{"title":"In Silico Comparative Analysis of Different vacA Genes of Helicobacter pylori","authors":"H. Atanda, E. K. Oladipo, S. E. Olufemi","doi":"10.18061/ojs.v123i2.9135","DOIUrl":"https://doi.org/10.18061/ojs.v123i2.9135","url":null,"abstract":"Helicobacter pylori is a class I carcinogen responsible for 90% of gastrointestinal and gastroduodenaldisorders, including gastric cancer and peptic ulcer disease. The virulence and pathogenicity peculiar to H. pylorihave been associated with several genes, including cytotoxin associated gene (cagA), vacuolating cytotoxin A(vacA), outer inflammatory protein A (oipA), and duodenal ulcer promoting (dupA). This study explored therelationship between African-generated vacA genes with genes from other regions with high gastrointestinaldisorder prevalence. Nucleotide sequences of 228 vacA genes of H. pylori were retrieved from the NationalCentre for Biotechnology Information (NCBI). Pairwise and multiple sequence alignment was carried out on228 vacA nucleotide sequences using MEGA 10.2.4 software to identify regions of similarities. Phylogeneticanalysis, also using MEGA software, was carried out to establish the evolutionary relationships between allextracted sequences. Analysis for conserved domain was also performed on the NCBI Conserved DomainDatabase to better understand each geographical data's properties. After the evolutionary analysis, it wasobserved that South African vacA genes were more closely related to genes from Mexico, Italy, Spain, andGermany—with Italy having the highest occurring relationship. Conserved domain analysis showed 2 highlyconserved superfamilies, cl20029 and cl22877, and 2 protein family models, pfam02691 and pfam03797.The results demonstrate relatedness of vacA genes from the African region to the European region; Italy,Mexico, and Spain. The study shows the biogeographical diversity among vacA genes and emphasizes thedegree of domain conservation across each gene. It also shows the need for a holistic assessment of thevirulent genes in H. pylori.","PeriodicalId":507306,"journal":{"name":"The Ohio Journal of Science","volume":"17 s1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141687904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"First Documented Nesting of American White Pelicans (Pelecanus erythrorhynchos) in Ohio, USA","authors":"Bruce N. Buckingham, Laura J. Kearns","doi":"10.18061/ojs.v123i2.9541","DOIUrl":"https://doi.org/10.18061/ojs.v123i2.9541","url":null,"abstract":"The discovery and documentation of a new breeding species in a defined area, such as a state, is a crucial first step in understanding the basic natural history of a species and its consequent needs for management and conservation. The American White Pelican has gradually expanded its breeding range from the prairies of North America into the Great Lakes region. While conducting a census on Herring Gulls (Larus argentatus) at least 4 nests of pelicans with either eggs or young were found. Further census showed a minimum of 12 almost fledged young. This report documents the first confirmed nesting of the American White Pelican in Ohio. This first nesting was observed in May 2023 on Turning Point Island, an artificial island in Sandusky Bay, Erie County, Ohio, in the western basin of Lake Erie. Continued nesting of pelicans in Ohio is expected in future years at this location and other suitable sites in the area. This species is likely to need future monitoring and management.","PeriodicalId":507306,"journal":{"name":"The Ohio Journal of Science","volume":"106 44","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140379760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Abstracts from the 2022 OAS Annual Meeting","authors":"The Ohio Academy of Science","doi":"10.18061/ojs.v122i1.9728","DOIUrl":"https://doi.org/10.18061/ojs.v122i1.9728","url":null,"abstract":"No abstract available.","PeriodicalId":507306,"journal":{"name":"The Ohio Journal of Science","volume":"55 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139179574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"An Assessment of In-stream Sampling Activity of Macroinvertebrates and Stream Channelization in Dug Run, Allen County, Ohio, USA","authors":"D. Zuwerink, Zakiah Le’Flore, Seth Lochtefeld","doi":"10.18061/ojs.v123i2.9205","DOIUrl":"https://doi.org/10.18061/ojs.v123i2.9205","url":null,"abstract":"Macroinvertebrates are good indicators of stream quality. Changes in populations of sensitive macroinvertebrates help to show stressors to the stream. Student sampling of a section of Dug Run in northwestern Ohio has occurred since 2015. This work has been to identify how changes on the campus including construction, tree removal, and channelization may be impacting stream macroinvertebrates. Student sampling, however, also causes disturbances that may negatively impact macroinvertebrate populations. A break in student sampling—due first to the use of an adjacent off-site location in 2019 and then to COVID-19 beginning in 2020—was expected to impact the number of mayfly nymphs and caddisfly larvae captured, both considered sensitive macroinvertebrates in the stream. To measure the impact of channelization in Dug Run, the study area was split into a channelized reach, an upstream reach, and a downstream reach. Stream habitat was also studied in each reach with macroinvertebrates collected from riffles, undercuts, and pools. After a break in sampling, caddisfly larvae increased initially but have declined in the 2 following years, while mayfly nymphs increased in the last 2 years of the study. No significant differences were found in stream quality monitoring (SQM) index scores between the channelized reach compared to upstream and downstream reaches (H = 4.15; p = 0.126). There was a significant difference in taxa richness among pools, riffles, and undercuts (H = 14.09; p < 0.001). A significant difference was also found in the moderately sensitive macroinvertebrates captured in riffles between the channelized, upstream, and downstream reaches (H = 6.82; p = 0.033). A break in sampling resulted in an initial increase in mayfly nymph and caddisfly larvae samples, but it appears a variety of factors may be responsible for the numbers captured. The channelized reach had higher numbers of scuds and crayfish in riffles among the 3 reaches, which may be the result of a change in their distribution related to lack of undercuts. Both scuds and crayfishwere found in significantly greater abundance in undercuts compared to pools and riffles.","PeriodicalId":507306,"journal":{"name":"The Ohio Journal of Science","volume":"56 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139269306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}