Journal of Buffalo Science最新文献

{"title":"Bovine Tuberculosis Testing in Colombia: Comparative Histopathological, Microbiological, and Molecular Biology Findings","authors":"Paula Palomino Cadavid, D. Balvin, Rafael Villarreal Julio, Enderson Murillo Ramos, Jesus Berdugo Gutierrez, Jhon Didier Ruiz Buitrago, Rene Ramirez Garcia","doi":"10.6000/1927-520x.2024.13.06","DOIUrl":"https://doi.org/10.6000/1927-520x.2024.13.06","url":null,"abstract":"Introduction: Bovine tuberculosis (bTB) is a zoonotic infectious disease present in Colombia, caused by Mycobacterium bovis, and causes tuberculosis in water buffalo (Bubalus bubalis). Diagnosis of bovine tuberculosis through the intradermal test is difficult; evaluating and understanding the behavior of other diagnostic tests is necessary. \u0000Objective: To describe the behavior and results of different diagnostic methods for bovine tuberculosis in water buffalo positive for the Purifed Proteic Derivate (DPP) intradermal test. \u0000Methodology: In water buffaloes positive for comparative cervical tuberculin test, different diagnostic methods were applied, described, and compared: Ziehl-Neelsen staining, microbiological culture, histopathological analysis, and PCR-HRM. \u0000Results: Histopathological tests showed that 26 water buffalo positive for DPP (52%) had histological lesions compatible with bovine tuberculosis. 37% of the evaluated samples from tuberculin-positive Buffalo's lungs and secondary lymph nodes showed acid-alcohol-resistant bacillus with Ziehl-Neelsen staining. Four samples of Mycobacterium bovis from tuberculin-positive buffalo were isolated and identified, with two of these isolates confirmed from tissues with PCR-HRM, and three buffalo with microbiological isolates presented granulomatous lesions through histological analysis. Seventeen tuberculin-positive buffalo (34%) tested positive for real-time PCR HRM, and nine of these buffalo did not have histological lesions compatible with bTB and were confirmed with the molecular test. \u0000Conclusion: Our results provide positive evidence of histological findings, microbiological isolation, and molecular diagnosis of tuberculin-positive water buffalo in the lowlands of Colombia. None of the complementary tests performed showed 100% concordance with the comparative cervical tuberculin test results for bTB.","PeriodicalId":503657,"journal":{"name":"Journal of Buffalo Science","volume":"117 19","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140379445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intradermal Tuberculin Test in Water Buffalo (Bubalus bubalis): Experimental use of Mycobacterial Antigens for the Diagnosis of Bovine Tuberculosis","authors":"A. Martucciello, P. Mazzone, F. Napolitano, J. Bezos, Francesco Grandoni, M. Boniotti, M. Cagiola, G. Cappelli, Gabriele Di Vuolo, Giorgio Galiero, F. Signorelli, E. de Carlo","doi":"10.6000/1927-520x.2024.13.05","DOIUrl":"https://doi.org/10.6000/1927-520x.2024.13.05","url":null,"abstract":"The study aims to evaluate the potential use of mycobacterial ESAT6 and CFP10 antigens, Early Secretory Proteins (ESP) in the Skin Test used for bovine tuberculosis (TB) diagnosis in Water Buffalo. \u0000A pilot study was performed on 21 buffaloes from a TB outbreak and 11 buffaloes from a TB-free herd. Three concentrations of ESAT6-CFP10 (10, 20, and 30 mg) and two of ESP (50 and 100 µg) were inoculated in the Skin Test, along with PPDB, PPDA, and PBS as a negative control. Skin thickness was measured with calipers before the test and every 24 hours for 4 days. Then, to evaluate the specificity of the antigens, a field study was conducted, and 100 buffaloes from a TB-free herd were inoculated using the best antigens concentration derived from the pilot study. \u0000In the positive buffaloes, the strongest skin response was to PPDB at 24h, with some subjects becoming inconclusive at 72 and 96 h. A peak response to PPDA at 48 hours was detected, followed by a slight decrease. The response to ESP-100 µg remained high at 24 and 48 h, then decreased, remaining positive at 72 h. In the 100 TB-free buffaloes, the best specificity was observed using ESAT6-CFP10 and ESP. \u0000ESP yielded the best results, showing higher reactivity in infected animals and no reactivity in the healthy ones at 72 h. Therefore, ESP could be an excellent candidate for further extensive studies in the buffalo species to improve Skin Test performance.","PeriodicalId":503657,"journal":{"name":"Journal of Buffalo Science","volume":"54 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140421466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating the Usability, Perceived Performance, and Perceived Effects of KBGAN iHealth© and KBGAN iFeed© Mobile Apps for Buffalo Management in Selected Municipalities in the Philippines","authors":"E. P. Palacpac, Kae Ann Marie Pacsa Balingit, Airon Andrew Dinulos Bonifacio, Marvin Ardeza Villanueva, Randolph Bautista Tolentino, Mary Rose De Leon Uy-DeGuia, Phoebe Lyndia Tolentino Llantada, Charity Ignacio Castillo, Beverly Janabajal Brul, Hannah Carmela Adecer Rubio, Maica Miclat Abes","doi":"10.6000/1927-520x.2024.13.04","DOIUrl":"https://doi.org/10.6000/1927-520x.2024.13.04","url":null,"abstract":"This study evaluates the KBGAN iHealth© and KBGAN iFeed© mobile apps designed for buffalo health and feeding management, particularly for agricultural extension professionals (AEPs) in selected Philippine municipalities. These apps aim to address challenges in buffalo management, such as limited access to veterinary expertise, personalized recommendations, organized data, communication channels, and difficulties in calculating ideal feed compositions and meeting the distinct needs of smallholder farmers and AEPs. Despite System Usability Scale (SUS) scores indicating marginal acceptability for both apps, weighted mean scores by AEPs for statements assessed on a 5-point Likert scale (1 as strongly disagree and 5 as strongly agree), demonstrate that AEPs reported high confidence in the accuracy of buffalo health diagnostics (Mean of 4.20) and health management recommendations (Mean of 4.17) provided by KBGAN iHealth©. Similarly, KBGAN iFeed© received favorable ratings, with AEPs expressing agreement on the accuracy of feeding recommendations (Mean of 3.89) and the facilitation of feeding ration computations (Mean of 4.00). These positive perceived performance outcomes, coupled with increased confidence (Mean of 4.03) and motivation (Mean of 4.00) among AEPs, suggest the potential for frequent and consistent app usage despite usability concerns. Chi-square tests examining the relationship between AEP characteristics and SUS scores for the apps reveal significant associations between AEPs' education and experience levels and SUS scores for KBGAN iFeed©. The choice of IT device also influences KBGAN iHealth© SUS scores. Proposed enhancements by AEPs include refining algorithms, improving the user interface for navigation, speed, and efficiency, and incorporating features such as photo uploads and geotagging.","PeriodicalId":503657,"journal":{"name":"Journal of Buffalo Science","volume":"3 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139958628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Milk Production, Quality Parameters, and Bacterial Colony Counts of Raw Milk from Murrah Buffaloes Reared in Mixed Crop-Livestock Systems","authors":"Eli Ratni, Elly Roza, Arief Arief","doi":"10.6000/1927-520x.2024.13.03","DOIUrl":"https://doi.org/10.6000/1927-520x.2024.13.03","url":null,"abstract":"This research aimed to assess the quantity and quality of raw Murrah buffalo milk in a mixed crop-livestock farming system in North Sumatra, Indonesia. The study was conducted at the Sumber Ternak Abadi livestock farm in Pagar Merbau District, North Sumatra, Indonesia, established in 2012 within an oil palm plantation. The study observed 40 lactating Murrah buffaloes. Variables included milk production and quality metrics such as total plate count in colony-forming units per ml, water content percentage, total solids, fat content, and pH. Results demonstrated a milk yield standardized to 305 days ranging from 1,200.78±490.25 to 1,505.71±589.73 kg/head/lactation. The total plate count was 2.1 x 105 ± 0.32 CFU/ml, total solids were 16.87% (w/w), fat was 5.7% (v/v), and pH was 6.73. The results confirmed that the raw Murrah buffalo milk from the observed farm meets the Indonesian National Standard for milk quality. A significant positive correlation was found between water content and total bacterial colony count (r = 0.82, p < 0.01), suggesting that higher water content in milk correlates with increased bacterial colonies.","PeriodicalId":503657,"journal":{"name":"Journal of Buffalo Science","volume":"145 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139833579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Milk Production, Quality Parameters, and Bacterial Colony Counts of Raw Milk from Murrah Buffaloes Reared in Mixed Crop-Livestock Systems","authors":"Eli Ratni, Elly Roza, Arief Arief","doi":"10.6000/1927-520x.2024.13.03","DOIUrl":"https://doi.org/10.6000/1927-520x.2024.13.03","url":null,"abstract":"This research aimed to assess the quantity and quality of raw Murrah buffalo milk in a mixed crop-livestock farming system in North Sumatra, Indonesia. The study was conducted at the Sumber Ternak Abadi livestock farm in Pagar Merbau District, North Sumatra, Indonesia, established in 2012 within an oil palm plantation. The study observed 40 lactating Murrah buffaloes. Variables included milk production and quality metrics such as total plate count in colony-forming units per ml, water content percentage, total solids, fat content, and pH. Results demonstrated a milk yield standardized to 305 days ranging from 1,200.78±490.25 to 1,505.71±589.73 kg/head/lactation. The total plate count was 2.1 x 105 ± 0.32 CFU/ml, total solids were 16.87% (w/w), fat was 5.7% (v/v), and pH was 6.73. The results confirmed that the raw Murrah buffalo milk from the observed farm meets the Indonesian National Standard for milk quality. A significant positive correlation was found between water content and total bacterial colony count (r = 0.82, p < 0.01), suggesting that higher water content in milk correlates with increased bacterial colonies.","PeriodicalId":503657,"journal":{"name":"Journal of Buffalo Science","volume":"6 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139774135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}