Journal of Advances in Microbiology最新文献

Antifungal Activity of Jatropha curcas Seed Extracts Analysed by GC-MS against Two Cowpea (Vigna unguiculata L.) Pathogenic Fungi 利用气相色谱-质谱分析麻风树籽提取物对两种豇豆（Vigna unguiculata L.）病原真菌的抗真菌活性

Journal of Advances in Microbiology Pub Date : 2024-07-26 DOI: 10.9734/jamb/2024/v24i7841

Tchasep Wandji Nadège, N. Patrice, Kuate Tueguem Nobert William, Tize Tize, Atindo Songwe Thierry, Ndongo Biyo’o Eric, Ngata Ngadjui Laurence, G. G. F. Christian, N. Bekolo

{"title":"Antifungal Activity of Jatropha curcas Seed Extracts Analysed by GC-MS against Two Cowpea (Vigna unguiculata L.) Pathogenic Fungi","authors":"Tchasep Wandji Nadège, N. Patrice, Kuate Tueguem Nobert William, Tize Tize, Atindo Songwe Thierry, Ndongo Biyo’o Eric, Ngata Ngadjui Laurence, G. G. F. Christian, N. Bekolo","doi":"10.9734/jamb/2024/v24i7841","DOIUrl":"https://doi.org/10.9734/jamb/2024/v24i7841","url":null,"abstract":"Cowpea (Vigna unguiculata L. Walp.), native to Africa, is one of the most popular and widely grown seed legumes in arid and semi-arid areas. In these areas, fusarium wilt and white rots caused by Fusarium oxysporum Schl. f.sp. tracheiphilum and Sclerotinia sclerotiorum are the most devastating pathogens of cowpea crops, causing losses of 50-100 %, respectively. This study aims to evaluate the antifungal potential of biochemical compounds in Jatropha curcas L. seed extracts identified by GC-MS analysis against F. oxysporum and S. sclerotiorum. In vitro tests were carried out using aqueous, acetone and hexane extracts of J. curcas seeds at concentrations of 15, 30, 60 and 120 μL/mL and a synthetic fungicide (3.33 g/L). Mycelial growth and Minimal Inhibitory Concentrations (MIC50) were assessed. As a result, J. curcas seed extracts are rich in phytochemical molecules such as 9,12-octadecadienoic acid (Z,Z) ; n-Hexadecanoic acid ; Squalene ; D-Limonene ; 9,15-octadecadienoic acid, methy and 1,3-Dioxane, 5-ethyl-2,2-dimethyl, with antifungal activity. Total inhibition (100%) of mycelial growth of F. oxysporum and S. sclerotiorum was obtained in Petri dishes contaminated with the aqueous and acetone extracts at concentrations C3=60 µL/mL and C4=120 µL/mL, as well as with the synthetic fungicide. The MIC50 obtained with isolates of S. sclerotiorum and F. oxysporum were 9.2 and 9.96 µL/mL with the aqueous extract, respectively followed by the acetone extract (12.96 and 14.97 µL/mL). All Jatropha curcas lethal extracts tested were fungistatic, but the synthetic fungicide was fungicidal. Bioformulation based on J. curcas and characterization of pathogen are needed.","PeriodicalId":510775,"journal":{"name":"Journal of Advances in Microbiology","volume":"26 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141801529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of the Consistency of MALDI-TOF Results with Traditional Methods for the Identification of Urease-producing Enterobacteriaceae in Wastewater from Abidjan, Côte d'Ivoire 评估 MALDI-TOF 与传统方法鉴定科特迪瓦阿比让废水中产尿素酶肠杆菌科细菌结果的一致性

Journal of Advances in Microbiology Pub Date : 2024-07-18 DOI: 10.9734/jamb/2024/v24i7840

Tetty Aya Marie Pascale, Gnali Gbohounou Fabrice, Yao Djeha Rosine, Y. Eric, Dosso Mireille

{"title":"Evaluation of the Consistency of MALDI-TOF Results with Traditional Methods for the Identification of Urease-producing Enterobacteriaceae in Wastewater from Abidjan, Côte d'Ivoire","authors":"Tetty Aya Marie Pascale, Gnali Gbohounou Fabrice, Yao Djeha Rosine, Y. Eric, Dosso Mireille","doi":"10.9734/jamb/2024/v24i7840","DOIUrl":"https://doi.org/10.9734/jamb/2024/v24i7840","url":null,"abstract":"The aim of this study was to evaluate the concordance between Matrix-Assisted Laser Desorption/Ionization-Time of Flight (MALDI-TOF) mass spectrometry and traditional biochemical methods for identifying urease-producing Enterobacteria in wastewater samples. This research was a comparative study assessing the efficacy of MALDI-TOF mass spectrometry versus traditional biochemical methods for bacterial identification. The study was conducted in Abidjan over a period of five weeks. A total of 50 wastewater samples were collected for analysis. The samples underwent a two-step enrichment process: initial pre-enrichment in EPT broth at 37°C for 18-24 hours, followed by enrichment in RV10 broth at 42°C for 24 hours. Subsequently, isolates were cultured on Hektoen agar, observed for specific colony characteristics, and subjected to the urease test. Final identification was performed using MALDI-TOF mass spectrometry. Of the 50 samples, 46 produced identifiable colonies. The distribution of identified species was as follows: Proteus mirabilis in 22 samples (47.82%), Providencia stuartii in 16 samples (34.78%), and Klebsiella pneumoniae in 8 samples (17.39%). MALDI-TOF mass spectrometry demonstrated over 90% concordance with traditional biochemical methods, accurately identifying bacterial species with high precision. MALDI-TOF mass spectrometry proved to be a rapid, precise, and efficient method for identifying urease-producing Enterobacteria in wastewater. Its high concordance with traditional methods suggests its potential to replace conventional techniques in microbiological laboratories, enhancing the speed and accuracy of bacterial identification in water quality management.","PeriodicalId":510775,"journal":{"name":"Journal of Advances in Microbiology","volume":" 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141827250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chemical Quality of Moringa Paneer Prepared from Buffalo Milk 水牛奶制备的辣木奶酪的化学质量

Journal of Advances in Microbiology Pub Date : 2024-07-17 DOI: 10.9734/jamb/2024/v24i7839

Vikash Kumar, M.P.S. Yadav, Kuldeep, Sandeep Kumar

引用次数: 0

In vitro Development of Two Alternaria solani Strains, Causal Agent of Alternariose in Tomato (Lycopersicon esculentum) under the Influence of Thevetia peruviana Seeds Extracts 在 Thevetia peruviana 种子提取物的影响下番茄（Lycopersicon esculentum）交链孢属（Alternaria solani）病原菌两种菌株的体外发育情况

Journal of Advances in Microbiology Pub Date : 2024-07-10 DOI: 10.9734/jamb/2024/v24i7838

L. N. Ngata, E. L. M. Ngonkeu, A. Ndogho, C. K. Ntongo, F. C. Gbaporo, P. Z. Ngatsi, S. L. L. Dida, L. T. V. Dongmo, T. S. Atindo, W. Kuaté, Z. Ambang

{"title":"In vitro Development of Two Alternaria solani Strains, Causal Agent of Alternariose in Tomato (Lycopersicon esculentum) under the Influence of Thevetia peruviana Seeds Extracts","authors":"L. N. Ngata, E. L. M. Ngonkeu, A. Ndogho, C. K. Ntongo, F. C. Gbaporo, P. Z. Ngatsi, S. L. L. Dida, L. T. V. Dongmo, T. S. Atindo, W. Kuaté, Z. Ambang","doi":"10.9734/jamb/2024/v24i7838","DOIUrl":"https://doi.org/10.9734/jamb/2024/v24i7838","url":null,"abstract":"Alternaria solani is a fungus that causes yield losses of up to 80 % in tomato production in field. Synthetic fungicides are the most widely used for its controbl, but have harmful consequences. The objective of this work was to test in vitro the antifungal potential of Thevetia peruviana seed extracts against two A. solani isolates. Aqueous, methanol, ethyl acetate and acetone extracts, at concentrations 12.5, 25, 50 and 100 µL/mL were used. Two synthetic fungicides Maneb (5.33 µg/mL) and Dimethomorph + Clorothalonil (3.75 µg/mL) and control (0 µL/mL) were also tested on two A. solani isolates (Mbal and Foum). The investigation was repeated three times. Phytochemical screening, mycelial growth, spore germination and minimum inhibitory concentrations (MIC50 and MIC90) were determined. The results showed that T. peruviana extracts are rich in many families of bioactive compounds such as alkaloids, phenolic compounds and sugars. All extracts tested show high inhibition of mycelial growth (100%) and spore germination (100%) of the two strains at highest concentration (100 µL/mL). Acetone extract at a concentration of 50 µL/mL inhibited mycelial growth by 88.45 and 86.55% and spore germination by 88.33 and 80.33%, respectively for the Mbal and Foum isolates. The lowest MIC50 (16.63 µL/mL) and MIC90 (54.6 µL/mL) were obtained with the acetone extract on the Mbal isolate while the highest MIC50 (27.5 µL/mL) and MIC90 (61.7 µL/mL) were observed with ethyl acetate on the Foum isolate These extracts can therefore be used in the biological control against Alternariose in tomato.","PeriodicalId":510775,"journal":{"name":"Journal of Advances in Microbiology","volume":"11 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141659304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bio-efficacy of Alcoholic Extracts of Botanicals to Mitigate Pea Root Rot Caused by Fusarium solani f.sp. pisi 植物酒精提取物对减轻 Fusarium solani f.sp. pisi 引起的豌豆根腐病的生物功效

Journal of Advances in Microbiology Pub Date : 2024-03-30 DOI: 10.9734/jamb/2024/v24i3805

Khushwinder Kaur, BR. Thakur, Kulveer Singh Dhillon

引用次数: 0

Influence of New Generation Herbicide on Microbial Biomass Dynamics in Sandy Loam Soil 新一代除草剂对沙壤土微生物生物量动态的影响

Journal of Advances in Microbiology Pub Date : 2024-03-30 DOI: 10.9734/jamb/2024/v24i3806

S. K. Patel, Ramjeet Yadav, Balkrishna Namdeo, Nilanjan Chattopadhyaya, Pankaj Kumar

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Morphological, Cultural Characteristics of Post-Harvest Diseases in Onion and its Management through Bio-Agents 洋葱收获后病害的形态、文化特征及其生物制剂管理

Journal of Advances in Microbiology Pub Date : 2024-03-23 DOI: 10.9734/jamb/2024/v24i3804

J. Harish, Namburi Karunakar Reddy, Karan R, Lakshmeesha R, N. Vamsidharreddy, Vineeth M, Divyashree, Chethan P

引用次数: 0

Antibiotics Susceptibility Profile of Non-Typhoidal Salmonella Species Using Selected 3rd Generation Cephalosporins Antibiotics Against Humans and Poultry Faeces in South West, Nigeria 尼日利亚西南部非类毒素沙门氏菌对人类和家禽粪便使用精选的第三代头孢菌素类抗生素的敏感性概况

Journal of Advances in Microbiology Pub Date : 2024-03-20 DOI: 10.9734/jamb/2024/v24i3803

J. O. Ipinlaye, A. Oluyege, F. A. Adewunmi

{"title":"Antibiotics Susceptibility Profile of Non-Typhoidal Salmonella Species Using Selected 3rd Generation Cephalosporins Antibiotics Against Humans and Poultry Faeces in South West, Nigeria","authors":"J. O. Ipinlaye, A. Oluyege, F. A. Adewunmi","doi":"10.9734/jamb/2024/v24i3803","DOIUrl":"https://doi.org/10.9734/jamb/2024/v24i3803","url":null,"abstract":"Introduction: Non-typhoidal Salmonella (NTS) isolates are among the most common bacterial pathogens associated with antimicrobial resistance (AMR) where animals are known to be the major reservoir of NTS despite improvements in hygiene and sanitation.\u0000Aim: This study was conducted to investigate the prevalence of non-typhoidal salmonella species from humans and poultry faeces in Ido-Ekiti using selected 3rd generation cephalosporin antibiotics. Study Location and Duration: The study was carried out at Federal Teaching Hospital Ido Ekiti for 3months between July 2023 and September 2023.\u0000Methodology: A total of 300 faecal samples were collected (100 from humans and 200 from poultry) by random sampling and analyzed by enriching on Selenite F broth and cultured on XLD agar using standard method. Faecal samples were identified using morphology, microscopic and biochemical characteristics. Antibiotic susceptibility test determined using disc diffusion method.\u0000Results: The prevalence of NTS in human and poultry sources were 9% and 11.5% respectively. Antibiotic susceptibility test revealed high resistance rates to 3rd generation Cephalosporins antibiotics among the poultry isolates, especially to Cefuroxime, Cefotaxime and ceftriaxone at 86.9%, 65.2% and 82.6% respectively while Ceftazidime at 88.9% only had an high resistance from human samples as compared to the poultry samples.\u0000Conclusion: The study confirmed that poultries in Ido Ekiti are likely major sources of resistant NTS, and this poses a potential hazard to the public, especially those living close to the poultry and those rearing them.","PeriodicalId":510775,"journal":{"name":"Journal of Advances in Microbiology","volume":"42 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140224596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of Rhizosphere Endophytes and Evaluation of Their Impact on Growth of Chia (Salvia hispanica L.) 根瘤菌内生菌的鉴定及其对芝麻（Salvia hispanica L.）生长影响的评估

Journal of Advances in Microbiology Pub Date : 2024-03-13 DOI: 10.9734/jamb/2024/v24i3802

Sumarani D V, Lakshmeesha, R, J. Harish, K. Kumar, Babu C. V.

{"title":"Identification of Rhizosphere Endophytes and Evaluation of Their Impact on Growth of Chia (Salvia hispanica L.)","authors":"Sumarani D V, Lakshmeesha, R, J. Harish, K. Kumar, Babu C. V.","doi":"10.9734/jamb/2024/v24i3802","DOIUrl":"https://doi.org/10.9734/jamb/2024/v24i3802","url":null,"abstract":"Endophytic microorganisms promote root growth, improve nutrient uptake efficiency and produce more yields. By collaborating with endophytic microorganisms, plants experience enhanced growth. This study was focused on endophytes from Chia plants in Bhukkapatna, Tumakur with the objective of molecular characterization of efficient endophytic microorganisms and its impact on growth of Chia. Twelve bacterial isolates were identified, with eight displaying nitrogen fixation, phosphate solubilisation, potassium fixation, production of IAA, GA, siderophore and exopolysaccharide production. Molecular characterization using 16S rRNA revealed their identities as Pseudomonas fluorescens, Bacillus cereus, Stenotrophomonas maltophilia, and Enterobacter cloacae. Among these, Enterobacter cloacae (CEPB8) exhibited highest positive impact on Chia plant growth. In greenhouse conditions, treatments involving combinations of all four demonstrated the highest plant height (35.467cm) and biomass (158.37 grams) at harvest, surpassing the control group. Combined treatments outperformed individual strains, highlighting synergy. The study affirms that endophytic microorganisms significantly enhanced growth in Chia plant.","PeriodicalId":510775,"journal":{"name":"Journal of Advances in Microbiology","volume":"68 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140245728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigation of Antimicrobial Activity of Aloe vera and it’s Application 芦荟抗菌活性及其应用研究

Journal of Advances in Microbiology Pub Date : 2024-02-29 DOI: 10.9734/jamb/2024/v24i2800

P. Joyruth, Sukirtha, P. Kalaimathy

引用次数: 0