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Genotypic variability and physio-morphological efficiency of buckwheat (Fagopyrum spp.) under moisture stress at mid-altitudes of Meghalaya (India) 梅加拉亚(印度)中海拔地区水分胁迫下荞麦(Fagopyrum spp.)的基因型变异和生理形态效率
IF 1.9 4区 农林科学
Crop & Pasture Science Pub Date : 2022-09-13 DOI: 10.1071/CP22062
S. Hajong, Krishnappa Rangappa, Harish Ganjalagatta Dasaiah, P. Moirangthem, U. S. Saikia, B. Bhattacharjee, S. Ahlawat, B. Kandpal
{"title":"Genotypic variability and physio-morphological efficiency of buckwheat (Fagopyrum spp.) under moisture stress at mid-altitudes of Meghalaya (India)","authors":"S. Hajong, Krishnappa Rangappa, Harish Ganjalagatta Dasaiah, P. Moirangthem, U. S. Saikia, B. Bhattacharjee, S. Ahlawat, B. Kandpal","doi":"10.1071/CP22062","DOIUrl":"https://doi.org/10.1071/CP22062","url":null,"abstract":"Abstract Context. Crop production in Eastern Himalaya is constrained by combined effects of soil acidity and moisture deficit during post monsoon under a changing climate. Aim. This study aimed to identify potential buckwheat genotypes and stress mechanisms in hill environments of Meghalaya (India). Method. Field and microcosm experiments were conducted to study field performance, genotypic variability and physio-morphological efficiency in buckwheat. Key results. Substantial genetic variability for shoot length (30.3–110 cm), crop canopy distribution (3.0–7.5 number of primary branches), leaf area retention (15.7–60.2 cm2/two leaves), 1000 seed weight (16.2–34.7 g) and seed yield (0.088–1.31 metric tonne (MT)/ha) was observed. Mean grain yield of buckwheat genotypes was 0.446 MT/ha whereas IC13411, IC24298 and IC37305 produced significantly higher seed yield (1.31, 1.28 and 1.14 MT/ha, respectively) accounting to 1.93, 1.87 and 1.67 times higher than average yield (0.680 MT/ha). Genotypes like IC42416, EC323730 and EC218784 have lower crop yields (0.8, 1.02, 0.93 MT/ha respectively). Interestingly, few high yielders (IC13411 and IC24298) exhibited higher photosynthetic rate (46.1 and 32.3 μmol/m2.s), increased stomatal conductance (311.0 and 326.2 mmol/m2.s), leaf chlorophyll (2.47 and 2.55 mg/g fresh weight (FW)) and carotenoids (95 and 106.3 μg/g FW). Chlorophyll a and b ratio (3.3 and 3.0) and total chlorophyll to carotenoids (4.2 and 4.0) were higher in high yielders (IC13411 and IC24298) compared to other low yielders. Conclusions. Robust root systems, stomatal structure and higher cuticle wax ideally enhanced water use efficiency (WUE) and drought tolerance in high-yielding buckwheat genotypes. Implications. IC13411 and IC24298 are promising for enhanced productivity in the mountainous ecosystem of Meghalaya.","PeriodicalId":51237,"journal":{"name":"Crop & Pasture Science","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2022-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49257507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Silicon-mediated improvement of biomass yield and physio-biochemical attributes in heat-stressed spinach (Spinacia oleracea) 硅对热胁迫菠菜生物量产量和生理生化特性的改善
IF 1.9 4区 农林科学
Crop & Pasture Science Pub Date : 2022-09-06 DOI: 10.1071/CP22192
Quratulain Khosa, Q. Zaman, Tingting An, Kamran Ashraf, A. Abbasi, S. Nazir, Riffat Naz, Yinglong Chen
{"title":"Silicon-mediated improvement of biomass yield and physio-biochemical attributes in heat-stressed spinach (Spinacia oleracea)","authors":"Quratulain Khosa, Q. Zaman, Tingting An, Kamran Ashraf, A. Abbasi, S. Nazir, Riffat Naz, Yinglong Chen","doi":"10.1071/CP22192","DOIUrl":"https://doi.org/10.1071/CP22192","url":null,"abstract":"Abstract Context. Defensive action of plants against biotic and abiotic stresses has been augmented by silicon (Si). Spinach (Spinacia oleracea L.) is a nutritious leafy vegetable that is a cold-tolerant but heat-sensitive crop. Aims and methods. The ability of exogenous application of Si (0, 2, 4 and 6 mmol L−1 in the form of K2SiO3) to alleviate heat stress in spinach cv. Desi Palak was investigated. After an acclimatisation period, plants were grown with or without heat stress for 15 days, followed by Si treatment for 25 days. Plant growth and physiology were assessed at 65 days after sowing. Key results. Heat stress significantly inhibited plant growth, water status and photosynthesis, soluble sugar and protein contents, and osmolyte status in spinach leaves, but increased electrolyte leakage, activities of antioxidant enzymes, and proline content. Application of Si alleviated heat stress by enhancing water status, photosynthetic pigments, soluble proteins and essential minerals, and by reducing damage of the plasma membrane. The accumulation of osmolytes counterbalance the osmotic stress imposed by heat. Conclusions. Silicon plays a vital role in alleviating effects of heat stress by improving water status and photosynthetic rate, and accruing osmoprotectants other than proline. Implications. Exogenous application of Si is an efficient strategy to boost tolerance of spinach plants to heat stress, having significant impact on growth and productivity of spinach at higher temperatures.","PeriodicalId":51237,"journal":{"name":"Crop & Pasture Science","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2022-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46710172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Can pasture defoliation management targets be similar under full sun and shaded conditions? 在阳光充足和遮荫的条件下,牧场落叶管理目标是否相似?
IF 1.9 4区 农林科学
Crop & Pasture Science Pub Date : 2022-08-31 DOI: 10.1071/CP21592
Gustavo Krahl, T. C. Baldissera, C. E. Pinto, F. C. Garagorry, S. Werner, C. F. Lopes, H. R. Ribeiro Filho
{"title":"Can pasture defoliation management targets be similar under full sun and shaded conditions?","authors":"Gustavo Krahl, T. C. Baldissera, C. E. Pinto, F. C. Garagorry, S. Werner, C. F. Lopes, H. R. Ribeiro Filho","doi":"10.1071/CP21592","DOIUrl":"https://doi.org/10.1071/CP21592","url":null,"abstract":"Abstract Context. Defoliation management targets applied to forages under the full sun have not yet been properly evaluated for shaded environments such as occur in integrated crop–livestock systems with the presence of trees. AimsThis study aimed to determine defoliation targets under full sun and shaded environments for the shade-tolerant perennial summer grass hybrid Axonopus catharinensis (giant missionary grass), widely used in pasture systems of South Brazil and Argentina. Methods. Four pre-defoliation canopy heights (15, 25, 35 and 45 cm) and four defoliation severities (20%, 40%, 60% and 80% reduction in pre-defoliation canopy height) were evaluated. Plants were grown in 0.15 m3 wooden boxes filled with sand/vermiculite substrate (1:1 v/v) and irrigated with a complete nutrient solution. An artificial shade structure was made with wooden slats, reducing light intensity by 50% for shaded plants. Key results. Herbage accumulation decreased by 54% in shaded plants compared with those under full sun. Regardless of the light environment, the lowest crude protein content (<150 g/kg dry matter) and the highest neutral detergent fibre content (>650 g/kg dry matter) were observed when the defoliation target height was >35 cm. Both shaded and full sun environments showed decreases in tiller density when defoliation severity was >60% of pre-defoliation canopy heights. Conclusions. Pre-defoliation canopy heights >35 cm and defoliation severity >60% of pre-defoliation canopy height should be avoided when managing A. catharinensis, regardless of the light environment. Implications. Recommended targets may be easily implemented by livestock producers and are similar for full sun and shade environments.","PeriodicalId":51237,"journal":{"name":"Crop & Pasture Science","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2022-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47452040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Random regression models for multi-environment, multi-time data from crop breeding selection trials 作物育种试验多环境、多时间数据的随机回归模型
IF 1.9 4区 农林科学
Crop & Pasture Science Pub Date : 2022-08-31 DOI: 10.1071/CP21732
J. De Faveri, A. Verbyla, G. Rebetzke
{"title":"Random regression models for multi-environment, multi-time data from crop breeding selection trials","authors":"J. De Faveri, A. Verbyla, G. Rebetzke","doi":"10.1071/CP21732","DOIUrl":"https://doi.org/10.1071/CP21732","url":null,"abstract":"ABSTRACT Context. In order to identify best crop genotypes for recommendation to breeders, and ultimately for use in breeding, evaluation is usually conducted in field trials across a range of environments, known as multi-environment trials. Increasingly, many breeding traits are measured over time, for example with high-throughput phenotyping at different growth stages in annual crops or repeated harvests in perennial crops. Aims. This study aims to provide an efficient, accurate approach for modelling genotype response over time and across environments, accounting for non-genetic sources of variation such as spatial and temporal correlation. Methods. Because the aim is genotype selection, genetic effects are fitted as random effects, and so the approach is based on random regression, in which linear or non-linear models are used to model genotype responses. A method for fitting random regression is outlined in a multi-environment situation, using underlying cubic smoothing splines to model the mean trend over time. This approach is illustrated on six wheat experiments, using data on grain-filling over thermal time. Key results. The method correlates genetic effects over time and environments, providing predicted genotype responses while incorporating spatial and temporal correlation between observations. Conclusions. The approach provides robust genotype predictions by accounting for temporal and spatial effects simultaneously under various situations including those in which trials have different measurement times or where genotypes within trials are not measured at the same times. The approach facilitates investigation into genotype by environment interaction (G × E) both within and across environments. Implications. The models presented have potential to increase accuracy of predictions over measurement times and trials, provide predictions at times other than those observed, and give a greater understanding of G × E interaction, hence improving genotype selection across environments for repeated-measures traits.","PeriodicalId":51237,"journal":{"name":"Crop & Pasture Science","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2022-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41547098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Assessment of genetic diversity and DNA fingerprinting of rare species of the genus Crambe (Brassicaceae) 十字花属稀有物种的遗传多样性评价及DNA指纹图谱
IF 1.9 4区 农林科学
Crop & Pasture Science Pub Date : 2022-08-31 DOI: 10.1071/CP22027
V. Chokheli, A. Shmaraeva, I. Kornienko, V. V. Stepanenko, V. Rajput, Abhishek Singh, A. Kumari, P. Dmitriev, A. S. Azarov, T. Minkina, T. Varduni
{"title":"Assessment of genetic diversity and DNA fingerprinting of rare species of the genus Crambe (Brassicaceae)","authors":"V. Chokheli, A. Shmaraeva, I. Kornienko, V. V. Stepanenko, V. Rajput, Abhishek Singh, A. Kumari, P. Dmitriev, A. S. Azarov, T. Minkina, T. Varduni","doi":"10.1071/CP22027","DOIUrl":"https://doi.org/10.1071/CP22027","url":null,"abstract":"ABSTRACT Context. Preservation of genetic diversity of species is a pressing issue; in particular, the preservation of the gene pool of many rare and endangered plants is a priority. Species of the genus Crambe (family Brassicaceae) have potential in agriculture and bioremediation. Aims. This study aims to assess the genetic diversity and DNA fingerprint of five rare species of the genus Crambe, and to develop the concept of a genetic passport for rare plants. Methods. DNA fingerprinting was conducted via the inter simple sequence repeat (ISSR) method, using six ISSR primers. Hierarchical cluster analysis of the species was performed. Key results. In total, 145 polymorphic loci were detected. The studied species formed two different clusters on genetic dendrogram analysis. The first cluster comprised two species, Crambe steveniana and C. tataria from a single subsection (Tatariae), forming a common clade. They were the most genetically close species with genetic similarity of 0.8431. The remaining three species (C. cordifolia, C. maritima, C. pinnatifida), from another subsection of Crambe, formed nodes from the general clade. Conclusions. The findings allow plants to be genetically certified using our methods and informative ISSR primers for rare plants. We were able to propose a genetic formula identifying a species and present an example of a genetic passport for rare plants. The genetic structure of C. pinnatifida was investigated for the first time. Implications. The present findings contribute to the systematics and critical investigation of rare plant species with genetic resource potential. A genetic passport could be used in the protection of copyright of agricultural varieties.","PeriodicalId":51237,"journal":{"name":"Crop & Pasture Science","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2022-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42842102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Current status of and future opportunities for digital agriculture in Australia 澳大利亚数字农业的现状和未来机遇
IF 1.9 4区 农林科学
Crop & Pasture Science Pub Date : 2022-08-29 DOI: 10.1071/CP21594
B. D. Hansen, E. Leonard, M. C. Mitchell, J. Easton, N. Shariati, M. Mortlock, M. Schaefer, D. Lamb
{"title":"Current status of and future opportunities for digital agriculture in Australia","authors":"B. D. Hansen, E. Leonard, M. C. Mitchell, J. Easton, N. Shariati, M. Mortlock, M. Schaefer, D. Lamb","doi":"10.1071/CP21594","DOIUrl":"https://doi.org/10.1071/CP21594","url":null,"abstract":"ABSTRACT In Australia, digital agriculture is considered immature and its adoption ad hoc, despite a relatively advanced technology innovation sector. In this review, we focus on the technical, governance and social factors of digital adoption that have created a disconnect between technology development and the end user community (farmers and their advisors). Using examples that reflect both successes and barriers in Australian agriculture, we first explore the current enabling technologies and processes, and then we highlight some of the key socio-technical factors that explain why digital agriculture is immature and ad hoc. Pronounced issues include fragmentation of the innovation system (and digital tools), and a lack of enabling legislation and policy to support technology deployment. To overcome such issues and increase adoption, clear value propositions for change are necessary. These value propositions are influenced by the perceptions and aspirations of individuals, the delivery of digitally-enabled processes and the supporting legislative, policy and educational structures, better use/conversion of data generated through technology applications to knowledge for supporting decision making, and the suitability of the technology. Agronomists and early adopter farmers will play a significant role in closing the technology-end user gap, and will need support and training from technology service providers, government bodies and peer-networks. Ultimately, practice change will only be achieved through mutual understanding, ownership and trust. This will occur when farmers and their advisors are an integral part of the entire digital innovation system.","PeriodicalId":51237,"journal":{"name":"Crop & Pasture Science","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2022-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45633703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 9
Intraspecific genetic variation among Sehima nervosum genotypes in relation to agro-climatic diversity 麻豆基因型种内遗传变异与农业气候多样性的关系
IF 1.9 4区 农林科学
Crop & Pasture Science Pub Date : 2022-08-15 DOI: 10.1071/CP22057
A. Roy, D. Malaviya, P. Kaushal, S. K. Mahanta, R. Tewari, R. Chauhan, A. Chandra
{"title":"Intraspecific genetic variation among Sehima nervosum genotypes in relation to agro-climatic diversity","authors":"A. Roy, D. Malaviya, P. Kaushal, S. K. Mahanta, R. Tewari, R. Chauhan, A. Chandra","doi":"10.1071/CP22057","DOIUrl":"https://doi.org/10.1071/CP22057","url":null,"abstract":"Abstract Context. The genus Sehima is an important component of grasslands worldwide and is the dominant grass of Sehima–Dichanthium grasslands of India. It is an excellent fodder and a good source of lignocellulosic material and several industrially important biomolecules. Aims. We aimed to characterise and conserve locally adapted diverse germplasm of S. nervosum, collected from different agro-climatic zones, for effective utilisation. Methods. Morphological and nutritive traits were measured and statistically analysed for diversity in a germplasm collection of S. nervosum genotypes from diverse agro-climatic conditions of India, and a representative core subset was created. Key results. Morphological traits and nutritive parameters such as crude protein content and digestibility showed wide variability among accessions. Principal component analysis established that plant height, number of tillers per tussock and number of nodes per tiller accounted for half of the variation present. Tiller internode length, stem diameter, and leaf blade length and width also contributed >5 unit points each to the cumulative proportion of variance accounted for. Conclusions. The set of germplasm, possessing substantial variability, is a valuable genetic resource for developing new cultivars. Genotypes identified with high protein content could be used as forage, and those with high cellulose and hemicelluloses as a biofuel resource. Implications. Genotypes can be separately identified for forage and biofuel, and the core subset can effectively be used for evaluation and selection of genotypes in target environments. Additionally, the core subset can be used to establish and rejuvenate sustainable pastures by allowing natural selection.","PeriodicalId":51237,"journal":{"name":"Crop & Pasture Science","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2022-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41502377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Biochar increases soil enzyme activities in two contrasting pastoral soils under different grazing management 在两种不同的放牧管理下,生物炭提高了土壤酶活性
IF 1.9 4区 农林科学
Crop & Pasture Science Pub Date : 2022-08-12 DOI: 10.1071/CP21790
S. Garbuz, A. Mackay, M. Camps-Arbestain, B. Devantier, M. Minor
{"title":"Biochar increases soil enzyme activities in two contrasting pastoral soils under different grazing management","authors":"S. Garbuz, A. Mackay, M. Camps-Arbestain, B. Devantier, M. Minor","doi":"10.1071/CP21790","DOIUrl":"https://doi.org/10.1071/CP21790","url":null,"abstract":"ABSTRACT Context. Soil enzyme activities are key regulators of carbon and nutrient cycling in grazed pastures. Aims. We investigated the effect of biochar addition on the activity of seven enzymes involved in the carbon, nitrogen and phosphorus cycles in a Sil-andic Andosol and a Dystric Cambisol under permanent pastures. Methods. The study consisted of a one-year field-based mesocosm experiment involving four pastures under different nutrient and livestock practices: with and without effluent under dairy cow grazing on the Andosol, and with either nil or high phosphorus fertiliser input under sheep grazing on the Cambisol. Soil treatments were: (1) willow biochar added at 1% w/w; (2) lime added at the liming equivalence of biochar (positive control); (3) no amendments (negative control). Key results. Compared with the Cambisol, the Andosol had higher dehydrogenase, urease, alkaline and acid phosphatase and, especially, nitrate-reductase activities, aligning with its higher pH and fertility. In both soils, biochar addition increased the activity of all enzymes, except for acid phosphatase and peroxidase; lime addition increased peroxidase and nitrate-reductase activity. Conclusions. The increased enzyme activity was strongly positively correlated with soil biological activity following biochar addition. Biochar caused a 40–45% increase in cellulase activity, attributed to increased root biomass following biochar addition. The response in acid and alkaline phosphatase activity can be attributed to the impact of biochar and lime addition on soil pH. Implications. The results provide more insights in realising the potential benefits of biochar to the provision of ecosystem services for grazed pastures.","PeriodicalId":51237,"journal":{"name":"Crop & Pasture Science","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2022-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41851013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
Comparative analysis of nutritional composition of seeds of wild-type soybean and lines overexpressing GmWRI1a 野生型大豆与过表达GmWRI1a系种子营养成分的比较分析
IF 1.9 4区 农林科学
Crop & Pasture Science Pub Date : 2022-08-08 DOI: 10.1071/CP22161
Mingming Yang, Chao Yang, Qingyan Zhang, Meng Li, Yuanzhuo Wang, Pin Shang, Baizheng Ren, Zhaohui Zuo, Siqi Xing, W. Teng, Zhikun Wang
{"title":"Comparative analysis of nutritional composition of seeds of wild-type soybean and lines overexpressing GmWRI1a","authors":"Mingming Yang, Chao Yang, Qingyan Zhang, Meng Li, Yuanzhuo Wang, Pin Shang, Baizheng Ren, Zhaohui Zuo, Siqi Xing, W. Teng, Zhikun Wang","doi":"10.1071/CP22161","DOIUrl":"https://doi.org/10.1071/CP22161","url":null,"abstract":"ABSTRACT Context. With the widespread use of transgenic soybeans (Glycine max (L. ) Merr.), their nutritional assessment for human food and animal feed is an important aspect of safety evaluation. WRINKLED 1 (WRI1) is a transcriptional activator of genes involved in fatty acid synthesis. Aims. We aimed to evaluate the effect of overexpression of GmWRI1a in soybean on the nutritional profile of soybean seeds. Methods. Using molecular biology techniques, we identified three transgenic soybean lines stably overexpressing GmWRI1a (GmWRI1a-OE). We quantified the nutritional and anti-nutritional components in seeds of GmWRI1a-OE and wild-type (DN50) soybean, and compared them on the basis of the principle of substantial equivalence. Key results. The GmWRI1a and Bar genes were stably inherited in the three GmWRI1a-OE lines, and GmWRI1a protein content was higher in transgenic soybean seeds than in wild-type seeds. Overexpression of GmWRI1a resulted in changes in fatty acid composition, a significant increase in oil content, and a significant decrease in stachyose content in seeds. Contents of other nutritional components (proximates, amino acids and isoflavones) and anti-nutritional factors (phytic acid, trypsin inhibitors and raffinose) in seeds were not significantly different between the wild-type and GmWRI1a-OE lines. Conclusions. Contents of nutritional and anti-nutritional components of GmWRI1a-OE seeds were all within the reference ranges reported for commercially available soybeans and, therefore, are substantially equivalent to those of wild-type seeds. Implications. GmWRI1a-OE soybean seeds are a high-quality product for health-conscious consumers.","PeriodicalId":51237,"journal":{"name":"Crop & Pasture Science","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2022-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41655601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of biochar doses under various levels of salt stress on soil nutrient availability, soil enzyme activities and plant growth in a marigold crop 不同盐胁迫水平下生物炭用量对金盏花作物土壤养分有效性、土壤酶活性和植物生长的影响
IF 1.9 4区 农林科学
Crop & Pasture Science Pub Date : 2022-07-30 DOI: 10.1071/CP21542
R. Premalatha, P. Malarvizhi, E. Parameswari
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引用次数: 6
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