Comparison of the Loop-Mediated Isothermal Amplification (LAMP) and the Kato-Katz Techniques in the Diagnosis of Schistosoma mansoni in Burkina Faso

Abstract

Intestinal schistosomiasis or intestinal bilharzia, mainly caused by Schistosoma mansoni, is one of the most common parasitic diseases in the world, and a neglected tropical disease (NTD). It ranks first among water-borne diseases and is the 2nd most endemic parasitic disease after malaria and according to the World Health Organization (WHO), schistosomiasis is transmitted in more than 78 countries and territories in tropical and subtropical regions, and more than 250 million people are infected, mainly in Africa. Kato Katz (KK) remains the standard technique for diagnosing this disease. A promising new approach, loop-mediated isothermal amplification (LAMP), may be needed in developing countries such as Burkina Faso. Thus, the aim of this study was to compare the LAMP technique and the Kato-Katz technique in the diagnosis of Schistosoma mansoni in Burkina Faso. 52 stool samples were collected from patients in the town of Bobo Dioulasso and examined using the KK technique, which corresponds to microscopy and the LAMP technique, to assess the sensitivity and specificity of this molecular technique. The results showed a prevalence of intestinal schistosomiasis of 8% in the study, and the Kappa coefficient obtained between the 2 techniques was 0.99, roughly equal to 1. The sensitivity and specificity of the LAMP molecular test was 100%.