Science in China. Series C, Life Sciences / Chinese Academy of Sciences最新文献

{"title":"Configural processing of different topologically structured figures: an ERP study.","authors":"JunJun Zhang,&nbsp;WeiNa Zhu,&nbsp;XiaoJun Ding,&nbsp;ChangLe Zhou,&nbsp;XinTian Hu,&nbsp;YuanYe Ma","doi":"10.1007/s11427-009-0150-0","DOIUrl":"https://doi.org/10.1007/s11427-009-0150-0","url":null,"abstract":"<p><p>According to Chen's theory, topological differences are perceived faster than feature differences in early visual perception. We hypothesized that topological perception is caused by the sensitivity in discriminating figures with and without \"holes\". An ERP experiment was conducted utilizing a passive paradigm to investigate the differences in perceiving figures with \"hole\" and with \"no-hole\". The results showed differences in N170 components between figures with \"holes\" and with \"no-holes\". The inversion of the \"hole\" could influence the latency of N170, but the inversion of the \"no-hole\" could not, which indicated that global features are processed first in the \"hole\" perception whilst local features are given priority to the \"no-hole\" perception. This result was similar to studies concerning face and non-face objects, suggesting a configural processing of the \"hole\".</p>","PeriodicalId":49127,"journal":{"name":"Science in China. Series C, Life Sciences / Chinese Academy of Sciences","volume":"52 12","pages":"1198-204"},"PeriodicalIF":0.0,"publicationDate":"2009-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s11427-009-0150-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28599803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Circulating miRNA and cancer diagnosis.","authors":"Yi Tie,&nbsp;BinDong Liu,&nbsp;HanJiang Fu,&nbsp;XiaoFei Zheng","doi":"10.1007/s11427-009-0158-5","DOIUrl":"https://doi.org/10.1007/s11427-009-0158-5","url":null,"abstract":"<p><p>miRNAs are a class of small RNA molecules with regulatory function, and play an important role in tumor development and progression. It has been demonstrated that tumor-derived miRNAs exist in the circulating nucleic acids of cancer patients. This phenomenon implies that detection of the circulating miRNA may be an effective method for non-invasive diagnosis of cancer. In this review, we summarize the applications of the circulating miRNA as biomarkers in cancer diagnosis, as well as the latest research progress in this area.</p>","PeriodicalId":49127,"journal":{"name":"Science in China. Series C, Life Sciences / Chinese Academy of Sciences","volume":"52 12","pages":"1117-22"},"PeriodicalIF":0.0,"publicationDate":"2009-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s11427-009-0158-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28599916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Global transcriptional profiles of Trichophyton rubrum in response to Flucytosine.","authors":"Rong Zhao,&nbsp;Wen Bin,&nbsp;YouJiang Diao,&nbsp;Jian Yang,&nbsp;Tao Liu,&nbsp;JunPing Peng,&nbsp;Qi Jin","doi":"10.1007/s11427-009-0153-x","DOIUrl":"https://doi.org/10.1007/s11427-009-0153-x","url":null,"abstract":"<p><p>Trichophyton rubrum (T.rubrum) is one of the most common human fungal pathogens that cause chronic infections of the skin and nails. To identify antifungal responsive genes, cDNA microarray analysis was performed for T. rubrum to reveal global transcriptional profiles of drug-specific responses to 5-Flucytosine (5-FC). cDNA microarray was constructed from the T. rubrum expressed sequence tag (ESTs) database, the minimum inhibitory concentration (MIC) of 5-FC was determined, and microarray hybridization and data analysis were applied. The expression pattern of 7 genes observed by microarray was confirmed by the quantitative real-time reverser transcription polymerase chain reaction (RT-PCR). Data analysis indicated that a total of 474 genes were found differentially expressed, 196 showed an increase in expression and 278 showed a decrease in expression. Marked down-regulation of genes involved in nucleotide metabolism (such as CDC21), transcription (such as E2F1), and RNA processing (such as SGN1, RIM4 and NOP1) was observed. Other genes involved in signal transduction, chaperones, inorganic ion transport, secondary metabolite biosynthesis, amino acid transport, lipid transport and potential drug resistance mechanism were also affected by 5-FC. Quantitative real-time RT-PCR of the selected genes confirmed the reliability of the microarray results. This is the first analysis of transcriptional profiles in response to 5-FC for T. rubrum. The findings may be valuable for the identification of genes involved in mechanisms of action and mechanisms of antifungal drug resistance of 5-FC.</p>","PeriodicalId":49127,"journal":{"name":"Science in China. Series C, Life Sciences / Chinese Academy of Sciences","volume":"52 12","pages":"1173-85"},"PeriodicalIF":0.0,"publicationDate":"2009-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s11427-009-0153-x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28601380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"microRNA-181b通过靶定 MLK2 调节白血病细胞HL-60的增殖","authors":"米延 陈宏陈群方明, Chen Qun Fang Meng Mi Yan Chen Hong","doi":"10.1360/ZC2009-39-11-1034","DOIUrl":"https://doi.org/10.1360/ZC2009-39-11-1034","url":null,"abstract":"microRNA在细胞的转移、凋亡、分化等生命过程中发挥着重要的作用. miR-181b在急髓性白血病组织中高表达, 通过靶定MLK2调节急髓性白血病细胞的增殖. 本结果提示, miR-181b在急髓性白血病的生物学过程发挥着重要的作用, 同时为急髓性白血病的治疗提供了新的解决方案.","PeriodicalId":49127,"journal":{"name":"Science in China. Series C, Life Sciences / Chinese Academy of Sciences","volume":"18 1","pages":"1034-1040"},"PeriodicalIF":0.0,"publicationDate":"2009-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77489601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"体外模拟脑缺血再灌注条件下BV-2细胞TLR4 信号途径对TLR2表达的影响","authors":"孙鹏 张清 韩继媛 田元 张景辉, Zhang Qing Han Ji-Yuan Tian Yuan Zhang Jing-Hui Sun Feng","doi":"10.1360/ZC2009-39-11-1013","DOIUrl":"https://doi.org/10.1360/ZC2009-39-11-1013","url":null,"abstract":"研究证实, TLR4和TLR2有可能作为重要炎性受体介导脑缺血再灌注炎性损伤. 然而目前还不清楚在此过程中TLR2和TLR4受体之间是否存在着交叉对话可能. 本研究首先利用针对TLR4基因的RNA干扰技术阻断体外模拟脑I/R条件下BV-2细胞TLR4信号传导途径, 观察此时TLR2受体表达变化, 初步探讨TLR4信号途径对TLR2表达的影响. 然后利用NF-κB抑制剂PDTC阻断NF-κB活性来观察体外模拟脑I/R条件下BV-2细胞TLR2和TLR4受体表达变化, 进一步阐明NF-κB在TLR2和TLR4交叉对话中的作用. 结果表明: (1) 体外模拟脑I/R条件下阻断BV-2细胞TLR4信号传导途径可以明显抑制TLR2和NF-κB表达的上调; (2) PDTC预处理后在体外模拟脑I/R条件下BV-2细胞 TLR2和TLR4的表达均下调. 结果提示, 脑I/R损伤中TLR4受体激活后有可能通过NF-κB的介导, 进一步影响TLR2的表达, 从而导致炎症反应的链式放大, 两者协同加重了脑损伤的过程.","PeriodicalId":49127,"journal":{"name":"Science in China. Series C, Life Sciences / Chinese Academy of Sciences","volume":"10 1","pages":"1013-1018"},"PeriodicalIF":0.0,"publicationDate":"2009-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89256111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Proteomic analysis of mitochondria reveals a metabolic switch from fatty acid oxidation to glycolysis in the failing heart.","authors":"Jun Wang,&nbsp;Ling Bai,&nbsp;Jing Li,&nbsp;ChaoFeng Sun,&nbsp;Jin Zhao,&nbsp;ChangCong Cui,&nbsp;Ke Han,&nbsp;Yu Liu,&nbsp;XiaoZhen Zhuo,&nbsp;TingZhong Wang,&nbsp;Ping Liu,&nbsp;FenLing Fan,&nbsp;YouFei Guan,&nbsp;AiQun Ma","doi":"10.1007/s11427-009-0140-2","DOIUrl":"https://doi.org/10.1007/s11427-009-0140-2","url":null,"abstract":"<p><p>This work characterizes the mitochondrial proteomic profile in the failing heart and elucidates the molecular basis of mitochondria in heart failure. Heart failure was induced in rats by myocardial infarction, and mitochondria were isolated from hearts by differential centrifugation. Using two-dimensional gel electrophoresis and matrix-assisted laser desorption/ionization-time of flight mass spectrometry, a system biology approach was employed to investigate differences in mitochondrial proteins between normal and failing hearts. Mass spectrometry identified 27 proteins differentially expressed that involved in energy metabolism. Among those, the up-regulated proteins included tricarboxylic acid cycle enzymes and pyruvate dehydrogenase complex subunits while the down-regulated proteins were involved in fatty acid oxidation and the OXPHOS complex. These results suggest a substantial metabolic switch from free fatty acid oxidation to glycolysis in heart failure and provide molecular evidence for alterations in the structural and functional parameters of mitochondria that may contribute to cardiac dysfunction during ischemic injury.</p>","PeriodicalId":49127,"journal":{"name":"Science in China. Series C, Life Sciences / Chinese Academy of Sciences","volume":"52 11","pages":"1003-10"},"PeriodicalIF":0.0,"publicationDate":"2009-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s11427-009-0140-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28527489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect and mechanism of acute graft versus host disease on early diffuse murine lung injury following allogeneic stem cell transplantation.","authors":"Juan Ning,&nbsp;Qi Fa Liu,&nbsp;Xiao Dan Luo,&nbsp;Zhi Ping Fan,&nbsp;Yu Zhang","doi":"10.1007/s11427-009-0139-8","DOIUrl":"https://doi.org/10.1007/s11427-009-0139-8","url":null,"abstract":"<p><p>To explore the effect and pathogenssis of acute graft-versus-host disease (aGVHD) on early diffuse lung injury in allogeneic hematopoietic stem cell transplantation (allo-HSCT), we established an aGVHD model of C(57)BL/6-->BALB/c mice. Chest computed tomography (CT) scans, histopathology and the levels of cytokines including tumor necrosis factor alpha (TNFalpha) and Interferon (IFNgamma) in lungs were dynamically detected in recipient mice after transplantation. The incidence of aGVHD was respectively 0%, 0% and 100% in simple irradiation group (A), syngeneic transplant group(B) and allogeneic transplant group (C). Chest CT scans of recipient mice were normal in 3 groups on days +3 and +7 after transplantation. CT showed that two of ten mice had bilateral lung diffuse infiltrate on day +12 (on the brink of death) in group A and 6 of 10 mice had bilateral lung diffuse infiltrate on day +14 (3 d after aGVHD occurring) in group C, and were normal on days +12 and +14 in group B after transplantation. Histopathology of lungs in the 3 groups was similar, consisting of minor interstitial pneumonitis on day +3. Group A showed edema, hyperplasia of epithelial cells and widened alveolar interval on day +7, and epithelial cell necrosis, lymphocyte infiltration, hemorrhage, protein leakage, and local consolidation on day +12. The histopathology of group B showed slight edema of epithelial cells on +7 day, which were slighter than that on day +3, and virtually normal on day +14. The histopathology in group C was characterized by the significant expansion and congestion of capillaries, and lymphocyte infiltration on day +7, the acute pneumonitis was present involving tissue edema, lymphocyte and macrophage infiltration, protein leakage and perivascular inflammation on day +14. In group A, the levels of TNFalpha were lower on day +7 than on day +3. In group B, the levels of TNFalpha attained a peak on day +3, which decreased on days +7 and +14. In group C, the levels of TNFalpha were highest on day +7 and there was a significant difference between those on days +7 and +14 (P=0.816). In group A, the levels of IFNgamma on day +7 were higher than on day +3. In group B, the levels of IFNgamma increased progressively, but the comparison of IFNgamma levels in different times had no statistical significance (P=0.521, 0.118, 0.340). In group C, the levels of IFNgamma attained a peak by day +7 and decreased on day +14. aGVHD is the main cause of early non-infectious lung injury. T lymphocytes and TNFalpha are possibly implicated in the pathogenesis of acute GVHD-induced lung injury. The decreased levels of IFNgamma in lung tissues following transplantation might be associated with pulmonary fibrosis in late non-infectious pulmonary complications.</p>","PeriodicalId":49127,"journal":{"name":"Science in China. Series C, Life Sciences / Chinese Academy of Sciences","volume":"52 11","pages":"1016-22"},"PeriodicalIF":0.0,"publicationDate":"2009-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s11427-009-0139-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28527491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modeling and simulation of ion channels and action potentials in taste receptor cells.","authors":"PeiHua Chen,&nbsp;Xiao-dong Liu,&nbsp;Wei Zhang,&nbsp;Jun Zhou,&nbsp;Ping Wang,&nbsp;Wei Yang,&nbsp;JianHong Luo","doi":"10.1007/s11427-009-0138-9","DOIUrl":"https://doi.org/10.1007/s11427-009-0138-9","url":null,"abstract":"<p><p>Based on patch clamp data on the ionic currents of rat taste receptor cells, a mathematical model of mammalian taste receptor cells was constructed to simulate the action potentials of taste receptor cells and their corresponding ionic components, including voltage-gated Na(+) currents and outward delayed rectifier K(+) currents. Our simulations reproduced the action potentials of taste receptor cells in response to electrical stimuli or sour tastants. The kinetics of ion channels and their roles in action potentials of taste receptor cells were also analyzed. Our prototype model of single taste receptor cell and simulation results presented in this paper provide the basis for the further study of taste information processing in the gustatory system.</p>","PeriodicalId":49127,"journal":{"name":"Science in China. Series C, Life Sciences / Chinese Academy of Sciences","volume":"52 11","pages":"1036-47"},"PeriodicalIF":0.0,"publicationDate":"2009-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s11427-009-0138-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28527494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The expression profile of genes in rice roots under low phosphorus stress.","authors":"LiHua Li,&nbsp;XuHua Qiu,&nbsp;XiangHua Li,&nbsp;ShiPing Wang,&nbsp;XingMing Lian","doi":"10.1007/s11427-009-0137-x","DOIUrl":"https://doi.org/10.1007/s11427-009-0137-x","url":null,"abstract":"<p><p>Phosphorus (P) is one of the most essential macronutrients required for plant growth. Although it is abundant in soil, P is often the limiting nutrient for crop yield potential because of the low concentration of soluble P that plants can absorb directly. The gene expression profile was investigated in rice roots at 6, 24 and 72 h under low P stress and compared with a control (normal P) profile, using a DNA chip of 60000 oligos (70 mer) that represented all putative genes of the rice genome. A total of 795 differentially expressed genes were identified in response to phosphate (Pi) starvation in at least one of the treatments. Based on the analysis, we found that: (i) The genes coding for the Pi transporter, acid phosphatase and RNase were up-regulated in rice roots; (ii) the genes involved in glycolysis were first up-regulated and then down-regulated; (iii) several genes involved in N metabolism and lipid metabolism changed their expression patterns; (iv) some genes involved in cell senescence and DNA or protein degradation were up-regulated; and (v) some transmembrane transporter genes were up-regulated. The results may provide useful information in the molecular process associated with Pi deficiency and thus facilitate research in improving Pi utilization in crop species.</p>","PeriodicalId":49127,"journal":{"name":"Science in China. Series C, Life Sciences / Chinese Academy of Sciences","volume":"52 11","pages":"1055-64"},"PeriodicalIF":0.0,"publicationDate":"2009-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s11427-009-0137-x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28527941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Origin and domestication history of Peking ducks deltermined through microsatellite and mitochondrial marker analysis.","authors":"LuJiang Qu,&nbsp;Wei Liu,&nbsp;FangXi Yang,&nbsp;ZhuoCheng Hou,&nbsp;JiangXia Zheng,&nbsp;GuiYun Xu,&nbsp;Ning Yang","doi":"10.1007/s11427-009-0145-x","DOIUrl":"https://doi.org/10.1007/s11427-009-0145-x","url":null,"abstract":"<p><p>In order to elucidate the domestication history of Peking ducks, 190 blood samples from six Chinese indigenous duck breeds were collected with 186 individuals genotyped by 15 microsatellite markers. Both the F(ST) and Nei's standard genetic distances (D(s)) from the microsatellite data indicated high genetic differentiation between Peking duck and other Chinese indigenous breeds. The haplotype network with mtDNA data showed that most of the Peking duck haplotypes were distinctly different from those of other domestic breeds. Although the H01 haplotype was shared by all domesticated duck breeds, Peking ducks displayed 12 specific domestic duck haplotypes, including four similar haplotypes H02, H04, H08 and H22, that formed a single haplogroup (A). Both H02 and H22 haplotypes were also shared by mallard and Peking ducks, indicating that Peking ducks originated from wild mallard ducks.</p>","PeriodicalId":49127,"journal":{"name":"Science in China. Series C, Life Sciences / Chinese Academy of Sciences","volume":"52 11","pages":"1030-5"},"PeriodicalIF":0.0,"publicationDate":"2009-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s11427-009-0145-x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28527493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}