{"title":"MetaWin 3: open-source software for meta-analysis","authors":"Michael S. Rosenberg","doi":"10.3389/fbinf.2024.1305969","DOIUrl":"https://doi.org/10.3389/fbinf.2024.1305969","url":null,"abstract":"The rise of research synthesis and systematic reviews over the last 25 years has been aided by a series of software packages providing simple and accessible GUI interfaces which are intuitively easy to use by novice analysts and users. Development of many of these packages has been abandoned over time due to a variety of factors, leaving a gap in the software infrastructure available for meta-analysis. To fulfill the continued demand for a GUI-based meta-analytic system, we have now released MetaWin 3 as free, open-source, multi-platform software. MetaWin3 is written in Python and developed from scratch relative to earlier versions. The codebase is available on Github, with pre-compiled executables for both Windows and macOS available from the MetaWin website. MetaWin includes standardized effect size calculations, exploratory and publication bias analyses, and allows for both simple and complex explanatory models of variation within a meta-analytic framework, including meta-regression, using traditional least-squares/moments estimation.","PeriodicalId":507586,"journal":{"name":"Frontiers in Bioinformatics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139850872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Choudhury, Nisha Bajiya, Sumeet Patiyal, G. Raghava
{"title":"MRSLpred—a hybrid approach for predicting multi-label subcellular localization of mRNA at the genome scale","authors":"S. Choudhury, Nisha Bajiya, Sumeet Patiyal, G. Raghava","doi":"10.3389/fbinf.2024.1341479","DOIUrl":"https://doi.org/10.3389/fbinf.2024.1341479","url":null,"abstract":"In the past, several methods have been developed for predicting the single-label subcellular localization of messenger RNA (mRNA). However, only limited methods are designed to predict the multi-label subcellular localization of mRNA. Furthermore, the existing methods are slow and cannot be implemented at a transcriptome scale. In this study, a fast and reliable method has been developed for predicting the multi-label subcellular localization of mRNA that can be implemented at a genome scale. Machine learning-based methods have been developed using mRNA sequence composition, where the XGBoost-based classifier achieved an average area under the receiver operator characteristic (AUROC) of 0.709 (0.668–0.732). In addition to alignment-free methods, we developed alignment-based methods using motif search techniques. Finally, a hybrid technique that combines the XGBoost model and the motif-based approach has been developed, achieving an average AUROC of 0.742 (0.708–0.816). Our method—MRSLpred—outperforms the existing state-of-the-art classifier in terms of performance and computation efficiency. A publicly accessible webserver and a standalone tool have been developed to facilitate researchers (webserver: https://webs.iiitd.edu.in/raghava/mrslpred/).","PeriodicalId":507586,"journal":{"name":"Frontiers in Bioinformatics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139800099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Choudhury, Nisha Bajiya, Sumeet Patiyal, G. Raghava
{"title":"MRSLpred—a hybrid approach for predicting multi-label subcellular localization of mRNA at the genome scale","authors":"S. Choudhury, Nisha Bajiya, Sumeet Patiyal, G. Raghava","doi":"10.3389/fbinf.2024.1341479","DOIUrl":"https://doi.org/10.3389/fbinf.2024.1341479","url":null,"abstract":"In the past, several methods have been developed for predicting the single-label subcellular localization of messenger RNA (mRNA). However, only limited methods are designed to predict the multi-label subcellular localization of mRNA. Furthermore, the existing methods are slow and cannot be implemented at a transcriptome scale. In this study, a fast and reliable method has been developed for predicting the multi-label subcellular localization of mRNA that can be implemented at a genome scale. Machine learning-based methods have been developed using mRNA sequence composition, where the XGBoost-based classifier achieved an average area under the receiver operator characteristic (AUROC) of 0.709 (0.668–0.732). In addition to alignment-free methods, we developed alignment-based methods using motif search techniques. Finally, a hybrid technique that combines the XGBoost model and the motif-based approach has been developed, achieving an average AUROC of 0.742 (0.708–0.816). Our method—MRSLpred—outperforms the existing state-of-the-art classifier in terms of performance and computation efficiency. A publicly accessible webserver and a standalone tool have been developed to facilitate researchers (webserver: https://webs.iiitd.edu.in/raghava/mrslpred/).","PeriodicalId":507586,"journal":{"name":"Frontiers in Bioinformatics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139859985","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jannes Peeters, Daniël M. Bot, Gustavo Rovelo Ruiz, Jan Aerts
{"title":"Snowflake: visualizing microbiome abundance tables as multivariate bipartite graphs","authors":"Jannes Peeters, Daniël M. Bot, Gustavo Rovelo Ruiz, Jan Aerts","doi":"10.3389/fbinf.2024.1331043","DOIUrl":"https://doi.org/10.3389/fbinf.2024.1331043","url":null,"abstract":"Current visualizations in microbiome research rely on aggregations in taxonomic classifications or do not show less abundant taxa. We introduce Snowflake: a new visualization method that creates a clear overview of the microbiome composition in collected samples without losing any information due to classification or neglecting less abundant reads. Snowflake displays every observed OTU/ASV in the microbiome abundance table and provides a solution to include the data’s hierarchical structure and additional information obtained from downstream analysis (e.g., alpha- and beta-diversity) and metadata. Based on the value-driven ICE-T evaluation methodology, Snowflake was positively received. Experts in microbiome research found the visualizations to be user-friendly and detailed and liked the possibility of including and relating additional information to the microbiome’s composition. Exploring the topological structure of the microbiome abundance table allows them to quickly identify which taxa are unique to specific samples and which are shared among multiple samples (i.e., separating sample-specific taxa from the core microbiome), and see the compositional differences between samples. An R package for constructing and visualizing Snowflake microbiome composition graphs is available at https://gitlab.com/vda-lab/snowflake.","PeriodicalId":507586,"journal":{"name":"Frontiers in Bioinformatics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139865363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jannes Peeters, Daniël M. Bot, Gustavo Rovelo Ruiz, Jan Aerts
{"title":"Snowflake: visualizing microbiome abundance tables as multivariate bipartite graphs","authors":"Jannes Peeters, Daniël M. Bot, Gustavo Rovelo Ruiz, Jan Aerts","doi":"10.3389/fbinf.2024.1331043","DOIUrl":"https://doi.org/10.3389/fbinf.2024.1331043","url":null,"abstract":"Current visualizations in microbiome research rely on aggregations in taxonomic classifications or do not show less abundant taxa. We introduce Snowflake: a new visualization method that creates a clear overview of the microbiome composition in collected samples without losing any information due to classification or neglecting less abundant reads. Snowflake displays every observed OTU/ASV in the microbiome abundance table and provides a solution to include the data’s hierarchical structure and additional information obtained from downstream analysis (e.g., alpha- and beta-diversity) and metadata. Based on the value-driven ICE-T evaluation methodology, Snowflake was positively received. Experts in microbiome research found the visualizations to be user-friendly and detailed and liked the possibility of including and relating additional information to the microbiome’s composition. Exploring the topological structure of the microbiome abundance table allows them to quickly identify which taxa are unique to specific samples and which are shared among multiple samples (i.e., separating sample-specific taxa from the core microbiome), and see the compositional differences between samples. An R package for constructing and visualizing Snowflake microbiome composition graphs is available at https://gitlab.com/vda-lab/snowflake.","PeriodicalId":507586,"journal":{"name":"Frontiers in Bioinformatics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139805642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}