Pharmacognosy Magazine最新文献

{"title":"Resveratrol Suppresses “Metabolic Memory” \u2028by Inhibiting Inflammation and Apoptosis \u2028Through the ROS/TXNIP/NLRP3 Signaling Pathway","authors":"Tingting Jiang, Junxiang Gu, Qing Chang","doi":"10.1177/09731296241229242","DOIUrl":"https://doi.org/10.1177/09731296241229242","url":null,"abstract":"Aim: This research endeavored to explore the impact and underlying mechanisms of resveratrol on the phenomenon of “metabolic memory” in cultured human retinal vascular endothelial cells (HRVECs) under high-glucose (HG) conditions. Materials and Methods: According to the glucose level and treatment, cultured HRVECs were divided into seven groups: normal glucose (NG), HG, high glucose followed by NG (HN), mannitol (Man), resveratrol, thioredoxin-interacting protein (TXNIP)-small interfering ribonucleic acid (siRNA), and N-acetylcysteine (NAC). The expression levels of TXNIP, nucleotide oligomerization domain (NOD)-like receptor family pyrin domain-containing 3 (NLRP3) inflammasome, intercellular adhesion molecule 1 (ICAM-1), caspase-1, interleukin-1β (IL-1β), B-cell lymphoma 2 (Bcl-2), caspase-3, and Bcl-2-associated X (BAX), as well as reactive oxygen species (ROS) production, were measured. Cell apoptosis was assessed through a terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) assay. Results: In HRVECs from the HG group, expression levels of TXNIP, NLRP3, caspase-1, ICAM-1, and IL-1β were upregulated. However, in the HN group, the above upregulations were not reversed. After the administration of resveratrol, the expression levels of TXNIP, NLRP3, and other inflammatory cytokines were significantly reduced. Resveratrol mitigated the elevated ROS production induced by HG conditions. In the NAC group, the expression of TXNIP and inflammatory cytokines was downregulated. TXNIP-siRNA treatment showed similar effects. Resveratrol inhibited apoptosis as well as reversed the downregulation of BCL-2 and the upregulation of caspase-3 and BAX induced by HG conditions. Conclusion: Resveratrol mitigated the HG-induced phenomenon of “metabolic memory” by inhibiting inflammation and apoptosis via modulation of the ROS/TXNIP/NLRP3 signaling pathway in cultured HRVECs. Therefore, resveratrol may have therapeutic potential to treat diabetic retinopathy and related metabolic memory complications.","PeriodicalId":508089,"journal":{"name":"Pharmacognosy Magazine","volume":" 31","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140210485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Research Progress in Regulatory Mechanism \u2028of Valproic Acid on Inflammatory Responses to Burns and Trauma","authors":"Meidi Zhu, Shu-Ming Wang, Rui Liu","doi":"10.1177/09731296241234976","DOIUrl":"https://doi.org/10.1177/09731296241234976","url":null,"abstract":"Inflammation plays an important role in the defense of the human body. The inflammatory response can suppress the spread of pathogens, accelerate the removal of necrotic cells, and repair the damaged tissues and organs. However, an excessive inflammatory response can be fatal as it may lead to cellular apoptosis, necrosis of tissues and organs, and systemic dysfunction. An inflammatory response occurs when the skin is burned or injured, causing more severe and extensive pathological damage to the injured area. For example, inflammation can damage the coagulation system in the capillaries, resulting in the formation of small blood clots in the affected areas. In severe cases, inflammation can lead to necrosis of the injured area and may even affect other uninjured areas, causing hypoxia and inadequate blood supply. The role of valproic acid (VPA), a histone deacetylase inhibitor, has been increasingly recognized in recent years. Evidence suggests that VPA can effectively alleviate tissue and organ injuries secondary to ischemia and hypoxia, and improve the body’s tolerance to ischemia-hypoxia and inflammatory insults, thus increasing the survival rates of the patients. This article reviews the latest research progress made in the mechanisms by which VPA regulates inflammatory response to burns.","PeriodicalId":508089,"journal":{"name":"Pharmacognosy Magazine","volume":" 58","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140210617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cannabis sativa L. Fixed Oil and Its Nanoemulsion: Effect on Diabetes and Dyslipidemia Induced in Rats","authors":"H. Carvalho, Alicia de Melo Santos, Abrahão Victor Tavares de Lima Teixeira dos Santos, Danna Emanuelle Santos Gonçalves, Karyny Roberta Tavares Picanço, Belmira Silva Faria e Souza, José Carlos Tavares Carvalho","doi":"10.1177/09731296241234123","DOIUrl":"https://doi.org/10.1177/09731296241234123","url":null,"abstract":"Background: Diabetes Mellitus (DM) is a syndrome that interferes with energy metabolism and is caused by a decrease and dysfunction of insulin, leading to chronic hyperglycemia. DM associated with dyslipidemia is a severe health risk, increasing the chance of cardiovascular events, such as acute myocardial infarction and stroke. The Cannabis sativa L. fixed oil (CSO) is composed of unsaturated fatty acids and can be crucial in treating metabolic alterations. In addition, the nanoemulsion of C. sativa oil (NCS) has advantages in optimizing treatments. Objectives: This study aimed to evaluate the effects of treatments with CSO and its nanoemulsion (NCS) on induced diabetes and dyslipidemia in Wistar rats. Materials and methods: CSO’s physical-chemical and chromatographic characterization was performed, followed by the preparation of an NE containing 6% CSO. DM was induced in Wistar rats by intraperitoneal injection of streptozotocin (STZ) at a 55 mg/kg dose. Four days later, animals with blood glucose levels exceeding 300 mg/dL were considered diabetic. The rats were then divided into five groups ( n = 5) and treated orally. The groups included a normoglycemic control group (NOR), a diabetic control group, a group treated with metformin (100 mg/kg), a group treated with CSO (400 mg/kg), and a group treated with nanoemulsion (NCS 200 mg/kg). Subsequently, the pancreas’s clinical, biochemical, and histopathological parameters were evaluated. Results: In the chemical profile of CSO, it was observed the majority composition of palmitoleic (14.58%), oleic (12.50%), linoleic (42.40%), and linolenic (8.55%) acids. The results demonstrated that the induction of DM by STZ could reproduce the typical symptoms and clinical signs of DM. It was observed that treatments with CSO and NCS showed a significant improvement ( p < .001) in polydipsia, polyuria, and loss of body mass, as well as a significant reduction ( p < .001) of glucose levels in urine and blood and serum lipids. Histopathology of the pancreas revealed that treatment with CSO and NCS showed an increase in the number of cells in the islets of Langerhans and a decrease in regions devoid of cells, indicating possible cell regeneration. Moreover, insulin levels were significantly increased ( p < .05) in the preferred groups. With dyslipidemia induced by Triton (Tyloxapol), it was observed that the treatment with CSO and NCS significantly decreased the levels of triglycerides ( p < .05) and cholesterol ( p < .001), as well as low-density lipoproteins (LDL) ( p < .01). Conclusion: Treatment with CSO and NCS under the conditions of this study demonstrated an anti-diabetic effect and the ability to act in the reduction of triglyceride, cholesterol, and LDL levels. In this respect, treatments with CSO and NCS act in the control of DM, as well as in the prevention of cardiovascular diseases.","PeriodicalId":508089,"journal":{"name":"Pharmacognosy Magazine","volume":" 19","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140210719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diterpenoids from Scutellaria barbata D. Don Exert the Antiproliferative Effect on Colorectal Cancer Cells Via Hedgehog Signaling Pathway","authors":"Wenwen Wang, Zhongyi Tao, Linglan Tu, Wenkang Huang, Yiping Ye, Lijuan Gao, Xiaoyu Li","doi":"10.1177/09731296241234714","DOIUrl":"https://doi.org/10.1177/09731296241234714","url":null,"abstract":"Background: Scutellaria barbata D. Don, also known as “Banzhilian” in China, the diterpenes of Banzhilian (Bzl-D) is a commonly used herbal medicine for treating various tumors including colorectal cancer (CRC). Objectives: This study aimed to examine the function of Bzl-D in inhibiting CRC development and explore its potential targets and mechanisms involving the Hedgehog (HH) pathway. Materials and methods: To determine the major components in Bzl-D, we performed ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-qTOF-MS) analysis. The proliferation, migration, invasion, and apoptosis abilities of the CRC HT-29 cell line were examined, and the related protein expression was tested. Tumorigenesis after treatment was detected by in vivo experiments. Results: Nineteen diterpenoids were detected, Bzl-D significantly inhibited the cell proliferation and metastasis and induced the apoptosis of HT-29 cells through binding to Sonic HH (SHH) and down-regulating the key proteins (SHH, patched, smoothened, and glioma-associated oncogene) of the HH pathway, thus. Of note, Bzl-D remarkably suppressed the growth of HT-29 xenografts in nude mice. Conclusion: This research demonstrates that Bzl-D is a promising anti-rectal cancer drug, providing a new entry point for targeted therapy of CRC.","PeriodicalId":508089,"journal":{"name":"Pharmacognosy Magazine","volume":"22 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140235411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Review of Traditional Chinese Medicine Syndrome Research on Type 2 Diabetes Mellitus and Its Common Chronic Complications","authors":"Zhiyuan You, Jun Lu, Yifeng Xu, Yiqin Wang, Yiming Hao","doi":"10.1177/09731296241234717","DOIUrl":"https://doi.org/10.1177/09731296241234717","url":null,"abstract":"Type 2 diabetes mellitus is the leading cause of diabetes complications in China, threatening people’s health and quality of life seriously. Over the past few years, with the change in lifestyle and dietary habits, the course of diabetes mellitus is gradually prolonged, and complications in diabetic patients are becoming increasingly common, so scholars have studied the syndrome associated with this disease, which has led to certain research findings. The purpose of this article is to summarise the research progress of diabetes mellitus, diabetic nephropathy, diabetic retinopathy, diabetes mellitus with heart disease, diabetic peripheral neuropathy, and other chronic complications of diabetes mellitus from two aspects of literature and clinical research, present directions and strategies for standardising syndrome types of diabetes mellitus and its common chronic complications.","PeriodicalId":508089,"journal":{"name":"Pharmacognosy Magazine","volume":"6 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140235552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Analgesic Mechanism of Radix Wikstroemia indica “Sweat Soaking Method” Processed Product Based on Metabolomics and Network Pharmacology","authors":"Caiyao Han, Guo Feng, Chenchen Ren, Wei Li, Wen Liu, Gang Liu, Xueli Song, Ju Zhang, Yan Lei, Zhengyan He, Tingting Liu, Kexin Ma, Jinxin Hou","doi":"10.1177/09731296241235450","DOIUrl":"https://doi.org/10.1177/09731296241235450","url":null,"abstract":"Background: RWI has an analgesic effect and is related to metabolites such as 4-pyridoxic acid, l-glutamic acid, and agmatine. It is involved in arginine and proline metabolism, arginine biosynthesis, and alanine, aspartate, and glutamate metabolism. In network pharmacology, there were 404 common targets between RWI and pain diseases, and eight core targets were screened, including SRC, STAT3, and HSP90AA1. GO functional enrichment analysis found that RWI had effects on molecular processes such as protein phosphorylation and response to xenobiotic stimulus, cell composition such as receptor complex and membrane raft, and molecular functions such as enzyme binding. KEGG pathway enrichment analysis obtained 193 pathways. Arginine proline metabolism and nitrogen metabolism are involved in the same pathway as metabolomic analysis. Purpose: To explore the analgesic effect and therapeutic mechanism of RWI processed by “Sweat soaking method”. Materials and methods: The torsion experiment was carried out with acetic acid. The metabolomic analysis of serum samples was carried out based on 1H-NMR technology, and the intersection targets of RWI and pain diseases were screened by network pharmacology for gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. Results: RWI has an analgesic effect and is related to metabolites such as 4-pyridoxic acid, l-glutamic acid, and agmatine. It is involved in arginine and proline metabolism, arginine biosynthesis, and alanine, aspartate, and glutamate metabolism. In network pharmacology, there were 404 common targets between RWI and pain diseases, and eight core targets were screened, including SRC, STAT3, and HSP90AA1. GO functional enrichment analysis found that RWI had effects on molecular processes such as protein phosphorylation and response to xenobiotic stimulus, cell composition such as receptor complex and membrane raft, and molecular functions such as enzyme binding. KEGG pathway enrichment analysis obtained 193 pathways. Arginine proline metabolism and nitrogen metabolism are involved in the same pathway as metabolomic analysis. Conclusion: RWI has an analgesic effect, and its therapeutic mechanism mainly involves arginine and proline metabolism.","PeriodicalId":508089,"journal":{"name":"Pharmacognosy Magazine","volume":"10 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140235046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Applications and Mechanisms of Taohong \u2028Siwu Decoction in the Treatment of \u2028Ischemic Stroke","authors":"Runjia Liu, Yanqing Li, Xiaochen Zhang, Kaixuan Tang, Jiatong Li, Tianhe Fang, Xuanhe Tian, Bingbing Han","doi":"10.1177/09731296241235435","DOIUrl":"https://doi.org/10.1177/09731296241235435","url":null,"abstract":"Ischemic stroke is a kind of sudden vascular disease of the brain brought on by ischemic hypoxic necrosis of the brain tissue which is becoming a significant global source of death and long-term impairment. Present common treatment options for cerebral ischemia including thrombolytic therapy, mechanical embolization, and intracranial arterioplasty can only benefit a few patients due to the narrow therapeutic time windows. Traditional Chinese Medicine (TCM) prescriptions possess the qualities of multi-ingredients, multi-targets, synergistic and multi-efficacy advantages as a means of treating ischemic stroke. Taohong Siwu Decoction (THSWD) is a famous TCM prescription that promotes blood flow, removes blood clots, adds nourishment, and tonifies blood. Clinical and laboratory research results show the significant advantages of THSWD in ischemic stroke treatment. This review summarizes THSWD’s natural materials and processing methods, pharmacological effects, and related mechanisms to treat ischemic stroke. Its mechanism of action focuses on improving hemorheology, anti-inflammatory response, reducing excitotoxicity, anti-calcium overload, anti-oxidative stress, etc. Moreover, we systematically summarize THSWD and its use in combination with other treatments. Finally, the research obstacles of THSWD in the treatment of ischemic stroke are summarized, and the application prospects in the medical field are prospected.","PeriodicalId":508089,"journal":{"name":"Pharmacognosy Magazine","volume":"41 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140238748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Picroside Ⅱ Inhibits Glycolysis and Migration in HepG2 Cells","authors":"Xia Lei, Si-Cheng Yang, Kang-Bo Peng, Meng-Fan Wang, He-Zhen Wu, Yan-Fang Yang","doi":"10.1177/09731296231220980","DOIUrl":"https://doi.org/10.1177/09731296231220980","url":null,"abstract":"Objectives: The objective of this study was to investigate the impacts and mechanisms of Picroside II on hepatocellular carcinoma (HCC). Materials and Methods: The GeneCards database was mined for HCC-related targets. The targets of Picroside Ⅱ were predicted using PharmMapper and SwissTarget Prediction. The molecular complex detection (MCODE) plugin in Cytoscape was used to screen core genes. The DAVID database was subsequently used to enrich the core targets by Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG), which was used to determine the possible mode of action of Picroside II on HCC. Gene Expression Profiling Interactive Analysis 2 (GEPIA2) examined core targets and patient mortality. Auto Dock 4.2.6 was used to simulate the docking of potential targets with Picroside II. Then, surface plasmon resonance (SPR) was used to validate the targets’ docking ability. Finally, Western blotting and in vitro experiments were used to evaluate the effect of Picroside II on HepG2 cells. Results: The result of network pharmacology showed that 94 genes may be possible targets for Picroside II treating of HCC, with GPI, LGALS3, SRC, HRAS, HSP90AA1, MMP9, PPARG, SERPINE1, and VEGFA being the most promising. GPI and Picroside II have strong virtual docking capabilities, and SPR tests verified their binding ability. In vitro studies revealed that Picroside II could suppress HepG2 cell migration. It has been demonstrated that Picroside II can stop tumors from migrating by attaching to the glycolytic proteins GPI and suppressing the downstream glycolytic pathway proteins ALDOA and GAPDH. Picroside II’s potential new target for treating tumors is GPI. Conclusion: The results of this study suggest that Picroside Ⅱ can affect glucose metabolism by binding to glycolytic protein GPI and that this protein is a potential new target for Picroside Ⅱ in the treatment of tumors.","PeriodicalId":508089,"journal":{"name":"Pharmacognosy Magazine","volume":"124 23","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140237842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic Alleviation and Mechanism of Glabridin Liposome on Histamine-induced Atopic Dermatitis","authors":"Yongjie Lu, Lushi Cheng, Lu Ren, Dongqiu Chen, Shumin Guan, Siyang Zhu, Xiangye Xu, Bing Zhang, Minghui Tang, Chijian Zhang, Yong Ai, Lanyue Zhang, Tinggang He","doi":"10.1177/09731296231225512","DOIUrl":"https://doi.org/10.1177/09731296231225512","url":null,"abstract":"Background: One of the primary flavonoids found in the Glycyrrhiza glabra is called glabridin, which has anti-inflammatory, anti-bacterial, and antineoplastic effects. However, the insolubility of glabridin in water limits its application. Liposomes can increase the solubility of insoluble drugs and improve their bioavailability. Objective: We examined the potential for the treatment of glabridin liposomes on histamine-induced atopic dermatitis. Materials and Methods: After GL treatment, histopathology, inflammatory cytokines, and atopic dermatitis-related proteins were used to evaluate the therapeutic effect of GL. Results: Glabridin liposomes alleviated histamine-induced scratching behavior; reduced mast cell proliferation, infiltration, and degranulation; and restrained the expression of associated pro-inflammatory cytokines. Additionally, glabridin liposomes restored nerve growth factor to normal levels and enhanced the expression of filaggrin to promote cuticle growth and repair skin damage caused by atopic dermatitis. Conclusion: Glabridin liposomes may relieve histamine-induced skin hypersensitivity and cortical hyperplasia by inhibiting the production of inflammatory cytokines, demonstrating their potential for the clinical treatment of atopic dermatitis.","PeriodicalId":508089,"journal":{"name":"Pharmacognosy Magazine","volume":"23 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140252552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Induction and Characterization of Tetraploids from Astragalus membranaceus (Fisch.) Bge. Seeds","authors":"Shu-ling Zhang, Xin Zhang, Meng Wang, Li Dong, Gui-xia Liu","doi":"10.1177/09731296231198574","DOIUrl":"https://doi.org/10.1177/09731296231198574","url":null,"abstract":"Astragalus membranaceus (Fisch.) Bge., as a Traditional Chinese medicine, not only has ornamental and medicinal value but also plays a role in the effect of sand fixation. At present, the resources of wild A. membranaceus are exhausted with the deterioration of the ecological environment and the increase in market demand. Moreover, there are no excellent varieties for the artificial cultivation of A. membranaceus to meet the market demand. To establish a method to create an efficient induction of tetraploids by colchicine technique in A. membranaceus. The germinating seeds were soaked in different concentrations of colchicine solution and exposure times to induce tetraploids in A. membranaceus, and the induced plants were identified by morphological characteristics comparison and root-tip chromosome determination. The contents of polysaccharides, saponins, and flavonoids and the activity of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) were measured in diploid and tetraploid plants. The results showed that the highest tetraploid induction ratio of germinating seeds in A. membranaceus is obtained from the 0.2% (w/v) colchicine treatment for 36 h, and the chromosome number of tetraploid seedlings was 2n = 4x = 32. The morphological traits and anatomical structure characteristics of leaves from tetraploid plants exhibited significant differences from the diploid plants. The contents of polysaccharides, saponins, and flavonoids and the activity of SOD, POD, and CAT are also increased in tetraploid plants. In summary, our study provided an effective method to induce A. membranaceus polyploidy, which could be used to develop new germplasm innovations for improving the medicinal value of A. membranaceus.","PeriodicalId":508089,"journal":{"name":"Pharmacognosy Magazine","volume":"34 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140261831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}