bioRxiv - Plant Biology最新文献

Directional Cell-to-cell Transport in Plant Roots 植物根部细胞间的定向运输

bioRxiv - Plant Biology Pub Date : 2024-09-19 DOI: 10.1101/2024.09.16.613234

Lea Jacquier, Celeste Fiorenza, Kevin Robe, Jian-Pu Han, Fabienne Cleard, Christelle Fuchs, Priya Ramakrishna, Sylvain Loubery, Linnka Lefebvre-Legendre, Marie Barberon

{"title":"Directional Cell-to-cell Transport in Plant Roots","authors":"Lea Jacquier, Celeste Fiorenza, Kevin Robe, Jian-Pu Han, Fabienne Cleard, Christelle Fuchs, Priya Ramakrishna, Sylvain Loubery, Linnka Lefebvre-Legendre, Marie Barberon","doi":"10.1101/2024.09.16.613234","DOIUrl":"https://doi.org/10.1101/2024.09.16.613234","url":null,"abstract":"Cell-to-cell communication is critical for multicellular organisms. In plants, plasmodesmata - cytoplasmic channels - enable molecular transport between adjacent cells. In roots, this transport is predicted to be essential in nutrient acquisition and delivery to the vasculature. We demonstrate that plasmodesmatal transport persists in differentiated roots, despite apoplastic barriers such as Casparian strips and suberin lamellae in the endodermis, suggesting plasmodesmata as the sole pathway for water and nutrient flow at this stage. We also reveal a developmental switch in plasmodesmata function resulting in an unidirectional transport in differentiated roots. A genetic screen identified mutations that disrupt this directionality, leading to bidirectional transport. These mutations correlate with larger plasmodesmatal apertures, linked to defects in pectin composition and cell wall organization. This discovery underscores the role of plasmodesmatal aperture regulation and pectin in controlling directional transport. Our findings provide insights into plasmodesmata function and their regulation in roots.","PeriodicalId":501341,"journal":{"name":"bioRxiv - Plant Biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142268366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrated Transcriptome and Metabolome Analyses Reveal the Mechanism Regulating Bulbil Initiation and Development in Cystopteris chinensis 转录组和代谢组的综合分析揭示了鞘翅目蝉科昆虫鳞茎萌发和发育的调控机制

bioRxiv - Plant Biology Pub Date : 2024-09-19 DOI: 10.1101/2024.09.18.613657

An Yu, Xiaohong Chen, Wenkai Xi, Xia Zhao, Yazhu Wang, Zhihong Gong, Xiaofeng Zhou

{"title":"Integrated Transcriptome and Metabolome Analyses Reveal the Mechanism Regulating Bulbil Initiation and Development in Cystopteris chinensis","authors":"An Yu, Xiaohong Chen, Wenkai Xi, Xia Zhao, Yazhu Wang, Zhihong Gong, Xiaofeng Zhou","doi":"10.1101/2024.09.18.613657","DOIUrl":"https://doi.org/10.1101/2024.09.18.613657","url":null,"abstract":"Cystopteris chinensis is an endangered fern endemic to China, which only has a small wild population due to its poor reproductive ability. However, we recently found that it can produce bulbils on its pinnule to generate new plants but the molecular mechanism underlying this unique phenomenon remained unknown. In this study, we have identified four distinct stages in the initiation and development of bulbils based on morphological and anatomical observation. We performed transcriptome and metabolome analyses on the collected samples at each stage. Through KEGG enrichment analysis, it was found that the phytohormone signal transduction, starch and sucrose metabolism, phenylpropanoid biosynthesis, and flavonoid biosynthesis pathways play a significant role in regulating bulbil initiation and development. Specifically, the involvement of three phytohormones and sugar substances was identified in the process of bulbil initiation. Our study provides the first detailed observation of the bulbils in C. chinensis and explains their initiation and development at the molecular level. However, more in-depth studies are needed to discover the functions of key genes controlling the formation of bulbils to conserve the endangered C. chinensis population.","PeriodicalId":501341,"journal":{"name":"bioRxiv - Plant Biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142268363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stigma longevity is not a major limiting factor in hybrid wheat seed production 柱头寿命不是杂交小麦种子生产的主要限制因素

bioRxiv - Plant Biology Pub Date : 2024-09-18 DOI: 10.1101/2024.09.13.612789

Marina Millan-Blanquez, James Simmonds, Nicholas Bird, Yann Manes, Cristobal Uauy, Scott Andrew Boden

{"title":"Stigma longevity is not a major limiting factor in hybrid wheat seed production","authors":"Marina Millan-Blanquez, James Simmonds, Nicholas Bird, Yann Manes, Cristobal Uauy, Scott Andrew Boden","doi":"10.1101/2024.09.13.612789","DOIUrl":"https://doi.org/10.1101/2024.09.13.612789","url":null,"abstract":"Hybrids offer a promising approach to improve crop performance because the progeny are often superior to their parent lines and they outyield inbred varieties. A major challenge in producing hybrid progeny in wheat, however, lies in the low outcrossing rates of the maternal parent. This is often attributed to suboptimal synchronisation of male and female flowering as delayed pollination can result in reproductive failure due to female stigma deterioration. To test this accepted dogma, we examined the seed set capacity of six male sterile (MS) cultivars, each varying in the onset of stigma deterioration. To mimic a hybrid seed production scenario, MS cultivars were grown during two consecutive field seasons, and open pollination was allowed up to 15 days after flowering of the female parent using a blend of seven male fertile cultivars with varying flowering times. Detailed analysis of the temporal and spatial distribution of hybrid seed set along the spike across the six MS cultivars showed that grain production remained remarkably stable during the pollination window tested. These findings suggest sustained receptivity of stigma to pollen across all tested MS cultivars throughout the entire time course. We therefore conclude that stigma longevity does not represent a limiting factor in hybrid wheat seed production, and that breeding efforts should prioritise the study of other female traits, such as enhanced access to airborne pollen.","PeriodicalId":501341,"journal":{"name":"bioRxiv - Plant Biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142268365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bundle sheath cell-dependent chloroplast movement in mesophyll cells of C4 plants analyzed using live leaf-section imaging 利用活体叶片切片成像技术分析 C4 植物叶肉细胞中依赖束鞘细胞的叶绿体运动

bioRxiv - Plant Biology Pub Date : 2024-09-18 DOI: 10.1101/2024.09.13.612856

Yuta Kato, Takao Oi, Yoshikatsu Sato, Mitsutaka Taniguchi

{"title":"Bundle sheath cell-dependent chloroplast movement in mesophyll cells of C4 plants analyzed using live leaf-section imaging","authors":"Yuta Kato, Takao Oi, Yoshikatsu Sato, Mitsutaka Taniguchi","doi":"10.1101/2024.09.13.612856","DOIUrl":"https://doi.org/10.1101/2024.09.13.612856","url":null,"abstract":"In C4 plants, mesophyll (M) chloroplasts aggregate toward bundle sheath (BS) cells in response to environmental stress, which would contribute to C4 photosynthetic cycle adjustment between M and BS cells. However, it remains unclear whether mesophyll chloroplast movement is an intercellular response mediated by BS cells. One major challenge to resolving this is the difficulty in observing how M chloroplasts aggregate toward adjacent BS cells due to scattering and absorption of observation light in live-leaf tissues. We established a live leaf-section imaging technique that enables the long-term observation of sections of chemically unfixed leaf blades, with which we quantitatively analyzed M chloroplast movements. Another challenge in clarifying the contribution of BS cells to M chloroplast movement is the selective ablation of BS cells without impairing their function of M cells. To investigate the necessity of BS cells for M chloroplast movement, we developed a method to remove BS cells only based on differences in shape and size between M and BS cells. We also found that chloroplasts in M cells without adjacent BS cell contents did not show typical aggregative movement but showed a light-avoidance response. This indicates that the M chloroplast aggregative movement occurs during communication with BS cells.","PeriodicalId":501341,"journal":{"name":"bioRxiv - Plant Biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142268364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genotype by environment interactions in gene regulation underlie the response to soil drying in the model grass Brachypodium distachyon 模式草Brachypodium distachyon对土壤干燥的反应是基因调控中基因型与环境相互作用的结果

bioRxiv - Plant Biology Pub Date : 2024-09-18 DOI: 10.1101/2024.06.17.597683

Jie Yun, Angela C Burnett, Alistair Rogers, David L Des Marais

{"title":"Genotype by environment interactions in gene regulation underlie the response to soil drying in the model grass Brachypodium distachyon","authors":"Jie Yun, Angela C Burnett, Alistair Rogers, David L Des Marais","doi":"10.1101/2024.06.17.597683","DOIUrl":"https://doi.org/10.1101/2024.06.17.597683","url":null,"abstract":"Gene expression is a quantitative trait under the control of genetic and environmental factors and their interaction, so-called GxE. Understanding the mechanisms driving GxE is fundamental for ensuring stable crop performance across environments and for predicting the response of natural populations to climate change. Gene expression is regulated through complex molecular networks, yet the interactions between genotype and environment on genome-wide regulatory networks are rarely considered. In this study, we model genome-scale gene expression variation between two natural accessions of the model grass <em>Brachypodium distachyon</em> and their response to soil drying. We identified genotypic, environmental, and GxE responses in physiological, metabolic, and gene expression traits. We then identified gene regulation conservation and variation among conditions and genotypes, simplified as co-expression clusters in each combination of genotype and environmental treatment. Putative gene regulatory interactions are inferred as network edges with a graphical modelling approach, resulting in hypotheses about gene-gene interactions specific to -- or with higher affinity in -- one genotype, one treatment, or in one genotype under treatment. We further find that some gene-gene interactions are conserved across conditions such that differential expression of one gene is apparently transmitted to a target gene. These variably detected edges cluster together in co-expression modules, suggestive of different constraints or selection strength acting on specific pathways. These variable features of gene regulatory networks may represent candidates modulate environmental response via genome editing, and suggest possible targets of evolutionary change in gene regulatory networks associated with phenotypic plasticity.","PeriodicalId":501341,"journal":{"name":"bioRxiv - Plant Biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142268368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Implementation of Ribo-BiFC method to plant systems using a split mVenus approach 使用分割 mVenus 方法在植物系统中实施 Ribo-BiFC 方法

bioRxiv - Plant Biology Pub Date : 2024-09-17 DOI: 10.1101/2024.09.12.612679

Karel Raabe, Alena Náprstková, Janto Pieters, Elnura Torutaeva, Veronika Jirásková, Zahra Kahrizi, Christos Michailidis, David Honys

{"title":"Implementation of Ribo-BiFC method to plant systems using a split mVenus approach","authors":"Karel Raabe, Alena Náprstková, Janto Pieters, Elnura Torutaeva, Veronika Jirásková, Zahra Kahrizi, Christos Michailidis, David Honys","doi":"10.1101/2024.09.12.612679","DOIUrl":"https://doi.org/10.1101/2024.09.12.612679","url":null,"abstract":"Translation is a fundamental process for every living organism. In plants, the rate of translation is tightly modulated during development and in response to environmental cues. However, it is difficult to measure the actual translation state of the tissues in vivo. Here, we report the implementation of an in vivo translation marker based on bimolecular fluorescence complementation, the Ribo-BiFC. We combined method originally developed for fruit-fly with an improved low background split-mVenus BiFC system previously described in plants. We labelled Arabidopsis thaliana small subunit ribosomal protein (RPS) and large subunit ribosomal protein (RPL) with fragments of the mVenus fluorescent protein. Upon the assembly of the 80S ribosome, the mVenus fragments complemented and were detected by fluorescent microscopy. We show that these recombinant proteins are in close proximity in the tobacco epidermal cells, although the signal is reduced when compared to BiFC signal from known interactors. This Ribo-BiFC method system can be used in stable transgenic lines to enable visualisation of translational rate in plant tissues and could be used to study translation dynamics and its changes during plant development, under abiotic stress or in different genetic backgrounds.","PeriodicalId":501341,"journal":{"name":"bioRxiv - Plant Biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142268367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The H3K4me3 binding ALFIN-LIKE proteins recruit SWR1 for gene-body deposition of H2A.Z 与 H3K4me3 结合的 ALFIN-LIKE 蛋白招募 SWR1 进行 H2A.Z 的基因体沉积

bioRxiv - Plant Biology Pub Date : 2024-09-17 DOI: 10.1101/2024.09.12.612642

Linhao Xu, Yafei Wang, Xueying Li, Qin Hu, Vanda Adamkova, Junjie Xu, C. Jake Harris, Israel Ausin

引用次数: 0

Diversification and conservation of DNA binding specificities of SPL family of transcription factors SPL 转录因子家族 DNA 结合特异性的多样化和保护

bioRxiv - Plant Biology Pub Date : 2024-09-16 DOI: 10.1101/2024.09.13.612952

Miaomiao Li, Tao Yao, Mary Galli, Wanru Lin, Yilin Zhou, Jin-Gui Chen, Andrea Gallavotti, Shao-shan Carol Huang

引用次数: 0

The transcription factor ZmbZIP75 promotes both grain filling and kernel dehydration in maize 转录因子 ZmbZIP75 可促进玉米籽粒灌浆和籽粒脱水

bioRxiv - Plant Biology Pub Date : 2024-09-16 DOI: 10.1101/2024.09.11.612493

Tiandan Long, Yayun Wang, Jin Yang, Zhou Liu, Changqing Mao, Yufeng Hu, Junjie Zhang, Hanmei Liu, Yinghong Liu, Xiujun Fan, Lei Gao, Huanhuan Huang, Ying Xie, Daqiu Zhao, Yubi Huang, Yangping Li

{"title":"The transcription factor ZmbZIP75 promotes both grain filling and kernel dehydration in maize","authors":"Tiandan Long, Yayun Wang, Jin Yang, Zhou Liu, Changqing Mao, Yufeng Hu, Junjie Zhang, Hanmei Liu, Yinghong Liu, Xiujun Fan, Lei Gao, Huanhuan Huang, Ying Xie, Daqiu Zhao, Yubi Huang, Yangping Li","doi":"10.1101/2024.09.11.612493","DOIUrl":"https://doi.org/10.1101/2024.09.11.612493","url":null,"abstract":"Selecting both high-yield and low-kernel-moisture varieties is essential for modern maize production, but relevant breeding efforts are hindered by a lack of valuable regulatory genes. Here, we demonstrate that the transcription factor (TF) basic leucine zipper 75 (ZmbZIP75) promotes grain yield and reduces kernel moisture in maize. Knockout of ZmbZIP75 results in defective grain filling and kernel dehydration, whereas ZmbZIP75 overexpression confers increased grain yield per plant and decreased kernel moisture without altering plant architecture. Mechanistically, during the grain filling stage, ZmbZIP75 is transcriptionally induced by maternal-derived basal abscisic acid (ABA) and directly activates multiple core starch synthesis-related genes and key TFs, thereby promoting grain filling and final yield. In the late stage of kernel development, high concentrations of zygotic ABA enhance ZmbZIP75 phosphorylation through SnRK2.10. The phosphorylated ZmbZIP75 subsequently transactivates and interacts with TF VP1 to synergistically promote kernel dehydration. This study thus highlights the potential of ZmbZIP75 for engineering both high-yield and low-kernel-moisture varieties to meet the demands of high-efficient maize production.","PeriodicalId":501341,"journal":{"name":"bioRxiv - Plant Biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142268370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Natural variation in root exudate composition in the genetically structured Arabidopsis thaliana in the Iberian Peninsula 伊比利亚半岛基因结构拟南芥根部渗出物成分的自然变化

bioRxiv - Plant Biology Pub Date : 2024-09-16 DOI: 10.1101/2024.09.11.612578

Harihar Jaishree Subrahmaniam, F. Xavier Pico, Thomas Bataillon, Camilla Lind Salomonsen, Marianne Glasius, Bodil K. Ehlers

{"title":"Natural variation in root exudate composition in the genetically structured Arabidopsis thaliana in the Iberian Peninsula","authors":"Harihar Jaishree Subrahmaniam, F. Xavier Pico, Thomas Bataillon, Camilla Lind Salomonsen, Marianne Glasius, Bodil K. Ehlers","doi":"10.1101/2024.09.11.612578","DOIUrl":"https://doi.org/10.1101/2024.09.11.612578","url":null,"abstract":"Plant root exudates are involved in nutrient acquisition, microbial partnerships, and inter-organism signaling. Yet, little is known about the genetic and environmental drivers of root exudate variation at large geographical scales, which may help understand evolutionary trajectories of plants in heterogeneous environments. We quantified natural variation in chemical composition of Arabidopsis thaliana root exudates in 105 Iberian accessions. We identified up to 373 putative compounds using ultra high performance liquid chromatography coupled with mass spectrometry. We estimated broad-sense heritability of compounds and conducted a genome-wide association (GWA) study. We associated variation in root exudates to variation in geographic, environmental, life history, and genetic attributes of Iberian accessions. Only 25 of 373 compounds exhibited broad-sense heritability values significantly different from zero. GWA analysis identified polymorphisms associated to 12 root exudate compounds and 26 known genes involved in metabolism, defense, signaling, and nutrient transport. The genetic structure influenced root exudate composition involving terpenoids. We detected five terpenoids related to plant defense significantly varying in mean abundances in two genetic clusters. Our study provides first insights into the extent of root exudate natural variation at a regional scale depicting a diversified evolutionary trajectory among A. thaliana genetic clusters chiefly mediated by terpenoid composition.","PeriodicalId":501341,"journal":{"name":"bioRxiv - Plant Biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142268369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0