Acta Biomedica Scientifica最新文献

{"title":"Coronaviruses in rodents and insectivores in Altai Republic","authors":"L. Yashina, S. Abramov, N. A. Smetannikova, B. S. Malyshev, A. Krivopalov, T. Dupal","doi":"10.29413/abs.2023-8.6.10","DOIUrl":"https://doi.org/10.29413/abs.2023-8.6.10","url":null,"abstract":"Coronaviruses (family Coronaviridae, genera Alphacoronavirus, Betacoronavirus) are dangerous viral pathogens that have caused three outbreaks of severe respiratory diseases, SARS, MERS, COVID-19. In Russia, data on coronaviruses in natural reservoirs are limited, as investigations began only during the COVID-19 pandemic.The aim. To study the diversity of coronaviruses among rodents and insectivores in the Republic of Altai.Materials and methods. Rodents (n  =  67) and shrews (n  =  52) were captured in 2022. Samples were analyzed by reverse transcription-polymerase chain reaction followed by sequencing.Results and conclusions. Four samples from rodents (Myodes rutilus, M. glareolus, Apodemus peninsulae, A. agrarius) and two samples from an insectivore (Crocidura sibirica) were positive for coronaviruses, among which three different coronaviruses were detected. Rodent-borne coronaviruses are classified in the genus Betacoronavirus, subgenera Embecovirus, and have shown host associated clustering. The nucleotide sequences of Siberian coronaviruses from rodents were identical for closely related species (M. rutilus and M. glareolus, A. agrarius and A. peninsulae) and close (> 94 % homology) to previously published sequences in each of the groups of carriers found in the territory Novosibirsk region, Europe and China. The coronavirus identified from the insectivore, possibly belonging to a new subgenera of the family Coronaviridae, has also been assigned to the genus Betacoronavirus.Conclusion. Five species of natural carriers of three different coronaviruses were detected in the Altai Republic. A high level of identity of coronaviruses genomes from rodents has been revealed, indicating a relatively low rate of their evolution.","PeriodicalId":505136,"journal":{"name":"Acta Biomedica Scientifica","volume":" 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139623241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic heterogeneity of Rickettsia helvetica population","authors":"V. Rar, Y. Igolkina, V. Yakimenko, A. Y. Tikunov, A. Nikitin, T. Epikhina, N. V. Tikunova","doi":"10.29413/abs.2023-8.6.8","DOIUrl":"https://doi.org/10.29413/abs.2023-8.6.8","url":null,"abstract":"Background. To date, the genetic variability of Rickettsia helvetica has not been sufficiently studied.The aim. To study the prevalence and genetic variability of R. helvetica in Ixodes spp. collected in Western Siberia and the Russian Far East.Materials and methods. Ixodes  spp. collected from rodents in the Omsk province, Western Siberia (n = 280) and collected by flagging on Putyatin and Russky Islands in Primorsky Krai, Russian Far East (n = 482) were analyzed for the presence of Rickettsia spp. All positive samples were genotyped for the gltA gene fragment. For a number of R. helvetica samples, fragments of the 16S rRNA, ompA, ompB, sca4, htrA, and groEL genes and 23S–5S intergenic spacer were additionally sequenced.Results. Four Rickettsia species (R. helvetica, “Candidatus Rickettsia tarasevichiae”, “Candidatus Rickettsia uralica”, and “Candidatus Rickettsia mendelii”) were found. Of them, R. helvetica was identified in 72.2 % of Ixodes apronophorus and 18.8 % of  Ixodes trianguliceps from the Omsk province and in single Ixodes persulcatus from the Omsk province and Putyatin Island. This is the first finding of Rickettsia spp. in I. apronophorus. All known R. helvetica sequences from this study and the GenBank database belonged to four well supported monopheletic groups forming genetic lineages I–IV. Lineage I included European isolates from Ixodes ricinus, Western Siberian isolates from I. persulcatus, and some sequences from I. apronophorus. All R. helvetica sequences from I. trianguliceps from the Omsk province and I. persulcatus from  the  Komi Republic and one sequence from I.  apronophorus were assigned to  lineage  II. Most sequences from I.  apronophorus formed lineage  III; all known R. helvetica sequences from I. persulcatus from the Far East formed genetic lineage IV.Conclusion. The genetic heterogeneity of R. helvetica population was first demonstrated. Known isolates of R. helvetica are reliably assigned to four genetic lineages, but not in all cases the association of different lineages with a specific tick species or specific territory was observed.","PeriodicalId":505136,"journal":{"name":"Acta Biomedica Scientifica","volume":" 8","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139623436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The rs11385942 and rs657152 variants are not associated with COVID-19 severity and outcomes in patients treated with favipiravir and remdesivir","authors":"S. Abdullaev, N. Denisenko, I. Temirbulatov, A. Kachanova, S. N. Tuchkova, E. Mikhaylenko, A. V. Kryukov, T. T. Valiev, K. Mirzaev, D. Sychev","doi":"10.29413/abs.2023-8.6.3","DOIUrl":"https://doi.org/10.29413/abs.2023-8.6.3","url":null,"abstract":"Background. There is a mounting evidence in the scientific literature that susceptibility to SARS-CoV-2 infection could vary. The severity of COVID-19 symptoms can  range from asymptomatic to severe respiratory failure, requiring prolonged artificial ventilation. The underlying causes of this range of clinical manifestations remain unclear. Identification of the risk factors that may cause this variation in clinical symptoms is important for identifying the most susceptible populations at highest risk. This should help improve prevention measures, reduce hospitalizations, and decrease the mortality rate of the disease. Previously, an association has been found between the severity of COVID-19 and the genetic markers rs11385942 G>GA and rs657152 A>C.The aim. To assess the impact of carrying polymorphic markers rs11385942 G>GA and rs657152 A>C on the severity of COVID-19 in patients undergoing specific therapy. Materials and methods. A total of 240 patients hospitalized with a coronavirus infection were included in the study. All patients received therapy with favipiravir or remdesivir. The presence of the rs11385942 G>GA and rs657152 A>C variants was determined in all patients. The study compared the length of hospital stays, frequency of patient transfers to the intensive care unit (ICU), and frequency of clinical outcomes (recovery or death) among carriers of allelic variants of the markers under investigation.Results. There were no significant associations between the carriage of variants rs11385942 G>GA and rs657152 A>C and the duration of patients’ hospitalization, frequency of patient transfers to the ICU, and patient outcomes.Conclusion. The carriage of rs11385942 G>GA and rs657152 A>C variants did not affect the severity or type of clinical outcomes in patients with COVID-19.","PeriodicalId":505136,"journal":{"name":"Acta Biomedica Scientifica","volume":" 46","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139623524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of ITGB3, GP1B1, and ITGA2 gene polymorphisms in platelet dysfunction in patients with COVID-19-associated lung damage","authors":"M. V. Osikov, V. N. Antonov, S. Zotov","doi":"10.29413/abs.2023-8.6.2","DOIUrl":"https://doi.org/10.29413/abs.2023-8.6.2","url":null,"abstract":"The aim of the work. To investigate platelet aggregation, polymorphism in the genes that ensure its implementation, and the association between these indicators in patients with COVID-19-associated lung damage, depending on the severity of the clinical course. Methodology. The study involved 75  patients with COVID-19, which, depending on  the severity of lung involvement, were divided into two groups: patients with  damage of up to 50  % of the lung parenchyma (n  =  48) and with damage of more than 50 % (n = 27) respectively. The control group consisted of healthy people (n = 24), comparable in sex and age. In all individuals, the number of platelets, platelet aggregation induced by ADP, collagen and ristomycin were studied; polymorphisms rs6065 in the GP1BA gene, rs1126643 in the ITGA2 gene, and rs5918 in the ITGB3 gene were determined by polymerase chain reaction. The analysis of the obtained data was executed using the IBM SPSS Statistics v. 23 (IMB Corp., USA). Results and discussion. In patients with moderate and severe COVID-19-associated lung damage, platelet aggregation induced by ADP, collagen, and ristomycin accelerated; in severe cases, the number of  platelets decreased. The frequency of variants of the rs6065 polymorphism did not change, the frequency of occurrence of the T/C genotype of the rs5918 polymorphism increased; with moderate severity, the frequency of occurrence of the C/T and T/T genotypes of the rs1126643 polymorphism increased; with severe lung damage, the frequency of occurrence of the mutant C/C genotype polymorphism rs5918 increased. In moderate lung damage, the presence of the  mutant T/T polymorphism rs1126643 accelerated collagen-induced platelet aggregation; in severe cases, the presence of mutant C/C and heterozygous variant C/T polymorphism rs5918 accelerated ADP-induced platelet aggregation. There was no effect of the rs6065 polymorphism on platelet aggregation. The data obtained indicate the possible role of genetic predisposition in the activation of platelet aggregation in patients with COVID-19-associated lung damage.","PeriodicalId":505136,"journal":{"name":"Acta Biomedica Scientifica","volume":" 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139625527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Editor-in-chief’s preface to Issue 6, 2023","authors":"S. Kolesnikov","doi":"10.29413/abs.2023-8.6.1","DOIUrl":"https://doi.org/10.29413/abs.2023-8.6.1","url":null,"abstract":"<jats:p>.</jats:p>","PeriodicalId":505136,"journal":{"name":"Acta Biomedica Scientifica","volume":" 12","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139626617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}