Folia Biologica-Krakow最新文献_第7页

Genetics of T2DM and Its Chronic Complications: Are We Any Closer to the Individual Prediction of Genetic Risk? 2型糖尿病及其慢性并发症的遗传学：我们是否更接近于遗传风险的个体预测？

IF 0.8 4区生物学

Folia Biologica-Krakow Pub Date : 2022-01-01 DOI: 10.14712/fb2022068050159

D Galuška, L Dlouhá, J A Hubáček, K Kaňová

{"title":"Genetics of T2DM and Its Chronic Complications: Are We Any Closer to the Individual Prediction of Genetic Risk?","authors":"D Galuška, L Dlouhá, J A Hubáček, K Kaňová","doi":"10.14712/fb2022068050159","DOIUrl":"10.14712/fb2022068050159","url":null,"abstract":"Type 2 diabetes mellitus (T2DM) is a complex disease that has risen in global prevalence over recent decades, resulting in concomitant and enormous socio-economic impacts. In addition to the well-documented risk factors of obesity, poor dietary habits and sedentary lifestyles, genetic background plays a key role in the aetiopathogenesis of diabetes and the development of associated micro- and macrovascular complications. Recent advances in genomic research, notably next-generation sequencing and genome- wide association studies, have greatly improved the efficiency with which genetic backgrounds to complex diseases are analysed. To date, several hundred single-nucleotide polymorphisms have been associated with T2DM or its complications. Given the polygenic background to T2DM (and numerous other complex diseases), the degree of genetic predisposition can be treated as a \"continuous trait\" quantified by a genetic risk score. Focusing mainly on the Central European population, this review summarizes recent state-of-the-art methods that have enabled us to better determine the genetic architecture of T2DM and the utility of genetic risk scores in disease prediction.","PeriodicalId":50438,"journal":{"name":"Folia Biologica-Krakow","volume":"68 5-6 1","pages":"159-179"},"PeriodicalIF":0.8,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67053932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Up-regulation of MiR-146b-5p Inhibits Fibrotic Lung Pericytes via Inactivation of the Notch1/PDGFRβ/ROCK1 Pathway. MiR-146b-5p上调通过Notch1/PDGFRβ/ROCK1通路失活抑制纤维化肺周细胞

IF 0.8 4区生物学

Folia Biologica-Krakow Pub Date : 2022-01-01 DOI: 10.14712/fb2022068050180

W Shuai, Q Chen, X Zhou

{"title":"Up-regulation of MiR-146b-5p Inhibits Fibrotic Lung Pericytes via Inactivation of the Notch1/PDGFRβ/ROCK1 Pathway.","authors":"W Shuai, Q Chen, X Zhou","doi":"10.14712/fb2022068050180","DOIUrl":"10.14712/fb2022068050180","url":null,"abstract":"Lung fibrosis is a serious human pathology. MiR-146b-5p is down-regulated in idiopathic pulmonary fibrosis, and the Notch1/PDGFRβ/ROCK1 pathway is activated. However, the relation between miR-146b-5p and the Notch1/PDGFRβ/ROCK1 pathway in lung fibrosis remains unclear. To investigate the function of miR-146b-5p in lung fibrosis, an in vivo model of lung fibrosis was established in mice by bleomycin. The fibrosis in lung tissues of mice was observed by HE, Masson and Sirius Red staining. Lung pericytes were isolated and identified by fluorescence microscopy. Immunofluorescence staining and Western blot were used to investigate the expression of desmin, NG2, collagen I and α-SMA. CCK8 assay was used to assess the cell viability, and flow cytometry was performed to evaluate the cell cycle in pericytes. Furthermore, the correlation between miR-146b-5p and Notch1 was analysed by Spearman analysis. The mechanism by which miR-146b-5p affects pericytes and lung fibrosis via the Notch1/ PDGFRβ/ROCK1 pathway was explored by RT-qPCR, Western blot, immunofluorescence staining and dual luciferase reporter gene assay. In bleomycin-treated mice, miR-146b-5p was down-regulated, while Notch1 was up-regulated. Up-regulation of miR-146b-5p significantly inhibited the viability and induced G1 phase arrest of lung pericytes. MiR-146b-5p mimics up-regulated miR-146b-5p, desmin, and NG2 and down-regulated α-SMA and collagen I in the lung pericytes. Additionally, miR-146b-5p was negatively correlated with Notch1, and miR-146b-5p interacted with Notch1. Over-expression of miR-146b-5p inactivated the Notch1/PDGFRβ/ROCK1 pathway. Our results indicate that up-regulation of miR-146b-5p inhibits fibrosis in lung pericytes via modulation of the Notch1/PDGFRβ/ROCK1 pathway. Thus, our study might provide a novel target against lung fibrosis.","PeriodicalId":50438,"journal":{"name":"Folia Biologica-Krakow","volume":"68 5-6 1","pages":"180-188"},"PeriodicalIF":0.8,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67054097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Contribution to the Biogeography of Ablepharus anatolicus and A. budaki (Squamata: Scincidae) Using Ecological Niche Modeling in Turkey 利用生态位模型对土耳其安纳托利库和布达基库生物地理学的贡献

IF 0.7 4区生物学

Folia Biologica-Krakow Pub Date : 2021-12-23 DOI: 10.3409/fb_69-4.17

Muammer Kurnaz, S. S. H. Yousefkhani

{"title":"A Contribution to the Biogeography of Ablepharus anatolicus and A. budaki (Squamata: Scincidae) Using Ecological Niche Modeling in Turkey","authors":"Muammer Kurnaz, S. S. H. Yousefkhani","doi":"10.3409/fb_69-4.17","DOIUrl":"https://doi.org/10.3409/fb_69-4.17","url":null,"abstract":"The Anatolian Peninsula is very richly biodiverse in terms of its location and with new studies, this wealth has gradually increased as new taxa of Anatolian origin are added to the literature. Ablepharus budaki and A. anatolicus, formerly considered to be subspecies of\u0000 A. kitaibelli and A. budaki respectively, are spread throughout the southern part of Anatolia. Although recent phylogenetic and morphological studies revealed their species status, no information was given about the relation of the species with each other in terms of ecological\u0000 niche. In this study, our primary goal was to discover whether the niches of these two taxa were different from each other. Considering the analyses made within the scope of this study, it has been revealed that both A. anatolicus and A. budaki are different from each other in\u0000 terms of their ecological niche. However, since these two taxa have very small contact regions, an example of parapatric speciation, and their distribution areas cover almost completely different geographies, we can say that they have different ecological niche requirements, according to the\u0000 results of this study. As a result, this study supported the findings in literature and the idea that these taxa are two different species","PeriodicalId":50438,"journal":{"name":"Folia Biologica-Krakow","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2021-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49032863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Effects of Salvianolic Acid B/ginsenoside Rg1 Combination Against Chronic Pulmonary Embolism Induced by Polystyrene Microspheres 丹酚酸B/人参皂苷Rg1联合应用对聚苯乙烯微球诱导的慢性肺栓塞的影响

IF 0.7 4区生物学

Folia Biologica-Krakow Pub Date : 2021-12-23 DOI: 10.3409/fb_69-4.20

Huimiao Bian, Na Han, Linlin Wang, Xiao-yu Wang, Kenka Cho, Jun Yin, B. Jiang

{"title":"Effects of Salvianolic Acid B/ginsenoside Rg1 Combination Against Chronic Pulmonary Embolism Induced by Polystyrene Microspheres","authors":"Huimiao Bian, Na Han, Linlin Wang, Xiao-yu Wang, Kenka Cho, Jun Yin, B. Jiang","doi":"10.3409/fb_69-4.20","DOIUrl":"https://doi.org/10.3409/fb_69-4.20","url":null,"abstract":"Pulmonary embolism (PE) is the most life-threatening complication of venous thromboembolism, but few effective treatments have been discovered to attenuate chronic PE currently. In this study, we investigated the protective effects of salvianolic acid B (SalB) and ginsenoside Rg1 (Rg1)\u0000 combination (SalB/Rg1) on chronic PE and explored the potential mechanisms. The PE model was induced by 45 μm polystyrene microspheres and 20 mg/kg of SalB/Rg1 was administered to PE rats intraperitoneally. A histopathological analysis of the lungs and heart was performed through hematoxylin\u0000 and eosin staining and immunohistochemical analysis. The pulmonary index and right ventricular cardiomyocyte cross-sectional area were evaluated. SalB/Rg1 markedly downregulated pulmonary index, attenuated pulmonary interstitial changes, suppressed neutrophil infiltration, prevented collagen\u0000 deposition, and inhibited MMP-9 activities in the lung. We also found that SalB/Rg1 improved right ventricular hypertrophy accompanied by reducing the cardiomyocyte cross-sectional area. These data suggest that SalB/Rg1 played a protective role against microsphere-induced PE and holds a high\u0000 potential for the treatment of PE in the future.","PeriodicalId":50438,"journal":{"name":"Folia Biologica-Krakow","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2021-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48214957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Histological Changes in the Kidneys and Heart in Experimental Acanthamoebiasis in Immunocompetent and Immunosuppressed Hosts 免疫活性和免疫抑制宿主实验性棘阿米巴病肾脏和心脏的组织学变化

IF 0.7 4区生物学

Folia Biologica-Krakow Pub Date : 2021-12-23 DOI: 10.3409/fb_69-4.18

K. Kot, D. Kosik-Bogacka, Łanocha-Arendarczyk, M. Ptak, P. Roszkowska, A. Kram

{"title":"Histological Changes in the Kidneys and Heart in Experimental Acanthamoebiasis in Immunocompetent and Immunosuppressed Hosts","authors":"K. Kot, D. Kosik-Bogacka, Łanocha-Arendarczyk, M. Ptak, P. Roszkowska, A. Kram","doi":"10.3409/fb_69-4.18","DOIUrl":"https://doi.org/10.3409/fb_69-4.18","url":null,"abstract":"The course of Acanthamoeba spp. infection depends on the age and immune status of the host, and the virulence of the Acanthamoeba spp. strain. Some strains of free-living amoebae exhibit organ specificity, during the course of infection, while others may cause changes\u0000 in many organs or completely lose pathogenicity. The aim of the current study was to investigate the pathological properties of Acanthamoeba spp. isolated from a patient with acute myeloid leukemia and atypical pneumonia (AM22). Moreover, the objective was to investigate the histopathological\u0000 changes in the kidneys and heart of immunocompetent and immunosuppressed mice infected with Acanthamoeba spp. Amoebae were re-isolated from both the kidneys and hearts of the inoculated mice, although no cysts or trophozoites of the amoebae were detected in microscopic slides of the\u0000 fragments of these organs. Acanthamoeba spp. induced changes in the kidney and heart weight of infected mice. In immunocompetent and immunosuppressed Acanthamoeba spp. infected mice, we found some histopathological changes, including areas with less acidic cytoplasm and a relaxation\u0000 of muscle fibers. In further studies, it is important to analyze changes in gene and protein expressions in the heart and kidneys of hosts with disseminated acanthamoebiasis to better understand the course of infection in these organs, because the results of histological analysis varied depending\u0000 on the immune status and duration of infection.","PeriodicalId":50438,"journal":{"name":"Folia Biologica-Krakow","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2021-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42433818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Development of Fannia pusio (Diptera: Fanniidae) Under Controlled Temperature Conditions and its Enforcement in the Estimate of the Post-mortem Interval (PMI) 控制温度条件下pusio Fannia（Diptera：Fanniidae）的发育及其在估计死后间隔（PMI）中的应用

IF 0.7 4区生物学

Folia Biologica-Krakow Pub Date : 2021-12-23 DOI: 10.3409/fb_69-4.19

Yolanda Bravo-Pena, J. Galián, E. Romera

{"title":"Development of Fannia pusio (Diptera: Fanniidae) Under Controlled Temperature Conditions and its Enforcement in the Estimate of the Post-mortem Interval (PMI)","authors":"Yolanda Bravo-Pena, J. Galián, E. Romera","doi":"10.3409/fb_69-4.19","DOIUrl":"https://doi.org/10.3409/fb_69-4.19","url":null,"abstract":"Fannia pusio (Wiedemann, 1830) is a species belonging to the family Fanniidae, which is of great forensic, sanitary, and veterinary interest. The behavioral peculiarities of this species, depending on the temperature at which it is found, may provide additional information for\u0000 future research. The application of entomology in the forensic field has focused especially on the early colonizing taxa of corpses that are in the initial stage of decomposition. However, species occurring at more advanced stages can contribute to further knowledge, as is the case with\u0000 F. pusio. In addition, the species has the ability to colonize buried corpses that are inaccessible to larger dipterans. On the other hand, the sanitary and veterinary interest of this species is due to the performance of females as phoretic hosts of Dermatobia hominis eggs that\u0000 cause myiasis in both animals and humans. In the current study, the behavior of F. pusio was observed at a temperature range of 5°C to 40°C. We found that its viability range is limited between 15°C and 35°C; above and below these temperatures, adults survive but oviposition\u0000 does not take place. Data collected by statistical analysis were subsequently applied to calculate the post-mortem interval (PMI) using isomorphen and isomegalen diagrams. The results show a directly proportional relationship between growth rate and temperature increase. However, a slowdown\u0000 in the growth of individuals was observed at extreme temperatures (5°C and 35°C). The results shown in this manuscript, together with the existing bibliography of other species, help to broaden the knowledge of F. pusio, which has not been studied in such depth until now.","PeriodicalId":50438,"journal":{"name":"Folia Biologica-Krakow","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2021-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43603783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

KRAKOW AND ITS RIVERS 克拉科夫和它的河流

IF 0.7 4区生物学

Folia Biologica-Krakow Pub Date : 2021-10-05 DOI: 10.2307/j.ctv201xjkz.5

Andrzej Chwalba

引用次数: 0

PLANTS IN THE LIVES OF MEDIEVAL CRACOVIANS 中世纪恐龙生活中的植物

IF 0.7 4区生物学

Folia Biologica-Krakow Pub Date : 2021-10-05 DOI: 10.2307/j.ctv201xjkz.6

A. Mueller-Bieniek

引用次数: 1

THE POWER OF MYTH: 神话的力量: