Ahmed Zakaria, Nessma Sultan, Nesreen Nabil, Mahitabe Elgamily
{"title":"Exosomes derived from bone marrow mesenchymal stem cells ameliorate chemotherapeutically induced damage in rats' parotid salivary gland.","authors":"Ahmed Zakaria, Nessma Sultan, Nesreen Nabil, Mahitabe Elgamily","doi":"10.1007/s10006-025-01331-9","DOIUrl":"10.1007/s10006-025-01331-9","url":null,"abstract":"<p><strong>Objective: </strong>A nanometer-sized vesicles originating from bone marrow mesenchymal stem cells (BMMSCs), called exosomes, have been extensively recognized. This study defines the impact of BMMSCs and their derived exosomes on proliferation, apoptosis and oxidative stress (OS) levels of CP-induced parotid salivary gland damage.</p><p><strong>Methods: </strong>BMMSCs were isolated from the tibia of four white albino rats and further characterized by flowcytometric analysis. BMMSCs-derived exosomes were harvested and underwent characterization using transmission electron microscopy (TEM), western blot analysis and BCA assay. Fifty-six healthy white albino male rats weighting from 200 to 250 g were allocated into 4 groups (n = 14); Group I, rats received phosphate buffered saline (PBS), group II, rats were intraperitoneally injected with CP, group III& IV received CP and after 3 days they were intravenously injected with either BMMSCs (group III) or BMMSCs-exosomes (group IV). Histological, and immunohistochemical studies using proliferating cell nuclear antigen (PCNA) were done after 7 and 14 days. The OS was measured using malondialdehyde (MDA) and apoptosis was measured by annexin V-FITC/PI.</p><p><strong>Results: </strong>BMMSCs and exosomes treated groups showed better histological features approximating the normal architecture of the control group. The percentage of PCNA positively stained cells were significantly higher in the exosomes treated group in comparison to all other groups. MDA assay test revealed that the exosomes were able to reduce the OS when compared to the cell-based therapy using BMMSCs. Annexin V revealed that BMMSCs-exosomes significantly reduced the percentage of apoptotic cells compared to other treated groups.</p><p><strong>Conclusions: </strong>BMMSCs-exosomes could improve the CP-induced cytotoxicity in rats' parotid salivary gland.</p>","PeriodicalId":47251,"journal":{"name":"Oral and Maxillofacial Surgery-Heidelberg","volume":"29 1","pages":"39"},"PeriodicalIF":1.7,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11742274/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143013971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Rushil Rajiv Dang, Balram Kadaikal, Sam El Abbadi, Branden R Brar, Amit Sethi, Radhika Chigurupati
{"title":"The current landscape of artificial intelligence in oral and maxillofacial surgery- a narrative review.","authors":"Rushil Rajiv Dang, Balram Kadaikal, Sam El Abbadi, Branden R Brar, Amit Sethi, Radhika Chigurupati","doi":"10.1007/s10006-025-01334-6","DOIUrl":"https://doi.org/10.1007/s10006-025-01334-6","url":null,"abstract":"<p><strong>Objective: </strong>This narrative review aims to explore the current applications and future prospects of AI within the subfields of oral and maxillofacial surgery (OMS), emphasizing its potential benefits and anticipated challenges.</p><p><strong>Methods: </strong>A detailed review of the literature was conducted to evaluate the role of AI in oral and maxillofacial surgery. All domains within OMS were reviewed with a focus on diagnostic, therapeutic and prognostic interventions.</p><p><strong>Results: </strong>AI has been successfully integrated into surgical specialties to enhance clinical outcomes. In OMS, AI demonstrates potential to improve clinical and administrative workflows in both ambulatory and hospital-based settings. Notable applications include more accurate risk prediction, minimally invasive surgical techniques, and optimized postoperative management.</p><p><strong>Conclusion: </strong>OMS stands to benefit enormously from the integration of AI. However, significant roadblocks, such as ethical concerns, data security, and integration challenges, must be addressed to ensure effective adoption. Further research and innovation are needed to fully realize the potential of AI in this specialty.</p>","PeriodicalId":47251,"journal":{"name":"Oral and Maxillofacial Surgery-Heidelberg","volume":"29 1","pages":"37"},"PeriodicalIF":1.7,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143013987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Benedikt Schneider, Dragan A Ströbele, Pascal Grün, Richard Mosch, Dritan Turhani, Constantin von See
{"title":"Smartphone application-based augmented reality for pre-clinical dental implant placement training: a pilot study.","authors":"Benedikt Schneider, Dragan A Ströbele, Pascal Grün, Richard Mosch, Dritan Turhani, Constantin von See","doi":"10.1007/s10006-024-01317-z","DOIUrl":"https://doi.org/10.1007/s10006-024-01317-z","url":null,"abstract":"<p><strong>Purpose: </strong>Precise implant placement is essential for optimal functional and aesthetic outcomes. Digital technologies, such as computer-assisted implant surgery (CAIS), have improved implant outcomes. However, conventional methods such as static and dynamic CAIS (dCAIS) require complex equipment. This study examined the usefulness of smartphone-based augmented reality (AR) for CAIS based on the value addition regarding angulation and positioning of pilot drillings and the potential for training dental students.</p><p><strong>Methods: </strong>An in vitro model was created to mimic dental scenarios using three-dimensional datasets. Smartphone technology and AR application (app)-based intraoral tracking were used for dCAIS. The app allowed real-time visualization of implant planning, superimposition of three-dimensional models, and alignment of surgical instruments. Forty dental students performed four pilot drillings each; two were performed freehand, whereas two were performed using the app. The angulation and position of the prepared implant bed preparation were statistically analyzed.</p><p><strong>Results: </strong>Implant angulation was significantly better in the AR-guided group than in the unguided group; however, no significant difference was observed in the implant position.</p><p><strong>Conclusions: </strong>Smartphone-based AR techniques for dCAIS are easily accessible in dental implantology. This may be advantageous for training dental students and potentially improving clinical outcomes, particularly the angulation of dental implants.</p>","PeriodicalId":47251,"journal":{"name":"Oral and Maxillofacial Surgery-Heidelberg","volume":"29 1","pages":"38"},"PeriodicalIF":1.7,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143013977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Paul Mario Capanni, Jeremey David McMahon, Bilal Aslam-Pervez, Iona Elizabeth Gray, David Young, Shiva Subramaniam, William Stuart Hislop, Ewen Thomson, Craig Wales, Mark Ansell, James Anthony McCaul
{"title":"Non-smoking, non-drinking, oral squamous cell carcinoma patients are a distinct and clinically significant group.","authors":"Paul Mario Capanni, Jeremey David McMahon, Bilal Aslam-Pervez, Iona Elizabeth Gray, David Young, Shiva Subramaniam, William Stuart Hislop, Ewen Thomson, Craig Wales, Mark Ansell, James Anthony McCaul","doi":"10.1007/s10006-025-01337-3","DOIUrl":"https://doi.org/10.1007/s10006-025-01337-3","url":null,"abstract":"<p><strong>Purpose: </strong>Carcinogenesis of oral squamous cell carcinoma (OSCC) has long been associated with exposure to tobacco smoke and alcohol consumption. Some centres have reported that non-smoking non-drinking (NSND) patients represent a significant and increasing proportion of OSCC cases with reports of poorer outcomes. Demographic characteristics are variably reported for this group and carcinogenesis is not fully understood. We present the largest cohort study to date in this subject area and sought to better understand demographics and survival.</p><p><strong>Methods: </strong>We interrogated 541 OSCC patients by retrospective analysis to assess risk factor status, disease characteristics and survival. Patients were categorised according to smoking and alcohol exposure with non-smoker (NS) status defined as less than five cigarettes per week with no history of use greater than this. Non-drinker (ND) status was defined as less than three standard drinks per week with no history of alcohol consumption greater than this. Those both NS and ND were categorised as NSND. Subsite, tumour stage and treatment were recorded along with evidence of cervical nodal and distant metastasis.</p><p><strong>Results: </strong>These patients were more likely to be female, older and present with early-stage disease. Tumour site was tongue, maxillary alveolus and buccal mucosa, at variance with the smoker drinker groups.</p><p><strong>Conclusion: </strong>NSNDs comprise a significant proportion our OSCC population. These patients were more likely to be female, older and present with early-stage disease. Tumour site was tongue, maxillary alveolus and buccal mucosa, at variance with the smoker drinker groups. Thus, NSNDs are a clinically distinct and significant group in oral cavity cancer management.</p>","PeriodicalId":47251,"journal":{"name":"Oral and Maxillofacial Surgery-Heidelberg","volume":"29 1","pages":"35"},"PeriodicalIF":1.7,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143013973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Admittance to the intensive care unit due to acute odontogenic cervicofacial infections; a single centre retrospective cohort study.","authors":"Dimitris Tatsis, Asterios Antoniou, Alexandros Louizakis, Konstantinos Paraskevopoulos, Athanassios Kyrgidis, Konstantinos Vahtsevanos","doi":"10.1007/s10006-024-01318-y","DOIUrl":"https://doi.org/10.1007/s10006-024-01318-y","url":null,"abstract":"<p><strong>Background: </strong>The aim of this study is to assess the outcome of the patients who required intensive care unit (ICU) admittance after surgical drainage of an odontogenic cervicofacial infection and identify the variables that are able to predict severe infection or a high possibility of complications.</p><p><strong>Patients and methods: </strong>This is a retrospective cohort study including all adult patients admitted to our hospital over the period 2011-2020 due to odontogenic cervicofacial infection and required ICU admittance. The study was approved by the hospital's scientific committee (no 814-9/8/2021).</p><p><strong>Results: </strong>51 patients were included (mean age 43.2 years). 11.7% of the patients had major comorbidities, such as diabetes or immunosuppression. At presentation, fever was recorded in 55% patients and trismus in 92.8%, with a mean delay from symptoms to hospital admission at 3 days. 23% of patients had a post-extraction infection, 61% pericoronitis and 15% other odontogenic infections. The mean duration of hospitalization was 9.16 days whereas the mean ICU length of stay was 3.76 days. 84.3% of patients showed improvement, 13.7% deteriorated with a need of reoperation and one death was reported. ICU length of stay was associated with an 89% reduced rate when positive microbe identification was feasible, whereas rate of complications was 4-fold higher in patients with prolonged ICU length of stay.</p><p><strong>Conclusions: </strong>Patients with deep cervicofacial odontogenic infections who require ICU management have a favourable clinical outcome. A minority of these patients will not improve in a short time frame or develop complications which require further management.</p>","PeriodicalId":47251,"journal":{"name":"Oral and Maxillofacial Surgery-Heidelberg","volume":"29 1","pages":"34"},"PeriodicalIF":1.7,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142984923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Ideal mentolabial sulcus depth in long-face, short-face, and normal-face in female individuals.","authors":"Marzie Mazhari, Atefe Rekabi, Nastaran Atashkar, Zahra Khayami","doi":"10.1007/s10006-025-01336-4","DOIUrl":"https://doi.org/10.1007/s10006-025-01336-4","url":null,"abstract":"<p><strong>Purpose: </strong>The harmony between the lips and the chin is an essential parameter in facial esthetics, and the mentolabial sulcus (MLS) represents the level of this harmony. This study aimed to assess the effect of MLS depth on facial profile attractiveness in long-face, short-face, and normal-face Iranian female individuals.</p><p><strong>Methods: </strong>An observational study with a questionnaire was conducted, and a profile photograph of an Iranian female with class I occlusion was altered to create long-face, normal-face and short-face profiles with MLS depth ranging from 1 to 12 mm. Sixty-five lay people and 24 orthodontists rated the attractiveness of images using a 7-point Likert scale. Data were analyzed by independent t-test and Spearman's correlation coefficient using IBM SPSS Ver. 22.</p><p><strong>Results: </strong>The ideal depth of MLS ranged from 5 to 7 mm for normal-face and short-faced and 6-8 mm for long-face Iranian females. The acceptable MLS depth was found to be up to 8 mm in normal-face and up to 10 mm in long-face and short-face females. normal-face images acquired the maximum score of attractiveness, followed by short-face images, while long-face images were the least attractive according to both observer groups. There was general agreement amongst orthodontists and lay people scoring process; however, orthodontists gave significantly higher scores for normal face images than lay people.</p><p><strong>Conclusion: </strong>The optimal MLS depth in different facial types should be considered in treatment planning for orthognathic surgery and genioplasty.</p>","PeriodicalId":47251,"journal":{"name":"Oral and Maxillofacial Surgery-Heidelberg","volume":"29 1","pages":"33"},"PeriodicalIF":1.7,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142980151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Bone expansion as a horizontal alveolar ridge augmentation technique: a systematic review and meta-analysis.","authors":"Ali Azadi, Parham Hazrati, Ashkan Tizno, Fatemeh Rezaei, Alireza Akbarzadeh Baghban, Reza Tabrizi","doi":"10.1007/s10006-025-01335-5","DOIUrl":"https://doi.org/10.1007/s10006-025-01335-5","url":null,"abstract":"<p><strong>Purpose: </strong>Bone expansion is one of the quickest, simplest, and most reliable methods of alveolar ridge augmentation for implant placement. This systematic review is designed to investigate the outcomes of the bone expansion technique for horizontal ridge augmentation.</p><p><strong>Methods: </strong>The protocol of study has been prospectively registered into PROSPERO (CRD42023414686). Pubmed, Embase, Web of Science, and Scopus databases were searched. Human original articles utilizing different bone expansion procedures for horizontal ridge augmentation without the use of bone materials or combined with vertical ridge augmentation techniques were included in the study. Qualitative and quantitative synthesis have been conducted. I-squared was used to assess heterogeneity. Meta-regression, forest plots random-effect model, and weighted mean difference were used for analysis. Leave-one-out influence analysis for finding outliers. Egger's publication bias test and Funnel plots were used to address publication bias.</p><p><strong>Results: </strong>3291 records were identified. Ten articles were included in this study after screening. The survival and complication rates of implants placed simultaneously with bone expansion was 100% 100% (95% CI: 99-100%) and 0% (95% CI: 0-1%), respectively. The meta-regression revealed that by each month passing from the implant placement the survival probability would be decreased significantly by 0.08% (95% CI: 0.009-0.15%; P = 0.03). The weighted mean ridge width difference before and after the osseodensification was 1.55 mm (95% CI: 0.92-2.17 mm).</p><p><strong>Conclusion: </strong>Based on the study's limitations it can be concluded that the bone expansion procedure can be considered an efficient and safe method regarding the survival and complication rates of placed implants.</p><p><strong>Trial registration number: </strong>N/A.</p>","PeriodicalId":47251,"journal":{"name":"Oral and Maxillofacial Surgery-Heidelberg","volume":"29 1","pages":"32"},"PeriodicalIF":1.7,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142980133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Perioperative changes in anxiety and comfort in third molar extraction patients sedated with midazolam.","authors":"Kazuhiro Murakami, Satoshi Horita, Motokatsu Tsuyuki, Akihito Moriyasu","doi":"10.1007/s10006-024-01326-y","DOIUrl":"https://doi.org/10.1007/s10006-024-01326-y","url":null,"abstract":"<p><strong>Purpose: </strong>This study aimed to analyze changes in anxiety and comfort levels at each perioperative stage during third molar extraction under single-agent intravenous midazolam sedation, and to clarify how these conditions at each perioperative stage affect postoperative satisfaction.</p><p><strong>Patients and method: </strong>115 who requested extraction of 1 ~ 4 third molars under single-agent intravenous midazolam sedation were targeted. These patients were administered a questionnaire survey one week after surgery. The questionnaire's five items (Anxiety before surgery, Comfort during surgery, Comfort after surgery, Amnesia, and Satisfaction) were evaluated using a visual analog scale (VAS). We investigated the correlation between each of the eight parameters which added age, operation time, and total amount of midazolam, to the VAS score. Furthermore, we compared each eight parameters according to the number of extracted wisdom teeth and sex differences.</p><p><strong>Results: </strong>Regarding the correlation among each parameter, low preoperative anxiety were weak and positively correlated with reduced operation time reduced midazolam dosage, and an increase in postoperative comfort. All parameters influenced the patient's satisfaction level. Those levels were significantly affected by preoperative reassurance (r = 0.4402, p < 0.01), postoperative comfort (r = 0.5522, p < 0.01) amnesia (r = 0.5741, p < 0.01), and intraoperative comfort (r = 0.7578, p < 0.01). Each parameter had no significant difference depending on the number of teeth extracted. In comparison between men and women, only preoperative reassurance was significantly lower in women than in men (p<0.05).</p><p><strong>Conclusion and relevance: </strong>This sedation effectively managed preoperative anxiety and improved postoperative satisfaction. To obtain good sedative condition, Preoperative anxiolytics are recommended. This sedation is considered particularly effective in women.</p>","PeriodicalId":47251,"journal":{"name":"Oral and Maxillofacial Surgery-Heidelberg","volume":"29 1","pages":"31"},"PeriodicalIF":1.7,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142980157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Anthony D DiPalma, Hasan Zia, Brandon Goodwin, Usmaan Al-Shehab, Anusha Bharadia, Jared Goldfarb
{"title":"Efficacy of absorbable vs. non-absorbable patient-specific, 3D-printed implants for the treatment of facial bone fractures: a systematic review and meta-analysis.","authors":"Anthony D DiPalma, Hasan Zia, Brandon Goodwin, Usmaan Al-Shehab, Anusha Bharadia, Jared Goldfarb","doi":"10.1007/s10006-024-01327-x","DOIUrl":"https://doi.org/10.1007/s10006-024-01327-x","url":null,"abstract":"<p><strong>Objective: </strong>This systematic review and meta-analysis compares the efficacy and complication rate of absorbable versus non-absorbable 3D-printed, patient-customized, maxillofacial implants in facial trauma patients.</p><p><strong>Data sources: </strong>A comprehensive search of four databases (PubMed, Scopus, Web of Science, and Cochrane) was conducted.</p><p><strong>Methods: </strong>A systematic review and single-proportion meta-analysis was conducted employing PRISMA guidelines. A comprehensive search of four databases (PubMed, Scopus, Web of Science, and Cochrane) yielded a total of 4087 results. After removing duplicates, 16 articles underwent full-text analysis, with 13 meeting the inclusion criteria. The inclusion focused on primary clinical data involving 3D-printed, patient-specific implants for facial bone fracture restorations. Exclusion criteria removed studies without full text, ongoing studies, animal studies, and studies not utilizing CAD/CAM for their implants.</p><p><strong>Results: </strong>A total of 114 patients underwent insertion of 3D-printed implants. Patients receiving non-absorbable implants had a success rate of 84% (95% CI: 74-91), with complications in 12 patients. Patients receiving absorbable implants achieved a 100% success rate (95% CI: 0-100), with zero complications.</p><p><strong>Conclusion: </strong>The study suggests absorbable 3D-printed implants provide superior results with fewer complications compared to non-absorbable 3D-printed implants for the treatment of facial fractures.</p><p><strong>Clinical trial number: </strong>Not applicable.</p>","PeriodicalId":47251,"journal":{"name":"Oral and Maxillofacial Surgery-Heidelberg","volume":"29 1","pages":"30"},"PeriodicalIF":1.7,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142972639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Natália V Bisatto, Betina B Crescente, Guilherme G Fritscher, Maria M Campos
{"title":"Sleep disorders in individuals with dentofacial deformities: a preliminary study on the relationship with sex and serotonin levels.","authors":"Natália V Bisatto, Betina B Crescente, Guilherme G Fritscher, Maria M Campos","doi":"10.1007/s10006-025-01333-7","DOIUrl":"10.1007/s10006-025-01333-7","url":null,"abstract":"<p><strong>Purpose: </strong>It has been suggested that dentofacial deformities (DFD) can impair sleep quality. This pilot study aimed at evaluating sleep disorders in individuals with DFD before orthognathic surgery, correlating the clinical findings with salivary biomarker levels.</p><p><strong>Methods: </strong>This cross-sectional study enrolled ten males and ten females with DFD diagnoses under orthodontic treatment preceding orthognathic surgery. The participants responded to the Pittsburgh Sleep Quality Index (PSQI), the Epworth Sleepiness Scale (ESS), and the Fletcher and Luckett Sleep Questionnaire (FLSQ). Obstructive sleep apnea (OSA) was examined by the Home Sleep Apnea Test (HSAT). The salivary levels of interleukin-1β (IL-1β), glutamate, and serotonin were measured.</p><p><strong>Results: </strong>85% of individuals presented PSQI and FLSQ scores indicative of sleep alterations. Females had higher scores in part 2 of the FLSQ instrument, referring to sleepiness-associated complaints. HSAT analysis revealed a low number of symptomatic OSA individuals, with three males demonstrating altered oxygen desaturation rates. There was a significant negative correlation between the salivary levels of serotonin and the FLSQ results.</p><p><strong>Conclusions: </strong>Individuals with DFD diagnosis showed poorer sleep quality, which is likely independent of sex and OSA diagnosis, and negatively correlated with salivary levels of serotonin.</p>","PeriodicalId":47251,"journal":{"name":"Oral and Maxillofacial Surgery-Heidelberg","volume":"29 1","pages":"28"},"PeriodicalIF":1.7,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142956508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}