Anales De La Real Academia Nacional De Farmacia最新文献_第10页

The Origins of Enantioselective Organocatalysis and the 2021 Nobel Prize in Chemistry 对映选择性有机催化的起源和2021年诺贝尔化学奖

Anales De La Real Academia Nacional De Farmacia Pub Date : 2021-01-01 DOI: 10.53519/analesranf.2021.87.04.09

José Carlos Menéndez Ramos

引用次数: 0

RELATIONSHIP OF THE VIROLOGIST PROFESSOR ADOLFO GARCÍA SASTRE WITH THE UNIVERSITY OF SALAMANCA AND THE ROYAL NATIONAL ACADEMY OFPHARMACY 病毒学教授adolfo garcÍa sastre与萨拉曼卡大学和皇家国家药学院的关系

Anales De La Real Academia Nacional De Farmacia Pub Date : 2021-01-01 DOI: 10.53519/analesranf.2021.87.01.001

José Antonio Cabezas Fernández del Campo, Mariano Esteban Rodríguez

{"title":"RELATIONSHIP OF THE VIROLOGIST PROFESSOR ADOLFO GARCÍA SASTRE WITH THE UNIVERSITY OF SALAMANCA AND THE ROYAL NATIONAL ACADEMY OF\u0000PHARMACY","authors":"José Antonio Cabezas Fernández del Campo, Mariano Esteban Rodríguez","doi":"10.53519/analesranf.2021.87.01.001","DOIUrl":"https://doi.org/10.53519/analesranf.2021.87.01.001","url":null,"abstract":"We give some biographical details of the virologist Professor Adolfo Garcia Sastre, as a Graduate student (1981-1986) in the Biology School of University of Salamanca and during his PhD Thesis (1986-1990) in the Department of Biochemistry and Molecular Biology (Chairman Prof J.A. Cabezas), under the supervision of Prof. Enrique Villlar and obtaining the highest academic marks. The research lines that he established in collaboration with his Thesis director, with Prof. J.A Cabezas and others, as well as his results during his stay at the Pasteur Institute in Paris, are also highlighted. His findings in this period were published in prestigious Virology and Biochemistry journals and presented at national and international meetings. Thereafter, when he moved to Mount Sinai in New York, he met Prof Mariano Esteban, then working at Downstate Medical Center in New York, SUNY, and both, in collaboration with the group of Prof. Ruth Nussenzweig and Fidel Zavala at New York University, set up seminal immunological studies that are the basis for combined vaccination approaches, prime/boost and activation of CD8+ T cells, now widely used in preclinical and clinical studies. The scientific research contributions of Prof. García Sastre are growing at an exponential rate, opening new horizons in understanding the molecular biology of emerging viruses, their pathology, virus-host cell interactions and strategies of virus control.","PeriodicalId":50795,"journal":{"name":"Anales De La Real Academia Nacional De Farmacia","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71085564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phytopatogenic fungi in terrestrial and aquatic environments present in Latin America 拉丁美洲陆地和水生环境中的植生真菌

Anales De La Real Academia Nacional De Farmacia Pub Date : 2021-01-01 DOI: 10.53519/analesranf.2021.87.04.01

Astrid Maribel Aguilera Becerra, Jeymi Patricia Higuera Blanco, Eileen Oriana Univio Munevar

{"title":"Phytopatogenic fungi in terrestrial and aquatic environments present in Latin America","authors":"Astrid Maribel Aguilera Becerra, Jeymi Patricia Higuera Blanco, Eileen Oriana Univio Munevar","doi":"10.53519/analesranf.2021.87.04.01","DOIUrl":"https://doi.org/10.53519/analesranf.2021.87.04.01","url":null,"abstract":"Objective: The purpose of this literature review is to know the different phytopathogenic fungi present in these two types of environments, and the factors involved in their presence or absence. Materials and methods: For the construction of this article, a bibliographic search was carried out in the following databases: Pubmed, Science direct, Google scholar, Scielo and NCBI in English and Spanish, using the following descriptors: Fungi, Aquatic microorganisms, and Fungi in soil. Results: In this bibliographic review, the presence of Aspergillus sp, Fusarium sp, Mucor sp, Penicillium sp, Alternaria sp, Trichoderma sp, Blastomyces sp, Geotrichum sp, etc. was found in most of the aquatic environments. In addition, in terrestrial environments, fungi of the genus Rhizopus sp, Fusarium sp, Beauveria sp, Absidia sp, Mucor sp, Aspergillus sp, Penicillium sp, Paecilomyces sp, etc. were found. Likewise, we investigated the factors that determine the concentration and diversity in aquatic and terrestrial environments, in all types of fungi. In aquatic environments, these are: temperature, rainfall, water velocity, nutrient status, anthropogenic impact and abundance of decomposing matter. On the other hand, in terrestrial environments, we find: pH, anthropogenic impact, and the presence of pollutants. Conclusion: In the case of fungi in fresh water, the genus Aspergillus sp was the most representative, while in the terrestrial environment the genus Fusarium sp, and Rhizopus sp, were the most predominant with their appearance in most of the studies cited in this review.","PeriodicalId":50795,"journal":{"name":"Anales De La Real Academia Nacional De Farmacia","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71087233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ultra-processed Food Intake and Risk of Cardiovascular Disease: a systematic review 超加工食品摄入与心血管疾病风险:一项系统综述

Anales De La Real Academia Nacional De Farmacia Pub Date : 2021-01-01 DOI: 10.53519/analesranf.2021.87.03.09

A. Martí Del Moral, Sofía Aimi Arrúe Ishiyama

{"title":"Ultra-processed Food Intake and Risk of Cardiovascular Disease: a systematic review","authors":"A. Martí Del Moral, Sofía Aimi Arrúe Ishiyama","doi":"10.53519/analesranf.2021.87.03.09","DOIUrl":"https://doi.org/10.53519/analesranf.2021.87.03.09","url":null,"abstract":"Cardiovascular disease (CVD) is the leading cause of mortality worldwide. An unhealthy diet, among other risk factors, can be involved in the development of the disease. This type of diet is usually characterized by being low in unprocessed foods (fruits, vegetables, etc.) and high in ultra-processed foods (UPFs). It has been suggested that a high consumption of UPFs could be related to the development of CVD. However, as UPFs are a broad group in which multiple foods with different compositions coexist, it is not only difficult but also a generalization to draw a conclusion about their possible association. The present systematic review studies the relationship between the consumption of UPFs and the risk of CVD. A systematic review was performed in the PubMed database. PRISMA criteria were applied to identify articles that met the following criteria: studies performed in humans, in English or Spanish, and be original articles, excluding meta-analyses and reviews. The search was performed using the terms “Ultra-processed food” or “Fast food” and “Cardiovascular disease” or “Cardiovascular risk factor”, with a total of 10 articles included: 4 prospective cohort studies, 4 cross-sectional studies and 2 modeling studies. An association between the consumption of UPFs and CVD was found in all the studies analyzed. However, there are limitations with respect to the use of different food classification systems according to the degree of processing. This systematic review concludes that more studies are needed to establish with greater evidence the relationship between the consumption of UPFs and risk of CVD.","PeriodicalId":50795,"journal":{"name":"Anales De La Real Academia Nacional De Farmacia","volume":"68 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71087413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Crisis of the Colleges of the University of Salamanca (XVIII-XX centuries). Its link with the Diputación (XIX century) 萨拉曼卡大学学院的危机(十八至二十世纪)。它与Diputación的联系(19世纪)

Anales De La Real Academia Nacional De Farmacia Pub Date : 2021-01-01 DOI: 10.53519/analesranf.2021.87.04.05

José Antonio Cabezas Fernández del Campo

引用次数: 0

Biotin interference in several electrochemiluminescence immunoassays (eclia) 生物素在几种电化学发光免疫测定中的干扰

Anales De La Real Academia Nacional De Farmacia Pub Date : 2021-01-01 DOI: 10.53519/analesranf.2021.87.03.02

J. L. Martı́n-Calderón

{"title":"Biotin interference in several electrochemiluminescence immunoassays (eclia)","authors":"J. L. Martı́n-Calderón","doi":"10.53519/analesranf.2021.87.03.02","DOIUrl":"https://doi.org/10.53519/analesranf.2021.87.03.02","url":null,"abstract":"– Background: The aim of this study is to define the interference of biotin in several endocrine, tumor marker, and vitamin assays performed by an electrochemiluminescence method, trying to determinate the critical level that causes biotin interference. – Material and methods: Working biotin solutions were prepared in phosphate-buffered saline (PBS) at different concentrations (10000, 7500, 5000, 2500, 1250, 625, and 312.5 ng/mL), which were spiked on the samples to obtain final concentrations ten-fold lower. Each serum biotin dilution was tested in triplicate, using at least two levels of analytes. Determinations of several endocrine, vitamins, tumor and bone markers were carried-out with eletrochemilumenescent immunoassays on the cobas e801 and cobas e411. Comparison between the results obtained by analyzing the biotin-spiked samples and the reference PBS-spiked samples was performed using Microsoft Excel. The relative bias with the interfering-free specimen was calculated for each biotin concentration. Interference was considered significant when the relative bias exceeded 10%. Glick´s interferographs were performed plotting the percentage of change vs. biotin concentration. – Results: Analyte concentrations were spuriously decreased in 12 sandwich immunoassays and falsely increased in 11 competitive immunoassays. However thyrotropin and CA 15.3 antigen were not significantly affected. – Conclusions: Except CA 15.3 and TSH, the methods tested were susceptible to biotin interference. Falsely low values occurred in sandwich assays and high bias in competitive assays. Clinicians and laboratorians should be aware of the medical importance of biotin interference as a cause of misdiagnosis and incorrect treatment.","PeriodicalId":50795,"journal":{"name":"Anales De La Real Academia Nacional De Farmacia","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71086593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ANCESTRAL RECONSTRUCTION OF A β-LACTAMASE AND COMPARISON WITH ITS EXTANT PROTEINS β-内酰胺酶的祖先重建及其与现有蛋白的比较

Anales De La Real Academia Nacional De Farmacia Pub Date : 2021-01-01 DOI: 10.53519/analesranf.2021.87.02.05

Gonzalo Fernández Balaguer, C. del Águila, Carolina Hurtado Marcos, Rubén Agudo Torres

{"title":"ANCESTRAL RECONSTRUCTION OF A β-LACTAMASE AND COMPARISON WITH ITS EXTANT PROTEINS","authors":"Gonzalo Fernández Balaguer, C. del Águila, Carolina Hurtado Marcos, Rubén Agudo Torres","doi":"10.53519/analesranf.2021.87.02.05","DOIUrl":"https://doi.org/10.53519/analesranf.2021.87.02.05","url":null,"abstract":"The β-lactamases are proteins of bacterial origin that are characterized by hydrolyzing antibiotics β-lactams, conferring microbial resistance against them. They are a heterogeneous family of proteins very relevant from a health point of view due to the ease they present to acquire resistance to new drugs due to their high capacity for evolution. The in vitro evolution of these proteins has served not only to develop their characterization and improve their knowledge, but as a new line of research that allows to predictively identify residues involved in the acquisition of antibiotic resistance. At the same time, the method of ancestral protein reconstruction has been revealed as a novel and useful tool to understand the evolution of β-lactamases and understand some of their characteristics such as their promiscuity. In this work, a study of ancestral β-lactamases reconstructed from the phylogeny of existing class A β-lactamases has been carried out. Of the four ancestral proteins studied, one has been obtained that is functional and has compared its hydrolytic activity with that of four of its current counterparts against eight β-lactam drugs. This ancestral protein has been shown to have a more generalistic antibiotic activity than any of the current proteins studied. In addition, the active ancestral protein showed more resistance to one of the drugs used than the rest of β-lactamases existing. Finally these results have been discussed and from them it is argued why reconstructed ancestral sequences can be a very attractive starting point when it comes to direct evolution of proteins for obtaining proteins of biotechnological interest.","PeriodicalId":50795,"journal":{"name":"Anales De La Real Academia Nacional De Farmacia","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71086482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Protective effects of proanthocyanidins at the intestinal levelagainst thermooxidative stress due to ingestion of sunflower oil used in frying 原花青素在肠道水平上对因食用油炸用葵花籽油而引起的热氧化应激的保护作用

Anales De La Real Academia Nacional De Farmacia Pub Date : 2021-01-01 DOI: 10.53519/analesranf.2021.87.03.01

R. Redondo-Castillejo, A. Bocanegra, Alba Garcimartín, A. Macho-González, M. Hernández-Martín, Sofía I. García-Moreno, Beatriz Raso-Esteban, M. López-Oliva, J. Benedí, S. Bastida

{"title":"Protective effects of proanthocyanidins at the intestinal levelagainst thermooxidative stress due to ingestion of sunflower oil used in frying","authors":"R. Redondo-Castillejo, A. Bocanegra, Alba Garcimartín, A. Macho-González, M. Hernández-Martín, Sofía I. García-Moreno, Beatriz Raso-Esteban, M. López-Oliva, J. Benedí, S. Bastida","doi":"10.53519/analesranf.2021.87.03.01","DOIUrl":"https://doi.org/10.53519/analesranf.2021.87.03.01","url":null,"abstract":"The repeated frying of food in sunflower oil produces thermo-oxidation and polymerization, potentially negative for health. The reaction of the gastrointestinal system to these compounds is unknown. Proanthocyanidins have antioxidant and lipid-lowering properties. We hypothesize that a carob-fruit extract rich in proanthocyanidins (CFE) improves the defense of the intestine against thermo-oxidative aggression. In sunflower oil, the thermo-oxidation produced by repeated frying of fish was studied while, in Wistar rats, the effect of jointly administering altered sunflower oil and CFE on: the digestion of thermo-oxidized compounds, postprandial lipaemia, proteins involved in lipid absorption, antioxidant and hemoxygenase-1 activity and expression in the small intestine. Twelve male Wistar rats, 200-250 g were cannulated for one week with the mixture of the thermo-oxidized oil-CFE or the thermo-oxidized oil. Thermo-oxidation was determined in sunflower oil and in the postprandial-fat of the gastrointestinal lumen, postprandial lipaemia, the proteins NPC1L1, ACAT-2 and MTP, and the antioxidant status in the duodenum, jejunum and ileum. High thermo-oxidation and polymerization levels were found on sunflower oil and fat in the gastrointestinal lumen. The administration of thermo-oxidized sunflower oil-CFE decreases the digestibility of the oil and the polymeric/thermo-oxidized compounds, reduces postprandial lipaemia, increases NPC1L1, ACAT-2, and MTP, and improves the intestinal antioxidant status and excretion of fecal polymers. The use of CFE reduces postprandial lipaemia and guarantees an adequate intestinal antioxidant status against thermo-oxidized lipids.","PeriodicalId":50795,"journal":{"name":"Anales De La Real Academia Nacional De Farmacia","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71086677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Benito Pérez Galdós (1843-1920) and the health of his time 贝尼托·帕萨雷兹Galdós(1843-1920)和他那个时代的健康状况

Anales De La Real Academia Nacional De Farmacia Pub Date : 2021-01-01 DOI: 10.53519/analesranf.2021.87.03.03

F. J. Puerto Sarmiento

引用次数: 0

REGULATION OF THE INTESTINAL INFLAMMATORY RESPONSE THROUGH THE INGESTION OF MAILLARD COMPOUNDS AND THEIR INTERACTION WITH RAGE AND GPR43 RECEPTORS 通过摄入美拉德化合物及其与rage和gpr43受体的相互作用调节肠道炎症反应

Anales De La Real Academia Nacional De Farmacia Pub Date : 2021-01-01 DOI: 10.53519/analesranf.2021.87.02.04

S. Pastoriza, J. Rufián‐Henares

{"title":"REGULATION OF THE INTESTINAL INFLAMMATORY RESPONSE THROUGH THE INGESTION OF MAILLARD COMPOUNDS AND THEIR INTERACTION WITH RAGE AND GPR43 RECEPTORS","authors":"S. Pastoriza, J. Rufián‐Henares","doi":"10.53519/analesranf.2021.87.02.04","DOIUrl":"https://doi.org/10.53519/analesranf.2021.87.02.04","url":null,"abstract":"Molecular damage signals attract neutrophils to sites of infection or inflammation. The G-protein coupled receptor (GPR43) and the receptor for advanced glicosilation compounds (RAGE) recognize short-chain fatty acids (propionate and butyrate) and AGEs (advanced glycosylation compounds) respectively, both receptors being abundantly expressed in neutrophils and intestinal epithelial cells. The functional role that activation of these receptors plays in the in vivo orchestration of the immune response is unclear. Our work examines the effect of the ingestion of AGEs on the immune response, both in healthy mice and in mice that were induced to colitis, using transgenic mice deficient in GPR43 or RAGE receptors. One of the main findings is that both the GPR43 receptor and RAGE are necessary for the recruitment of neutrophils in a model of intestinal inflammation due to mucosal barrier injury. We have also verified that the AGEs ingested with the diet promote the appearance of an imbalance in the inflammatory balance at the intestinal level, giving rise to a pro-inflammatory status. We have also show that carboxymethylisine (CML), a specific type of AGE, is capable of stimulating the GPR43 receptor and acting as a neutrophil chemoattraction factor. Finally, we have tested the treatment with sRAGE, a protein capable of capturing free AGEs. This procedure could be a promising therapy for the treatment of inflammatory bowel disease.","PeriodicalId":50795,"journal":{"name":"Anales De La Real Academia Nacional De Farmacia","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71086549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0