Research Journal of Biotechnology最新文献_第2页

Emergence of Highly Mercury Tolerant Plant Growth promoting Bacteria in Tea Plantation Soil of Darjeeling Hills 大吉岭山区茶园土壤中出现的高耐汞植物生长促进细菌

IF 0.2

Research Journal of Biotechnology Pub Date : 2024-02-29 DOI: 10.25303/1904rjbt014023

S. Acharyya, S. Saha, M. Bhattacharya

引用次数: 0

Toxicity evaluation of a strain isolated from pollack digestive tract using Paramecium caudatum 用尾柱虫评估从鳕鱼消化道分离的菌株的毒性

IF 0.2

Research Journal of Biotechnology Pub Date : 2024-02-29 DOI: 10.25303/1904rjbt096099

Rim Song-guk, Hong Chol, Ri Yong-jin, Nam Kyong-ryong, Kim Hui-won

{"title":"Toxicity evaluation of a strain isolated from pollack digestive tract using Paramecium caudatum","authors":"Rim Song-guk, Hong Chol, Ri Yong-jin, Nam Kyong-ryong, Kim Hui-won","doi":"10.25303/1904rjbt096099","DOIUrl":"https://doi.org/10.25303/1904rjbt096099","url":null,"abstract":"A strain was isolated from the digestive organs of Walleye pollack residing in the East Sea of the DPR Korea, which not only produces cryophilic protease, organic acid but also has intestinal adhesion property and bacteriostatic activity against pathogens. The strain was classified and identified as Pseudomonas fragi by genetic system classification methods and registered as KCCC 10110 at the Korean Centre for Culture Collection (KCCC) of the State Academy of Sciences, DPR KOREA. In vivo and in vitro toxicity of Pseudomonas fragi KCCC 10110 was evaluated using Paramecium caudatum. In the treatments with P. fragi KCCC 10110, Bacillus subtilis KCCC 11214 and Lactobacillus lactis KCCC 13208, all of these being well known as non-toxic and probiotic for animals and fishes, higher microbial counts and normal movement of P.caudatum were observed while significant lower cell numbers and abnormal movement of P.caudatum were shown in the treatments with pathogenic strains Clostridium tetani 473, Salmonella typhosa 901 and Aeromonas punctata 823. These results suggest that probiotic and pathogenic bacteria have different influence on Paramecium caudatum and that the strain isolated from Walleye pollack digestive tract is non-toxic.","PeriodicalId":48695,"journal":{"name":"Research Journal of Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2024-02-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140415804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A comprehensive review of the ecological significance, pharmacological potential and economic importance of Samanea saman 关于萨马尼亚槐的生态意义、药理潜力和经济重要性的全面综述

IF 0.2

Research Journal of Biotechnology Pub Date : 2024-02-29 DOI: 10.25303/1904rjbt1420145

S. Krupa, Gnanesh Rao, N. Laksmikanth, N. Mohan

引用次数: 0

Indole acetic acid (IAA) production by endophytic bacterial isolate Agrobacterium tumefaciens BE-1 from roots of Musa acuminata 从尖叶麝香根中分离出的内生细菌农杆菌 BE-1 产生的吲哚乙酸（IAA）

IF 0.2

Research Journal of Biotechnology Pub Date : 2024-02-29 DOI: 10.25303/1904rjbt032039

M. Ambawade, D. D. Patil, G. R. Pathade, Gajanan V. Mali

引用次数: 0

Bioinoculants for growth enhancement of aromatic plants: Artemisia pallens and Origanum majorana 促进芳香植物生长的生物接种剂：苍术和牛至

IF 0.2

Research Journal of Biotechnology Pub Date : 2024-02-29 DOI: 10.25303/1904rjbt1000106

Veda Sai Kalavalapalli, Meghana Kolati, Aishwarya Bandaru, P. R. Gyana

{"title":"Bioinoculants for growth enhancement of aromatic plants: Artemisia pallens and Origanum majorana","authors":"Veda Sai Kalavalapalli, Meghana Kolati, Aishwarya Bandaru, P. R. Gyana","doi":"10.25303/1904rjbt1000106","DOIUrl":"https://doi.org/10.25303/1904rjbt1000106","url":null,"abstract":"The use of fertilizers is a common practice to improve soil fertility and increase crop yields. Chemical fertilizers have played a significant role in increasing crop yields, but their widespread use has led to environmental degradation and soil depletion. The continuing challenges in agro-ecosystem and environment require more sustainable solutions than chemical fertilizers. In recent years, there has been a growing interest in the use of biofertilizers as a sustainable alternative to chemical fertilizers. Biofertilizers are natural substances that improve soil fertility, modify soil microbiota and enhance plant growth. Cyanobacteria are one of the well-known bio inoculants for paddy crops. However, the use of cyanobacteria for other plants has not been very well documented. We screened certain cyanobacteria isolated from different natural environments as biofertilizers for certain aromatic plants. Artemisia pallens and Origanum majorana are two plants with aromatic compound productions. The aim of this study was to screen for enhancement in the growth and propagation of the plants for their yield using bio inoculants. Further air-dried, powdered plant materials of the aerial parts of A pallens and O. majorana were extracted using solvents ranging in polarity from non-polar (n-hexane), semi-polar (chloroform) and polar (acetone, methanol). These extracts were tested for antioxidant activity, several plant extracts showed antioxidant activity, with water exhibiting the greatest levels. The purpose of the experiment was to investigate the effects of bioinoculants on the growth of the test plant. Our observation showed that all the tested cyanobacterial strains i.e. KM1, KM2 and VM1, VM2, the bioinoculants, enhanced plant growth to various levels. KM1 and KM2 exhibited a greater ability to increase plant growth. This demonstrates that Artemisia's and Origanum’s growth and herbage output may be successfully enhanced by the use of cyanobacterial bioinoculants.","PeriodicalId":48695,"journal":{"name":"Research Journal of Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2024-02-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140413906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quantitative Phytochemicals and Antioxidant Potential of Adansonia digitata leaves and stem extract Adansonia digitata叶和茎提取物的定量植物化学物质和抗氧化潜力

IF 0.2

Research Journal of Biotechnology Pub Date : 2024-02-29 DOI: 10.25303/1904rjbt05013

Chhavi Goel, R. Dutta

{"title":"Quantitative Phytochemicals and Antioxidant Potential of Adansonia digitata leaves and stem extract","authors":"Chhavi Goel, R. Dutta","doi":"10.25303/1904rjbt05013","DOIUrl":"https://doi.org/10.25303/1904rjbt05013","url":null,"abstract":"The objective of this study was to examine the phytochemical properties of the baobab tree (Adansonia digitata) stems and leaves. The dried powder of leaves and stem extract were analyzed for the presence or absence of different phytochemicals, flavonoids, terpenoids, saponins, tannins, alkaloids etc. The quantitative analysis revealed that the leaves and stem of A. digitata are rich in total phenols, alkaloids, terpenoids, flavonoids and saponins. The presence of these compounds was known to show medicinal potential as well as antioxidant activity and this justifies the use of baobab leaves as one of the major sources of soup in Africa. Chlorophyll (chl.) estimation was also studied by two different methods in fresh leaves and dried leaves. Fresh leaves have high chlorophyll content of 2.0 mg/g while shade and sun-dried leaves have content 1.664 mg/g and 1.047 mg/g respectively. With DMSO, high chlorophyll content of fresh leaves is 2.240 mg/g and for shade and sun-dried leaves, it is 1.695 mg/g and 1.526 mg/g respectively. This represents that high chlorophyll and phytochemicals present in plants show high antioxidant properties. Subsequently, antioxidant activity at different concentrations 62.5, 125, 250, 500, 750 μg/ml of the extract was established using FRAP assay and DPPH action radical scavenging activity methods respectively. Ethanolic extract shows higher antioxidant activity than hydroethanolic extract.","PeriodicalId":48695,"journal":{"name":"Research Journal of Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2024-02-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140414229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Botulinum toxin as a therapy for cerebral palsy 肉毒杆菌毒素治疗脑瘫

IF 0.2

Research Journal of Biotechnology Pub Date : 2024-02-29 DOI: 10.25303/1904rjbt1350141

S.A. Raj, S. Adithya, A. Shanker, F. Ahmad

引用次数: 0

Identification of novel PPAR-γ agonist ameliorating triple negative breast cancer by in silico methods 通过硅学方法鉴定可改善三阴性乳腺癌的新型 PPAR-γ 激动剂

IF 0.2

Research Journal of Biotechnology Pub Date : 2024-01-31 DOI: 10.25303/1903rjbt058069

A. Laxmi, Jeena Gupta, Pawan Gupta

{"title":"Identification of novel PPAR-γ agonist ameliorating triple negative breast cancer by in silico methods","authors":"A. Laxmi, Jeena Gupta, Pawan Gupta","doi":"10.25303/1903rjbt058069","DOIUrl":"https://doi.org/10.25303/1903rjbt058069","url":null,"abstract":"Triple negative breast cancer (TNBC) is still considered a medical challenge as it is an aggressive cancer characterized by poor prognosis, frequent metastasis, distinct clinical and pathological features and lack of proper treatment options. In TNBC, estrogen, progesterone and Her2 neu receptors are absent, which are usually present in otherwise breast cancers. So, hormonal therapy cannot be given in case of TNBC, leaving chemotherapy as the mainstay for treatment. However, chemotherapy is an infamous therapeutic option due to several aweful side effects like loss of appetite, extreme weight loss, hair loss, vomiting, fatigue etc. Several reports have highlighted role of Peroxisome Proliferated Activated Receptor gamma (PPAR-γ) in ameliorating breast cancer, but it is still less explored receptor for TNBC. Moreover, synthetic PPAR gamma agonists like thiazolidinediones, have good capability of activating PPAR-γ receptor, but these are also gloomy therapeutic agent due to their dreadful side effects like hepatotoxicity, bladder cancer, congestive heart failure etc. So, there is serious urge among researchers to find safer therapeutic option for TNBC. Phytochemicals are nowadays grabbing attention worldwide to treat several diseases due to their fewer or no side effects. This in silico study was performed after thorough review to select a natural, potent PPAR-γ agonist Gallotanin, as a query compound for ligand based similarity searching in PubChem database with 80% filter. Top 10 obtained compounds having highest similarity index were chosen for molecular docking in AutoDock Vina, with PPAR-γ receptor (3V9T) to know their binding patterns. Further, top 5 compounds having highest dock scores underwent ADME studies in SwissADME to assess pharmacokinetic profile of these compounds. Finally, this in silico study resulted in discovery of compound called Chamuvaritin which is still not known as a PPAR-γ agonist, efficient enough to act as potent therapeutic agent against TNBC.","PeriodicalId":48695,"journal":{"name":"Research Journal of Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2024-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140476936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

De novo transcriptome analysis of Argemone mexicana plant (SRR287649) and annotation for secondary metabolite producing genes Argemone mexicana 植物（SRR287649）的全新转录组分析和次生代谢物产生基因的注释

IF 0.2

Research Journal of Biotechnology Pub Date : 2024-01-31 DOI: 10.25303/1903rjbt086092

Raghunath Satpathy

引用次数: 0

Simple and Rapid PCR-RFLP based species identification in Drosophila suzukii and Drosophila immigrans larvae 基于 PCR-RFLP 的简单快速的铃木果蝇和移栖果蝇幼虫物种鉴定

IF 0.2

Research Journal of Biotechnology Pub Date : 2024-01-31 DOI: 10.25303/1903rjbt048050

Shwetha Karaningannavar, Rajat Hegde, Ramesh Babu Yarajarla

{"title":"Simple and Rapid PCR-RFLP based species identification in Drosophila suzukii and Drosophila immigrans larvae","authors":"Shwetha Karaningannavar, Rajat Hegde, Ramesh Babu Yarajarla","doi":"10.25303/1903rjbt048050","DOIUrl":"https://doi.org/10.25303/1903rjbt048050","url":null,"abstract":"Wild fruit fly larvae can be distinguished at the family level such as Drosophilidae and Tephritidae larvae are based on size or spiracles arrangement, but such visual distinction is more intricate between Drosophilid species. In the present study, to identify the Drosophila immigrans and Drosophila suzukii species at the larval stage, we employed a simple, rapid PCR-RFLP experiment. Wild suspected larvae from Drosophila immigrans and Drosophila suzukii species were collected from 3 different geographical regions. DNA was isolated and a PCR-RFLP profile was developed using the XmnI restriction enzyme. Sequencing of the mitochondrial COI gene from larvae was performed to evaluate the species. PCR-RFLP analysis from Drosophila suzukii wild larvae generated three distinct bands with 120bp, 210bp and 250bp. Similarly, Drosophila immigrans generated 2 distinct bands with 270bp and 310bp. Restriction digestion products from both the larvae and adult flies were the same, hence the accuracy of the PCR-RFLP profile developed from both suspected wild larvae and adult flies was 100%. Sequencing analysis confirms that wild field collected larvae belong to Drosophila suzukii and Drosophila immigrans. Present study clearly shows that PCR‐RFLP is a fast, simple, inexpensive and accurate molecular method and it can be successfully used for species identification at the early stages of the life cycle of Drosophila species.","PeriodicalId":48695,"journal":{"name":"Research Journal of Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2024-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140474830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0