The Indian Journal of Animal Reproduction最新文献

Impact of Cysteine as a Semen Additive on Mitigating the Decline in Sperm Quality due to Summer Stress in Riverine Buffaloes (Bubalus bubalis) 半胱氨酸作为精液添加剂对缓解水牛夏季应激导致的精子质量下降的影响

The Indian Journal of Animal Reproduction Pub Date : 2023-01-01 DOI: 10.48165/ijar.2023.44.01.7

J Arunpandian, Neeraj Srivastava, Gyanendra Singh, Brijesh Kumar, Amala Jackson, Pradeep Chandra, Subrato Kumar Ghosh, Sanjay Kumar Singh, Meiraj Haider Khan

{"title":"Impact of Cysteine as a Semen Additive on Mitigating the Decline in Sperm Quality due to Summer Stress in Riverine Buffaloes (Bubalus bubalis)","authors":"J Arunpandian, Neeraj Srivastava, Gyanendra Singh, Brijesh Kumar, Amala Jackson, Pradeep Chandra, Subrato Kumar Ghosh, Sanjay Kumar Singh, Meiraj Haider Khan","doi":"10.48165/ijar.2023.44.01.7","DOIUrl":"https://doi.org/10.48165/ijar.2023.44.01.7","url":null,"abstract":"The seasonal variations such as temperature, rain and humid environment greatly influences reproduction, and semen quality particularly in buffalo bulls, either positively or negatively. The heat stress coupled with freezing and thawing processes generates reactive oxygen species (ROS), which further reduce post-thaw spermatozoa quality. Application of cysteine as a semen additive have been reported to alleviate such damaging effect in canine, buck, bull, human, boar, and rooster. Therefore, objective of this study was to investigate ameliorating effect of cysteine on summer stress on buffalo spermatozoa. For the experiment, 24 ejaculates with individual progressive motility >70, volume >1mL and sperm concentration 1000 million/mL from four Murrah buffalo bulls were collected and divided into four groups as Control (Group-I), and Group-II, III, and IV (4, 8, and 12 mmol cysteine, respectively) containing 80 million spermatozoa per mL. Semen was filled (French mini straw), sealed and equilibrated at 40⸰C and frozen into LN2. The group containing L-cysteine at 4 mmol concentration had higher significant (p<0.05) difference in post thaw motility, viability, plasma membrane integrity and acrosome integrity, TAC, DNA integrity and lower significant (p<0.05) difference in spermatozoa abnormality, LPO and intracellular ROS. Moreover, cysteine concentration at 8 mmol has no beneficial effect compared to the Control, whereas Group-IV had greater adverse effect. This may be due to its toxicity at this concentration. It is evident from the study that the cysteine molecules at 4 mmol concentration produced more pronounced improvement in the freezability potential of buffalo bull spermatozoa.","PeriodicalId":488871,"journal":{"name":"The Indian Journal of Animal Reproduction","volume":"9 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135400197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Haemato-biochemical evaluation of chemotherapy in canine transmissible venereal tumour 犬传染性性病肿瘤化疗的血液生化评价

The Indian Journal of Animal Reproduction Pub Date : 2023-01-01 DOI: 10.48165/ijar.2023.44.01.5

Aja Taj Nazer, B Bibin Becha, C Jayakumar, M P Unnikrishnan, Suma-Thankachy Sreekantan-Thampi Devi

{"title":"Haemato-biochemical evaluation of chemotherapy in canine transmissible venereal tumour","authors":"Aja Taj Nazer, B Bibin Becha, C Jayakumar, M P Unnikrishnan, Suma-Thankachy Sreekantan-Thampi Devi","doi":"10.48165/ijar.2023.44.01.5","DOIUrl":"https://doi.org/10.48165/ijar.2023.44.01.5","url":null,"abstract":"The study was aimed at haematological and biochemical evaluation of chemotherapeutic response in canine transmissible venereal tumour (CTVT). Thirty-one CTVT confirmed dogs presented to University Veterinary Hospitals were categorised into three groups. The group I (n=13) dogs were treated with vincristine sulphate, while group II (n=9) and III (n=9) dogs were treated with vincristine along with ivermectin and cyclophosphamide respectively. Haemato-biochemical evaluations were performed on the day of presentation and at weekly intervals till complete tumour regression. The haemato-biochemical parameters like TEC, Hb, VPRC, TP, BUN, creatinine, albumin, globulin and ALT were within normal range. Significant differenceswere observed in DLC, TC, MCV, AST, ALP and bilirubin levels (p<0.05) during treatment. Various blood dyscrasias like leucocytosis was observed in group I and group II, and thrombocytopenia in group III. Highly elevated AST, ALP and bilirubin were observed in all three groups. But combination therapy with ivermectin was more efficient with fewer adverse effects and decreased treatment duration.","PeriodicalId":488871,"journal":{"name":"The Indian Journal of Animal Reproduction","volume":"156 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135400231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Blood Plasma Minerals and Biochemical Profile of Anestrus and Endometritic Cows Reared in Tribal Areas of Himachal Pradesh, India 印度喜马偕尔邦部落地区饲养的未发情和子宫内膜异位奶牛血浆矿物质和生化特征

The Indian Journal of Animal Reproduction Pub Date : 2023-01-01 DOI: 10.48165/ijar.2022.43.2.11

Ankita Sharma, Madhumeet Singh, Pravesh Kumar

{"title":"Blood Plasma Minerals and Biochemical Profile of Anestrus and Endometritic Cows Reared in Tribal Areas of Himachal Pradesh, India","authors":"Ankita Sharma, Madhumeet Singh, Pravesh Kumar","doi":"10.48165/ijar.2022.43.2.11","DOIUrl":"https://doi.org/10.48165/ijar.2022.43.2.11","url":null,"abstract":"Blood samples were collected from 227 infertile (Endometritis; n=123, Anoestrus; n=104) cows from five different tribal locations of Himachal Pradesh viz. Lahaul, Spiti, Kinnaur, Pangi and Bharmour. Significantly, lower levels of potassium were recorded in the Kinnaur district in both anestrus (3.33±0.09mmol/L) and endometritic (3.30±0.12mmol/L) cows as compared to other tribal areas. Significantly, higher levels of AST (p<0.01) were found in anestrus cows of Pangi (103.76±6.07 U/L) followed by Bharmour (93.34±14.43 U/L). The levels of cholesterol were significantly higher in anestrus cows of Lahaul (189.63±16.99 mg/dl) followed by Bharmour (184.54±19.57 mg/dl) and Pangi (162.03±13.83 mg/dl). The levels of creatinine were significantly higher (p<0.05) in anestrus cows of Spiti as compared to other tribal areas. The levels of ALT were significantly higher in anestrus cows of Spiti (37.22±2.01 U/L) followed by Pangi (34.44±3.19 U/L) and Lahaul (29.46±7.35 U/L). In the case of endometritic cows, values of ALT were significantly higher in Spiti (42.41±2.64 U/L) followed by Lahaul (23.37±4.9 U/L). The levels of total protein were found significantly higher in endometritic (8.34±0.31g/dl) cows of Lahaul as compared to other tribal areas.","PeriodicalId":488871,"journal":{"name":"The Indian Journal of Animal Reproduction","volume":"49 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135400913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ovarian Dynamics and Blood Flow Indices during Estrus in Purnathadi Buffaloes 普尔纳塔迪水牛发情期卵巢动力学和血流指数

The Indian Journal of Animal Reproduction Pub Date : 2023-01-01 DOI: 10.48165/ijar.2023.44.01.3

Aboli Kathe, Maheshkumar Vitthalarao Ingawale, Shyam Ganesh Deshmukh, Chaitanya Hemednrakumar Pawshe, Sunil Wasudeo Hajare, Ranjit Suresh Ingole

引用次数: 0

Management of Dystocia Due to Ventrotransverse Presentation of Fetus in a Mare 母马腹横位胎儿难产的处理

The Indian Journal of Animal Reproduction Pub Date : 2023-01-01 DOI: 10.48165/ijar.2023.44.01.16

Kalpesh Kumar Pargi, Dinesh Jhamb, Madhu Meena, Mitesh Gaur, Naveen Kumar

引用次数: 0

Effect of 2 Day vs. 3 Day Superstimulation Protocol on In Vivo Maturation of OPU derived oocytes in Buffaloes 2天和3天超刺激方案对水牛OPU衍生卵母细胞体内成熟的影响

The Indian Journal of Animal Reproduction Pub Date : 2023-01-01 DOI: 10.48165/ijar.2023.44.01.4

Narinder Singh, Gurjot Kaur Mavi, Ajeet Kumar, V S Malik

{"title":"Effect of 2 Day vs. 3 Day Superstimulation Protocol on In Vivo Maturation of OPU derived oocytes in Buffaloes","authors":"Narinder Singh, Gurjot Kaur Mavi, Ajeet Kumar, V S Malik","doi":"10.48165/ijar.2023.44.01.4","DOIUrl":"https://doi.org/10.48165/ijar.2023.44.01.4","url":null,"abstract":"The study was planned to evaluate effect of two different superstimulation protocols (2 Day vs. 3 Day) on in vivo maturation of oocytes in buffaloes. Buffaloes (n=8) were superstimulated twice using 2x2 design at an interval of 30-35 days using 200 mg and 300 mg FSH divided into four and six tapering doses (each dose of 50 mg) given over 2 and 3 days at 12 hourly intervals. Prostaglandin F2α analogue (500 μg Cloprostenol; i/m) at 72 and 84 hrs after start of superstimulatory treatment. Oocytes were retrieved from > 6 mm follicles at 48 h after the last FSH injection by ovum pick up. Oocyte maturation was assessed by the degree of cumulus cell expansion and by Orcein staining of denuded oocytes. OPU of 60 and 91 follicles resulted in recovery of 38 and 50 cumulus oocyte complexes (COCs) in 2-Day and 3-Day group, respectively. Significantly higher proportion of expanded oocytes was recovered in 3-Day protocol compared to 2-Day protocol (36.0% vs. 13.2%) and higher number of compact COCs were recovered in 2-Day protocol. In 3-Day protocol, significantly higher percentage of oocytes retrieved was at Metaphase-II stage compared to 2-Day protocol (40% vs.15.8%, respectively). The preliminary trial demonstrated that 3-day superstimulatory protocol yielded higher percentage of in vivo matured oocytes than 2-Day protocol in buffaloes.","PeriodicalId":488871,"journal":{"name":"The Indian Journal of Animal Reproduction","volume":"232 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135401826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

TWIN MUMMIFICATION IN NON-DESCRIPT DOE: A CASE REPORT 双胞胎木乃伊在非描述doe:一个案例报告

The Indian Journal of Animal Reproduction Pub Date : 2023-01-01 DOI: 10.48165/ijar.2023.44.01.17

Jisna Kuttiyeri Sasidharan, Shashikant Gupta, Pradeep K, Pradeep Chandra, Brijesh Kumar, Subrata Kumar Ghosh

引用次数: 0

Effect of percoll density gradient centrifugation on semen quality of X- sperm enriched crossbred bull semen percoll密度梯度离心对富含X精子的杂交公牛精液质量的影响

The Indian Journal of Animal Reproduction Pub Date : 2023-01-01 DOI: 10.48165/ijar.2023.44.01.11

Deeksha Chaudhary, Mridula Sharma, Kamal Devlal

{"title":"Effect of percoll density gradient centrifugation on semen quality of X- sperm enriched crossbred bull semen","authors":"Deeksha Chaudhary, Mridula Sharma, Kamal Devlal","doi":"10.48165/ijar.2023.44.01.11","DOIUrl":"https://doi.org/10.48165/ijar.2023.44.01.11","url":null,"abstract":"The present study was conducted to observe the effect of percoll density gradient centrifugation on quality of semen. Ejaculates were collected by AV method from Sahiwal bulls. X-sperm enrichment was done by percoll density gradient method i.e. 7 layers (70-10%). Centrifugation was done at 750 g (22-24°C) for 15 min. The pellets obtained were diluted in EYC medium. Semen quality was evaluated in fresh semen (Control), in pellet of normal centrifugation (Group I), supernatant of centrifugation in percoll density gradient (Group II) and pellet of centrifugation in percoll density gradient (Group III). To assess the quality of enriched semen pH, mass motility, progressive motility, live spermatozoa %, abnormal spermatozoa %, HOST % and intact acrosome % were evaluated. Number of progressively motile sperms in pellet of X- enriched semen were non-significantly increased and significantly (P<0.05) decreased in supernatant. The abnormal spermatozoa (%) were decreased in G III as compared to G II Live spermatozoa (%) were increased in enriched semen (pellet). Number of Intact sperms decreased significantly (P<0.05) in supernatant of percoll density gradient centrifuged Sahiwal semen. HOST responsive sperms number was not affected after percoll density gradient centrifugation. Thus, the semen quality of X sperm enriched semen by percoll density gradient method (7 layer 70%) was not affected hence it can be used to increase female calves’ birth after A.I.","PeriodicalId":488871,"journal":{"name":"The Indian Journal of Animal Reproduction","volume":"108 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135400238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Management of dystocia due to multiple mal-dispositions in a Marwari mare 马尔瓦里母马多重性征所致难产的处理

The Indian Journal of Animal Reproduction Pub Date : 2023-01-01 DOI: 10.48165/ijar.2022.43.2.19

Amit Kumar, Jugraj Singh Mahal, Amarjeet Bisla, Mrigank Honparkhe

引用次数: 0

Microbial Prevalence and Antimicrobial Sensitivity in Dairy Animals Suffering From Purulent Vaginal Discharge 患有脓性阴道分泌物的奶牛的微生物患病率和抗菌药物敏感性

The Indian Journal of Animal Reproduction Pub Date : 2023-01-01 DOI: 10.48165/ijar.2022.43.2.4

Gyan Singh, Ritu Yadav, Sandeep Kumar, Hitesh K, Anand Kumar Pandey

{"title":"Microbial Prevalence and Antimicrobial Sensitivity in Dairy Animals Suffering From Purulent Vaginal Discharge","authors":"Gyan Singh, Ritu Yadav, Sandeep Kumar, Hitesh K, Anand Kumar Pandey","doi":"10.48165/ijar.2022.43.2.4","DOIUrl":"https://doi.org/10.48165/ijar.2022.43.2.4","url":null,"abstract":"Uterine infection can be diagnosed by taking a swab from fornix vagina or directly from cervix and can be used for cytology and microbiological evaluation. In this study, vaginal swabs for culture and sensitivity test were obtained from 20 buffaloes and two cows which were shedding purulent pus discharge from their reproductive tract. The most isolated microorganism was Escherichia coli which were detected in 17 animals, followed by Staphylococcus spp. and Streptococcus spp. which was detected in 15 and 14 animals, respectively. Klebsiella spp and Baccilus spp. were diagnosed in two animals whereas; Psuedomonas spp. and Candida spp. were also diagnosed in one animal. Regarding sensitivity tests, the most efficient antibiotics were Gentamicin 54.5% (12/22) followed by Ciprofloxacin 50% (11/22), Enrofloxacin 45% (10/22), Neomycin 45% (10/22), Amikacin 45% (10/22), Cefoperazone 36% (8/22), Levofloxacin 31% (7/22), Norfloxacin 27% (6/22), Chloramphenicol 27% (6/22) and Cephalexin 22.7% (5/22). Likewise, the most resistant antibiotics in descending order were Streptomycin 95.4% (21/22), Penicillin and Cloxacillin 90.9% (20/22), Amoxicilin, Moxifloxacin and Ampicillin 86% (19/22), Oxytetracycline and Ceftriaxone 81.8% (18/22), Cephalexin 72.7% (16/22), Levofloxacillin 68% (15/22), Carbenicillin, Chloramphenicol, Kanamycin and Cefoperazone 63.6% (14/22), Tobramycin, Neomycin and Enrofloxacin 54.5% (12/22), Amikacin and Ciprofloxacin 50% (11/22), Cephotaxime 45.5 (10/22), Norfloxacin and Gentamycin 40.9% (9/22), Clindamycin and Septran 22.7% (5/22), Amoxiclav and Erythromycin 18 % (4/22). The present study concluded that the most prevalent microorganisms isolated from uterine discharge, were found to be resistant to beta-lactam antimicrobials as well, the study highlighted the significance of performing microbiological analyses as well as sensitivity tests prior to applying an antimicrobial therapy.","PeriodicalId":488871,"journal":{"name":"The Indian Journal of Animal Reproduction","volume":"156 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135400236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0