Antonie van Leeuwenhoek最新文献

Azotosporobacter soli gen. nov., sp. nov., a novel nitrogen-fixing bacterium isolated from paddy soil 从稻田土壤中分离出的新型固氮菌--Azotosporobacter soli gen.

Antonie van Leeuwenhoek Pub Date : 2024-05-17 DOI: 10.1007/s10482-024-01978-6

Cheng-Jie Xie, Ling Yao, R. Tang, Shuang Han, Shang Yang, H. Alwathnani, Christopher Rensing, Guo-Hong Liu, Shunhua Zhou

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Extracellular vesicles from the mycoparasitic fungus Trichoderma harzianum 毛霉的胞外囊泡

Antonie van Leeuwenhoek Pub Date : 2024-04-02 DOI: 10.1007/s10482-024-01958-w

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Phylogenomic analysis of the Ponticus clade: strains isolated from the spotted rose snapper (Lutjanus guttatus) Ponticus 支系的系统进化分析：从斑点玫瑰鲷（Lutjanus guttatus）中分离出的菌株

Antonie van Leeuwenhoek Pub Date : 2024-03-20 DOI: 10.1007/s10482-024-01959-9

Alejandra de Jesús Chavarín-Meza, Bruno Gómez-Gil, Adrián González-Castillo

{"title":"Phylogenomic analysis of the Ponticus clade: strains isolated from the spotted rose snapper (Lutjanus guttatus)","authors":"Alejandra de Jesús Chavarín-Meza, Bruno Gómez-Gil, Adrián González-Castillo","doi":"10.1007/s10482-024-01959-9","DOIUrl":"https://doi.org/10.1007/s10482-024-01959-9","url":null,"abstract":"The family Vibrionaceae is classified into many clades based on their phylogenetic relationships. The Ponticus clade is one of its clades and consists of four species, Vibrio panuliri, V. ponticus, V. rhodolitus, and V. taketomensis. Two strains, CAIM 703 and CAIM 1902, were isolated from the diseased spotted rose snapper external lesion (Lutjanus guttatus), they were analyzed to determine their taxonomic position, a phylogenetic analysis was performed based on the 16S rRNA sequences proved that the two strains are members of the genus Vibrio and they belong to the Ponticus clade. Then, a phylogenomic analysis was performed with four type strains and four reference strains isolated from marine organisms and aquatic environments. Multilocus Sequence Analysis (MLSA) of 139 single-copy genes showed that CAIM 703 and CAIM 1902 belong to V. panuliri. The 16S rRNA sequence similarity value between CAIM 703 and CAIM 1902 was 99.61%. The Ponticus clade species showed Average Nucleotide Identity (ANI) values between 78 to 80% against the two strains for ANIb, except V. panuliri LBS2T (99% and 100% similarity). Finally, this analysis represents the first phylogenomic analysis of the Ponticus clade where V. panuliri strains are reported from Mexico.","PeriodicalId":501687,"journal":{"name":"Antonie van Leeuwenhoek","volume":"151 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140171139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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The role of the Flb protein family in the life cycle of Aspergillus niger Flb 蛋白家族在黑曲霉生命周期中的作用

Antonie van Leeuwenhoek Pub Date : 2024-03-19 DOI: 10.1007/s10482-024-01957-x

Xiaoyi Chen, Juan P. Moran Torres, Han A. B. Wösten

{"title":"The role of the Flb protein family in the life cycle of Aspergillus niger","authors":"Xiaoyi Chen, Juan P. Moran Torres, Han A. B. Wösten","doi":"10.1007/s10482-024-01957-x","DOIUrl":"https://doi.org/10.1007/s10482-024-01957-x","url":null,"abstract":"Genes flbA-E are involved in sporulation and vegetative growth in Aspergillus nidulans. Inactivation of either of these genes results in a fluffy phenotype with delayed or even abolished sporulation. Previously, a non-sporulating phenotype was obtained by inactivating flbA in Aspergillus niger, which was accompanied by lysis, thinner cell walls, and an increased secretome complexity. Here, we further studied the role of the flb genes of A. niger. Strains ΔflbA, ΔflbB and ΔflbE showed increased biomass formation, while inactivation of flbA-D reduced, or even abolished, formation of conidia. Strain ΔflbA was more sensitive to H2O2, DTT, and the cell wall integrity stress compounds SDS and Congo Red (CR). Also, ΔflbC was more sensitive to SDS, while ΔflbB, ΔflbD, and ΔflbE were more sensitive to CR. On the other hand, inactivation of flbE increased resistance to H2O2. Enzyme secretion was impacted when the Δflb strains were grown on xylose. Strain ΔflbE showed reduced xylanase, cellulase and amylase secretion. On the other hand, amylase secretion at the periphery of the ΔflbA colony was reduced but not in its center, while secretion of this enzyme was increased in the center of the ΔflbB colony but not at its periphery. Inactivation of flbC and flbD also impacted zonal cellulase and amylase activity. Together, the Flb protein family of A. niger function in biomass formation, sporulation, stress response, and protein secretion.","PeriodicalId":501687,"journal":{"name":"Antonie van Leeuwenhoek","volume":"31 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140171014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Clostridium lamae sp. nov., a novel bacterium isolated from the fresh feces of alpaca 从羊驼新鲜粪便中分离出的一种新型细菌--羊驼梭状芽孢杆菌（Clostridium lamae sp.

Antonie van Leeuwenhoek Pub Date : 2024-02-17 DOI: 10.1007/s10482-024-01931-7

Xue Li, Ming Li, Weixiong Shi, Xia Li, Zhiguang Xiang, Lei Su

{"title":"Clostridium lamae sp. nov., a novel bacterium isolated from the fresh feces of alpaca","authors":"Xue Li, Ming Li, Weixiong Shi, Xia Li, Zhiguang Xiang, Lei Su","doi":"10.1007/s10482-024-01931-7","DOIUrl":"https://doi.org/10.1007/s10482-024-01931-7","url":null,"abstract":"A novel Gram-positive, anaerobic, nonspore-forming, rod-shaped bacterium, designated strain NGMCC 1.200840 T, was isolated from the alpacas fresh feces. The taxonomic position of the novel strain was determined using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences revealed strain NGMCC 1.200840 T was a member of the genus Clostridium and closely related to Clostridium tertium DSM 2485 T (98.16% sequence similarity). Between strains NGMCC 1.200840 T and C. tertium DSM 2485 T, the average nucleotide identity (ANI) and digital DNA˗DNA hybridization (dDDH) were 79.91% and 23.50%, respectively. Genomic DNA G + C content is 28.44 mol%. The strain can utilise D-glucose, D-mannitol, D-lactose, D-saccharose, D-maltose, D-xylose, L-arabinose, D-cellobiose, D-mannose, D-melezitose, D-raffinose, D-sorbitol, L-rhamnose, D-trehalose, D-galactose and Arbutin to produce acid. The optimal growth pH was 7, the temperature was 37 °C, and the salt concentration was 0–0.5% (w/v). The major cellular fatty acids (> 10%) included iso-C15:0, anteiso-C15:0 and iso-C17:0 3-OH. The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, three unidentified phospholipids and two unidentified aminolipids. Based on phenotypic, phylogenetic and chemotaxonomic characteristics, NGMCC 1.200840 T represents a novel species within the genus Clostridium, for which the named Clostridium lamae sp. nov. is proposed. The type strain is NGMCC 1.200840 T (= CGMCC 1.18014 T = JCM 35704 T).","PeriodicalId":501687,"journal":{"name":"Antonie van Leeuwenhoek","volume":"16 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139757413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Cell-free supernatants of probiotic consortia impede hyphal formation and disperse biofilms of vulvovaginal candidiasis causing Candida in an ex-vivo model 在体外模型中，益生菌联合体的无细胞上清液可阻碍外阴阴道念珠菌病致病念珠菌的菌丝形成并驱散其生物膜

Antonie van Leeuwenhoek Pub Date : 2024-02-17 DOI: 10.1007/s10482-024-01929-1

Sudaarsan Aruna Senthil Kumar, Dhesiga Krishnan, Sowndarya Jothipandiyan, Ramyadevi Durai, B. Narayanan Vedha Hari, Paramasivam Nithyanand

{"title":"Cell-free supernatants of probiotic consortia impede hyphal formation and disperse biofilms of vulvovaginal candidiasis causing Candida in an ex-vivo model","authors":"Sudaarsan Aruna Senthil Kumar, Dhesiga Krishnan, Sowndarya Jothipandiyan, Ramyadevi Durai, B. Narayanan Vedha Hari, Paramasivam Nithyanand","doi":"10.1007/s10482-024-01929-1","DOIUrl":"https://doi.org/10.1007/s10482-024-01929-1","url":null,"abstract":"Vulvovaginal candidiasis is the second most common vaginal infection caused by drug-resistant Candida species that affects about 70–75% of reproductive age group women across the globe. As current-day antifungal drugs are ineffective against the biofilms formed by the drug-resistant Candida strains, several natural compounds and antagonistic microbes are being explored as alternative antifungal agents. In the present study, we investigated the anti-biofilm activity of Cell-Free Supernatant (CFS) extracted from the commercially available probiotics VSL-3 against the biofilms of Candida species and also evaluated their efficacy in curbing the yeast-to-hyphal transition. Various methodologies like crystal violet staining and scanning electron microscopy were used to study the effect of CFS against the biofilms formed by the species. The ability of CFS to interfere with yeast to hyphal transition in Candida was studied by colony morphology assay and visually confirmed with phase contrast microscopy. The potential of the CFS of the probiotics was also evaluated using goat buccal tissue, a novel ex-vivo model that mimics the vaginal environment. Moreover, the supernatant extracted from VSL-3 had the ability to down-regulate the expression of virulence genes of Candida from the biofilm formed over the ex-vivo model. These results emphasize the anti-fungal and anti-infective properties of the CFS of VSL-3 against drug-resistant Candida strains causing vulvovaginal candidiasis.","PeriodicalId":501687,"journal":{"name":"Antonie van Leeuwenhoek","volume":"10 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139757542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Phocaeicola acetigenes sp. nov., producing acetic acid and iso-butyric acid, isolated faeces from a healthy human 从健康人粪便中分离出的产生醋酸和异丁酸的醋酸噬菌体新物种

Antonie van Leeuwenhoek Pub Date : 2024-02-01 DOI: 10.1007/s10482-024-01930-8

Hyo Eun Do, Young Bong Ha, Ji-Sun Kim, Min Kuk Suh, Han Sol Kim, Mi Kyung Eom, Ju Huck Lee, Seung-Hwan Park, Se Won Kang, Dong Ho Lee, Hyuk Yoon, Je Hee Lee, Jung-Sook Lee

{"title":"Phocaeicola acetigenes sp. nov., producing acetic acid and iso-butyric acid, isolated faeces from a healthy human","authors":"Hyo Eun Do, Young Bong Ha, Ji-Sun Kim, Min Kuk Suh, Han Sol Kim, Mi Kyung Eom, Ju Huck Lee, Seung-Hwan Park, Se Won Kang, Dong Ho Lee, Hyuk Yoon, Je Hee Lee, Jung-Sook Lee","doi":"10.1007/s10482-024-01930-8","DOIUrl":"https://doi.org/10.1007/s10482-024-01930-8","url":null,"abstract":"An obligately anaerobic, non-motile, Gram-stain-negative, and rod-shaped strain KGMB11183T was isolated from the feces of healthy Koreans. The growth of strain KGMB11183T occurred at 30–45 °C (optimum 37 °C), at pH 6–9 (optimum pH 7), and in the presence of 0–0.5% NaCl (optimum 0%). Strain KGMB11183T showed 16S rRNA gene sequence similarities of 95.4% and 94.2% to the closest recognized species, Phocaeicola plebeius M12T, and Phocaeicola faecicola AGMB03916T. Phylogenetic analysis showed that strain KGMB11183T is a member of the genus Phocaeiocla. The major end products of fermentation are acetic acid and isobutyric acid. The major cellular fatty acids (> 10%) of this isolate were C18:1 cis 9, anteiso-C15:0, and summed feature 11 (iso-C17:0 3-OH and/or C18:2 DMA). The assembled draft genome sequences of strain KGMB11183T consisted of 3,215,271 bp with a DNA G + C content of 41.4%. According to genomic analysis, strain KGMB11183T has a number of genes that produce acetic acid. The genome of strain KGMB11183T encoded the starch utilization system (Sus) operon, SusCDEF suggesting that strain uses many complex polysaccharides that cannot be digested by humans. Based on the physiological, chemotaxonomic, phenotypic, and phylogenetic data, strain KGMB11183T is regarded a novel species of the genus Phocaeicola. The type strain is KGMB11183T (= KCTC 25284T = JCM 35696T).","PeriodicalId":501687,"journal":{"name":"Antonie van Leeuwenhoek","volume":"299 2 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139667383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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