Biochemistry and Molecular Biology最新文献

Comparison of the Loop-Mediated Isothermal Amplification (LAMP) and the Kato-Katz Techniques in the Diagnosis of Schistosoma mansoni in Burkina Faso 环路介导等温扩增（LAMP）和卡托-卡茨技术在布基纳法索曼氏血吸虫诊断中的比较

Biochemistry and Molecular Biology Pub Date : 2024-06-03 DOI: 10.11648/j.bmb.20240902.11

Farida C A Kaboré, Salif Sombie, B. Bayala, Harouna Sorè, Nicolas Ouedraogo, Henri Some, Aristide Ouattara, Liz Coulibaly, I. Soulama, Jacques Simpore

{"title":"Comparison of the Loop-Mediated Isothermal Amplification (LAMP) and the Kato-Katz Techniques in the Diagnosis of Schistosoma mansoni in Burkina Faso","authors":"Farida C A Kaboré, Salif Sombie, B. Bayala, Harouna Sorè, Nicolas Ouedraogo, Henri Some, Aristide Ouattara, Liz Coulibaly, I. Soulama, Jacques Simpore","doi":"10.11648/j.bmb.20240902.11","DOIUrl":"https://doi.org/10.11648/j.bmb.20240902.11","url":null,"abstract":"Intestinal schistosomiasis or intestinal bilharzia, mainly caused by Schistosoma mansoni, is one of the most common parasitic diseases in the world, and a neglected tropical disease (NTD). It ranks first among water-borne diseases and is the 2nd most endemic parasitic disease after malaria and according to the World Health Organization (WHO), schistosomiasis is transmitted in more than 78 countries and territories in tropical and subtropical regions, and more than 250 million people are infected, mainly in Africa. Kato Katz (KK) remains the standard technique for diagnosing this disease. A promising new approach, loop-mediated isothermal amplification (LAMP), may be needed in developing countries such as Burkina Faso. Thus, the aim of this study was to compare the LAMP technique and the Kato-Katz technique in the diagnosis of Schistosoma mansoni in Burkina Faso. 52 stool samples were collected from patients in the town of Bobo Dioulasso and examined using the KK technique, which corresponds to microscopy and the LAMP technique, to assess the sensitivity and specificity of this molecular technique. The results showed a prevalence of intestinal schistosomiasis of 8% in the study, and the Kappa coefficient obtained between the 2 techniques was 0.99, roughly equal to 1. The sensitivity and specificity of the LAMP molecular test was 100%.\u0000","PeriodicalId":511384,"journal":{"name":"Biochemistry and Molecular Biology","volume":"18 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141389018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Highly Active Antiretroviral Treatment of HIV-1 Patients, Molecular Evaluation of AZT+3TC+EFV Regim HIV-1 患者的高活性抗逆转录病毒治疗，AZT+3TC+EFV 方案的分子评估

Biochemistry and Molecular Biology Pub Date : 2024-01-18 DOI: 10.11648/j.bmb.20240901.14

Babacar Faye, Bineta Ndiaye, Cheikh Madické Ndiaye, Mame Diarra Bousso Lam, Ismaïl Barkiré, Micailou Magassouba, Cathy Cissé, Aissatou Ngom, Alioune Dièye

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Evaluation of Rhizobia Strain of Soybean (G. max) for Symbiotic Performance Grown in Acid-Prone Areas 评估酸性地区大豆（G. max）根瘤菌株的共生性能

Biochemistry and Molecular Biology Pub Date : 2024-01-11 DOI: 10.11648/j.bmb.20240901.13

Mamo Bekele, G. Yilma

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Effect of Biochar Application on Early Germination Rate of Water Melon Seeds 施用生物炭对西甜瓜种子早期发芽率的影响

Biochemistry and Molecular Biology Pub Date : 2024-01-08 DOI: 10.11648/j.bmb.20240901.12

Opara Ifeoma Juliet, Ukoha Pius Oziri, Obasi Nnamdi Lawrence, Ekere Nwachukwu

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Evaluating Gene Expression and Signaling Activities in Lipopolysaccharide Induced Human Peripheral Blood Mononuclear Cells 评估脂多糖诱导的人类外周血单核细胞的基因表达和信号活动

Biochemistry and Molecular Biology Pub Date : 2024-01-08 DOI: 10.11648/j.bmb.20240901.11

Kristin Zindel, Sarah Whiteman, Kurstin Wiewel, A. Turner, Meghan McGivern, Suzanne Stapleton, M. Jett, C. Mendis

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