Frontiers in Bioscience-Elite最新文献

{"title":"Phage Antibodies for Detection of Diagnostically Important Antigens","authors":"OlgaI. Guliy, V. A. Khanadeev, L. Dykman","doi":"10.31083/j.fbe1603024","DOIUrl":"https://doi.org/10.31083/j.fbe1603024","url":null,"abstract":"The need for rapid and cheap synthesis of large numbers of chemical compounds has contributed to the emergence of combinatorial chemistry (simultaneous synthesis of different compounds, in contrast to traditional synthesis, in which each substance is produced individually). Combinatorial library methods were initially applied only to peptides and oligonucleotides. By now, the scope of these libraries has expanded considerably to include proteins, synthetic oligomers, small molecules, and oligosaccharides. The enormous variety of antibodies (Abs) makes it possible to detect clones able to interact highly specifically with almost any natural or synthetic antigen (Ag). Phage Abs are an excellent alternative to mono-and polyclonal Abs, because they are highly stable, have no disulfide bonds, and are much cheaper to make. Monitoring of various substances, including proteins, in a living organism is much in demand. Despite the vast amount of literature available on Ab phage display, the use of phage display to determine diagnostically important Ags has not been sufficiently covered. Many studies have confirmed that unlike other types of Abs, phage Abs ensure highly sensitive Ag detection. Therefore, this review focuses on the use of phage display to prepare Abs specific to diagnostically important Ags (allergens, disease and cancer biomarkers, toxins) and on their application in analytical systems, including biosensors. The use of phage Abs in Ag diagnostics is compared with the use of classical Abs, and the prospects are shown for the use of phage Abs as biosensor sensing elements. This review analyzes the recent advances in the detection of diagnostically important Ags by using phage display–based biosensors. Systematic information is presented about allergens, disease and cancer biomarkers, and toxins detected by using phage Abs. Phage display Abs for sensor-based Ag detection are presented as an affordable alternative to classic tests.","PeriodicalId":502751,"journal":{"name":"Frontiers in Bioscience-Elite","volume":"96 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141657944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rhizobium Inoculant and Seed-Applied Fungicide Effects Improve the Drought Tolerance of Soybean Plants as an Effective Agroecological Solution under Climate Change Conditions","authors":"T. Nyzhnyk, S. Kots, P. Pukhtaievych","doi":"10.31083/j.fbe1603023","DOIUrl":"https://doi.org/10.31083/j.fbe1603023","url":null,"abstract":"Background : Rhizobial inoculation in combination with fungicidal seed treatment is an effective solution for improving soybean resistance to modern climate changes due to the maximum implementation of the plant’s stress-protective antioxidant properties and their nitrogen-fixing potential, which will contribute to the preservation of the environment. Methods : Model ecosystems at different stages of legume–rhizobial symbiosis formation, created by treatment before sowing soybean seeds with a fungicide (fludioxonil, 25 g/L) and inoculation with an active strain of Bradyrhizobium japonicum (titer 10 9 cells per mL), were subjected to microbiological, biochemical, and physiological testing methods in controlled and field conditions. Results : Seed treatment with fungicide and rhizobia showed different patterns in the dynamics of key antioxidant enzymes in soybean nodules under drought conditions. Superoxide dismutase activity increased by 32.7% under moderate stress, while catalase increased by 90.6% under long-term stress. An increase in the antioxidant enzyme activity induced the regulation of lipoperoxidation processes during drought and after the restoration of irrigation. Regeneration after stress was evident in soybean plants with a combination of fungicide seed treatment and rhizobial inoculant, where enzyme levels and lipoperoxidation processes returned to control plant levels. Applying seed treatment with fungicide and Rhizobium led to the preservation of the symbiotic apparatus functioning in drought conditions. As proof of this, molecular nitrogen fixation by nodules has a higher efficiency of 25.6% compared to soybeans without fungicide treatment. In the field, fungicidal treatment of seeds in a complex with rhizobia inoculant induced prolongation of the symbiotic apparatus functioning in the reproductive period of soybean ontogenesis. This positively affected the nitrogen-fixing activity of soybeans during the pod formation stage by more than 71.7%, as well as increasing soybean yield by 12.7% in the field. Conclusions : The application of Rhizobium inoculant and fungicide to seeds contributed to the development of antioxidant protection of soybean plants during droughts due to the activation of key enzymatic complexes and regulation of lipoperoxidation processes, which have a positive effect on nitrogen fixation and productivity of soybeans. This is a necessary element in soybean agrotechnologies to improve plant adaptation and resilience in the context of modern climate change.","PeriodicalId":502751,"journal":{"name":"Frontiers in Bioscience-Elite","volume":"2 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141661584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of a Bacterial Keratinolytic Protease for Effective Degradation of Chicken Feather Waste into Feather Protein Hydrolysates","authors":"Armish Riaz, Hira Muzzamal, Beenish Maqsood, Shumaila Naz, Farooq Latif, Mahjabeen Saleem","doi":"10.31083/j.fbe1603022","DOIUrl":"https://doi.org/10.31083/j.fbe1603022","url":null,"abstract":"Background : Chicken feathers contribute to large quantities of keratinaceous wastes that pose serious environmental problems and must be catered to properly. Chicken feathers are also a potential source of vital proteins, peptides","PeriodicalId":502751,"journal":{"name":"Frontiers in Bioscience-Elite","volume":"121 51","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141666202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dairy-Derived and Bacteriocin-Producing Strain Lactiplantibacillus plantarum LP17L/1: An Assessment of Its Safety and Effect Using Broiler Rabbits","authors":"A. Lauková, L. Chrastinová, I. Plachá, V. Focková, Natália Zábolyová, Eva Bino, Ľ. Grešáková, Rudolf Žitňan, Z. Formelová, J. Ščerbová, G. Bełżecki, R. Miltko, M. Pogány Simonová","doi":"10.31083/j.fbe1603021","DOIUrl":"https://doi.org/10.31083/j.fbe1603021","url":null,"abstract":"Background : Using bacterial (probiotic) strains can influence beneficial health statuses, e","PeriodicalId":502751,"journal":{"name":"Frontiers in Bioscience-Elite","volume":" 23","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141675272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation, Characteristics, and Prospects of Using the Ochrobactrum Intermedium Strain in the Degradation of the Cypermethrin Pesticide","authors":"Diyorbek Kosimov, R. Ergashev, Aziza Mavjudova, N. Lazutin, Tatyana Khegay, Lyudmila Zaynitdinova","doi":"10.31083/j.fbe1602020","DOIUrl":"https://doi.org/10.31083/j.fbe1602020","url":null,"abstract":"","PeriodicalId":502751,"journal":{"name":"Frontiers in Bioscience-Elite","volume":"44 9","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141338723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Komagataella phaffii (Pichia pastoris) as a Powerful Yeast Expression System for Biologics Production","authors":"Yagmur Unver, Ibrahim Dagci","doi":"10.31083/j.fbe1602019","DOIUrl":"https://doi.org/10.31083/j.fbe1602019","url":null,"abstract":"Komagataella phaffii (K. phaffii) ( Pichia pastoris ), also called biotech yeast, is a yeast species with many applications in the biotechnology and pharmaceutical industries. This methylotrophic yeast has garnered significant interest as a platform for the production of recombinant proteins. Numerous benefits include effective secretory expression that facilitates the easy purification of heterologous proteins, high cell density with rapid growth, post-translational changes, and stable gene expression with integration into the genome. In the last thirty years, K. phaffii has also been refined as an adaptable cell factory that can produce hundreds of biomolecules in a laboratory setting and on an industrial scale. Indeed, over 5000 recombinant proteins have been generated so far using the K. phaffii expression method, which makes up 30% of the total cell protein or 80% of the total released protein. K. phaffii has been used to manufacture more than 70 commercial products in addition to over 300 industrial processes that have been granted licenses. Among these are useful enzymes for industrial biotechnology, including xylanase, mannanase, lipase, and phytase. The others are biopharmaceuticals, which include human serum albumin, insulin, hepatitis B surface antigen, and epidermal growth factor. Compared to other expression systems, this yeast is also considered a special host for synthesizing subunit vaccines, which have recently been supplanted by alternative vaccination types, such as inactivated/killed and live attenuated vaccines. Moreover, efficient production of recombinant proteins is achieved through multi-level optimization methods, such as codon bias, gene dosage, promoters, signal peptides, and environmental factors. Therefore, although K. phaffii expression systems are efficient and simple with clearly established process procedures, it is still necessary to determine the ideal conditions since these vary depending on the target protein to ensure the highest recombinant protein generation. This review addresses the K. phaffii expression system, its importance in industrial and biopharmaceutical protein production, and some bioprocessing and genetic modification strategies for efficient protein production. K. phaffii will eventually continue contributing as a potent expression system in research areas and industrial applications.","PeriodicalId":502751,"journal":{"name":"Frontiers in Bioscience-Elite","volume":"82 15","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141353001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of LAB Fermentation on the Nutrient Content, Amino Acid Profile, and Estimated Glycemic Index of Sorghum, Pearl Millet, and Kodo Millet","authors":"D. Mohapatra, Chowdaiah Nickhil, Abhijit Kar, Yogesh Sharma, Sumedha S Deshpande, Manoj Kumar Tripathi, Sadvatha Ramanna Haromuchadi","doi":"10.31083/j.fbe1602018","DOIUrl":"https://doi.org/10.31083/j.fbe1602018","url":null,"abstract":"","PeriodicalId":502751,"journal":{"name":"Frontiers in Bioscience-Elite","volume":"45 22","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141358739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dextran Used in Blood Transfusion, Hematology, and Pharmaceuticals: Biosynthesis of Diverse Molecular-Specification-Dextrans in Enzyme-Catalyzed Reactions","authors":"Divakar Dahiya, Poonam Singh Nigam","doi":"10.31083/j.fbe1602017","DOIUrl":"https://doi.org/10.31083/j.fbe1602017","url":null,"abstract":"","PeriodicalId":502751,"journal":{"name":"Frontiers in Bioscience-Elite","volume":"95 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141388227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Study of the UV Spectral Features in Wine and Their Correlation with Phenolic Constituents","authors":"Joel B. Johnson, Ruslan Timofeev, Anatoliy Kazak, Yurij Grishin, Ludmila Solovyova, Marina Rudenko","doi":"10.31083/j.fbe1602016","DOIUrl":"https://doi.org/10.31083/j.fbe1602016","url":null,"abstract":"","PeriodicalId":502751,"journal":{"name":"Frontiers in Bioscience-Elite","volume":"31 10","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140969151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chitinase Producing Gut-Associated Bacteria Affected the Survivability of the Insect Spodoptera frugiperda","authors":"Thiyagarajan Deborah Winssy, N. O. Gopal, Pandiyan Indiragandhi, Venkatasamy Balasubramani, Rangasamy Anandham","doi":"10.31083/j.fbe1602015","DOIUrl":"https://doi.org/10.31083/j.fbe1602015","url":null,"abstract":"","PeriodicalId":502751,"journal":{"name":"Frontiers in Bioscience-Elite","volume":"58 17","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140971112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}