Alejandra de Jesús Chavarín-Meza, Bruno Gómez-Gil, Adrián González-Castillo
{"title":"Ponticus 支系的系统进化分析：从斑点玫瑰鲷（Lutjanus guttatus）中分离出的菌株","authors":"Alejandra de Jesús Chavarín-Meza, Bruno Gómez-Gil, Adrián González-Castillo","doi":"10.1007/s10482-024-01959-9","DOIUrl":null,"url":null,"abstract":"<p>The family Vibrionaceae is classified into many clades based on their phylogenetic relationships. The Ponticus clade is one of its clades and consists of four species, <i>Vibrio panuliri</i>, <i>V. ponticus</i>, <i>V. rhodolitus</i>, and <i>V. taketomensis</i>. Two strains, CAIM 703 and CAIM 1902, were isolated from the diseased spotted rose snapper external lesion (<i>Lutjanus guttatus</i>), they were analyzed to determine their taxonomic position, a phylogenetic analysis was performed based on the 16S rRNA sequences proved that the two strains are members of the genus <i>Vibrio</i> and they belong to the Ponticus clade. Then, a phylogenomic analysis was performed with four type strains and four reference strains isolated from marine organisms and aquatic environments. Multilocus Sequence Analysis (MLSA) of 139 single-copy genes showed that CAIM 703 and CAIM 1902 belong to <i>V. panuliri</i>. The 16S rRNA sequence similarity value between CAIM 703 and CAIM 1902 was 99.61%. The Ponticus clade species showed Average Nucleotide Identity (ANI) values between 78 to 80% against the two strains for ANIb, except <i>V. panuliri</i> LBS2<sup>T</sup> (99% and 100% similarity). Finally, this analysis represents the first phylogenomic analysis of the Ponticus clade where <i>V. panuliri</i> strains are reported from Mexico.</p>","PeriodicalId":501687,"journal":{"name":"Antonie van Leeuwenhoek","volume":"151 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2024-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Phylogenomic analysis of the Ponticus clade: strains isolated from the spotted rose snapper (Lutjanus guttatus)\",\"authors\":\"Alejandra de Jesús Chavarín-Meza, Bruno Gómez-Gil, Adrián González-Castillo\",\"doi\":\"10.1007/s10482-024-01959-9\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>The family Vibrionaceae is classified into many clades based on their phylogenetic relationships. The Ponticus clade is one of its clades and consists of four species, <i>Vibrio panuliri</i>, <i>V. ponticus</i>, <i>V. rhodolitus</i>, and <i>V. taketomensis</i>. Two strains, CAIM 703 and CAIM 1902, were isolated from the diseased spotted rose snapper external lesion (<i>Lutjanus guttatus</i>), they were analyzed to determine their taxonomic position, a phylogenetic analysis was performed based on the 16S rRNA sequences proved that the two strains are members of the genus <i>Vibrio</i> and they belong to the Ponticus clade. Then, a phylogenomic analysis was performed with four type strains and four reference strains isolated from marine organisms and aquatic environments. Multilocus Sequence Analysis (MLSA) of 139 single-copy genes showed that CAIM 703 and CAIM 1902 belong to <i>V. panuliri</i>. The 16S rRNA sequence similarity value between CAIM 703 and CAIM 1902 was 99.61%. The Ponticus clade species showed Average Nucleotide Identity (ANI) values between 78 to 80% against the two strains for ANIb, except <i>V. panuliri</i> LBS2<sup>T</sup> (99% and 100% similarity). Finally, this analysis represents the first phylogenomic analysis of the Ponticus clade where <i>V. panuliri</i> strains are reported from Mexico.</p>\",\"PeriodicalId\":501687,\"journal\":{\"name\":\"Antonie van Leeuwenhoek\",\"volume\":\"151 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-03-20\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Antonie van Leeuwenhoek\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1007/s10482-024-01959-9\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Antonie van Leeuwenhoek","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1007/s10482-024-01959-9","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Phylogenomic analysis of the Ponticus clade: strains isolated from the spotted rose snapper (Lutjanus guttatus)
The family Vibrionaceae is classified into many clades based on their phylogenetic relationships. The Ponticus clade is one of its clades and consists of four species, Vibrio panuliri, V. ponticus, V. rhodolitus, and V. taketomensis. Two strains, CAIM 703 and CAIM 1902, were isolated from the diseased spotted rose snapper external lesion (Lutjanus guttatus), they were analyzed to determine their taxonomic position, a phylogenetic analysis was performed based on the 16S rRNA sequences proved that the two strains are members of the genus Vibrio and they belong to the Ponticus clade. Then, a phylogenomic analysis was performed with four type strains and four reference strains isolated from marine organisms and aquatic environments. Multilocus Sequence Analysis (MLSA) of 139 single-copy genes showed that CAIM 703 and CAIM 1902 belong to V. panuliri. The 16S rRNA sequence similarity value between CAIM 703 and CAIM 1902 was 99.61%. The Ponticus clade species showed Average Nucleotide Identity (ANI) values between 78 to 80% against the two strains for ANIb, except V. panuliri LBS2T (99% and 100% similarity). Finally, this analysis represents the first phylogenomic analysis of the Ponticus clade where V. panuliri strains are reported from Mexico.
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