Current Biotechnology最新文献

{"title":"Conformational Dynamics of Post-Translational-Modified α-Synuclein\u0000(pY39 and pS87) and its Interaction with Lipid Membrane","authors":"D. Das, V. S. Mattaparthi","doi":"10.2174/0122115501310995240522064640","DOIUrl":"https://doi.org/10.2174/0122115501310995240522064640","url":null,"abstract":"\u0000\u0000The biological function of α-Synuclein (α-Syn), which includes controlling\u0000synaptic vesicles, is regulated by phosphorylation at the Tyrosine 39 (pY39) residue. This\u0000function can be important for both normal and aberrant functions, and it relies on the interaction\u0000of α-Syn with the lipid membrane. pY39 α-Syn is found to form morphologically distinct fibrils\u0000relative to wild-type (WT) α-Syn and shows less affinity to negatively charged vesicles. Also, the\u0000phosphorylation at position Serine 87 (pS87) is increased in synucleinopathies, which inhibits α-\u0000Syn oligomerization and affects the interaction between α-Syn and the membrane.\u0000\u0000\u0000\u0000This work aimed to study the effects of post-translation modifications of α-Syn (pY39\u0000and pS87) using all-atom Molecular Dynamics (MD) simulation\u0000\u0000\u0000\u0000In this computational study, we used all-atom MD simulations to investigate the effects\u0000of phosphorylation (pY39 and pS87) on protein-membrane interaction. The MD trajectories obtained\u0000were analyzed, and secondary structural content was calculated using YASARA software\u0000to perform a salt-bridge interaction study. Also, Principal component analysis was performed to\u0000analyze the secondary minima and global minima of the phosphorylated proteins.\u0000\u0000\u0000\u0000From the MD study, we observed that phosphorylation at the Tyr 39 position in α-Syn\u0000has a marked effect on its interaction with the lipid membrane. The conformational snapshots of\u0000α-Syn obtained showed a high degree of fluctuations in the N-terminal region that disrupts the helix-\u00002 binding region. The secondary structures of pS87 α-Syn were found to be retained and influence\u0000the NAC region to immerse into the membrane while inhibiting the potential to interact with\u0000other neighbouring molecules. Moreover, it was observed that in the case of pY39 α-Syn as opposed\u0000to pS87 α-Syn, there were larger energy disparities between the local and global minima of\u0000the overall structure.\u0000\u0000\u0000\u0000Therefore, disruption of the helix-2 binding region may affect the binding to the lipid\u0000membrane and take over interaction with other proteins or vesicles. In the case of pS87 α-Syn, the\u0000structure showed higher stability, but the NAC domain was found to emerge out of the membrane.\u0000","PeriodicalId":516136,"journal":{"name":"Current Biotechnology","volume":"252 6‐8","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141386654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytochemical Profiling and Molecular Investigation of Moringa Oleifera Lam. Leaves for Anti-Arthritic Potential: Assessment and Identification of Phytopharmaceuticals through GC-MS Analysis, In Silico Study, ADMET Analysis, and In Vitro Evaluation","authors":"Aanjaneya Mamgain, Rameshroo Kenwat, R. Paliwal","doi":"10.2174/0122115501304728240523052907","DOIUrl":"https://doi.org/10.2174/0122115501304728240523052907","url":null,"abstract":"\u0000\u0000The drumstick tree, Moringa oleifera Lam. (family Moringaceae), is known as a magical plant due to its broad pharmacological activities. Traditionally, the leaves of this plant are used for anti-inflammatory action. However, the compounds in Moringa oleifera leaves and their mechanism that showanti-arthritic potential are unknown.\u0000\u0000\u0000\u0000In this study, a preliminary phytochemical investigation of Moringa oleifera leaves eth-anolic extract was conductedusing qualitative analysis followed by Gas Chromatography-Mass Spectrometry (GC-MS) analysis to determine the constituents in the extracts.\u0000\u0000\u0000\u0000The results indicated the presence of various phytochemical compounds (about 316). Out of these, about 16 compounds were identified that covered 54.63 % of the total ethanolic extract. A molecular docking study was further performed using selected two compounds i.e. 3, 7, 11, 15-tetramethylhexadec-2-en-1-ol and neophytadiene and different targets proteins MMP9 (1L6J), PGE2 (1Z9H), TLR-1-TLR-2 (2Z80), COX-II (3NT1 and 5F19), iNOS (3NW2), HtrA1 (3TJO), JAK-1 (4K6Z), MCSF (5LXF) and TLR-4 (5NAO). Later on, an online tool was used to perform ADME/T analysis of the identified compounds. The DPPH and ABTS assay confirmed the strong potential of this extract for antioxidant activity, which correlates with anti-arthritic potential.\u0000\u0000\u0000\u0000Based on molecular docking, the mechanism for these compounds for the anti-arthritic activity of these magical plant leaves was identified. It is concluded from the study that Moringa oleifera leaves ethanolic extract have potential compounds that may be used to develop more ef-fective formulations for better therapeutic exercise against inflammatory diseases like rheumatoid arthritis.\u0000","PeriodicalId":516136,"journal":{"name":"Current Biotechnology","volume":"235 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141386868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic Impact of Ginger and Flaxseed in the Realm of Functional Foods","authors":"Dakshan Kumar N, A. Thirumurugan, S. Devarajan, T. Sathishkumar","doi":"10.2174/0122115501295239240409042621","DOIUrl":"https://doi.org/10.2174/0122115501295239240409042621","url":null,"abstract":"\u0000\u0000The efficacy of synthetic drugs in managing disease development and progression has\u0000faced challenges amidst shifts in lifestyle and heightened health consciousness. As a result, medicinal\u0000plants and seeds, with their constituent compounds, are gaining recognition for their potential\u0000in treating various illnesses. Ginger and flaxseed, as long-established herbal remedies, have made\u0000notable contributions to culinary and medicinal fields. Their robust flavor and aroma and anti-inflammatory,\u0000antioxidant, and analgesic properties extend their use beyond culinary spice to medicinal\u0000applications. Furthermore, their capacity to preserve food adds to their appeal, making ginger\u0000and flaxseed powerful and multi-functional elements in food production and medicinal contexts.\u0000This review highlights the therapeutic advantages of ginger and flaxseed in combating different\u0000diseases, focusing on their unique characteristics attributable to bioactive components.\u0000","PeriodicalId":516136,"journal":{"name":"Current Biotechnology","volume":" 19","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140689232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In silico Characterization of an Initiation Factor 2 Kinase of Black Fungi:\u0000A Potential Drug Target for Mycosis","authors":"C. B. Assunção, E. L. de Aguiar, Miguel Angel Chávez-Fumagalli, Emanuelle Rutren La Santrer, Sandro Renato Dias, Thiago de Souza Rodrigues, R. Caligiorne","doi":"10.2174/0122115501285434240409040348","DOIUrl":"https://doi.org/10.2174/0122115501285434240409040348","url":null,"abstract":"\u0000\u0000Fungi infections are responsible for more than 1.6 million deaths per year worldwide.\u0000Treatment is time-consuming, compromising the kidney and liver functions. in silico analyses\u0000have facilitated the discovery of new drugs that may present fewer side effects. In this connection,\u0000kinases that phosphorylate the translation initiation factor eIF-2 are candidate proteins for potent\u0000new drugs, which have been recognized as important in maintaining protein synthesis. Substances\u0000that interfere with the phosphorylation of the eIF2α factor may be the way to inhibit the production of proteins and accelerate the fungi's death. To determine whether this enzyme can be used as\u0000a new drug target, this study aimed to perform in silico functional annotation and characterization\u0000of eIF2 factor kinase´s three-dimensional structure from three species of black fungi. In addition,\u0000inhibitors that could interact and bind to the active site of the enzyme were explored. The hypothetical protein was submitted to the databases and bioinformatics tools for its characterization, whose\u0000analysis of protein-protein interactions was modeled and inhibitors anchored. Protein interaction\u0000analysis linked the kinases with other molecules in protein translation and ribosome recycling.\u0000However, centrality analysis showed only one kinase as a possible drug target. The inhibitors\u0000showed coupling with the active site of protein kinases, and these results indicate a possible blockade of the enzymatic function that can accelerate the response to the drugs. This study demonstrates that biochemical characterization and in silico validation studies of potential drugs can be\u0000more efficient and yield faster results.\u0000","PeriodicalId":516136,"journal":{"name":"Current Biotechnology","volume":" 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140685886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of Artificial Intelligence in Diabetic Wound Screening and Early\u0000Detection","authors":"Sanchit Dhankhar, Nitika Garg, Samrat Chauhan, Monika Saini","doi":"10.2174/0122115501303253240408072559","DOIUrl":"https://doi.org/10.2174/0122115501303253240408072559","url":null,"abstract":"\u0000\u0000The morbidity and death rates linked to diabetes mellitus are substantially increased by\u0000foot ulcers, a prevalent consequence of the disease. Proper wound management is essential for controlling foot ulcers. This includes monitoring the ulcers' healing progress through clinical reviews,\u0000changing dressings as needed, treating infections with the right medications, and ensuring that the\u0000ulcer is offloaded correctly. Taking pictures of the ulcer was a dependable way to track how diabetic foot ulcers were healing in the past. Images of foot ulcers have recently experienced a tremendous change due to the emergence of digital cameras in cell phones. Artificial intelligence\u0000(AI) and other recent developments in digital health technology present a great chance to improve\u0000the efficiency of diabetes care, which might reduce the growth in healthcare costs associated with\u0000diabetes. Patients with diabetes can alleviate the burden on clinics and patients' transportation demands by electronically sharing photos of their ulcers, which diabetes care providers can remotely\u0000monitor. Improved remote monitoring of diabetic foot ulcers using smartphone apps is now possible with the help of a new generation of AI-powered solutions. This clinical update review aims to\u0000gather information on this trending topic so that medical professionals can be current on all the latest advancements in the field.\u0000","PeriodicalId":516136,"journal":{"name":"Current Biotechnology","volume":"35 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140699744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Navigating the Regulatory Landscape of Genetically Modified Foods in\u0000India: An Update","authors":"Gaurav, Animesh Ranjan, N. S. Patil, R. Narang, Amandeep Singh","doi":"10.2174/0122115501279523231227041835","DOIUrl":"https://doi.org/10.2174/0122115501279523231227041835","url":null,"abstract":"<jats:sec>\u0000<jats:title />\u0000<jats:p />\u0000</jats:sec>","PeriodicalId":516136,"journal":{"name":"Current Biotechnology","volume":"67 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139450258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}