Menara Perkebunan: Jurnal Penelitian Bioteknologi Perkebunan最新文献

Viabilitas mikroba selulolitik pada media pod dan pulp kakao (Theobroma cacao L.) 舱底介质和可可纸浆中的纤维化微生物的活性。

Menara Perkebunan: Jurnal Penelitian Bioteknologi Perkebunan Pub Date : 2023-10-31 DOI: 10.22302/e-journalmenaraperkebunan.v91i2.552

Nisfatin Shofiana, Titi Candra Sunarti, Anja Meryandini

{"title":"Viabilitas mikroba selulolitik pada media pod dan pulp kakao (Theobroma cacao L.)","authors":"Nisfatin Shofiana, Titi Candra Sunarti, Anja Meryandini","doi":"10.22302/e-journalmenaraperkebunan.v91i2.552","DOIUrl":"https://doi.org/10.22302/e-journalmenaraperkebunan.v91i2.552","url":null,"abstract":"Cocoa production in Indonesia is increasing from year to year. Cocoa production increased has an impact on increasing waste cocoa pod and pulp. Cocoa pod contains lignocellulose, while cocoa pulp contains many sugars. The composition of cocoa pod and pulp allows the cellulolytic microbe to grow. This study aims to perform microbial selection from fermented cocoa and sugar cane can grow in cocoa pod and pulp (Theobroma cacao). The selection method using the CMC 1%, pod 1%, pulp 1%, and pod 0,5%+pulp 0,5% media. Then, the analysis of reducing sugar using DNS method, while the total sugar analysis using phenol-H2SO4 method. Sugars of hydrolisis result were analyzed quantitatively using TLC. The results of microbial selection on CMC 1%, pod %, pulp 1%, and pod 0,5%+pulp 0,5% media obtained TBT 3.2 have the largest cellulolytic index on the pod 1% media. Isolates TBT 3.2 grown on pod 1% medium produce reducing sugar is 4,965 mg/mL at 30 hours sampling with highest total sugar at 6 hours sampling is 9,789 mg/mL. The types of sugars identified using the TLC are mannose, galactose, glucose, and selobiose.","PeriodicalId":476735,"journal":{"name":"Menara Perkebunan: Jurnal Penelitian Bioteknologi Perkebunan","volume":" 37","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135863127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Propagation and shelf-life of weed pathogenic fungi in alternative media and their effectiveness in billygoat (Ageratum conyzoides L) 杂草病原菌在代用培养基上的繁殖、保存期及其对山山羊的影响

Menara Perkebunan: Jurnal Penelitian Bioteknologi Perkebunan Pub Date : 2023-10-31 DOI: 10.22302/e-journalmenaraperkebunan.v91i2.536

Loekas Soesanto, Murti Ragil Wisnu Sastyawan, Abdul Manan, Endang Mugiastuti

{"title":"Propagation and shelf-life of weed pathogenic fungi in alternative media and their effectiveness in billygoat (Ageratum conyzoides L)","authors":"Loekas Soesanto, Murti Ragil Wisnu Sastyawan, Abdul Manan, Endang Mugiastuti","doi":"10.22302/e-journalmenaraperkebunan.v91i2.536","DOIUrl":"https://doi.org/10.22302/e-journalmenaraperkebunan.v91i2.536","url":null,"abstract":"Control of billygoat (Ageratum conyzoides L) currently uses herbicides, on the other hand, control using pathogenic fungi is environmentally friendly, but for mass propagation and storage, alternative media are needed. The aim of the study was to determine the best type of alternative media for fungal growth and shelf life, as well as its effectiveness against billygoat. The treatment tested involves a combination of two factors: the type of pathogenic fungi (Curvularia lunata or Fusarium oxysporum) and the type of medium (rice washing water or tofu liquid waste). A completely randomized design was used for the in vitro test, while in planta test used a randomized block design with each experimental unit repeated five times. The observed variables were conidia density, number of colonies, incubation period, disease symptoms, disease intensity, and area under the disease progress curve (AUDPC), as well as weed height, number of leaves, fresh and dry shoot, and root weights. The results showed that the conidia density of F. oxysporum was 57% better in rice washing water than in tofu wastewater. The best shelf life for the fungus was four weeks. The use of rice washing water for F. oxysporum and C. lunata effectively delayed the incubation period by 77 and 71% respectively, suppressed disease intensity by 90 and 88%, and AUDPC by 94 and 93% compared to the control. The F. oxysporum grown on rice washing water media was able to reduce the number of leaves, fresh and dry weight of billygoat by 25, 30, and 20% compared to the control, respectively.","PeriodicalId":476735,"journal":{"name":"Menara Perkebunan: Jurnal Penelitian Bioteknologi Perkebunan","volume":"3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135872434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mitigation of 3-MCPDE and GE in palm oil in Indonesia 印度尼西亚棕榈油中3-MCPDE和GE的缓解

Menara Perkebunan: Jurnal Penelitian Bioteknologi Perkebunan Pub Date : 2023-10-31 DOI: 10.22302/mp.v91i2.549

Jenny Elisabeth

{"title":"Mitigation of 3-MCPDE and GE in palm oil in Indonesia","authors":"Jenny Elisabeth","doi":"10.22302/mp.v91i2.549","DOIUrl":"https://doi.org/10.22302/mp.v91i2.549","url":null,"abstract":"Among consumed-vegetable oils, RBDPO (refined, bleached and deodorized palm oil) is indicated containing higher level of 3-MCPDE and GE, which have been classified as potentially carcinogenic and/or genotoxic to human. Those contaminants in RBDPO are formed during deodorization process with high temperature and also correlate with chloride content and quality of CPO as raw material. The maximum limit for 3-MCPDE and GE content in vegetable oil has been set at 2500 and 1000 µg kg-1, and as low as 750 and 500 µg kg-1 when used for production of baby food and cereal-based foods for infants and young children. The mitigation of 3-MCPDE and GE in RBDPO is quite challenging for palm oil refineries in producing a good quality of palm oil in term of sensory, stability, safety, and nutritional value, especially in Indonesia. It has been proven that modification of conventional refining process can reduce the 3-MCPDE and GE content. They are including pre-treatment of CPO by water washing to reduce the chloride content, alkali neutralization to remove any acids present in the oil prior to deodorization, using neutral bleaching earth, adding antioxidants after bleaching to reduce free radical-mediated formation of 3-MCPDE and GE, lowering the deodorization temperature with a longer time, and even double bleaching and/or double deodorization of the palm oil. Post-refining treatment using specific adsorbents is also applied to remove the 3-MCPDE and GE. However, palm oil refineries have to apply combination of several mitigation strategies to adequately reduce the levels of 3-MCPDE and GE, including using good quality of CPO with low FFA, chloride, and oxidized components content.","PeriodicalId":476735,"journal":{"name":"Menara Perkebunan: Jurnal Penelitian Bioteknologi Perkebunan","volume":"435 ","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135863525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sterilization method of contaminated oil palm plantlets affects the survival rate during acclimatization 污染油棕幼苗的灭菌方法影响其驯化成活率

Menara Perkebunan: Jurnal Penelitian Bioteknologi Perkebunan Pub Date : 2023-10-30 DOI: 10.22302/e-journalmenaraperkebunan.v91i2.551

Masna Maya Sinta, Lailia Zubaidah, Rizka Tamania Saptari, Imron Riyadi, Galuh Wening Permatasari, Riza Arief Putranto, Annisa A Aksa, Larasati D Mahardhika, Yuli Setiawati, Hayati Minarsih, Ernayunita Ernayunita

{"title":"Sterilization method of contaminated oil palm plantlets affects the survival rate during acclimatization","authors":"Masna Maya Sinta, Lailia Zubaidah, Rizka Tamania Saptari, Imron Riyadi, Galuh Wening Permatasari, Riza Arief Putranto, Annisa A Aksa, Larasati D Mahardhika, Yuli Setiawati, Hayati Minarsih, Ernayunita Ernayunita","doi":"10.22302/e-journalmenaraperkebunan.v91i2.551","DOIUrl":"https://doi.org/10.22302/e-journalmenaraperkebunan.v91i2.551","url":null,"abstract":"Contamination in the in vitro culture is a critical problem causing the failure of seed production. Contamination in the oil palm plantlet is detrimental, considering that oil palm propagation is difficult and takes a long time. This research aimed to study the effect of sterilization during acclimatization of the contaminated oil palm plantlets by fungi on viability and to determine the optimum viability achieved from the contaminated materials. The materials used were contaminated plantlets of oil palm with roots, four leaves, and a height of about 17 cm. The plantlets were removed from the tube and cleaned with running tap water, then were sterilized, with treatments P1: soaking in benomyl-mancozeb-sodium hypochlorite and mannitol and rinsing with aquadest, P2: soaking in benomyl-mancozeb, P3: soaking in mancozeb. Cleaning plantlets under running tap water was carried out as a control treatment. The results showed that at 10 weeks after acclimatization, the survival rate of plantlets in each treatment (P1, P2, and P3) was significantly higher than that of the control. Sterilization methods affect the time new leaves emerge, leaf condition after sterilization treatment, and shoot height. The lowest fungal contamination after treatments was found in P2, followed by P3. After 3 months, plantlet survival rate decreased, with the highest survival rate in treatment P3 (32.3%) followed by treatment P2 (22.5%). In conclusion, acclimatization of contaminated oil palm plantlets can be carried out using a suitable sterilization treatment. Sterilization affects the survival rate and growth of in vitro-contaminated oil palm plantlets during acclimatization.","PeriodicalId":476735,"journal":{"name":"Menara Perkebunan: Jurnal Penelitian Bioteknologi Perkebunan","volume":"109 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136068973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pengaruh tiga jenis formula teknologi Sucrosin terhadap pertumbuhan dan produktivitas tebu (Saccharum officinarum L.) Sucrosin技术公式对甘蔗生长和生产力的三种影响(Saccharum officinarum L)。

Menara Perkebunan: Jurnal Penelitian Bioteknologi Perkebunan Pub Date : 2023-10-26 DOI: 10.22302/e-journalmenaraperkebunan.v91i2.545

Muhammad Abdul Aziz, Ciptadi Ahmad Yusup, Siswanto Siswanto, Djoko Santoso, Priyono Priyono, Happy Widiastuti

{"title":"Pengaruh tiga jenis formula teknologi Sucrosin terhadap pertumbuhan dan produktivitas tebu (Saccharum officinarum L.)","authors":"Muhammad Abdul Aziz, Ciptadi Ahmad Yusup, Siswanto Siswanto, Djoko Santoso, Priyono Priyono, Happy Widiastuti","doi":"10.22302/e-journalmenaraperkebunan.v91i2.545","DOIUrl":"https://doi.org/10.22302/e-journalmenaraperkebunan.v91i2.545","url":null,"abstract":"Sucrosin technology, have been tested in more than 10,000 ha of sugarcane planting area, resulted in highly variable increases in productivity. However, fulfilling standard operational procedures when implemented on a wide scale is complicated, so it needs to be simplified regarding product formulation and application techniques. This study aimed to evaluate the three different formulas of Sucrosin technology in increasing sugarcane growth and productivity. The study was conducted on PC sugarcane at RNI Plantation, Majalengka, West Java, using the Bululawang 1303 variety. The research design was a completely randomized design with four treatments consisting of Sucrosin technology existing (Se), formula 1 (S1), and formula 2 (S2) and control. The application of formula 2 Sucrosin technology (S2) could significantly increase the height and diameter of sugarcane stalks at 6 and 9 MAP. Sugarcane production in Se, S1, and S2 treatments increased by 22.49% (112.71 ton ha-1), 20.47% (110.85 ton ha-1), and 16.45% (107.15 ton ha-1) compared to controls (92.02 ton ha-1), respectively. These results show that Sucrosin Se technology produces the highest production. Nevertheless, from a technical perspective for implementation in the field, the most straightforward Sucrosin technology is S2. However, further research needs to be conducted in the broader area across different agro-ecologies to confirm the current findings.","PeriodicalId":476735,"journal":{"name":"Menara Perkebunan: Jurnal Penelitian Bioteknologi Perkebunan","volume":"114 4","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134907916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gibberellic acid (GA) role in acetyl-coA carboxylase enzyme regulation and in improving oil palm yield 赤霉素(GA)在调节乙酰辅酶a羧化酶和提高油棕产量中的作用

Menara Perkebunan: Jurnal Penelitian Bioteknologi Perkebunan Pub Date : 2023-10-25 DOI: 10.22302/e-journalmenaraperkebunan.v91i2.533

Irma Kresnawaty, Djoko Santoso, Galuh Wening Permatasari, Sumi Hudiyono

{"title":"Gibberellic acid (GA) role in acetyl-coA carboxylase enzyme regulation and in improving oil palm yield","authors":"Irma Kresnawaty, Djoko Santoso, Galuh Wening Permatasari, Sumi Hudiyono","doi":"10.22302/e-journalmenaraperkebunan.v91i2.533","DOIUrl":"https://doi.org/10.22302/e-journalmenaraperkebunan.v91i2.533","url":null,"abstract":"Seaweed specifically, Sargassum sp. is known to contains a boosting hormone growth that has been promoted plant growth and yield due to the containing of auxin, gibberelic acid (GA) and cytokinine, and also some amino acids especially glutamic acid. Those composition could be used as an booster of palm oil production which related to acetyl co-A carboxylase activity (ACC). ACC is the rate determination step in fatty acid accumulation, and becomes active through dephosphorylation of some serine residues that induced by magnesium and glutamate. Moreover, ACC was regulated by AtWRI1 and AtWRI1-TCP4 interaction, a mechanism that allow fine-tuning of the oil biosynthetic pathway. In this research we conducted gene expression experiments, and molecular docking analyses to study the possible mechanism of seaweed composition stimulating oil accumulation in the oil palm. Further analysis was conducted to ensure whether the interaction between TCP4 and candidate inhibitors were able to phosphorylate TCP4 and decrease its activity. GA application resulted in the increase of oil accumulation in 1 month after application, although in the second month the oil accumulation showed decreasing. Increase of oil accumulation in the first month in line with the increase of the expression of ACC in 3rd and 5th weeks. Meanwhile, TCP4 showed decrease expression that resulted in the increase of the WRI1 in 5th week. From this result, it was indicated that GA application could block the TCP4, so it could not interact with WRI1, resulted in the expression of WRI1 and ACC. This interaction stimulates the oil accumulation in oil palm.","PeriodicalId":476735,"journal":{"name":"Menara Perkebunan: Jurnal Penelitian Bioteknologi Perkebunan","volume":"9 2","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135168150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effect of Arbuscular Mycorrhizal Fungi (AMF) on agronomic characteristics of clove seedlings of superior local variety of East Halmahera 丛枝菌根真菌(AMF)对东哈马黑拉地方优良品种丁香幼苗农艺性状的影响

Menara Perkebunan: Jurnal Penelitian Bioteknologi Perkebunan Pub Date : 2023-10-24 DOI: 10.22302/e-journalmenaraperkebunan.v91i2.543

Fredy Lala, Hermawati Cahyaningrum, Yayat Hidayat, Bayu Suwitono

{"title":"The effect of Arbuscular Mycorrhizal Fungi (AMF) on agronomic characteristics of clove seedlings of superior local variety of East Halmahera","authors":"Fredy Lala, Hermawati Cahyaningrum, Yayat Hidayat, Bayu Suwitono","doi":"10.22302/e-journalmenaraperkebunan.v91i2.543","DOIUrl":"https://doi.org/10.22302/e-journalmenaraperkebunan.v91i2.543","url":null,"abstract":"Cloves (Syzygium aromaticum L.) have taproots but during the dry season the plants suffer from drought. Increasing the surface area of plant roots in the nursery is very important to produce clove plants with roots that have a high absorption of water and nutrients from the soil. The aim of this study was to determine the effect of arbuscular mycorrhizal fungi (AMF) on the growth of clove seedlings in the nursery and their nursery business. The research was conducted at a clove nursery in Telaga Jaya Village, South Wasile District, East Halmahera Regency, North Maluku from May to October 2020. The study used a Completely Randomized Block Design consisting of 4 treatments and three replications. The treatments were AMF doses: 0 g, 25 g, 50 g, and 75 g on each polybag. The results showed that the application of AMF increased plant height, number of branches, number of leaves, stem diameter, root length, and fresh weight of roots. East Halmahera clove nursery business had higher profits when using AMF than without AMF application.","PeriodicalId":476735,"journal":{"name":"Menara Perkebunan: Jurnal Penelitian Bioteknologi Perkebunan","volume":"62 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135321768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of cellulose from oil palm empty fruit bunches by fast delignification process with different solvents 不同溶剂快速脱木质素法研究油棕空果束纤维素的特性

Menara Perkebunan: Jurnal Penelitian Bioteknologi Perkebunan Pub Date : 2023-10-24 DOI: 10.22302/mp.v91i2.542

Firda Dimawarnita, Yora Faramitha, Haryo Tejo Prakoso, Indah Puspitasari, Doni Nugroho Kalbuadi, Dedy Prasetyo

{"title":"Characterization of cellulose from oil palm empty fruit bunches by fast delignification process with different solvents","authors":"Firda Dimawarnita, Yora Faramitha, Haryo Tejo Prakoso, Indah Puspitasari, Doni Nugroho Kalbuadi, Dedy Prasetyo","doi":"10.22302/mp.v91i2.542","DOIUrl":"https://doi.org/10.22302/mp.v91i2.542","url":null,"abstract":"Cellulose extraction typically begins with a delignification reaction using conventional methods, namely alkaline treatment. So far, the delignification process using alkaline treatment requires quite a long time, which is over 6 hours of the cooking process, so the cost to produce cellulose is quite large. The delignification approach using a variety of solvents is needed to answer the current problem, which is to shorten the reaction time. In this study, two types of solvents were carried out for the delignification process of EFB, namely NaOH and H2O2, while the time used for the delignification process was 15 minutes. Parameters of research observations included levels of lignin, cellulose, and hemicellulose.Extraction of cellulose from EFB using H2O2 and NaOH produced cellulose content of 52.76% and 66.46%, respectively. However, based on visual results of treatment using NaOH are still brown in color which indicates that lignin is still contained in EFB. The Fourier Transform Infra Red (FTIR)and X-Ray Diffraction (XRD) characterization results before and after delignified EFB showed that a peak of 3301.75 cm−1 dominated the functional group that show cellulose, with the highest crystallinity index of 12.43% in the H2O2 treatment. The results of Thermogravimetry Analysis (TGA)and Scanning Electron Microscopy (SEM) analyses showed that EFB before and after treatment were different, which can be observed from the loss of lignin structure and TGA values which began to degrade at 351.78°C. Therefore, based on this research the best solvent for fast delignification of EFB was H2O2 (only need 15 min).","PeriodicalId":476735,"journal":{"name":"Menara Perkebunan: Jurnal Penelitian Bioteknologi Perkebunan","volume":"138 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135321772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0