Aquaculture Reports最新文献

{"title":"Study on immune response and protective efficacy of Vibrio alginolyticus flagellin FlaD and FlaF subunit vaccine combined with Montanide™ ISA 763B VG adjuvant in pearl gentian grouper (♀ Epinephelus fuscoguttatus × ♂ Epinephelus lanceolatus)","authors":"Bo Liu, X R Wang, Yujuan Li, Xing Long, Yucong Huang, Jichang Jian, Yu Ding","doi":"10.1016/j.aqrep.2026.103377","DOIUrl":"https://doi.org/10.1016/j.aqrep.2026.103377","url":null,"abstract":"Vibrio alginolyticus is a major mariculture pathogen that causes vibriosis and heavy losses in many high-value fish species, such as the pearl gentian grouper. To provide an antibiotic-sparing alternative, we evaluated the immunogenicity and protective efficacy of recombinant flagellin subunits rFlaD and rFlaF, single or formulated with the water-in-oil adjuvant Montanide™ ISA 763B VG for pearl gentian grouper. Recombinant proteins were expressed in Escherichia coli , then purified, and used for intraperitoneal vaccination. At 6-week post-vaccination, the relative percent survival was determined. Immune responses were assessed by measuring serum lysozyme, catalase, and superoxide dismutase activities, quantifying antigen-specific IgM titers via indirect ELISA, and analyzing the expression profiles of immune-related genes. Both antigens conferred protection with relative percent survival values of 62.80 % for rFlaD and 58.67 % for rFlaF, but increased to 79.33 % and 73.43 % in the adjuvant group, respectively. Vaccination elevated the serum lysozyme, catalase, and superoxide dismutase activities, peaking at 3- to 4-week post-vaccination. Antigen-specific serum IgM also rose significantly, with the adjuvanted rFlaD group reaching the highest titer of 1:1280. RT-qPCR showed up-regulation of the immune-related genes in spleen and head kidney, indicating the activation of both innate and adaptive pathways. Collectively, the rFlaD combined with the Montanide™ ISA 763B VG formulation demonstrates strong potential as a subunit vaccine. Its efficacy is augmented by the dual functionality of the flagellin subunit, which acts as both a protective antigen and an inherent immunostimulant, offering a sustainable approach to combat V. alginolyticus in pearl gentian grouper aquaculture. • The vaccine significantly induces the expression of regulatory cytokines. • The adjuvant significantly enhances the immune response. • The rFlaD + Montanide™ ISA 763B VG vaccine formulation is highly effective. • Vibrio alginolyticus flagella acts both as an antigen and as an adjuvant.","PeriodicalId":504152,"journal":{"name":"Aquaculture Reports","volume":"46 1","pages":"103377-103377"},"PeriodicalIF":0.0,"publicationDate":"2026-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147912097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Naringenin alleviates lipid accumulation and inflammation, and promotes hepatic-intestinal health of Largemouth bass (Micropterus salmoides) fed with high-fat diet","authors":"Dexiang Feng, Xiaotong Si, Wenxuan Liu, Pengwei Xun, Qianqian Huang, Mingzhu Liu, Ming Li","doi":"10.1016/j.aqrep.2026.103365","DOIUrl":"https://doi.org/10.1016/j.aqrep.2026.103365","url":null,"abstract":"Naringenin (NGN), a flavonoid compound abundantly found in citrus fruits, exhibits anti-inflammatory, anti-oxidative activities, and hypolipidemic properties. However, nowadays, NGN is mainly used to inhibit the occurrence of viral diseases in aquatic animals by injection as an immunosuppressant, and its safety and other physiological effects as a feed additive have not been sufficiently investigated. This study elucidates how naringenin (NGN) mitigates the negative impacts exerted by a high-fat diet (HFD) on Largemouth bass ( Micropterus salmoides ) from hepatic and intestinal perspectives. After an 8-week feeding trial, the NGN groups had a significant effect on growth performance compared to the HFD group, and significantly decreased the lipid content of the whole body. Secondly, NGN significantly decreased the levels of triglycerides and total cholesterol in serum compared with the HFD group, down-regulated the lipogenesis-related genes, and significantly up-regulated the lipolysis-related genes. Furthermore, hepatic injury markers alanine transaminase and aspartate transaminase were also significantly decreased. Additionally, NGN significantly enhanced the antioxidant capacity of the liver, down-regulated the gene expression of pro-inflammatory factors, and up-regulated the anti-inflammatory factors. There were remarkable changes in the structure of the gut microbiota, where the biomarker of the Na1 group was Dyella , and the main ones in the Na2 group were Asinibacterium , Methylovirgula , Mesorhizobium, and Meiothermus . The function of the gut microbiota in the NGN was more enriched in the fatty acid biosynthesis pathway compared to the high-fat diet. To conclude, the supplementation of NGN to HFD can significantly reduce hepatic lipid deposition and alleviate liver damage, as well as improve gut health status. Among them, the addition of 0.007 % naringenin exhibited a better effect. The results of this study will be used to help reduce the negative effects of an HFD within the aquaculture sector and hold the potential to be regarded as a promising eco-friendly feed additive for aquaculture. • First demonstration of naringenin’s (NGN) hepatoprotective and anti-inflammatory effects in Largemouth bass fed high-fat diet (HFD). • NGN supplementation significantly reduced hepatic lipid accumulation and oxidative stress in HFD-fed fish. • NGN modulated gut microbiota composition, enhancing intestinal health in Largemouth bass. • Providing novel evidence for citrus flavonoids as sustainable functional feed additives in aquaculture. • Offering practical solution to mitigate HFD-induced metabolic disorders in intensive fish farming.","PeriodicalId":504152,"journal":{"name":"Aquaculture Reports","volume":"46 1","pages":"103365-103365"},"PeriodicalIF":0.0,"publicationDate":"2026-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147904810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of temperature on growth performance, antioxidant capacity, ovary development and gene expression in Micropterus salmoides","authors":"Huifen Liu, Yueying Wang, Jiaying Fang, Meng Zhang, Xi Shi, Jianxin Zhang, Ruwei Xu, Xuejun Li, Xianghui Kong","doi":"10.1016/j.aqrep.2025.103255","DOIUrl":"https://doi.org/10.1016/j.aqrep.2025.103255","url":null,"abstract":"Temperature plays a crucial role in the gonadal development and maturation of fish. However, the mechanism by which fish perceive temperature signals and respond accordingly, thereby altering their developmental trajectory, remains incompletely understood. In this study, the largemouth bass (LMB) broodstock were reared at ambient temperature, 18℃, 23℃ and 28℃ for 60 days respectively. The growth differences, antioxidant capacity, serum hormones and ovarian development were compared, and comparative transcriptome analyses of ovarian tissues were performed. The results revealed that the weight gain (WG, %), specific growth rate (SGR, %/day), and gonadosomatic index (GSI, %) of LMB at 18℃ were significantly higher than those in other groups ( p < 0.05). Compared to the control group under natural water temperature, fish maintained at constant temperatures (18 °C, 23 °C and 28 °C) exhibited significantly higher activities of total antioxidant capacity (T-SOD) and catalase (CAT), along with a markedly lower malondialdehyde (MDA) content. Histological analysis showed that under 18℃ and 23℃ conditions, most oocytes were in the vitellogenic stage, while oocytes in the 28℃ group were mainly in the primary growth stage and cortical vesicle stage, with some oocytes atretic. Additionally, transcriptome sequencing detected a total of 30,523 genes, and comparative analysis found 854, 1344, 1823, 322, 1400, and 1349 DEGs in 18℃ vs control, 23℃ vs control, 28℃ vs control, 23℃ vs 18℃, 28℃ vs 18℃, and 28℃ vs 23℃, respectively. KEGG analysis revealed that these DEGs were mainly enriched in pyruvate metabolism, thermogenesis, apelin signaling pathway, PPAR signaling pathway, GnRH signaling pathway, ovarian steroid biosynthesis, PI3K-AKT, TGF-β signaling pathway, prolactin signaling pathway. The results can provide a reference for a deeper understanding of the molecular mechanism by which temperature regulates the gonadal maturation of fish, and also contribute to the analysis of the mechanism of environment-gene interaction. • Growth and antioxidant capacity in largemouth bass are significantly affected by different temperature. • Temperature altered serum hormones and ovary development of largemouth bass broodstock. • Transcriptomics revealed enrichment of pathways in energy metabolism and reproductive regulation under temperature stress.","PeriodicalId":504152,"journal":{"name":"Aquaculture Reports","volume":"45 1","pages":"103255-103255"},"PeriodicalIF":0.0,"publicationDate":"2025-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147919295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of curcumin on growth performance, intestinal morphology and microbiota, and liver transcriptome in Asian yellow pond turtle (Mauremys mutica)","authors":"Mingzuo Gao, Jingu Shi, Zike Yan, Hongyao Zhang, Kaijie Lin, Yutian Zhan, Yuhan Li, Shuyu Han, Jingzhen Liang","doi":"10.1016/j.aqrep.2025.103247","DOIUrl":"https://doi.org/10.1016/j.aqrep.2025.103247","url":null,"abstract":"This study investigated the effects of dietary curcumin supplementation at different concentrations on the growth performance, intestinal morphology and microbiota, and liver transcriptome of Mauremys mutica . Healthy turtles were randomly assigned to five groups: a control group (C0, 0 mg/kg curcumin) and four treatment groups (C100, C200, C400, and C800) receiving curcumin at 100, 200, 400, and 800 mg/kg diet, respectively. Each group consisted of three replicates (30 turtles per replicate), and the trial lasted for 60 days. The results demonstrated that dietary supplementation with 100, 200, and 400 mg/kg curcumin significantly improved both weight gain rate (WGR) and specific growth rate (SGR), with the 200 mg/kg group showing the highest activities of α-amylase, trypsin, and lipase compared to the control group. Curcumin supplementation also significantly increased intestinal villus height, muscular thickness, and goblet cell counts per villus, indicating an improvement in intestinal morphology. Furthermore, curcumin modulated the gut microbiota composition by increasing the relative abundance of Prevotellaceae_unclassified while decreasing Desulfobacterota, Proteobacteria, Edwardsiella , Bacteroides , and Negativibacillus . Transcriptomic analysis of liver tissues revealed 215, 422, 265, and 243 unique DEGs in the 100, 200, 400, and 800 mg/kg groups, respectively. These DEGs were primarily enriched in immune-related pathways such as the PI3K-Akt, and TNF signaling pathways, as well as metabolism-related pathways including the PPAR signaling pathway, nucleotide metabolism, and various amino acid metabolism pathways. Collectively, these findings indicate that curcumin enhances growth performance and digestive capacity, improves intestinal morphology and digestive enzyme activity, modulates microbial community, and regulates hepatic gene expression related to immune and metabolic function in M. mutica . Based on regression analysis (R 2 > 0.5) of key indicators—including WGR, SGR, FCR, and the activities of trypsin and lipase, as well as malondialdehyde (MDA) content—and with no significant effect on survival, the optimal dietary inclusion level of curcumin for M. mutica was estimated to be between 199.74–266.73 mg/kg. • Dietary curcumin significantly enhanced the growth performance and intestinal digestive enzyme activities of Mauremys mutica . • Curcumin modulated the gut microbiota composition and improved intestinal morphology. • Curcumin suppressed pro-inflammatory pathways and enriched metabolism-related pathways.","PeriodicalId":504152,"journal":{"name":"Aquaculture Reports","volume":"45 1","pages":"103247-103247"},"PeriodicalIF":0.0,"publicationDate":"2025-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147920451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hesperetin as a potent antiviral agent against channel catfish virus by interfering with viral internalization and early replication","authors":"Yu Wang, Jie Xu, Fei Yu, Chen Cheng, Kai Hao, Zhe Zhao","doi":"10.1016/j.aqrep.2025.103228","DOIUrl":"https://doi.org/10.1016/j.aqrep.2025.103228","url":null,"abstract":"Channel catfish virus (CCV) is an acute and highly contagious pathogen that easily causes large-scale infections and substantial economic losses to the catfish industry. Currently, there are no commercially available licensed prophylactic vaccines or therapeutic agents specifically developed for the treatment and control of CCV infection. In recent years, the use of natural products with antimicrobial properties has attracted increasing attention in aquaculture. Hesperetin is a plant-derived flavonoid monomer and has a wide range of pharmacology effects. Nevertheless, the antiviral effect of hesperetin against CCV needs to be further elucidated. In this study, we evaluated the antiviral efficacy and potential mechanisms of hesperetin against CCV infection in CCO cells. The results demonstrated that hesperetin possessed remarkable anti-CCV properties, effectively inhibiting viral replication and protecting CCO cells from virus-induced cytopathic effects at non-cytotoxic concentration of 500 μg/ml. Further results revealed that hesperetin significantly suppressed viral gene expression and protein synthesis, reduced progeny virus production, and preserved normal cellular morphology in a dose-dependent manner, based on assessments including quantitative real-time PCR (RT-qPCR), western blot, viral cytopathic effects, viral titer, and plaque formation. Notably, the hesperetin exhibited a 10-fold safety window, with effective antiviral activity at 50 μg/ml well below its tolerated cytotoxicity level. Mechanistically, time-of-addition assays indicated that hesperetin probably acted at the viral entry process and early replication stage. Furthermore, hesperetin exerted its antiviral activity by specifically interfering with the internalization phase of viral entry, which was evidenced by suppression of viral protein expression, viral titer and plaque formation in adsorption and penetration assays. Together, these results identify hesperetin as a promising candidate antiviral agent against CCV infection, which shed light on the development of a novel and potent treatment for herpesvirus infection in aquaculture. • Hesperetin exhibited potent antiviral activity against CCV. • Hesperetin inhibited CCV early replication and blocked viral internalization. • Hesperetin presented a promising sustainable alternative to traditional antiviral drugs in aquaculture.","PeriodicalId":504152,"journal":{"name":"Aquaculture Reports","volume":"45 1","pages":"103228-103228"},"PeriodicalIF":0.0,"publicationDate":"2025-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147914226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of chenodeoxycholic acid inclusion in a plant-based diet on the crosstalk between gut microbiota and bile acids in tilapia","authors":"Jiayuan Jiang, Hancheng Zhao, Li-Xue Dong, Di Peng, Ke Cheng, Yang Liu, Juan Tian, Shan He, Hua Wen, Ming-Dian Liu, Yongju Luo, Zhongbao Guo, Ming Jiang","doi":"10.1016/j.aqrep.2025.103218","DOIUrl":"https://doi.org/10.1016/j.aqrep.2025.103218","url":null,"abstract":"Chenodeoxycholic acid (CDCA), a key component of fish bile acids, has been widely confirmed to promote growth performance and increase the diversity of intestinal flora. This study systematically investigated the effects of dietary CDCA supplementation (75–300 mg/kg) on hepato-intestinal health and bile acid-microbiota interactions in tilapia ( Oreochromis niloticus ) fed plant-based diets. Results revealed that CDCA supplementation significantly reduced hepatic and whole-body lipid content. Furthermore, it decreased serum activities of aspartate transaminase (AST) and alanine transaminase (ALT), while increasing total bilirubin levels. Notably, 300 mg/kg CDCA supplementation significantly reduced serum glucose, triglycerides, low-density lipoprotein cholesterol (LDL-C), total cholesterol (TC), albumin, and total protein levels, indicating improved lipid metabolism and reduced hepatic steatosis. At the molecular level, 75 mg/kg and 150 mg/kg dietary CDCA supplementation downregulated the expression of bile acid metabolism related genes ( fxr ) and upregulated the expression of cyp7a1 . Furthermore, 150 mg/kg dietary CDCA supplementation increased intestinal villus length and muscle layer thickness, as well as increasing the abundance of beneficial bacteria such as Firmicutes, thereby improving the stability of the gut microbiota. Collectively, these results highlight the potential of a dietary supplementation of 150 mg/kg CDCA as a strategic intervention to mitigate the adverse effects of plant-based diets in aquaculture, offering a promising avenue for sustainable and healthy fish production. • Incorporating chenodeoxycholic acid (CDCA) into tilapia’s plant-based diets modulates gallbladder bile acid profiles and alters the composition of their gut microbiota. • CDCA enhances bile acid content by upregulating cyp7a1 through the inhibition of fxr expression, promoting hepatic lipid metabolism and reducing hepatocyte vacuolation. • CDCA boosts beneficial gut bacteria (e.g., Firmicutes, Akkermansia ), reduces fat accumulation, improves intestinal villus height and muscle layer thickness, enhancing tilapia’s overall health.","PeriodicalId":504152,"journal":{"name":"Aquaculture Reports","volume":"45 1","pages":"103218-103218"},"PeriodicalIF":0.0,"publicationDate":"2025-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147880125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Distinct roles of melatonin receptors in spermatogenesis in vitro in four-eyed sleeper (Bostrychus sinensis)","authors":"Jiajun Yao, Haifeng Li, Han Yang, Meisheng Yi, Wei Liu","doi":"10.1016/j.aqrep.2025.103204","DOIUrl":"https://doi.org/10.1016/j.aqrep.2025.103204","url":null,"abstract":"Melatonin is a master neuroendocrine hormone in regulation of circadian rhythm and seasonal breeding behaviors in animal kingdom. Recently, we used the scaffold material to establish the three-dimension (3D) culture method, by which we found that melatonin promotes spermatogenesis in vitro and production of functional sperm in the marine fish four-eyed sleeper ( Bostrychus sinensis ). However, the mechanism how melatonin regulates spermatogenesis in vitro in 3D culture is unclear. In this study, we improved the method by using scaffold-free material spheroid microplate to shorten the time for generation of haploid cells and to yield a higher proportion of haploid sperm, which serves as a helpful tool to study molecular mechanisms underlying spermatogenesis in vitro . In the spheroid microplate, melatonin significantly increased the expression of its membrane receptors ( Mtnr1 , Mtnr2 and Mtnr3 ), suggesting they might play an important role in spermatogenesis. Therefore, we cloned and characterized their expression patterns and found that they were expressed in various types of male germlines in the testis. Knockdown experiments indicated that inhibition of Mtnr1 , Mtnr2 and Mtnr3 resulted in significant reduction of haploid sperms. Whereas, inhibition of Mtnr2 and Mtnr3 rather than Mtnr1 significantly increased and decreased the expression of proliferation‑related genes. Loss-of and gain-of function experiments indicated that they had different effects on downstream testicular development associated signaling. Mtnr1 regulated phosphorylation of ERK1/2, CREB and CAMK2, whereas Mtnr2 affected phosphorylation of CREB and Mtnr3 selectively affected both ERK1/2 and CAMK2, respectively. The present work demonstrated that Mtnrs are important for production of haploid cells and revealed distinct functions in regulation of cell proliferation and downstream signaling, providing insights how melatonin regulates spermatogenesis in vitro and a promising strategy for enhancing the 3D culture system by leveraging the melatonin pathway in turn. • Improving the 3D culture method of fish spermatogenesis in vitro. • Characterizing the expression patterns of four-eyed sleeper Mtnr. • Revealing distinct roles of Mtnr in cell proliferation and meiosis. • Differential regulation of Mtnrs in downstream signaling.","PeriodicalId":504152,"journal":{"name":"Aquaculture Reports","volume":"45 1","pages":"103204-103204"},"PeriodicalIF":0.0,"publicationDate":"2025-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147922463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CRX/NRL/NR2E3-regulated tbx2b integrates BMP2 signaling to control opsin expression and larval feeding in fish","authors":"Ke Lu, Jiaqi Wu, Di‐Mei Xu, Qiuling Wang, Jiahui Duan, Xu‐Fang Liang","doi":"10.1016/j.aqrep.2025.103194","DOIUrl":"https://doi.org/10.1016/j.aqrep.2025.103194","url":null,"abstract":"The T-box transcription factor tbx2b , containing a highly conserved DNA-binding domain, plays a pivotal role in vertebrate visual development, yet its transcriptional regulation remains poorly understood. In this study, we characterized the tbx2b promoter in mandarin fish ( Siniperca chuatsi ), identifying a functional −1189/+269 bp regulatory region. Through comprehensive molecular analyses, we demonstrate that while photoreceptor transcription factors crx , nrl , and nr2e3 all enhance tbx2b promoter activity, only nr2e3 directly binds to the promoter, as confirmed by site-directed mutagenesis and EMSA assays. Intriguingly, BMP2 signaling exhibited dual regulatory effects: while it suppressed tbx2b promoter activity in vitro , in vivo treatment from embryogenesis through 3 days post-hatching (dph) significantly upregulated tbx2b mRNA expression. This treatment also enhanced expression of short-wavelength opsins ( sws1 , sws2Aβ , sws2Aα ) and photoreceptor determinants ( nrl , nr2e3 ), without altering retinal morphology. Beyond photoreceptor development, BMP2 treatment markedly improved first-feeding success, as evidenced by increased food intake, enhanced gut filling, and stimulated locomotor activity with distinct behavioral patterns. Our findings establish a novel regulatory network connecting tbx2b with key photoreceptor transcription factors, and reveal BMP2 as a multifunctional modulator coordinating visual system development with feeding initiation in larval fish. These results provide new insights into the molecular mechanisms governing visual pathway development and its integration with early life history strategies in teleosts. • A tiered regulatory mechanism involves direct tbx2b binding by nr2e3 and indirect enhancement by crx and nrl . • BMP2 signaling upregulates tbx2b and short-wavelength opsins, enhancing photoreceptor differentiation in vivo . • BMP2 boosts larval first-feeding success by stimulating gut filling, food intake, and locomotor activity.","PeriodicalId":504152,"journal":{"name":"Aquaculture Reports","volume":"45 1","pages":"103194-103194"},"PeriodicalIF":0.0,"publicationDate":"2025-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147920428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mitigative effect of chlorogenic acid on nitrite-induced liver injury in grass carp (Ctenopharyngodon idella): Focus on oxidative stress and ferroptosis","authors":"Rong Li, Xiao-Qiu Zhou, Feng Lin, Wei-Dan Jiang, Yang Liu, Yao-Bin Ma, He-Qun Shi, Xiao‐Wan Jin, Hong‐Mei Ren, Lu Zhang, Haifeng Mi, Pei Wu","doi":"10.1016/j.aqrep.2025.103192","DOIUrl":"https://doi.org/10.1016/j.aqrep.2025.103192","url":null,"abstract":"","PeriodicalId":504152,"journal":{"name":"Aquaculture Reports","volume":"45 1","pages":"103192-103192"},"PeriodicalIF":0.0,"publicationDate":"2025-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147892236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A comparison of effects of glycocholic acid, taurocholic acid, and cholic acid on liver health in pearl gentian grouper (Epinephelus fuscoguttatus♂ × E. lanceolatus♀) fed a high-lipid diet","authors":"Jia Xu, Fajun Jiang, Yichao Li, Ermeng Yu, Shiwei Xie, Junxiang Lai, Fan Wang","doi":"10.1016/j.aqrep.2025.103185","DOIUrl":"https://doi.org/10.1016/j.aqrep.2025.103185","url":null,"abstract":"Present study investigated the effects and underlying mechanisms of different primary bile acids (BAs) on liver health in pearl gentian grouper ( Epinephelus fuscoguttatus ♂ × Epinephelus lanceolatus ♀) fed a high-lipid (HL) diet. A total of 375 healthy grouper (15.21 ± 0.05 g) was assigned randomly to 15 tanks, ensuring three replicates per group: control (C), HL, HL with glycocholic acid (GCA), HL with taurocholic acid (TCA), and HL with cholic acid (CA), for 8 weeks. The HL and all BAs treatment did not affect the FBW and WG ( P > 0.05). Compared to C, the HL increased the lipid deposition and the il1β and tnfα expression, decreased the SOD and catalase activities ( P < 0.05). Compared to HL, the GCA increased the lipid deposition, il1β and tnfα expression, malondialdehyde level, and pentanoic acid concentration ( P < 0.05). Compared to HL, the TCA decreased the lipid deposition, il1β and tnfα expression, malondialdehyde level, hexanoic acid and pentanoic acid concentration, increased the SOD and catalase activities ( P < 0.05). Compared to HL, the CA increased the lipid deposition and decreased pentanoic acid concentration ( P < 0.05). Furthermore, all BA treatment groups were found to increase the size of the BA pool. The GCA, TCA, and CA treatments did not induce substantial changes in the structure and composition of the gut microbiota. The composition of SCFAs and BAs is influenced by the dietary intake of different BAs, resulting in distinct microbial-metabolite correlation. In summary, TCA protected grouper form liver impairment induced by a HL diet via improving lipid metabolism and inflammatory responses, and increasing antioxidant capacity. ● HL diet impaired the growth performance and liver health of grouper. ● GCA treatment impaired liver health, TCA improved it and CA not affect it. ● BAs treatments did not induce substantial changes in the gut microbiota. ● The composition of BAs and SCFAs in serum is influenced by the dietary different BAs.","PeriodicalId":504152,"journal":{"name":"Aquaculture Reports","volume":"45 1","pages":"103185-103185"},"PeriodicalIF":0.0,"publicationDate":"2025-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147332021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}