mRNA of the <i>OY-TES-1</i> gene in peripheral blood and tumors in colorectal cancer

Abstract

Background. One of the testicular cancer genes is the OY-TES-1 gene, which encodes a proacrosin-binding protein. This gene is known to be expressed in tumor cells. Aim. To determinate the detection frequency of OY-TES- 1 matrix ribonucleic acid (mRNA) in the peripheral blood and tumor of patients with colorectal cancer (CRC) in comparison with the clinical features of the course of a disease in order to assess the potential monitoring significance of this indicator. Materials and methods. A method for determining the level of OY-TES-1 mRNA based on reverse transcription polymerase chain reaction (RT-PCR). The detection frequency of mRNA OY-TES-1 gene in blood and tumor samples of 66 patients with CRC, as well as the blood of healthy volunteers, was evaluated. Results. In the peripheral blood of healthy volunteers, no OY-TES-1 mRNA was detected. In the blood of CRC patients, OY-TES-1 mRNA was detected in 13.6 % of cases. In tumors, it was found in 60,6 % of cases. OY-TES -1 mRNA in the blood and in tumor of patients with CRC was detected statistically significantly more often at stages III and IV than at stages I and II (11,0 and 4,5 times, respectively), and also many times more often in tumors with a low degree of differentiation than with highly differentiated tumors. In patients with CRC, the OY-TES-1 mRNA in tumors with metastases in organs and lymph nodes was detected many times more often than in tumors without any metastases (by 16,4 and 17,4 times respectively). Conclusion. The data obtained indicate the absence of mRNA OY-TES-1gene in the blood of healthy individuals, the appearance of mRNA OY-TES-1 gene in the blood of CRC patients, and the possibility of using the detection of mRNA OY-TES-1 gene in the blood as an additional informative monitoring marker for colorectal cancer.