Jurnal Teknosains最新文献_第2页

Pengaruh faktor pengali asimetri terhadap kapasitas beban angkat perempuan Indonesia 不对称乘数对印度尼西亚妇女举重能力的影响

Jurnal Teknosains Pub Date : 2023-12-10 DOI: 10.22146/teknosains.82631

Mayesti Kurnianingtias, Ardiyanto Ardiyanto

{"title":"Pengaruh faktor pengali asimetri terhadap kapasitas beban angkat perempuan Indonesia","authors":"Mayesti Kurnianingtias, Ardiyanto Ardiyanto","doi":"10.22146/teknosains.82631","DOIUrl":"https://doi.org/10.22146/teknosains.82631","url":null,"abstract":"Manual material handling can improve occupational diseases such as low back pain and musculoskeletal disorders. In an attempt to reduce the risk of injury as a result of such work, NIOSH published the recommended weight limit (RWL) equation. The RWL formulation was made with Europeans and Americans, so this research was conducted to see the suitability of the RWL formulation for Asians, especially Indonesians. The participants of this study were 30 Indonesian women students. The independent variable in this research is the asymmetry multiplier. The participants perform asymmetric lifting in 0, 30, 60, and 90 degrees with the frequency of 1 lift/min in a 30-minute duration. Other multiplier factors such as the vertical multiplier, distance multiplier, and coupling multiplier are made to have a value of 1. The weight of the load lifted by the respondent is adjusted to the maximum load that each respondent can lift comfortably, which is usually called the maximum acceptable weight of lift (MAWL). The heart rate data used for analysis is the heart rate in the last five minutes. In addition, respondents also filled out the Borg RPE scale. The results of this study were that the heart rate increased from an average resting heart rate of 83.12 beats/minute to 90.6 beats/minute. The statistical test results showed that there was no significant effect between asymmetry lifting tasks on heart rate, energy expenditure, and the Borg's Scale RPE (α = 0,05). Energy expenditure in this study is still below the threshold set by NIOSH. From this study, the predicted AM equation of physiological for Indonesian (energy expenditure) is AM = 1 - (0,0024A) and the predicted AM equation of RPE is AM = 1 - (0,0029A).","PeriodicalId":506417,"journal":{"name":"Jurnal Teknosains","volume":"59 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139184536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Kajian kualitas air dan strategi pengelolaan Rawa Jombor, Klaten, Jawa Tengah 中爪哇克拉登 Rawa Jombor 的水质评估和管理策略

Jurnal Teknosains Pub Date : 2023-12-10 DOI: 10.22146/teknosains.55560

Timotius Ragga Rina, Setyawan Purnama, Andhika Puspito Nugroho

{"title":"Kajian kualitas air dan strategi pengelolaan Rawa Jombor, Klaten, Jawa Tengah","authors":"Timotius Ragga Rina, Setyawan Purnama, Andhika Puspito Nugroho","doi":"10.22146/teknosains.55560","DOIUrl":"https://doi.org/10.22146/teknosains.55560","url":null,"abstract":"Rawa Jombor is a semiartificial reservoir whose contruction are works for serving the needs of irrigation in the dry season and as a floading control in the rainy season. Rawa Jombor also used for fish farming and floating restaurant by the community. The existence of the cages and floating restaurant can have a negative impact to the aquatic environment due to the disposal of waste and leftover food and metabolism that is deposited at the bottom of the water. This study aims to analyze the water quality, pollution levels and to develop a management strategy of Rawa Jombor. Water quality is measured at 9 sampling point determined by purposive sampling based on the use of Rawa Jombor. Pollution level is analized uses pollution index and the management strategy uses analytical Herarchy Process. The results show that Rawa Jombor has decreased water quality marked by several parameters that do not meet the class III of water quality standards according to Government Regulation No. 82 of 2001. Rawa Jombor is included in the category of mildly polluted in areas without activity, floating restaurant and fish cage for water allotment class I, II, III and IV with an average pollution index ranging from 1,02 to 2,886. To maintain the continuity of the Rawa Jombor, a management strategy with priority on the economic aspects is needed with the development of the Rawa Jombor for sustainable fisheries.","PeriodicalId":506417,"journal":{"name":"Jurnal Teknosains","volume":"10 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139184568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of land use changes on the tsunami hazards in part of coastal Kebumen 土地利用变化对克布门市部分沿海地区海啸危害的影响

Jurnal Teknosains Pub Date : 2023-12-10 DOI: 10.22146/teknosains.80954

Bagus Pamungkas, D. Mardiatno, Arry Retnowati

引用次数: 0

Optimized condition for pei-based transient transfection of lifeact-gfp/nls-mcherry expressing plasmid used as cell barcode for syncytia live cell imaging 基于裴氏转染技术瞬时转染用作合胞体活细胞成像细胞条形码的lifeact-gfp/nls-mcherry表达质粒的优化条件

Jurnal Teknosains Pub Date : 2023-12-10 DOI: 10.22146/teknosains.89479

Dennaya Kumara, Hayfa Salsabila Harsan, Metta Novianti, Dinda Lestari, E. P. Septisetyani, P. W. Prasetyaningrum, K. A. Paramitasari, Edy Meiyanto

{"title":"Optimized condition for pei-based transient transfection of lifeact-gfp/nls-mcherry expressing plasmid used as cell barcode for syncytia live cell imaging","authors":"Dennaya Kumara, Hayfa Salsabila Harsan, Metta Novianti, Dinda Lestari, E. P. Septisetyani, P. W. Prasetyaningrum, K. A. Paramitasari, Edy Meiyanto","doi":"10.22146/teknosains.89479","DOIUrl":"https://doi.org/10.22146/teknosains.89479","url":null,"abstract":"The transfection efficiency positively affects the successful plasmid DNA transfer into cells, with the highlight on the amount of plasmid DNA and its ratio to the transfection reagent. Polyethyleneimine (PEI) is a cost-effective transfection reagent that facilitates DNA transfer by forming positively charged DNA complexes. It allows DNA to interact with negatively charged cell surfaces and enter the cells by endocytosis. In this study, we optimized the condition for transient transfection of life act-GFP/NLS-mCherry-expressing plasmid in BHK-21 and 293T cells using PEI. This plasmid is helpful as a biosensor of the cytoskeleton and nucleus that enables live imaging observation using a fluorescence microscope, for instance, in the observation of syncytium. Here, we optimized two independent variables: the amount of DNA (0.5 and 1 µg) and the ratio of DNA-PEI (1:3 and 1:4). GFP and mCherry expressions were observed at 24, 48, and 72 h post-transfection. As a result, transfection efficiency achieved by using PEI in 293T cells is higher than in BHK-21 cells, which are ~90% and ~50%, respectively. Moreover, amongst four different transfection conditions, in both cell lines, 1 µg of plasmid DNA with a 1:3 DNA-PEI ratio yields the most efficiency with the least amount of toxicity. We used this condition for the syncytia observation in 293T cells as a model of the cell-to-cell transmission of SARS-CoV-2. Syncytia formation was successfully observed by detecting the giant cells expressing GFP/mCherry with multiple nuclei.","PeriodicalId":506417,"journal":{"name":"Jurnal Teknosains","volume":"40 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139184371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analisis daya tampung beban pencemaran sungai bedadung Jember menggunakan software wasp 使用黄蜂软件分析 Jember bedadung 河的污染负荷能力

Jurnal Teknosains Pub Date : 2023-12-10 DOI: 10.22146/teknosains.81383

Elida Novita, Moch. Imron Mustofa Bisri, Hendra Pradana

{"title":"Analisis daya tampung beban pencemaran sungai bedadung Jember menggunakan software wasp","authors":"Elida Novita, Moch. Imron Mustofa Bisri, Hendra Pradana","doi":"10.22146/teknosains.81383","DOIUrl":"https://doi.org/10.22146/teknosains.81383","url":null,"abstract":"The distribution of pollution sources from domestic, agricultural, and industrial activities has the potential to reduce the water quality of the Bedadung River, thereby negatively impacting its self-purification. The aim of this research is to model the parameters, specifically the values of Biochemical Oxygen Demand (BOD) and Total Suspended Solid (TSS), and to determine the river's capacity to carry the pollution load using the Water Quality Analysis Simulation Program (WASP). The input parameters for this study include BOD, TSS, and water discharge data collected at 21 monitoring points along the Bedadung River, with the river water sampling technique employing the grab sampling method. The output validation of the modeling is assessed using the Root Mean Square Error (RSME) equation, and the research results indicate that the water quality modeling with WASP software effectively reflects the dynamics of BOD and TSS parameters in the Bedadung River, as supported by an average output validation value of 9%. The pollution load capacity values for the Bedadung River in the Patrang - Ajung segment are found to be 361.48 kg/day for BOD and 5368.31 kg/day for TSS. The positive values suggest that the Bedadung River still has the capacity to naturally degrade organic pollutants.","PeriodicalId":506417,"journal":{"name":"Jurnal Teknosains","volume":"71 1-4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139184661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0