{"title":"Evaluation of Combinational Therapy of L-Arginine with Some Phenolic Acids in Paclitaxel-induced Neuropathic Pain","authors":"Shubhangi Pawar, Amrapali Pagare, Suvarna Katti, Rupali Patil, Manisha Tayde","doi":"10.5530/ajbls.2023.12.39","DOIUrl":"https://doi.org/10.5530/ajbls.2023.12.39","url":null,"abstract":"Background: Paclitaxel is an anticancer drug that can induce neuropathy and is used in animal models to evaluate new therapeutic approaches in neuropathy. Paclitaxel increases oxidative stress, which is a key contributor to the pathogenesis of neuropathy. Many phenolic acids as well as amino acids with antioxidant potential are proven as neuroprotective. Objectives: To evaluate combinational therapy of L-Arginine with Syringic acid and Sinapic acid, separately in paclitaxel-induced neuropathy by assessing behavioral and biochemical parameters. Materials and Methods: Wistar rats were divided into seven groups (n=6). Neuropathic pain was developed with Paclitaxel (2mg/kg, i.p), repetitively administered on the 1st, 3rd, 5th, and 7th days in rats. Treatment with phenolic acids, L-Arginine and Gabapentin was started along with paclitaxel from day first for 5 weeks. Behavioural study (mechanical and thermal hyperalgesia, allodynia and motor co-ordination) was carried out weekly by using Von Frey filaments, Rota rod apparatus, hot plate and cold plate. Biochemical study (MDA, GSH, SOD) was performed after sacrification at the end of the study. Results: A combination of L-Arginine with syringic acid and sinapic acid has shown a significant reversal of behavioural changes and oxidative stress induced by paclitaxel. L-Arginine has potentiated individual effects of syringic acid and sinapic acid. Conclusion: The results suggested antioxidant, analgesic and neuroprotective effects in combinational therapy of L-Arginine. This study is indicating that combination of L-Arginine could be beneficial to reduce dose requirement of syringic acid and sinapic acid in treatment of neuropathic pain.","PeriodicalId":483927,"journal":{"name":"Asian journal of biological and life sciences","volume":"41 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136142801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Bahnishikha Singh, Debasmita Chatterjee, Sanket Bandyopadhyay, Satadal Das
{"title":"Ultra-diluted Arsenic Trioxide Induced Cytokine Changes in HepG2 Cell Line","authors":"Bahnishikha Singh, Debasmita Chatterjee, Sanket Bandyopadhyay, Satadal Das","doi":"10.5530/ajbls.2023.12.43","DOIUrl":"https://doi.org/10.5530/ajbls.2023.12.43","url":null,"abstract":"As an alternative medicine ultra-diluted arsenic is used for problems in the digestive tract, upset stomach, sleep disorders, allergies, psoriasis, syphilis, asthma, disorders in the muscles, joints and bones, haemorrhoids, cough, pruritus, cancer, and pain. In this study, we are interested in observing cytokine expression changes which may help some understanding of the proper use of such medicine. Due to a similarity in the expression of alterations in the chemical activity of drug or integral transmembrane proteins in the cells on various metabolic pathways, the use of HepG2 cells as an experimental model cell line for such study of hepatocytes is well known. Cytopathic Effects (CPE), MTT assay, DNA fragmentation, apoptotic gene expressions, and cytokine gene expressions caused by ultra-diluted arsenic on HepG2 cells were studied. The cytokine environment of the challenged HepG2 cells was delineated by a quantitative Real-Time Polymerase Chain Reaction (qRT-PCR) study to observe gene expression changes compared to control gene β-actin. All findings indicated a strong apoptotic gene expression change caused by this medicine on HepG2 cells. There were rounding of the cells in CPE, non-viable findings in methylene blue staining, cytotoxic nature in MTT assay, and DNA-fragmentations indicated gross cellular damage. There was an up-regulation of pro-inflammatory cytokines and a down-regulation of anti-inflammatory cytokines with increased gene expression of interferon-gamma. In conclusion, ultra-diluted arsenic can potentially alter the expression of apoptotic genes and different cytokine genes and also induce an apoptotic pathway in the HepG2 cells","PeriodicalId":483927,"journal":{"name":"Asian journal of biological and life sciences","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136142280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Biosynthesis of Zinc Oxide Nanoparticles from Soil Fungi and their Characterization by Using Analytical Methods","authors":"Amitha Ganapathy Bottangada, Akarsh Subhakar, Sowmya Hirakannavar Veeranna, Thippeswamy Basaiah","doi":"10.5530/ajbls.2023.12.36","DOIUrl":"https://doi.org/10.5530/ajbls.2023.12.36","url":null,"abstract":"Aims: The main aim of the study is to synthesize the zinc oxide nanoparticles from fungal isolates of soil and characterize the physicochemical properties of synthesized ZnO NPs for agricultural purposes. Materials and Methods: The soil samples have been collected, isolated, and examined for the solubilization of zinc. The promising isolate was molecular characterized and deposited in NCBI GenBank. This isolate was then utilized to synthesize and characterize ZnO nanoparticles. The synthesized ZnO NPs were characterized using UV-vis DRS, UV-vis spec, DLS, XRD, FT-IR, and SEM-EDAX for confirmation and quantification. Results: In the 14 fungal isolates, Aspergillus fumigatus (Db5) has shown the highest zinc solubilization index of 2.75mm. The fungal culture filtrate displayed an absorption peak at 315 nm in UV-visible spectrum analysis whereas, in UV-vis DRS, it showed 330nm with a band gap found to be ~3.1 eV, which indicated the presence of zinc oxide. The synthesized ZnO-NPs measure 27.35 nm in particle size and have a zeta potential of -2.35 mV. The average size of the ZnO NPs was found to be 23nm through XRD. The functional hydroxyl (O-H) group of the ZnO NP showed an intense band near 3447.16 cm-1 through FT-IR analysis. In SEM studies, the morphology of ZnO NPs was found to be irregular, spherical, and granule-like structures. Conclusion: All the studies indicate that the fungal isolate Aspergillus fumigatus (Db5) can synthesize ZnO NPs which can be further tested for crop protection and production as an eco-friendly approach.","PeriodicalId":483927,"journal":{"name":"Asian journal of biological and life sciences","volume":"28 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136143007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Millano Millano M, Jan Gabrielle, Moril Moril M, Katherine Katherine M, Muro Muro M, Jose Ricardo, Narvaez Narvaez N, Jenelle Audrey, Nieves Nieves N, Ma Lindsay Joy, Nino Nino N, Eloisa Jane, Palo Palo P, Ronuel Kenn, Prias Prias P, Ma Catherine Anne Jhennifer, Bremner Bremner B, Pamela Rose
{"title":"A Systematic Review of the Anti-proliferative Activity of Algae-synthesized Silver Nanoparticles against Cancer Cell Lines","authors":"Millano Millano M, Jan Gabrielle, Moril Moril M, Katherine Katherine M, Muro Muro M, Jose Ricardo, Narvaez Narvaez N, Jenelle Audrey, Nieves Nieves N, Ma Lindsay Joy, Nino Nino N, Eloisa Jane, Palo Palo P, Ronuel Kenn, Prias Prias P, Ma Catherine Anne Jhennifer, Bremner Bremner B, Pamela Rose","doi":"10.5530/ajbls.2023.12.31","DOIUrl":"https://doi.org/10.5530/ajbls.2023.12.31","url":null,"abstract":"Context: Chemotherapeutic agents and radiation therapy have been acknowledged as the sole cancer treatment, even though they cause significant adverse reactions. Recent research has demonstrated that treatment-based nanoparticles can specifically target cancer cells, thereby overcoming these detrimental effects. Nanotechnology is a promising new anti-cancer treatment since it focuses primarily on the cytotoxic processes that induce apoptosis in cancerous cells. Algae, which can be subdivided into microalgae and macroalgae, appear to be the most suitable substrate for nanoparticle synthesis among the various plant divisions utilized thus, for the bioreduction of metal nanoparticles. Objectives: This review focuses on the essential elements and techniques involved in the algal-mediated synthesis of AgNPs, as well as the cytotoxicity of these biosynthesized silver nanoparticles generated by microalgae and macroalgae against various forms of human cancer. Data Sources: Data were acquired utilizing a variety of online databases, including ScienceDirect, PubMed Central, Springer Link, National Center for Biotechnology Information, and ResearchGate. The accumulating literature highlights the unique properties of AgNPs produced by various algae, their method of anti-proliferative action, and their cytotoxic evaluation of several cancer cell lines. Results: This review found that the antiproliferative effect of macroalga and microalga biosynthesized silver nanoparticles is dependent on particle size, concentration, and cancer cell lines tested. Since their high growth rate and short harvesting technique without causing damage to normal cells, macroalgae are more typically used to synthesize AgNPs than microalgae in this research.","PeriodicalId":483927,"journal":{"name":"Asian journal of biological and life sciences","volume":"14 7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136142417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The Effect of High Fructose Corn Syrup on SCC-9 Cells Regulated by G0/G1 Cell Cycle Arrest via Apoptosis Induction","authors":"Kalimuthu Kavitha, Ganapathy Sindhu, Marimuthu Porkodi, Kumarasamypillai Radha Thayammal Asha, Veeran Veeravarmal, Annamalai Vijayalakshmi","doi":"10.5530/ajbls.2023.12.51","DOIUrl":"https://doi.org/10.5530/ajbls.2023.12.51","url":null,"abstract":"","PeriodicalId":483927,"journal":{"name":"Asian journal of biological and life sciences","volume":"22 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136142456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Comparison of Tree Species Diversity and Usage by Ratufa indica indica in Umblebyle Range Forest, Bhadravathi Division, Shimoga, Karnataka, India","authors":"Hamsa Rekha Venkatesh, Shwetha Alavandi","doi":"10.5530/ajbls.2023.12.33","DOIUrl":"https://doi.org/10.5530/ajbls.2023.12.33","url":null,"abstract":"Ratufa indica is an arboreal top canopy-dwelling species endemic to India. Tree species diversity of a forest plays an important role in the survival of arboreal species including Ratufa indica, hence the study was designed to analyze the tree species diversity of dry deciduous forests of the Umblebyle Range, Shimoga, Karnataka. The study was carried out using line transect methodology surveying 20 transects walking a total distance of 47.7 km of trees covering an area of 8350.89 ha. A total of 77 tree species belonging to 31 families were identified. Nest tree preferences were assessed through the observation of 406 and 455 dreys (nests) on 385 and 415 nesting trees belonging to 20 and 22 families in the year 2021 and 2022 respectively. The animal was found to use the most abundant families and tree species 47 among 77 tree species were used for nesting, Fabaceae and Terminalia paniculata were the most preferred family and tree species, further deciduous trees were most preferred over others. The tree species diversity influences the nesting tree species diversity of the animal maintaining tree species diversity not only aids the conservation of animals in their habitat but also aids in the conservation of the forests","PeriodicalId":483927,"journal":{"name":"Asian journal of biological and life sciences","volume":"13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136142105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Patricia Andrea I Valenciano, Amante C Soriano III, Hazel Mae L Sisican, Eloisa Fami I Paragas, Kayla T Tabarina, Kaycelyn B Ramos, Charlene Princess S Tolenada
{"title":"The Prevalence of Soil-Transmitted Helminths (STH) and Entamoeba spp. Infections in Southeast Asia: A Systematic Review","authors":"Patricia Andrea I Valenciano, Amante C Soriano III, Hazel Mae L Sisican, Eloisa Fami I Paragas, Kayla T Tabarina, Kaycelyn B Ramos, Charlene Princess S Tolenada","doi":"10.5530/ajbls.2023.12.30","DOIUrl":"https://doi.org/10.5530/ajbls.2023.12.30","url":null,"abstract":"Background: Soil-Transmitted Helminths (STH) infection and Entamoeba species, specifically Entamoeba histolytica are known to infect billions worldwide – creating a global health problem; continues to be an international concern due to poor sanitation and unhygienic practices. The primary objective of this review is to evaluate the presence, prevalence, and trend of STH and Entamoeba spp., specifically, A. lumbricoides, T. trichiura, N. americanus, A. duodenale, E. histolytica, and E. dispar in Southeast Asia. Materials and Methods: Materials and methods include gathering and evaluating peer-reviewed and credible journal articles that have met the set standards for this review through PRISMA. Results: Among the fourteen journals reviewed, the most prevalent parasite in Southeast Asia is A. lumbricoides, with 36%, followed by T. trichiura garnered 35%, Hookworms, specifically, N. americanus and A. duodenale come third with 26%, and lastly, Entamoeba spp., specifically, E. histolytica and E. dispar come last with 3% rating. Along with this, the review also found that the Philippines garnered the highest-tallied intestinal parasitic infection, while Cambodia has the lowest number of recorded infections in the reviewed journals. Furthermore, the trend of parasitic infection in Southeast Asia was found to be highest in 2017. Conclusion: This review found that common factors such as poor sanitary conditions, and poor hygiene practices, along with tropical to warm and humid weather affect the longevity of the STH and Entamoeba species life cycle in Southeast Asia.","PeriodicalId":483927,"journal":{"name":"Asian journal of biological and life sciences","volume":"224 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136142241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"In vitro Study of Ixora coccinea Fruit Extracts for their Antioxidant and Tyrosinase Inhibitory Activities","authors":"Sindhu Rani J A","doi":"10.5530/ajbls.2023.12.57","DOIUrl":"https://doi.org/10.5530/ajbls.2023.12.57","url":null,"abstract":"Aim: The aim of the study is to investigate the antioxidant and antityrosinase properties of Ixora coccinea Linn. fruit extracts from various solvents. Materials and Methods: The antioxidant property of the extract was tested by using 2,2 Diphenyl-1-picryl hydrazyl (DPPH) radical scavenging assay, antityrosinase activity by mushroom tyrosinase inhibitory method, and phenol estimation by Folin- Ciocalteu method. Results: The qualitative phytochemical screening confirmed the presence of alkaloids, flavonoids, tannins, phenols, and glycosides, in the fruit extracts. In vitro, antioxidant analysis showed that the methanolic fruit extract has good free radical scavenging activity when compared with standard Ascorbic acid and also exhibited potent tyrosinase inhibition using Kojic acid as a standard tyrosinase inhibitor. Conclusion: The present study proved that the methanolic fruit extract of Ixora coccinea has significant antioxidant and skin-whitening properties, and can be used for making skin care formulations that are safe and cost effective.","PeriodicalId":483927,"journal":{"name":"Asian journal of biological and life sciences","volume":"12 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136142457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Anne Jolina Eustaquio Doria, Crislyn Joy Saballa Dalusong, Roma Alheia Mendoza De Castro, Tricia Nicole Malabanan Dioneda, Kyzha Zen Buencuchillo Estefanio, Liv Andrea Perez Galvez, Maria Fe Bago Gregorio, Pamela Rose Flores Bremner
{"title":"Comparative Evaluation on the Staining Potential of Garcinia mangostana (Mangosteen) and Selenicereus guatemalensis (Dragon Fruit) for Ova of Trichuris suis and Strongyloides ransomi","authors":"Anne Jolina Eustaquio Doria, Crislyn Joy Saballa Dalusong, Roma Alheia Mendoza De Castro, Tricia Nicole Malabanan Dioneda, Kyzha Zen Buencuchillo Estefanio, Liv Andrea Perez Galvez, Maria Fe Bago Gregorio, Pamela Rose Flores Bremner","doi":"10.5530/ajbls.2023.12.56","DOIUrl":"https://doi.org/10.5530/ajbls.2023.12.56","url":null,"abstract":"The conventional stains used for the detection of parasitic helminths are hazardous to laboratory scientists, who are exposed to iodine. A potential alternative stain to detect helminth ova is found in this study. This investigates the possibility of a natural dye between Garcinia mangostana (Mangosteen) and Selenicereus guatamalensis (Dragon Fruit) extracts. These fruits contain anthocyanin and betacyanin pigments that stain from purplish to brownish colour. The experiment utilizes distilled water and ethanol with two different concentrations to assess the effectivity of the colour of the pigments in helminth detection. The concentrations tested included 50% ethanol extract of mangosteen, 50% ethanol extract of dragon fruit, 50% distilled water extract of mangosteen, and 50% distilled water extract of dragon fruit. Statistical analysis revealed no significant difference in staining potential between the stain extracts and Lugol’s Iodine. However, further analysis indicated that 50% ethanol extract of mangosteen and 50% ethanol extract of dragon fruit exhibited better staining compared to their distilled water counterparts. The pH values of the stains were also measured, with Lugol’s Iodine having a pH value of 6.0, and the ethanol extracts having a pH value of 5.0, while the distilled water extracts had a pH value of 4.0. Based on the results, it can be concluded that ethanol extracts possess the best staining capacity against distilled water extracts.","PeriodicalId":483927,"journal":{"name":"Asian journal of biological and life sciences","volume":"13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136142459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Enhancement of Production of Exopolysaccharides from Bacillus Species","authors":"Rajendrabhai Daulatbhai Vasait, Ahire Vanita Laxman, Sonawane Pooja Hemant, Deoghare Neha Subhash, Borse Archana Ashok","doi":"10.5530/ajbls.2023.12.48","DOIUrl":"https://doi.org/10.5530/ajbls.2023.12.48","url":null,"abstract":"It has been shown that spontaneously synthesized Exopolysaccharides (EPS) are employed for a variety of commercial uses. The kind of EPS generated by bacteria to defend themselves from unsafe circumstances. Food additives as a natural supply of carbohydrates and proteins, bio-emulsifiers, stabilizers, biosorbents, cosmetic preparations, anticancer agents, antioxidants, and biologically active antimicrobials are all examples of how EPS is employed. Because EPS generated by bacteria is at a significant commercial level, the current study focused on screening and isolating EPS-producing organisms from the rhizosphere soil of various plants. Four EPS-producing bacteria were isolated and investigated in this study. A medium to enhance EPS production from a Bacillus species, soil isolate, H, was optimized. In order to identify functional groups, FTIR spectrophotometry was employed. Furthermore, the presence of carbohydrates and proteins in EPS was detected subjectively as well as quantitatively. Higher carbohydrate and protein concentrations, such as 23.66 and 15.19 mg/dL, were found in the EPS produced from isolates F and O, respectively. The total protein content of isolate H, EPS was found to be 13.05 g/L. Finally, in presence of optimum conditions, isolate H produced 13.11 g/","PeriodicalId":483927,"journal":{"name":"Asian journal of biological and life sciences","volume":"246 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136142593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}