Rossijskij bioterapevtičeskij žurnal最新文献

{"title":"Study of biocompatibility and antitumor cytotoxic activity <i>in vitro</i> of Zn – 1 %Mg and Zn – 1 %Mg – 0.1 %Ca alloys strengthened by equal angular pressing","authors":"N. S. Martynenko, N. Yu. Anisimova, O. V. Rybalchenko, M. V. Shinkareva, D. R. Temralieva, K. M. Novruzov, A. G. Raab, E. A. Lukyanova, E. A. Kornyushenkov, D. V. Filonenko, D. E. Mitrushkin, S. V. Dobatkin","doi":"10.17650/1726-9784-2023-22-3-64-74","DOIUrl":"https://doi.org/10.17650/1726-9784-2023-22-3-64-74","url":null,"abstract":"Introduction. The biological activity of potential biodegradable zinc-based alloys that are promising for oncoorthopedics was studied in this work. The alloys were processed by equal-channel angular pressing, which made it possible to increase their strength due to microstructure refinement and the ability to provide the functionality of osteosynthesis, fixed due to the metal structure developed on their basis. Aim . Investigation of effect of equal-channel angular pressing (ECAP) treatment on strength, ductility, degradation rate, biocompatibility in vitro and cytotoxicity against SKOV-3 tumor cells of the Zn – 1 %Mg and Zn – 1 %Mg – 0.1 %Ca alloys. Materials and methods. The Zn – 1 %Mg and Zn – 1 %Mg – 0.1 %Ca alloys in the initial state and after ECAP were used as objects of study, and blood cells of CBA mice were used as model systems. To assess the hemolytic activity, the samples were incubated with red blood cells for 4 and 24 hours at 37 °C, assessing the relative increase in the level of extracellular hemoglobin compared to the intact control. The cytotoxicity of the alloys was assessed by the change in the level of extracellular lactate dehydrogenase (LDH) activity after 24 hours of incubation with mononuclear white blood cells. The study of antitumor cytotoxic activity was carried out on human ovarian cancer cells of the SKOV-3 line in vitro, assessing their survival after 48 hours of incubation with alloy samples using the LDH test. Results . As a result of the studies, it was concluded that the studied alloys after ECAP treatment retained their biocompatibility, since there were no signs of hemolysis and cytotoxicity with respect to blood cells. However, contact with samples of all studied alloys in vitro induced a significant inhibition of the metabolic activity of the ovarian cancer cell culture in comparison with the control. Incubation with alloys samples leads to a decrease in cellular activity by an average of 49 % and 59 % for Zn – 1 %Mg and Zn – 1 %Mg – 0.1 %Ca alloys, respectively. The addition of calcium to the composition of the alloy Zn – 1 %Mg contributed to the growth of antitumor cytotoxic activity. Conclusion. Thus, based on the results of assessing the hemolytic activity and cytotoxicity of the samples, we can conclude that the studied alloys are biocompatible. It was also found that Zn – 1 %Mg and Zn – 1 %Mg – 0.1 %Ca alloys had a pronounced cytotoxic effect on SKOV-3 tumor cells. The obtained data indicate the prospects for the development of a new type of medical devices based on the studied alloys, promising, in particular, for oncoorthopedics: a metal structure developed on their basis can ensure the strength of osteosynthesis, reduce the risk of local recurrence of oncological disease and does not require a second operation to remove the device.","PeriodicalId":488723,"journal":{"name":"Rossijskij bioterapevtičeskij žurnal","volume":"22 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135943191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Changes in urine metabolite concentration as a minimally invasive marker of ovarian serous adenocarcinoma","authors":"O. N. Guskova, I. A. Alliluev, E. V. Verenikina, V. V. Polovodova, M. A. Rogozin, T. Yu. Myagkova, M. L. Adamyan, O. E. Zhenilo, N. M. Abdullaeva, M. R. Tsandekova, N. D. Ushakova, D. S. Kutilin","doi":"10.17650/1726-9784-2023-22-3-43-50","DOIUrl":"https://doi.org/10.17650/1726-9784-2023-22-3-43-50","url":null,"abstract":"Introduction. Detection of ovarian cancer (OC) at the earliest possible stages is a priority for gynecological oncology, since 5-year survival rates decrease significantly with the progression of the disease. Currently, there is a huge need for more effective diagnostic methods and approaches. In recent years, fluid biopsy has received increasing attention in precision medicine because it is minimally invasive and can be repeated many times, allowing for realtime disease monitoring. Aim. Study of the urine metabolomic profile of patients with ovarian carcinoma. Materials and methods. To perform metabolomic analysis, 50 urine samples from patients with a diagnosis of serous ovarian carcinoma and 20 samples from apparently healthy individuals were selected. For protein precipitation, 300 mkl of urine was mixed with 600 mkl of a solution of acetonitrile LC-MS (Merck, Germany) and methanol LC-MS (Merck, Germany) (3:1 ratio). Chromatographic separation of metabolites was performed on a Vanquish Flex UHPLC System chromatograph (Thermo Scientific, Germany). The chromatograph was coupled to an Orbitrap Exploris 480 mass spectrometer (Thermo Scientific, Germany) equipped with an electrospray ionization source. Chromatographic separation was carried out on a Hypersil GOLD™ C18 column (1.9mkm, 10 x 2.1 mm) using the following eluents: A, 0.1 % formic acid; B, acetonitrile containing 0.1 % formic acid. Results. A total of 417 metabolites of various classes were identified by HPLC-MS. It was shown that in the urine of patients with OC 14 metabolites (kynurenine, phenylalanyl-valine, lysophosphatidylcholine (18:3), lysophosphatidylcholine (18:2), alanyl-leucine, lysophosphatidylcholine (20:4), L-phenylalanine, phosphatidylinositol (34:1), 5-methoxytryptophan, 2-hydroxymyristic acid, 3-oxocholic acid, lysophosphatidylcholine (14:0), indoleacrylic acid, lysophosphatidylserine (20:4)) had a significantly higher concentration compared to apparently healthy individuals. The content of 12 compounds, on the contrary, was reduced (L-beta-aspartyl-L-phenylalanine, myristic acid, decanoylcarnitine, aspartyl-glycine, malonylcarnitine, 3-hydroxybutyrylcarnitine, 3-methylxanthine, 2,6-dimethylheptanoylcarnitine, 3-oxododecanoic acid, N-acetylproline, L-octanoylcarnitine, capryloylglycine). This indicates a significant metabolomic imbalance in patients with OC. Conclusion . The metabolomic profile study of urine by UHPLC-MS showed that in patients with serous ovarian carcinoma there is an imbalance in the content of certain fatty acids and their derivatives, acylcarnitines, phospholipids, amino acids and their derivatives, as well as some derivatives of nitrogenous bases. At the same time, 26 metabolites with abnormal concentrations in urine may have some potential as non-invasive biomarkers of OC in women belonging to high-risk groups.","PeriodicalId":488723,"journal":{"name":"Rossijskij bioterapevtičeskij žurnal","volume":"56 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135943193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Schisandrin and Schisantherin A in Multiphytoadaptogen for Preventive Oncology","authors":"O. A. Bocharova, I. V. Kazeev, V. E. Shevchenko, N. S. Ionov, O. P. Sheichenko, E. V. Bocharov, R. V. Karpova, V. G. Kucheryanu, A. A. Lagunin, D. A. Filimonov, V. S. Kosorukov, V. V. Poroikov, V. A. Tutelyan, N. V. Pyatigorskaya","doi":"10.17650/1726-9784-2023-22-3-51-63","DOIUrl":"https://doi.org/10.17650/1726-9784-2023-22-3-51-63","url":null,"abstract":"Background . The original herbal formula of Multiphytoadaptogen (MPhA) for preventive oncology developed by the N. N. Blokhin Center of Oncology containing phytocomponents from Schisandra chinensis (Turcz.) Baill (Schisandraceae), has been investigated in vitro, in vivo. Preliminary efficiency in clinical trials has also been obtained. This was allowed because MPhA in Russia is registered as a parapharmaceutical agent and therefore standardized according to established requirements. However, due to the high efficiency of MPhA, a detailed study of the chemical composition and standardization of it is required, including the Schisandra chinensis active components, which turned out to be translocated into MPhA as a result of the extraction technology developed. The aim of the study was to identify the Schisandra chinensis biologically active substances in MPhA and to evaluate the biological activity profiles of the identified phytocomponents using in silico analysis. Materials and methods. we used high performance liquid chromatography in combination with mass spectrometry (HPLC–MS / MS). Chromatography was performed on an ACQUITY UPLC BEH C18 column in a gradient mode. A TSQ Vantage triple quadrupole mass spectrometer with electrospray ionization was used. we performed in silico analyzes of Schisandrin and Schisantherin A biological activity spectra using computer programs PASS and PharmaExpert. Result . The secondary metabolites lignans Schisandrin and Schisantherin A were identified in the herbal formula MPhA. Schisandrin and Schisantherin A activities, according the scientific literature and in silico analysis, correspond to the properties studied for MPhA which therefore fits into the concept of medication for preventive oncology. Conclusion. The determined secondary metabolites can be used for identification, standardization and quality testing of the herbal formula MPhA.","PeriodicalId":488723,"journal":{"name":"Rossijskij bioterapevtičeskij žurnal","volume":"18 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135943194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combined photodynamic and radiodynamic therapy with a chlorine photosensitizer under fractionated radiation exposure to transplanted tumors in an &lt;i&gt;in vivo&lt;/i&gt; experiment","authors":"D. A. Tzerkovsky, A. N. Mazurenko, D. I. Kozlovsky, N. D. Adamenko, F. F. Borichevsky","doi":"10.17650/1726-9784-2023-22-3-75-86","DOIUrl":"https://doi.org/10.17650/1726-9784-2023-22-3-75-86","url":null,"abstract":"Introduction. The current direction of scientific research in experimental and clinical oncology is radiodynamic therapy (RDT), a treatment method based on the activation of photosensitizers (PS) by ionizing radiation. As the results of in vitro / in vivo studies show, a number of photosensitizing agents have radiomodifying properties, and the method itself is characterized by high antitumor efficacy. Aim. To study the possibility of increasing the effectiveness of photodynamic therapy (PDT) through its combined use with RDT in an in vivo experiment. Materials and methods. The work was performed on 26 white non-linear rats weighing 180 ± 50 g. Pliss lymphosarcoma was used as a tumor model, which was transplanted subcutaneously. The chlorine-based PS (Belmedpreparaty, Republic of Belarus) was administered intravenously at a dose of 2.5 mg / kg of body weight. The RDT session was performed by the contact method (CRT) after 2.5–3, 24 and 48 hours after the end of PS administration on the microSelectron-HDR V3 Digital device (Elekta, Sweden) using γ-radiation (192Ir) in a single focal dose (SFD) of 2 Gy until the total focal dose of 6 Gy is reached. A PDT session was performed once immediately after the first exposure to ionizing radiation using a PDT diode laser (Imaf Axicon, Republic of Belarus, λ = 660 ± 5 nm) at an exposure dose of 100 J / cm² with a power density of 0.2 w / cm² and a power of 0.353 w. All rats were divided into 4 groups of 6–7 animals each: intact control, PS + PDT, PS + CRT, PS + CRT + PDT. The criteria for evaluating antitumor efficacy were: the average volume of tumors (Vav), the coefficient of absolute growth of tumors (K), the coefficient of tumor growth inhibition (TGI), the frequency of complete tumor regressions (CR), the proportion of cured rats, an increase in the average duration of dead rats. Differences were considered statistically significant at p <0.05. Results. On the 18th day of the experiment, Vav in groups was 65.98 ± 0.81 cm³; 24.02 ± 4.01 cm³ (р <0.00001); 17.11 ± 4.13 cm³ (р <0.00001); 4.42 ± 1.65 cm³ (р <0.00001), respectively. Coefficients K – 5497.33 OE; 1714.71 OE; 1424.83 OE; 367.33 OE. TGI coefficients – 63.60 % (PS + PDT); 74.07 % (PS + CRT); 93.30 % (PS + CRT + PDT). An in crease in the average duration of dead rats indicator – 25.31 % (PS + PDT); 55.06 % (PS + CRT); 158.22 % (PS + CRT + PDT). On the 60th and 90th days of the experiment, the frequency of CR and the proportion of cured rats were the same and amounted to 0; 16.7; 14.3 and 42.9 %, respectively. Conclusion. The results obtained indicate that the chlorine PS has, in addition to photosensitizing properties, also radiomodifying properties, and the developed method of combined therapy is characterized by high antitumor efficacy.","PeriodicalId":488723,"journal":{"name":"Rossijskij bioterapevtičeskij žurnal","volume":"5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135943192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Features of regulation of hepcidin and ferroportin in cancer patients (literary review)","authors":"V. N. Blindar, G. N. Zubrikhina, T. V. Davydova, M. M. Dobrovolskaya","doi":"10.17650/1726-9784-2023-22-3-10-18","DOIUrl":"https://doi.org/10.17650/1726-9784-2023-22-3-10-18","url":null,"abstract":"Background. The pathways of iron acquisition, outflow, storage and regulation are disrupted in cancer, which suggests that the reprogramming of iron metabolism is one of the central aspects of the survival of tumor cells. Aim. Is to review and generalize modern literature data on the regulation of hepcidin, ferroportin and prospects for the correction of iron metabolism in cancer patients. Materials and Methods. The paper presents the results of international and domestic studies of the peculiarities of iron metabolism and the prospects for its correction in cancer patients. The search for relevant sources was carried out in the web of Science, PubMed, Medline, eLibrary.ru systems for 1988–2023. Of the analyzed studies 61, the most relevant, were used to write a systematic review. Results . Over the past decade, a new understanding has emerged of the role of proteins, in particular hepcidin and ferroportin, which regulate cellular iron in cancer growth, angiogenesis and metastasis. New treatment methods with hepcidin-modifying strategies and stabilizers of hypoxia-induced factors are emerging, but their therapeutic efficacy for correcting iron metabolism in cancer patients needs to be evaluated and clinical trials. Conclusion. Analysis of the literature data has shown the high relevance of studies of the regulation of hepcidin and ferroportin in cancer patients and the need for further study of this problem.","PeriodicalId":488723,"journal":{"name":"Rossijskij bioterapevtičeskij žurnal","volume":"16 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136038506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"mRNA of the &lt;i&gt;OY-TES-1&lt;/i&gt; gene in peripheral blood and tumors in colorectal cancer","authors":"N. R. Hilal, D. V. Novikov, V. N. Yakimov, R. G. Pegov, V. V. Novikov","doi":"10.17650/1726-9784-2023-22-3-28-35","DOIUrl":"https://doi.org/10.17650/1726-9784-2023-22-3-28-35","url":null,"abstract":"Background. One of the testicular cancer genes is the OY-TES-1 gene, which encodes a proacrosin-binding protein. This gene is known to be expressed in tumor cells. Aim. To determinate the detection frequency of OY-TES- 1 matrix ribonucleic acid (mRNA) in the peripheral blood and tumor of patients with colorectal cancer (CRC) in comparison with the clinical features of the course of a disease in order to assess the potential monitoring significance of this indicator. Materials and methods. A method for determining the level of OY-TES-1 mRNA based on reverse transcription polymerase chain reaction (RT-PCR). The detection frequency of mRNA OY-TES-1 gene in blood and tumor samples of 66 patients with CRC, as well as the blood of healthy volunteers, was evaluated. Results. In the peripheral blood of healthy volunteers, no OY-TES-1 mRNA was detected. In the blood of CRC patients, OY-TES-1 mRNA was detected in 13.6 % of cases. In tumors, it was found in 60,6 % of cases. OY-TES -1 mRNA in the blood and in tumor of patients with CRC was detected statistically significantly more often at stages III and IV than at stages I and II (11,0 and 4,5 times, respectively), and also many times more often in tumors with a low degree of differentiation than with highly differentiated tumors. In patients with CRC, the OY-TES-1 mRNA in tumors with metastases in organs and lymph nodes was detected many times more often than in tumors without any metastases (by 16,4 and 17,4 times respectively). Conclusion. The data obtained indicate the absence of mRNA OY-TES-1gene in the blood of healthy individuals, the appearance of mRNA OY-TES-1 gene in the blood of CRC patients, and the possibility of using the detection of mRNA OY-TES-1 gene in the blood as an additional informative monitoring marker for colorectal cancer.","PeriodicalId":488723,"journal":{"name":"Rossijskij bioterapevtičeskij žurnal","volume":"19 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136038503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of a status of microsatellite instability in tumors of patients with endometrioid adenocarcinoma of the ovaries and/or of uterine corpus","authors":"A. S. Aniskina, J. G. Payanidi, A. M. Stroganova, I. V. Manina, K. I. Zhordania","doi":"10.17650/1726-9784-2023-22-3-36-42","DOIUrl":"https://doi.org/10.17650/1726-9784-2023-22-3-36-42","url":null,"abstract":"Introduction. Multiple primary malignant neoplasms of female reproductive organs are a rare pathology. However, over the past decades, there has been an upsurge of interest in the study of this phenomenon in oncology. This is particularly the case for the diagnosis of synchronous endometrioid adenocarcinoma of the ovaries and uterine corpus, which histogenetically belong to the same germ layer and have similar histological structure. Until recently, clinicians relied only on morphological examination in these cases, but with the development of molecular genetic technologies, new diagnostic possibilities have emerged. Aim. Is the detection of the status of microsatellite instability in tumors of patients with endometrioid adenocarcinoma of the ovaries and/or uterine corpus. Materials and Methods. A pilot retrospective molecular genetic study ( n = 48) was conducted to determine the status of microsatellite instability (MSI) in the tumors of the ovaries and/or uterine corpus: it involved 33 patients with solitary endometrioid ovarian cancer and 15 patients with synchronous endometrioid adenocarcinoma of the ovaries and uterine corpus. Microsatellite instability status was detected using PCR method with subsequent fragment analysis performed on ABI PRISM 3500 genetic analyzer (8 capillaries, Applied Biosystems). DNA was isolated from paraffin blocks of surgical specimens using DNAsorb B extraction kit (AmpliSens, Russia), according to the manufacturer’s manual. DNA concentration was estimated fluorometrically using Qubit 2.0 (Life Technologies, USA). The obtained data were analyzed using GeneMapper program (Thermo Fisher, USA). In case of polymorphism of two and more markers high-level microsatellite instability (MSI-H) was observed. Results. The incidence of MSI-H in solitary endometrioid ovarian cancer ( n = 33) was 12,1 % (4 cases), while in synchronous ovarian and uterine corpus tumors ( n = 15) MSI-H incidence made up 20 % ( n = 3). Herewith, there have been only cases of a combination of endometrioid histotypes of ovarian and endometrial cancer with identical status of microsatellite instability. Thus, the incidence of MSI-H in synchronous endometrioid adenocarcinoma of the ovaries and uterine corpus (20 %) is comparable to that in solitary endometrial cancer. Conclusion. Our pilot study became a significant complement to the previously published materials, as it allowed to confirm the clonal origin of tumors in patients with endometrioid adenocarcinoma of the ovaries and uterine corpus, that can affect the stratification of treatment strategy for this category of patients.","PeriodicalId":488723,"journal":{"name":"Rossijskij bioterapevtičeskij žurnal","volume":"221 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136038505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mannose antibody levels in gastric cancer patients (literature review and clinical and experimental study)","authors":"M. P. Nikulin, N. V. Shilova, A. D. Lipatnikov, A. V. Semyanikhina, I. S. Stilidi, N. V. Bovin, N. N. Tupitsyn","doi":"10.17650/1726-9784-2023-22-3-19-27","DOIUrl":"https://doi.org/10.17650/1726-9784-2023-22-3-19-27","url":null,"abstract":"Introduction. Glycans play an important role in the pathogenesis of malignant neoplasms, including stomach cancer. In recent years, the attention of many researchers has been drawn to mannose (Man) – hexose, which is an indispensable component of all N-chains of glycoproteins involved in both normal physiological and pathological processes. Aim. To investigate the role of innate immunity factors and ways to influence them through mannose and mannose-containing glycans in gastric cancer patients. Materials and methods. Data on the role of mannose – one of the key monosaccharides in the formation of glycoprotein N-chains – and its binding receptors (mannose receptor, mannose-binding lectin, antibodies) in gastric cancer since 2006 are presented. Levels of anti-glycan antibodies in blood serum samples of 235 gastric cancer patients and 76 healthy donors were evaluated using a glycochip. Results. It has been shown that the level of IgM-class antibodies to Manβ – the core part of N-glycans – in gastric cancer patients is significantly lower compared to the donor group, regardless of age (p = 0.0001). To assess the effect of age on the levels of antiglycan antibodies, patients were divided into two subgroups – before and after 45 years. In the group under 45 years of age, significant differences in the levels of antiglycan antibodies to Manβ persisted, while significant differences in the levels of antiglycan antibodies to Manβ1-4GlcNAcβ were not observed. when comparing groups of patients and donors older than 45 years, the levels of antibodies to Manβ and Manβ1-4GlcNAcβ were significantly higher only in donors. Conclusion. Deficiency of humoral immunity may be one of the key factors in the initiation and progression of carcinogenesis in humans. In our work, in patients with stomach cancer, we revealed a deficiency of antiglycan antibodies to Manβ and to Manβ1-4GlcNAcβ – core fragments of N-chains of glycoproteins, and the deficiency increased with age. The results of the study are a promising platform for further research aimed not only at studying the role of anti-mannose antibodies, but also at developing approaches to adoptive immunoprophylaxis.","PeriodicalId":488723,"journal":{"name":"Rossijskij bioterapevtičeskij žurnal","volume":"220 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136038504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}