Methods in Microbiology最新文献_第2页

Current molecular diagnostics assays for SARS-CoV-2 and emerging variants. 目前针对SARS-CoV-2和新出现变体的分子诊断分析

4区生物学

Methods in Microbiology Pub Date : 2022-01-01 Epub Date: 2021-12-09 DOI: 10.1016/bs.mim.2021.10.003

Jonathan M Banks, Kristelle Capistrano, Pari Thakkar, Hemangi Ranade, Vaidik Soni, Manali Datta, Afsar R Naqvi

{"title":"Current molecular diagnostics assays for SARS-CoV-2 and emerging variants.","authors":"Jonathan M Banks, Kristelle Capistrano, Pari Thakkar, Hemangi Ranade, Vaidik Soni, Manali Datta, Afsar R Naqvi","doi":"10.1016/bs.mim.2021.10.003","DOIUrl":"10.1016/bs.mim.2021.10.003","url":null,"abstract":"Since the SARS-CoV-2 virus triggered the beginning of the COVID-19 pandemic, scientists, government officials, and healthcare professionals around the world recognized the need for accessible, affordable, and accurate testing to predict and contain the spread of COVID-19. In the months that followed, research teams designed, tested, and rolled out hundreds of diagnostic assays, each with different sampling methods, diagnostic technologies, and sensitivity levels. However, the contagious virus continued to spread; SARS-CoV-2 travelled through airborne particles and spread rapidly, despite the widening use of diagnostic assays. As the pandemic continued, hundreds of millions of people contracted COVID-19 and millions died worldwide. With so many infections, SARS-CoV-2 received many opportunities to replicate and mutate, and from these mutations emerged more contagious, deadly, and difficult-to-diagnose viral mutants. Each change to the viral genome presented potential added challenges to containing the virus, and as such, researchers have continued developing and improving testing methods to keep up with COVID-19. In this chapter, we examine several SARS-CoV-2 variants that have emerged during the pandemic. Additionally, we discuss a few major COVID-19 diagnostic technique categories, including those involving real-time PCR, serology, CRISPR, and electronic biosensors. Finally, we address SARS-CoV-2 variants and diagnostic assays in the age of COVID-19 vaccines.","PeriodicalId":49823,"journal":{"name":"Methods in Microbiology","volume":"50 1","pages":"83-121"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8655725/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49018278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Covid-19: Biomedical Perspectives Covid-19:生物医学视角

4区生物学

Methods in Microbiology Pub Date : 2022-01-01 DOI: 10.1016/s0580-9517(22)x0002-0

引用次数: 2

The emergence of SARS-CoV-2 variants of concern in Australia by haplotype coalescence reveals a continental link to COVID-19 seasonality. 澳大利亚通过单倍型合并出现的SARS-CoV-2变异毒株揭示了与新冠肺炎季节性的大陆联系

4区生物学

Methods in Microbiology Pub Date : 2022-01-01 Epub Date: 2022-05-17 DOI: 10.1016/bs.mim.2022.03.003

Tre Tomaszewski, Volker Gurtler, Kelsey Caetano-Anollés, Gustavo Caetano-Anollés

{"title":"The emergence of SARS-CoV-2 variants of concern in Australia by haplotype coalescence reveals a continental link to COVID-19 seasonality.","authors":"Tre Tomaszewski, Volker Gurtler, Kelsey Caetano-Anollés, Gustavo Caetano-Anollés","doi":"10.1016/bs.mim.2022.03.003","DOIUrl":"10.1016/bs.mim.2022.03.003","url":null,"abstract":"SARS-CoV-2 continues to evolve, even after implementation of public-wide vaccination, as can be observed by an increasing number of mutations over time. Compared to responses by the United States and European countries, the disease mitigation strategies employed by the Australian government have been swift and effective. This provides a unique opportunity to study the emergence of variants of concern (VOCs) at many latitude levels in a country that has been able to control infection for the majority of the pandemic. In the present study, we explored the occurrence and accumulation of major mutations typical of VOCs in different regions of Australia and the effects that latitude has on the establishment of VOC-induced disease. We also studied the constellation of mutations characteristic of VOCs to determine if the mutation sets acted as haplotypes. Our goal was to explore processes behind the emergence of VOCs as the viral disease progresses towards becoming endemic. Most reported COVID-19 cases were in largest cities located within a -30°S to - 50°S latitude corridor previously identified to be associated with seasonal behavior. Accumulation plots of individual amino acid variants of major VOCs showed that the first major haplotypes reported worldwide were also present in Australia. A classification of accumulation plots revealed the existence of 18 additional haplotypes associated with VOCs alpha, delta and omicron. Core mutant constellations for these VOCs and curve overlaps for variants in each set of haplotypes demonstrated significant decoupling patterns, suggesting processes of emergence. Finally, construction of a \"haplotype network\" that describes the viral population landscape of Australia throughout the COVID-19 pandemic revealed significant and unanticipated seasonal patterns of emergence and diversification. These results provide a unique window into our evolutionary understanding of a human pathogen of great significance. They may guide future research into mitigation and prediction strategies for future VOCs.","PeriodicalId":49823,"journal":{"name":"Methods in Microbiology","volume":"50 1","pages":"233-268"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9110064/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42054499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular approaches for detection of pebrine disease in sericulture 蚕桑微囊病的分子检测方法

4区生物学

Methods in Microbiology Pub Date : 2021-01-01 DOI: 10.1016/bs.mim.2021.04.004

N. Chandrakanth, Pooja Makwana, L. Satish, M. Rabha, V. Sivaprasad

引用次数: 1

Recent trends in tasar silkworm Antheraea mylitta Drury disease management 柞蚕病防治的最新趋势

4区生物学

Methods in Microbiology Pub Date : 2021-01-01 DOI: 10.1016/bs.mim.2021.05.001

G. P. Singh, M. M. Baig, C.M. Bajpayi

引用次数: 0

Immune mechanism in silkworm Bombyx mori L. 家蚕的免疫机制。