133/eji.310111","DOIUrl":"https://doi.org/10.55133/eji.310111","url":null,"abstract":"Colorectal cancer (CRC) is linked to high mortality, mainly when discovered in its advanced stages. Several studies have pointed to the role of epigenetic factors in CRC and other cancers. Long non-coding RNAs (lncRNAs) are involved in the initiation, progression, metastasis, and modulation of the response to chemotherapeutic modalities of CRC as vital contributors to epigenetic mechanisms. Colon cancer-associated transcript-1 (CCAT1) is one of the lncRNAs that have been dysregulated in serum samples, providing a non-invasive route for diagnosing CRC patients. This study aimed to determine the role of CCAT1 expression as diagnostic and prognostic markers. We tested the associations of CCAT1 expression with serum carcinoembryonic antigen (CEA) and carbohydrate antigen 19-9 (CA 19-9). The study included three groups: 41 patients with colorectal cancer, 39 patients with precancerous benign colorectal diseases, and 20 normal control individuals. CEA and CA 19-9 were measured by an immunoassay automated system. The expression level of CCAT1 was assessed by a real-time polymerase chain reaction. There was a statistically significant elevation of serum CEA levels in patients with CRC compared to patients with precancerous benign colorectal diseases. Furthermore, there was no statistically significant difference in serum CA 19-9 levels between all groups (p = 0.102). Interestingly, CCAT1 expression was significantly upregulated in the blood of CRC patients compared to the precancerous benign colorectal diseases group (p = 0.009) and the control group (p <0.001). Also, expression of CCAT1 was significantly elevated in patients with precancerous benign colorectal diseases compared to the control group (p=0.004). In conclusion, measuring the expression level of CCAT1 is more advised than assessment of CEA and CA 19-9 for the early diagnosis and prognosis of colorectal cancer.","PeriodicalId":516584,"journal":{"name":"The Egyptian journal of immunology","volume":"31 9","pages":"106-115"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139640718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prevalence of viral hepatitis (A, B, C, and E) infection and co-infection among hospitalized children in Cairo, Egypt. 埃及开罗住院儿童病毒性肝炎（甲型、乙型、丙型和戊型）感染和合并感染的流行率。

The Egyptian journal of immunology Pub Date : 2024-01-01 DOI: 10.55133/eji.310104

Enas Mostafa, Reem El-Shenawy, Ashraf Tabll, S. Shoman

{"title":"Prevalence of viral hepatitis (A, B, C, and E) infection and co-infection among hospitalized children in Cairo, Egypt.","authors":"Enas Mostafa, Reem El-Shenawy, Ashraf Tabll, S. Shoman","doi":"10.55133/eji.310104","DOIUrl":"https://doi.org/10.55133/eji.310104","url":null,"abstract":"Viral hepatitis is considered a public health issue facing the entire world. The World Health Organization encouraged all countries to work together to eliminate this fatal infection and achieve the 2030 agenda. The present study aimed to investigate the silent infection of viral hepatitis (A, B, C, and E) among hospitalized children in Cairo, Egypt, to control and avoid chronic infection early on. This cross-sectional study included 184 randomly selected hospitalized children from three different hospitals in Cairo, Egypt. They were children aged between a few months to 15 years to determine viral hepatitis infection and co-infection. Antibodies to hepatitis A virus (HAV IgM), hepatitis E virus (HEV IgM), hepatitis C virus (HCV Ab), and hepatitis B virus surface antigen (HBs Ag) were performed by ELISA. If the ELISA results were positive, the viral load was quantified by real-time polymerase chain reaction (RT-PCR). Other laboratory investigations included alanine aminotransferase, aspartate aminotransferase, albumin, and complete blood count. Only five children (2.71%) had HCV Ab positive with no other viral (A, B, and E) co-infections as determined by ELISA. Also, the RT-PCR detected HCV RNA in these ELISA positive children. The remaining children (179/184) were all negative for all hepatitis viruses' markers (HAV IgM, HEV IgM, HBs Ag, and HCV Ab). In conclusion, this study documented that, Cairo hospitals serving Egyptian children had a low prevalence of viral hepatitis (A, B, C, and E). More research with larger sample sizes from hospitals across Egypt is needed.","PeriodicalId":516584,"journal":{"name":"The Egyptian journal of immunology","volume":"28 5","pages":"30-39"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139640815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Serum microRNA-223 as a potential biomarker for allergic rhinitis and its correlation to eosinophil-derived neurotoxin. 血清 microRNA-223 作为过敏性鼻炎的潜在生物标志物及其与嗜酸性粒细胞衍生神经毒素的相关性

The Egyptian journal of immunology Pub Date : 2024-01-01 DOI: 10.55133/eji.310101

Fedaa Nabil, M. Alnemr, Sara F. Saadawy, Haytham K A Mahrous, Yasmin A Fahmy

{"title":"Serum microRNA-223 as a potential biomarker for allergic rhinitis and its correlation to eosinophil-derived neurotoxin.","authors":"Fedaa Nabil, M. Alnemr, Sara F. Saadawy, Haytham K A Mahrous, Yasmin A Fahmy","doi":"10.55133/eji.310101","DOIUrl":"https://doi.org/10.55133/eji.310101","url":null,"abstract":"Allergic rhinitis (AR) is a global health problem. It is an inflammatory condition defined by a malfunction of the immune system's regulatory mechanism. MicroRNA-223 (miRNA-223) has been linked to the modulation of AR in the last few years. The goal of this study was to determine whether miR-223 can be utilized as a potential biomarker for diagnosis of AR, and whether it correlates with the total nasal symptom score (TNSS) along with serum interleukin-17 (IL-17), interleukin-4 levels (IL-4) and eosinophil-derived neurotoxin (EDN). This study included 76 adult participants, consisted of 38 AR patients and 38 apparently healthy controls. Serum levels of miR-223 were assayed using real-time PCR. The levels of EDN, IL-17 and IL-4 in the serum were determined using an enzyme-linked immunosorbent assay. The optimal cutoff value for the analyzed factors to diagnose AR was determined using a receiver operating characteristic curve analysis (ROC). The demographic features (age and gender) of the two study groups were matched. Patients with pollen-induced AR had significantly higher levels of miR-223 in their serum compared to the controls (median = 3.82; median = 1.03, respectively, p < 0.001). In AR cases, a significant positive association was observed between miR-223 expression level and TNSS (r = 0.492, p = 0.002), EDN serum level (r = 0.427, p = 0.008), IL-4 serum level (r = 0.341, p = 0.036) and IL-17 serum level (r = 0.324, p = 0.047). MiR-223, at a cutoff value of 1.18, had a sensitivity and specificity of 94.9 % and 92.5%, respectively. In conclusion, miR-223 expression is significantly greater in blood of AR patients. There is a significant association between miR-223 and clinical severity of AR, each of IL-17 and IL-4 as well as EDN. Therefore, miR-223 may be employed as an effective biomarker for AR diagnosis.","PeriodicalId":516584,"journal":{"name":"The Egyptian journal of immunology","volume":"1 4","pages":"10-19"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139640868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Autoantibodies profile in autoimmune liver diseases and chronic viral hepatitis. 自身免疫性肝病和慢性病毒性肝炎的自身抗体谱。

The Egyptian journal of immunology Pub Date : 2024-01-01 DOI: 10.55133/eji.310107

R. Rabie, Alaa Hadhoud, Shymaa Abdelazim, Mohamed I Radwan, Marwa H Attia, Mohammed A Hasuna

{"title":"Autoantibodies profile in autoimmune liver diseases and chronic viral hepatitis.","authors":"R. Rabie, Alaa Hadhoud, Shymaa Abdelazim, Mohamed I Radwan, Marwa H Attia, Mohammed A Hasuna","doi":"10.55133/eji.310107","DOIUrl":"https://doi.org/10.55133/eji.310107","url":null,"abstract":"Despite their low prevalence, autoimmune liver diseases (AILD) cause liver cirrhosis, progress and leads to mortality from liver failure. Autoantibodies are confirmed to have significance in the early screening of AILD patients, especially in those who are asymptomatic before onset of clinical signs. This study aimed to assess levels of liver autoantibodies and their association with clinical manifestations of autoimmune liver diseases and chronic viral hepatitis (CVH) patients. This case-control study included 50 patients (case group of 25 patients with AILD and control group of 25 patients with CVH). They were investigated for presence of antibodies against LKM-1, AMA-M2, PML, M2-3E (BPO), gp210, Sp100, LC-1, Ro52 and SLA/LP using the line immune blot technique, and for the presence of antinuclear antibodies (ANA), as non-organ specific autoantibodies, using indirect immunofluorescence technique. Specific autoantibodies were detected in all AILD cases and some of their levels were significantly higher when compared with CVH group. Among AILD patients, 52% were positive for ANA, whereas 61.1% of chronic hepatitis C and 28.6% of chronic hepatitis B patients were positive for ANA with no significant difference (p=0.3). In conclusion, early diagnosis of autoimmune liver diseases has been linked to assessment of autoantibodies, allowing for prompt therapeutic intervention to stop the progression of liver cirrhosis and the accompanying complications.","PeriodicalId":516584,"journal":{"name":"The Egyptian journal of immunology","volume":"48 2","pages":"58-66"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139640844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Insight on the hepatitis B virus and host immune mechanisms in the context of occult hepatitis. 洞察隐匿性肝炎中的乙型肝炎病毒和宿主免疫机制。

The Egyptian journal of immunology Pub Date : 2024-01-01 DOI: 10.55133/eji.310110

Manal El Said, Asim M Alshanberi, Faten Abouelmagd, Mohammed Shaikhomer, Faten Abouelmagd

引用次数: 0