Archives Animal Breeding最新文献_第3页

Improvement of ram semen quality by luteolin enrichment during cold preservation 在低温保存过程中通过富集叶黄素提高公羊精液质量

Archives Animal Breeding Pub Date : 2024-03-28 DOI: 10.5194/aab-67-123-2024

Sharif Khozein, M. Eslami, Farhad Farrokhi-Ardabili

{"title":"Improvement of ram semen quality by luteolin enrichment during cold preservation","authors":"Sharif Khozein, M. Eslami, Farhad Farrokhi-Ardabili","doi":"10.5194/aab-67-123-2024","DOIUrl":"https://doi.org/10.5194/aab-67-123-2024","url":null,"abstract":"Abstract. The present experiment aimed to investigate the probable protective role of luteolin (Lut) in ram spermatozoa kinematics and the oxidative/anti-oxidative/nitrosative status of semen during cold storage. Ejaculates were collected from five Qezel rams twice a week. Ejaculates were pooled, diluted with Tris–egg yolk extender (negative control) or supplemented with 0 (control; received the solvent of luteolin), 4, 8 and 16 µm Lut. Kinematics parameters, viability and membrane functionality of spermatozoa were assessed. Furthermore, amounts of malondialdehyde (MDA) and anti-oxidant activity (AOA), superoxide dismutase activity (SOD) and total nitrate nitrite (TNN) were evaluated in the medium (diluent) and spermatozoa, separately, at 0, 24, 48 and 72 h after storage at 4 °C. Percentages of forward progressive motility and membrane integrity were significantly higher in 8 and 16 µm groups compared to control groups at 72 h (P<0.05). No significant differences were observed in viability among groups during the study (P>0.05). Lower MDA contents were observed in medium and spermatozoa of 8 and 16 µm treated groups compared to controls at 72 h (P<0.05). In addition, higher AOA levels were observed in the medium of Lut-treated groups compared to controls at 48 and 72 h (P<0.05). The activity of SOD was improved by luteolin addition. Luteolin enrichment did not affect TNN amounts. It seems that luteolin (at 8 and 16 µm) as a flavonoid protects the ram semen by its anti-oxidative properties and by reduction of lipid peroxidation following 48 and 72 h storage.\u0000","PeriodicalId":502344,"journal":{"name":"Archives Animal Breeding","volume":"38 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140371895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of protected fatty acid supplementation on ovarian activity, reproductive hormone profiles and reproduction of Barki ewes under semiarid conditions 补充受保护脂肪酸对半干旱条件下巴基母羊卵巢活动、生殖激素谱和繁殖的影响

Archives Animal Breeding Pub Date : 2024-03-14 DOI: 10.5194/aab-67-111-2024

B. Farrag, Khalid Ahmed El-Bahrawy, H. A. Shedeed, Muhammed Ahmed-Hilmy El-Rayes

{"title":"Effect of protected fatty acid supplementation on ovarian activity, reproductive hormone profiles and reproduction of Barki ewes under semiarid conditions","authors":"B. Farrag, Khalid Ahmed El-Bahrawy, H. A. Shedeed, Muhammed Ahmed-Hilmy El-Rayes","doi":"10.5194/aab-67-111-2024","DOIUrl":"https://doi.org/10.5194/aab-67-111-2024","url":null,"abstract":"Abstract. The objective of this experiment was to evaluate the impact of dietary supplementation with rumen-protected fat (calcium soaps of fatty acids, CSFA) and/or hormonal superovulation using equine chorionic gonadotropin (eCG) on ovarian activity, changes in ovarian hormones, and reproductive performance in Barki ewes under semiarid conditions. A total of 39 non-pregnant Barki ewes, with an average body weight of 34.7 ± 0.25 kg and an average age of 3–4 years, were divided into four groups. The first group (9 ewes) served as the control, while the second group (10 ewes) received double doses of PGF2α, administered 9 d apart, and was superovulated with 600 IU (international unit) eCG. The third group (10 ewes) was supplemented with 30 g per head per day of rumen-protected fat. The fourth group (10 ewes) underwent the same superovulation protocol as the second group and was also supplemented with 30 g per head per day of rumen-protected fat. All the ewes were fed a basal diet. Ovarian activity was detected using ultrasonography during the estrus phase in all the groups, after which all the ewes were naturally bred with Barki rams. The results demonstrated significant (P<0.05) increases in estradiol 17-β (E2) and progesterone (P4) levels in the treated groups compared to the control group. Ewes in all the treated groups exhibited large follicles, with an overall mean diameter (≥5 mm) significantly higher than that of ewes in the control group. Although there were no significant differences between the treated and control groups, the ewes treated with CSFA and/or the eCG hormone showed tendencies to improve the reproductive traits of Barki ewes. Overall, the findings of this study demonstrated a positive effect of CSFA alone or in combination with an estrous synchronization protocol on ovarian activity in Barki ewes, as indicated by the number and size of follicles and the presence of large-sized follicles.\u0000","PeriodicalId":502344,"journal":{"name":"Archives Animal Breeding","volume":"10 12","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140244222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Screening and bioinformatics analysis of a potential ceRNA network in melatonin-induced cashmere growth in Liaoning cashmere goats 辽宁绒山羊褪黑素诱导羊绒生长的潜在ceRNA网络筛选与生物信息学分析

Archives Animal Breeding Pub Date : 2024-02-21 DOI: 10.5194/aab-67-97-2024

Mei Jin, Weiyu Fan, Suhe Lyu, Linlin Cong, Tianwei Xue

{"title":"Screening and bioinformatics analysis of a potential ceRNA network in melatonin-induced cashmere growth in Liaoning cashmere goats","authors":"Mei Jin, Weiyu Fan, Suhe Lyu, Linlin Cong, Tianwei Xue","doi":"10.5194/aab-67-97-2024","DOIUrl":"https://doi.org/10.5194/aab-67-97-2024","url":null,"abstract":"Abstract. The purpose of this study was to investigate the effect of melatonin (MT) on the expression patterns of lncRNA, mRNA and miRNA in Liaoning cashmere goat (LCG) skin fibroblasts. A quantity of 200 ng L−1 MT (MT group) stimulated LCG skin fibroblasts for 48 h, and RNA sequencing was conducted with the control group (Con group) (n=3). The ceRNA network was constructed by bioinformatics analysis of the sequencing data and transmission electron microscopy observation of coated pits and endocytic vesicles. In this study, the results indicated that MT treatment significantly facilitated the proliferation of LCG skin fibroblasts and increased the number of coated pits and vesicles. A total of 775 mRNAs, 57 lncRNAs and 10 miRNAs had differential expression, as indicated by RNA sequencing of skin fibroblasts administrated on the MT group and Con group. The regulatory network of ceRNA was studied, and the results suggested that inositol phosphate metabolism, the cGMP–PKG signaling pathway, endocytosis and other pathways played a certain role in the growth and development of the LCG cashmere. Moreover, the key genes (e.g., CREB1, PIK3C3, AGAP3, MEF2A, ASAP2, IRAG1, PNISR, PIP5K1A, SRSF11, ZRANB2, RBM39 and CBL) were regulated by chi-miR-34c-5p, chi-miR-34c-3p and chi-miR-195-5p. The above mRNAs were competitively bound by 15 lncRNAs (e.g., MSTRG.28630.12, MSTRG.28660.14, MSTRG.28099.7). And through dual luciferase and other experiments, it was further confirmed that PIP5K1A is the target gene of miR-34c-5p. This finding provides new insights into the molecular mechanism by which melatonin promotes villi growth in cashmere.\u0000","PeriodicalId":502344,"journal":{"name":"Archives Animal Breeding","volume":"26 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140443078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Integrating dietary supplementation with poppy (Papaver somniferum L.) seed meal: effects on growth performance, nutrient digestibility, and faecal microbiota in broilers 在日粮中添加罂粟（Papaver somniferum L.）籽粉：对肉鸡生长性能、营养消化率和粪便微生物群的影响

Archives Animal Breeding Pub Date : 2024-02-15 DOI: 10.5194/aab-67-73-2024

Riaz Khan, Muhammad Tahir, S. Naz, I. Alhidary, Shamsuddin Shamsi, Sifa Dai, Rifat Ullah Khan, V. Tufarelli

{"title":"Integrating dietary supplementation with poppy (Papaver somniferum L.) seed meal: effects on growth performance, nutrient digestibility, and faecal microbiota in broilers","authors":"Riaz Khan, Muhammad Tahir, S. Naz, I. Alhidary, Shamsuddin Shamsi, Sifa Dai, Rifat Ullah Khan, V. Tufarelli","doi":"10.5194/aab-67-73-2024","DOIUrl":"https://doi.org/10.5194/aab-67-73-2024","url":null,"abstract":"Abstract. The study investigated the effects of supplementing broiler diets with poppy (Papaver somniferum L.) seed meal (PSM) on growth performance, nutrient digestibility, faecal microbiota, and blood profiles. A total of 250 unsexed 1 d old broilers were allocated to five distinct treatment groups. PSM was incorporated into the diets at varying levels: 0 % (PSM0), 5 % (PSM5), 10 % (PSM10), 15 % (PSM15), and 20 % (PSM20). The findings indicated that growth performance, including weight gain, feed intake, and feed conversion ratio (FCR), was significantly improved (P<0.05) in the PSM20 group compared to the control. However, carcass weight experienced a notable decrease (P<0.05) in the PSM20 group. Regarding nutrient digestibility, PSM supplementation led to reduced crude protein digestibility. Nevertheless, apparent metabolizable energy and ash content were significantly enhanced (P<0.05) in the PSM15 and PSM20 groups. Notably, faecal microbiota also experienced substantial improvement (P<0.05) in the PSM20 group. In conclusion, the study demonstrates that incorporating poppy seed meal at a rate of 20 % in broiler diets enhances growth performance, improves nutrient digestibility, and positively influences faecal microbiota composition.\u0000","PeriodicalId":502344,"journal":{"name":"Archives Animal Breeding","volume":"60 22","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139775158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Association analysis of single nucleotide polymorphisms in the sheep FecB gene with reproductive and body size performance 绵羊 FecB 基因单核苷酸多态性与繁殖和体型表现的关联分析

Archives Animal Breeding Pub Date : 2024-02-15 DOI: 10.5194/aab-67-81-2024

Lingchao Kong, Shuaitong Li, Yuan Pan, Jiaqi Li, Siyi Li, Yining Liu, Sibing Hou, Qingkun Liu, Yanjun Qiao, Yinggang Sun, Zeying Wang

{"title":"Association analysis of single nucleotide polymorphisms in the sheep FecB gene with reproductive and body size performance","authors":"Lingchao Kong, Shuaitong Li, Yuan Pan, Jiaqi Li, Siyi Li, Yining Liu, Sibing Hou, Qingkun Liu, Yanjun Qiao, Yinggang Sun, Zeying Wang","doi":"10.5194/aab-67-81-2024","DOIUrl":"https://doi.org/10.5194/aab-67-81-2024","url":null,"abstract":"Abstract. The FecB gene is one of those responsible for regulating reproductive traits in sheep. This experiment was designed to explore the impact of SNPs (single nucleotide polymorphisms) within the FecB gene on both the reproductive and body size performance of sheep. In this experiment, two SNP sites were identified as C413217T and A431965G. Through analysis of genetic diversity and correlations, we aimed to identify combinations of genotypes and haplotypes that influence reproductive performance and body size performance. The most prominent genotypes associated with lambing performance were identified as AA and CT, with the dominant haplotype combination being AACT. For body size performance, the dominant genotypes were AA and CC, while the dominant haplotype combination was AACC. These advantageous genotypes and haplotype combinations are conducive to promoting the selection and improvement of sheep breeds, advancing the progress of sheep genetic breeding, and providing theoretical support for the breeding of higher-fertility sheep.\u0000","PeriodicalId":502344,"journal":{"name":"Archives Animal Breeding","volume":"153 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139835807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Association analysis of single nucleotide polymorphisms in the sheep FecB gene with reproductive and body size performance 绵羊 FecB 基因单核苷酸多态性与繁殖和体型表现的关联分析

Archives Animal Breeding Pub Date : 2024-02-15 DOI: 10.5194/aab-67-81-2024

Lingchao Kong, Shuaitong Li, Yuan Pan, Jiaqi Li, Siyi Li, Yining Liu, Sibing Hou, Qingkun Liu, Yanjun Qiao, Yinggang Sun, Zeying Wang

{"title":"Association analysis of single nucleotide polymorphisms in the sheep FecB gene with reproductive and body size performance","authors":"Lingchao Kong, Shuaitong Li, Yuan Pan, Jiaqi Li, Siyi Li, Yining Liu, Sibing Hou, Qingkun Liu, Yanjun Qiao, Yinggang Sun, Zeying Wang","doi":"10.5194/aab-67-81-2024","DOIUrl":"https://doi.org/10.5194/aab-67-81-2024","url":null,"abstract":"Abstract. The FecB gene is one of those responsible for regulating reproductive traits in sheep. This experiment was designed to explore the impact of SNPs (single nucleotide polymorphisms) within the FecB gene on both the reproductive and body size performance of sheep. In this experiment, two SNP sites were identified as C413217T and A431965G. Through analysis of genetic diversity and correlations, we aimed to identify combinations of genotypes and haplotypes that influence reproductive performance and body size performance. The most prominent genotypes associated with lambing performance were identified as AA and CT, with the dominant haplotype combination being AACT. For body size performance, the dominant genotypes were AA and CC, while the dominant haplotype combination was AACC. These advantageous genotypes and haplotype combinations are conducive to promoting the selection and improvement of sheep breeds, advancing the progress of sheep genetic breeding, and providing theoretical support for the breeding of higher-fertility sheep.\u0000","PeriodicalId":502344,"journal":{"name":"Archives Animal Breeding","volume":"8 8","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139776172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrating dietary supplementation with poppy (Papaver somniferum L.) seed meal: effects on growth performance, nutrient digestibility, and faecal microbiota in broilers 在日粮中添加罂粟（Papaver somniferum L.）籽粉：对肉鸡生长性能、营养消化率和粪便微生物群的影响

Archives Animal Breeding Pub Date : 2024-02-15 DOI: 10.5194/aab-67-73-2024

Riaz Khan, Muhammad Tahir, S. Naz, I. Alhidary, Shamsuddin Shamsi, Sifa Dai, Rifat Ullah Khan, V. Tufarelli

{"title":"Integrating dietary supplementation with poppy (Papaver somniferum L.) seed meal: effects on growth performance, nutrient digestibility, and faecal microbiota in broilers","authors":"Riaz Khan, Muhammad Tahir, S. Naz, I. Alhidary, Shamsuddin Shamsi, Sifa Dai, Rifat Ullah Khan, V. Tufarelli","doi":"10.5194/aab-67-73-2024","DOIUrl":"https://doi.org/10.5194/aab-67-73-2024","url":null,"abstract":"Abstract. The study investigated the effects of supplementing broiler diets with poppy (Papaver somniferum L.) seed meal (PSM) on growth performance, nutrient digestibility, faecal microbiota, and blood profiles. A total of 250 unsexed 1 d old broilers were allocated to five distinct treatment groups. PSM was incorporated into the diets at varying levels: 0 % (PSM0), 5 % (PSM5), 10 % (PSM10), 15 % (PSM15), and 20 % (PSM20). The findings indicated that growth performance, including weight gain, feed intake, and feed conversion ratio (FCR), was significantly improved (P<0.05) in the PSM20 group compared to the control. However, carcass weight experienced a notable decrease (P<0.05) in the PSM20 group. Regarding nutrient digestibility, PSM supplementation led to reduced crude protein digestibility. Nevertheless, apparent metabolizable energy and ash content were significantly enhanced (P<0.05) in the PSM15 and PSM20 groups. Notably, faecal microbiota also experienced substantial improvement (P<0.05) in the PSM20 group. In conclusion, the study demonstrates that incorporating poppy seed meal at a rate of 20 % in broiler diets enhances growth performance, improves nutrient digestibility, and positively influences faecal microbiota composition.\u0000","PeriodicalId":502344,"journal":{"name":"Archives Animal Breeding","volume":"202 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139834529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Associations of bovine beta-casein and kappa-casein genotypes with genomic merit in Holstein Friesian cattle 荷斯坦弗里斯兰牛的β-酪蛋白和卡巴酪蛋白基因型与基因组优劣的关系

Archives Animal Breeding Pub Date : 2024-02-14 DOI: 10.5194/aab-67-61-2024

S. Ardıçlı, Özgür Aldevir, Emrah Aksu, Kerem Kucuk, Ahmet Gümen

{"title":"Associations of bovine beta-casein and kappa-casein genotypes with genomic merit in Holstein Friesian cattle","authors":"S. Ardıçlı, Özgür Aldevir, Emrah Aksu, Kerem Kucuk, Ahmet Gümen","doi":"10.5194/aab-67-61-2024","DOIUrl":"https://doi.org/10.5194/aab-67-61-2024","url":null,"abstract":"Abstract. The relationship between the index values used to evaluate the genomic value and essential markers such as casein genes provides important information at the herd level. Therefore, this study aimed to determine the association between casein gene genotypes and the indices of genetic merit in Holstein Friesian cattle. A total of 805 cows were genotyped using the Affymetrix® Axiom® array system. We used data composed of the total performance index (TPI) and net merit (NM) values as well as the predicted transmitting abilities (PTAs) indices, including milk, fat, fat percentage, protein, protein percentage, combined fat and protein (CFP), productive life (PL), somatic cell score (SCS), daughter pregnancy rate (DPR), livability (LV), udder composite (UDC), and feet–legs composite (FLC) of each animal. The statistical analysis consisted of a one-way analysis of variance (ANOVA) test followed by Tukey's test. The A2A2 and the AB genotypes were predominant in the CSN2 and CSN3 genes, respectively. The A2A2 animals were found to have higher TPI and NM values. Moreover, they exhibited higher PTA values for proteins, CFP, and PL. On the other hand, the A1A1 genotype was significantly associated with the highest UDC. Concerning the CSN3 haplotypes, the BB animals had higher protein percentage and PL than alternative haplotypes. The AA and AB haplotypes were found to be significantly associated with the highest SCS and DPR values, respectively. In addition, the BE haplotype had the highest NM. Selection procedures focusing on casein genes in dairy cattle are becoming increasingly common worldwide, especially for A2 milk. However, herd-based dynamics are also fundamental to providing a desired genetic merit for the animals. This study may be valuable for further analyses regarding selection decisions using the breeding values of candidate animals in commercial dairy herds.\u0000","PeriodicalId":502344,"journal":{"name":"Archives Animal Breeding","volume":"29 25","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139778371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Associations of bovine beta-casein and kappa-casein genotypes with genomic merit in Holstein Friesian cattle 荷斯坦弗里斯兰牛的β-酪蛋白和卡巴酪蛋白基因型与基因组优劣的关系

Archives Animal Breeding Pub Date : 2024-02-14 DOI: 10.5194/aab-67-61-2024

S. Ardıçlı, Özgür Aldevir, Emrah Aksu, Kerem Kucuk, Ahmet Gümen

{"title":"Associations of bovine beta-casein and kappa-casein genotypes with genomic merit in Holstein Friesian cattle","authors":"S. Ardıçlı, Özgür Aldevir, Emrah Aksu, Kerem Kucuk, Ahmet Gümen","doi":"10.5194/aab-67-61-2024","DOIUrl":"https://doi.org/10.5194/aab-67-61-2024","url":null,"abstract":"Abstract. The relationship between the index values used to evaluate the genomic value and essential markers such as casein genes provides important information at the herd level. Therefore, this study aimed to determine the association between casein gene genotypes and the indices of genetic merit in Holstein Friesian cattle. A total of 805 cows were genotyped using the Affymetrix® Axiom® array system. We used data composed of the total performance index (TPI) and net merit (NM) values as well as the predicted transmitting abilities (PTAs) indices, including milk, fat, fat percentage, protein, protein percentage, combined fat and protein (CFP), productive life (PL), somatic cell score (SCS), daughter pregnancy rate (DPR), livability (LV), udder composite (UDC), and feet–legs composite (FLC) of each animal. The statistical analysis consisted of a one-way analysis of variance (ANOVA) test followed by Tukey's test. The A2A2 and the AB genotypes were predominant in the CSN2 and CSN3 genes, respectively. The A2A2 animals were found to have higher TPI and NM values. Moreover, they exhibited higher PTA values for proteins, CFP, and PL. On the other hand, the A1A1 genotype was significantly associated with the highest UDC. Concerning the CSN3 haplotypes, the BB animals had higher protein percentage and PL than alternative haplotypes. The AA and AB haplotypes were found to be significantly associated with the highest SCS and DPR values, respectively. In addition, the BE haplotype had the highest NM. Selection procedures focusing on casein genes in dairy cattle are becoming increasingly common worldwide, especially for A2 milk. However, herd-based dynamics are also fundamental to providing a desired genetic merit for the animals. This study may be valuable for further analyses regarding selection decisions using the breeding values of candidate animals in commercial dairy herds.\u0000","PeriodicalId":502344,"journal":{"name":"Archives Animal Breeding","volume":"63 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139838297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Polymorphisms of the BMPR1B, BMP15 and GDF9 fecundity genes in four Chinese sheep breeds 中国四个绵羊品种的 BMPR1B、BMP15 和 GDF9 育性基因的多态性

Archives Animal Breeding Pub Date : 2024-02-07 DOI: 10.5194/aab-67-51-2024

Jinxin Wang, Yufang Liu, Siwu Guo, R. Di, Xiangyu Wang, Xiaoyun He, Mingxing Chu

{"title":"Polymorphisms of the BMPR1B, BMP15 and GDF9 fecundity genes in four Chinese sheep breeds","authors":"Jinxin Wang, Yufang Liu, Siwu Guo, R. Di, Xiangyu Wang, Xiaoyun He, Mingxing Chu","doi":"10.5194/aab-67-51-2024","DOIUrl":"https://doi.org/10.5194/aab-67-51-2024","url":null,"abstract":"Abstract. Numerous studies on prolific sheep breeds have shown that the transforming growth factor beta (TGF-β) superfamily members, including bone morphogenetic protein receptor type 1B (BMPR1B), bone morphogenetic protein 15 (BMP15) and growth differentiation factor 9 (GDF9), are the essential regulators of ovulation rate and litter size. In total, 11 known mutations (1 in BMPR1B, 6 in BMP15 and 4 in GDF9) are able to increase the ovulation rate and litter size, respectively. In this study, the genomic DNA was isolated from 512 high-prolificacy sheep (including the Small-tailed Han, Hu and Wadi sheep breeds) and 164 low-prolificacy sheep (Tan sheep), and genotyping of the specific mutations of the three fecundity-related genes was carried out by sequencing. The results showed that the FecB mutation in BMPR1B was detected in all four sheep breeds, and the frequency of B allele was significantly higher in the high-prolificacy breeds than that in the low-prolificacy breed (P<0.001). A novel mutation, c.T755C (named S1), was found in BMP15 from the four sheep breeds. However, known mutations such as FecXI, FecXH, FecXB, FecXG, FecXL and FecXR were not detected in these breeds. Three known loci (G1, G3 and G4) and a new mutation, c.A1515G (named S2), were found in GDF9, and the other three known mutations (FecGH (G8), FecGE and FecTT) were not detected in all four sheep breeds. The genotype distribution at the G1 and G4 loci had significant differences between the low-prolificacy sheep breed and the other three high-prolificacy sheep breeds. There was no difference in the genotype distribution at the G1 and G4 loci between the three high-prolificacy sheep breeds. Haplotype analysis of the four polymorphic loci in GDF9 suggested that H4 (GGAA) was the preponderant haplotype in the three high-prolificacy sheep breeds, but H1 (GGGG) was in the low-prolificacy sheep breed. These results preliminarily showed that the BMPR1B and GDF9 might be major genes influencing the prolificacy of Chinese sheep breeds.\u0000","PeriodicalId":502344,"journal":{"name":"Archives Animal Breeding","volume":"29 16","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139795720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0